how much total RNA do you use for RT-PCR - (Jul/20/2005 )

hi guys?
how much total RNA do you use for RT-PCR?

-hsm142-

I usually use 0.5-1 ug total RNA.

-rajcell-

I usually use 1 ug as well.

-haringsh-

QUOTE (hsm142 @ Jul 20 2005, 07:03 AM)

hi guys?
how much total RNA do you use for RT-PCR?

I use 3-5µg

-Walid-

2 - 4 ug

-Pallas-

250ng - 1ug

-John Buckels-

usually 1 ug is enough

if you prepare a probe, you can use even 4 or 4 ug of total RNA

-Mrad-