cDNA PCR - Amplifying region using PCR from cDNA library (Apr/19/2002 )
I'm having problems getting a clean product whilst trying to PCR from cDNA libraries. I'm sure the primer annealing temps. are good and the PCR seems to work well on a positive control of pure plasmid DNA. I'm also sure that my gene is expressed in this library.
Any ideas ?? Thanks in advance
Please try a nested PCR using the sequencing primers for the cDNA library and the specific primers for the gene. This may help you.