problem with pcr product - pcr problem (Feb/04/2002 )
My PCR reaction worked but I got another problem. After PCR, I checked my product but gel electroporesis.
Yes, I got my product but also other three unrequired bands. And I have to run gel again to seperate the required band, then sequencing. I think my annealing temperature was very low, 53 C. And I'm afraid tha tI 'll get nonspecific product. Should I increase the annealing temp. to 58 -59 C to get rid of these nonspecific bands
Based on the information in the previous post alone: Yes. Try 60 C, it's a standard annealing temperature.