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PCR Related Discussions
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oligo(dt) 15 vs random primers - (reply: 1)
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Multiple bands in eluted PCR product - (reply: 1)
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having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 3)
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Problems with two step RT-PCR - (reply: 1)
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Getting genomic DNA for PCR - (reply: 4)
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primer dilutions - (reply: 4)
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Genotyping PCR primers - (reply: 1)
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Conventional PCR - (reply: 1)
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PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
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PCR product loss on cleanup - (reply: 2)
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MSP Primers not working - (reply: 4)
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Primers too dilute - (reply: 1)
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Amplification curves jagged - (reply: 1)
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Cloning HELP- Possible Primer issues - (reply: 6)
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No PCR product in site-directed mutagenesis - (reply: 4)
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Giardia/Crypto real time PCR late amplification - (reply: 1)
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How to dilute the template DNA for PCR - (reply: 7)
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After I run my pcr on agarose the DNA is still in the well - (reply: 3)
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Need help for PCR - (reply: 5)
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PCR primer pairs - university exam - (reply: 7)
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unespecific band PCR from mouse genomic DNA - (reply: 9)
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Help with primer design through PRIMER3 - (reply: 4)
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Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
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Normalizing to negative control primers for ChIP qPCR - (reply: 3)
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Plasmid vs cDNA amplification by PCR - (reply: 2)
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Tm of primers - prediction and verification - (reply: 1)
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PCR cloning...help! - (reply: 5)
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RT-PCR contamination in negative control. - (reply: 1)
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PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
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how to design primers for 16sr RNA - (reply: 21)
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how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
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Help with primer design - (reply: 3)
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Primer Tm calculation. - (reply: 1)
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low concentration of purified PCR product - (reply: 2)
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PCR smearing and...problems! - (reply: 7)
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Running Primers on Agarose Gel - (reply: 6)
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PCR product now not getting with same primers and other PCR conditions - (reply: 4)
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universal primers for PCR ribotyping - (reply: 6)
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will the template of PCR be added A ? - (reply: 2)
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Colony PCR not working - (reply: 4)
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Primers for Introduction of new restriction sites to a vector - (reply: 3)
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Primer design: Tm above or below Ta? - (reply: 5)
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Oligo primers for shRNA construction - (reply: 1)
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Design primers for expression cloning - (reply: 8)
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Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
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primer dilution query - (reply: 2)
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Real Time PCR polymerase enzyme - (reply: 3)
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Estimating size of PCR products in a agarose gel - (reply: 4)
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Sequencing of PCR product - (reply: 4)
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PCR contamination problem - (reply: 2)
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Using BLAST to check primers - (reply: 1)
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How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
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What is the function of a third primer in a PCR mix?? - (reply: 2)
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How can we design a primer ? step by step method - (reply: 4)
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cDNA synthesis or RT-PCR - (reply: 3)
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PCR on cDNA and SGamplification??? - (reply: 1)
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EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
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real time PCR protocol - controls? - (reply: 2)
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Long primers (60bp) and 5kb PCR product problems - (reply: 5)
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Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
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SOS: new real time PCR machine needed.... which one? - (reply: 5)
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which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
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My product of PCR haven't the lenght right after purification....... What - (reply: 2)
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Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
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PCR and UV spectrophotometry - (reply: 3)
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RT-PCR in a single tube - (reply: 3)
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why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
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annealing temperature for 3 primer pairs? - (reply: 4)
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PCR Reaction - (reply: 3)
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Impact of primer sequence on TA cloning - (reply: 3)
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problems with Bisulfite PCR - (reply: 1)
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low pcr product - (reply: 3)
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overlapping PCR protocol - (reply: 12)
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PCR to amplify my insert not working - (reply: 6)
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Problem with PCR amplification- high 3' stability - (reply: 3)
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Home made real-time PCR problem - (reply: 3)
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High RNase concentration interfering with PCR? - (reply: 8)
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Pcr primers - (reply: 2)
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PCR failure from yeast genomic DNA - (reply: 1)
