RT-PCR Question.. - (Dec/19/2006 )
I know my doubt is stupid..
Can anyone visualize cDNA after RT rxn (I mean before the PCR part in the two-step RT-PCR expt) on agarose gel.. If CANT, why NOT!! b/c cDNA is double stranded, aint it!!!
Help is needed!!!!!
You might be able to visualise the cDNA, but what do you want to do with it? If you're going to PCR, just do the reaction and see what you get at the end. If you are looking at other things, I'd suggest you trust that your RT has worked (after doing the usual controls), do your next step, and go from there.