Hi!

I need gelatin for my PCR, but I could not find any for molecular biology. Where to buy and which one? Help me!

-Noora-

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PCR buffers used to contain gelatin as a bulking agent to concentrate protein, but it has gradually been left out because it interferes with DNA sequencing gels. Gelatin may be used as a stabilizer at 0.001% (w/v). Fish gelatin is generally used to avoid potential mammalian DNA contaminants. Check your Sigma catalog.

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dear noora,
please do let me also know if u come across something specifically regarding a mol bio grade gelatin. so far i am planning to use routine gelatin after double autoclaving. have u tried it ? and if so then waht were the results?

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