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failure of PCR from yeast genomic DNA - (reply: 1)
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RT-Q PCR primer design question - (reply: 2)
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high MW dimers - (reply: 2)
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Strange RT-PCR amplification plots - (reply: 9)
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How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
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What products will I get from this pcr? - (reply: 1)
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PCR buffer without Tris - (reply: 1)
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PCR Water - (reply: 2)
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multiplex rtqPCR and custom primer/probe design - (reply: 2)
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Which Brand of real-time PCR machine - (reply: 6)
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Problem with PCR - large extra bands - (reply: 3)
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big differnece in my primers Tm - (reply: 2)
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Designing primers for the ORFs - (reply: 9)
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Two-Step PCR - (reply: 2)
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Gradient PCR - (reply: 2)
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PCR with phophorylated primers - (reply: 1)
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Problem with New qPCR primers - help! - (reply: 6)
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Designing primers for miRNA located on minus strand. - (reply: 2)
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Plasmid sequencing without primers - (reply: 1)
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Smallest insert in PCR - (reply: 6)
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troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
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loop-mediated amplification - (reply: 1)
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Blunt end PCR product ligation - (reply: 1)
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Help with high Ct values for Real-Time PCR - (reply: 1)
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Genotyping by allele specific PCR - (reply: 6)
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RT-PCR primers and the poly(A) tail - (reply: 2)
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NO Insert found in the Colony PCR - (reply: 15)
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No band in PCR - (reply: 5)
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overlapping primer - (reply: 1)
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PCR primers help - (reply: 5)
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PCR smear after one-month storage - (reply: 2)
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Primer dimers - (reply: 2)
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RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
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Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
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Simple question of dNTP's for PCR - (reply: 5)
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Need help for PCR for AT rich gene - (reply: 3)
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How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
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Primer validation and PCR efficiency - (reply: 4)
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How to calculate the price of PCR test per sample? - (reply: 6)
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Contamination in negative control of PCR (No template control) - (reply: 6)
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cloning with nested pcr - (reply: 3)
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What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
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PCR is amplifying non-specific fragment - (reply: 6)
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ChIP amplification problems - (reply: 1)
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real time RT-PCR problems - (reply: 2)
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should I design primers spanning both intron and exon - (reply: 2)
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RT-PCR - (reply: 1)
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RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
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Template DNA (plasmid) concentration for PCR - (reply: 4)
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PCR with a very long and a short primer - (reply: 5)
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Ligation of 16kb PCR product - (reply: 5)
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Multiplex PCR does not work - (reply: 3)
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Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
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Problem with genomic PCR (2.4 kb) - (reply: 1)
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Comparison between two primers pairs? Is it possible? - (reply: 3)
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Rt-PCR primer design - (reply: 7)
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Length of non-binding sequence in primers with RE site - (reply: 6)
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ChIP-PCR: Amplification in IgG negative control - (reply: 1)
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Getting a smaller PCR product than required - (reply: 6)
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Difficult sequences in PCR - (reply: 2)
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why is real time pcr curve like this? - (reply: 3)
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PCR inconsistency - (reply: 2)
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Orientation of primers - (reply: 2)
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PCR, neg control & no. of cycles - (reply: 2)
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Strange PCR result from genomic DNA - (reply: 3)
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Bright bands of PCR products stick in gel wells - (reply: 1)
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methylation analysis with MS-HRM (primer design) - (reply: 1)
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whole plasmid pcr? - (reply: 6)
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Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
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salts in PCR - (reply: 4)
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Strange amplification plot - (reply: 4)
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Conventional PCR of different dilutions - (reply: 1)
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primer resuspension - (reply: 2)
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Principles of overlap PCR - (reply: 7)
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ligation of pcr product into expression vector - (reply: 4)
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Unable to PCR!!!! - (reply: 3)
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Help designing primers with restriction sites - (reply: 3)
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Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
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Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
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Abnormal amplification curve - (reply: 1)
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Problem with Semiquantitative PCR - (reply: 4)
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Advice on optimising bisulfite PCR sought - (reply: 2)
