RT-PCR primer design - RT-PCR primer design...need ur help (Oct/24/2008 )
Can someone tell me how to design a RT-PCR primer?
I am the first one(first time) to do it in my lab.
I got a couple of informations from the net but some are
just to difficult to understand for a beginner like me.
What is the most important factor (Max score, Total score
,E-value...) to look when you blast the amplicon sequence
(or the primers)against genome database.
Thank you for your help.
Primer3 will help the beginner to pick up primer for nomal PCR.