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RT-PCR - not qPCR! urgent please - how to choose primers (Oct/06/2006 )

My question:
When I pick the primers for the RT-PCR, should they match the unspliced mRNA or the CDS (the spliced variant that will be translated and starts with the ATG)???


It depends upon if the cDNA was synthetized from mature mRNAs or total mRNAs. If you used poly-A primers for the cDNA, the cds match should work. Good luck!


primerbank may be useful too.


thank you for the advice!