cassette pcr - (Aug/23/2007 )
A paper I am reading says that the construct was made by cassette pcr; however, I am having difficulty determining exactly what this is, and how it could be used for this paper.
Paper: Science, vol 291, 2001, pg 884
Can anyone explain?
-brich-
QUOTE (brich @ Aug 23 2007, 02:18 PM)
A paper I am reading says that the construct was made by cassette pcr; however, I am having difficulty determining exactly what this is, and how it could be used for this paper.
Paper: Science, vol 291, 2001, pg 884
Can anyone explain?
Paper: Science, vol 291, 2001, pg 884
Can anyone explain?
could you write the whole sentences or paragraph please?
I'm not sure, I thinks it like.. you obtain different PCR products.
-aztecan princess-
I searched a little and this is what I found:
The primers align to conserved sequences, usually the 59-base element family of recombination sites and amplify a whole “cassettes” containing complete open reading frames, majority associated with ribosome binding sites.
This way, unknown sequences can be cloned and sequenced.
Check this out:
-aztecan princess-