how long a primer should I use? - (Apr/10/2005 )
hi, I am cloning from cDNA but failed. As I go through literature, it occured to me that people have used some pretty long primers for this kind of work, I just wonder how long should the primer be to be able to get products? Though other factors may also affect the result of PCR, I want to make sure that my primers, in the first place, is not too short for it.
thansk!!
-tfcheng-
Hi,
What is the length of your existing primers and how big is your product? The problem may not lie in primer length. Primers around 18-25 should work for most PCRs.
-pcrman-