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Calculating correct melting temperature for primers - (Jun/21/2005 )

How can I know the exact Tm of my oligos. Diffent softwares give different results?

-viskamw-

Check the parameters within the software. The melting temperature is highly dependant on the salt concentration in the reaction, I tend to find if you customise the salt parameter in the software it will come out with approximately the same Tm.

Cheers,

Scott

-Scott-

hi
tm of oligos may be calculated easyly at this web page. Moreover this <ebpage explains the formula used...

http://www.in-silico.com/tm.php?formula=show

-fred_33-