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Help: I am loosing the DNA during PCR purification - (reply: 7)
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PCR primer with no extra DNA end - (reply: 5)
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PCR colonies of Pichia pastoris - (reply: 1)
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ROX reference dye for quantitative RT-PCR - (reply: 1)
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PCR problem - (reply: 9)
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why PCR product smeared? - (reply: 2)
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Tm calculation for primers with RE sites and overhangs - (reply: 4)
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silincing box, no amplification - (reply: 3)
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Comparative semiquantitative RT-PCR - (reply: 2)
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Bands in my PCR Controls - (reply: 3)
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cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
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Strange band in colony PCR - (reply: 2)
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In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
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Multiplex PCR - (reply: 12)
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Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
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PYO pcr stopped working. - (reply: 1)
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Bands seen with qPCR missing in regular PCR - (reply: 5)
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checking msp primers - (reply: 2)
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PCR product one day, none the next day - (reply: 3)
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PCR on colony - (reply: 10)
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PCR reaction ISSUES???? need help - (reply: 4)
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PCR using CDNA as template - (reply: 2)
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RT-PCR doesn't work with all RNA used - (reply: 1)
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Need help amplifying repeating sequence. - (reply: 5)
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Real Time PCR 101...help! - (reply: 1)
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RT-PCR primer design for full length cDNA cloning - (reply: 6)
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problems with PCR confirmation of insert . HELP ! - (reply: 1)
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Another primer dimers problem - (reply: 23)
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Colony PCR Problem!!!! - (reply: 5)
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using PCR product as standard template - (reply: 3)
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PCR kit - (reply: 7)
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Make additional cycles with an already finished pcr product - (reply: 3)
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Amplification in water control but not in samples - (reply: 3)
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PCR products form with DNA, but not with c-DNA - (reply: 2)
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question about pcr amplification efficiency? - (reply: 1)
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DNA polymerase: recombinant or native? - (reply: 3)
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pcr clean-up fail? - (reply: 1)
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MSP U primers very difficult to amplify... - (reply: 4)
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No gene amplification, 18s amplification is fine - (reply: 1)
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ChIP-qPCR gives odd amplification plot - (reply: 5)
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RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
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sequential cloning of multiple PCR products - how to do? (reply: 3)
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SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
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Primer design - (reply: 3)
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Tomato Actin PCR+Images included - (reply: 2)
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single primer PCR - (reply: 5)
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Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
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cDNA synthesis + amplification - (reply: 2)
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Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
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LacZ PCR Problems - (reply: 1)
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cells stably expressing T7 RNA polymerase - (reply: 1)
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Primer concentration - (reply: 4)
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nested PCR with low target DNA? - (reply: 2)
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RT-PCR calibrator - (reply: 2)
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RNA - extraction and pcr (reply: 1)
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problem in pcr - (reply: 2)
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Can someone recommend me a virtual PCR software? - (reply: 6)
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Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
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Primer 3' mismatch - Strange experience (reply: 9)
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real-time PCR after restriction enzyme digestion - (reply: 6)
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wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
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Adding loading/tracking dye to PCR mix? - (reply: 10)
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PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
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DNA polymerase with single G 3'-end overhang activity - (reply: 3)
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Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
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relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
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how to get rid of non specific bands in PCR - (reply: 1)
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PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
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PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 1)
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which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
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PCR cloning problem - could not get colony (reply: 4)
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pcr contamination - (reply: 4)
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Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
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Large Non-specfic bands in PCR - (reply: 3)
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PCR clonning - (reply: 3)
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Difference between PCR/cloning DNA in plasmids - (reply: 2)
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2 bands in blue colony PCR - (reply: 5)
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Primer dilution --> problem.. - (reply: 16)
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Help! Smear in Real-Time PCR Product - (reply: 1)
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alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
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Sybr green RT-qPCR primer - (reply: 1)
