Problem with cloning p53-suggestions for RT-PCR - (Aug/07/2006 )
I am trying to clone full length p53 from a RNA pool isolated from chicken lymphocytes. I used ambion RNAqeous kit.This RNA is rich in GC content. Even with DMSO, I do n't get any bands. I checked the integrity with actin primers which work just fine. Also I see a faint band when I use internal primers for p53. Any suggestions?
you try using a gene specific primer to prime your RT (rather than the oligodT or random hexamers)....