Does anyone know how to design LUX primers - (Nov/11/2007 )
I was wondering is there anyone out there have experiences on LUX primer design (from Invitrogen)?
From invitrogen website, seems that this primer can do both Real time and melt curve analysis. I have a gene that doesn't fit what their program design, so I would like to have it manually design.
Hope this finds you well. I've tried LUX primers for the work I'm doing. However, when I asked them for the software to design the primers (as I couldn't find ones that fit the region I gave them), they said that they don't provide to customers outside the USA and that I should use the web tool provided. What I had to do in the end was to provide a much larger sequence and try and work something out. Sorry I couldn't be of more help.
Best wishes and good luck!
Hi Stand Free,
Thank you for your reply. I think I have to spend more time on reading and design of the primers.