Should Taq polymerase be resuspended before use? - (Mar/18/2008 )

Sorry my english is not good, I hope you understand me!...Recently I used a new Taq tube that was stored at -80°C. I put it at -20°C while I prepared PCR mixture. When I pipette the Taq I saw two phases, glicerol below. That is normal? I never observed that. I heard that Taq should not mix by vortex. Probably is better resuspend it by pypetting?
Thanks,
Ml21

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Or flick the tube. There was a discussion and poll on this topic some time ago with pcr reactions mixes (this one).

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Thank you very much for your answer!! I saw the discussion about pcr reaction mixes...it's very interesting, it seems that there are many ways to do the same! My specific question was about the Taq because in my last PCR I had no product. I hope that it was a pipett problem related with a non homogeneosu Taq solution (probably because it was stored at -80°C) Next time, I'll flick the Taq tube as you suggest.
Thanks again,
Ml21

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PS I would not store taq at this temperature, -20°C should be enough. Don't know if the enzyme stands -80°C, but if phases separate and the aqueous part with the taq freezes, then the enzyme is dead.

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