southern hybridizaion with 5' end labelled primer - (Jan/16/2006 )
i was trying to hybridize a genomic dna (bglI digested) blot with a primer(Tm 6o degree in 1M Na+)
labelled with p32 (5' end labelling).
at 6o degree i m getting no signal and at 50 degree i m getting a dark background.
Have you about trying a temp in between? 10C is fairly large jump for a primer (think about PCR).