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Sybr green RT-qPCR primer - (reply: 1)
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primer design - microalgae (reply: 2)
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Designing primers - What is more important Ta or primer's Tm - (reply: 3)
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Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
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real time rt PCR - bloodyurine - inhibition - (reply: 1)
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PCR yield in ng/ul - (reply: 1)
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No Amplification in PCR - (reply: 4)
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Real time PCR and percentage loss - (reply: 1)
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Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
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Problem with smear in PCR with certain templates - (reply: 4)
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Real time PCR in the presence of heme - (reply: 3)
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issues in PCR amplification - (reply: 2)
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Problems with PCR in general - (reply: 1)
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Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
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how to overcome primer contamination - need protocol (reply: 11)
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RT-PCR primer design - Primer design and evaluation (reply: 7)
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CHIP analysis by PCR - (reply: 4)
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STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
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Primer,probes dilution - (reply: 3)
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Splice varient quantification in RT real time PCR? - (reply: 1)
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negative control for msp pcr - (reply: 2)
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Calculating geometric mean for real-time PCR - (reply: 5)
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template transfer? - how does the rna polymerase switches template? (reply: 1)
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PCR Gel nonspecific band - (reply: 13)
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semiquantitative pcr - (reply: 1)
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Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
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PCR: Cloning Primers - (reply: 2)
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PCR product - (reply: 5)
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pcr amplify an insert in plasmid? - (reply: 2)
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Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
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Exon Spanning PCR Primers - (reply: 1)
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Colony PCR - (reply: 1)
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Primer sequences - (reply: 1)
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Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
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smears in PCR products (bisfulite treated DNA) - (reply: 2)
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PCR Primers - (reply: 2)
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Cleaning up RT-PCR product before sequencing - (reply: 1)
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Template Amount for PCR Amplification - (reply: 6)
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How to blast methylation specific and unspecific primers - (reply: 1)
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RT-PCR cDNA synthesis - (reply: 6)
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problems amplifying from cDNA - (reply: 4)
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Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
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No DNA after PCR purification with QiaQuick? - (reply: 4)
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restriction digestion against colony PCR - (reply: 1)
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How to check primers are of correct sequence or not? - (reply: 2)
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Primers... - (reply: 1)
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When are primer dimers a problem? - (reply: 1)
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Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
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PCR: always got band in the negative control - (reply: 8)
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primer with higher melting temperature - (reply: 2)
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Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
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Problems designing primers for BSP - (reply: 2)
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qPCR primer design - possible off-target (reply: 1)
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Primer dimer formation and real-time PCR - (reply: 7)
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Identify PCR products - (reply: 3)
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PCR genomic DNA using cell lysate - (reply: 1)
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Maximum size of overhangs in PCR - (reply: 6)
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Colony PCR doesn't work anymore - (reply: 5)
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RT-PCR t-test, and ANOVA - (reply: 1)
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Primer Design Urgent! - (reply: 1)
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PCR basics - I'm new to all things lab and need some help! (reply: 3)
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Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
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PCR of microRNA first strand cDNA - (reply: 1)
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how to know my primer's sense ??? - help ! (reply: 2)
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smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
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High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
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problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
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intron spanning primers for non model organisms - (reply: 2)
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Primer design - (reply: 2)
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Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
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qPCR amplification interference fixed with freezing - (reply: 6)
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Suggest me a mastermix for conventinal PCR... - (reply: 11)
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software for quantifing PCR product band - (reply: 1)
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Multiple bands from purified PCR product - (reply: 3)
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Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
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Doing PCR on Nebulized DNA - (reply: 2)
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Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
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RT-PCR - (reply: 9)
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help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
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inverse pcr - (reply: 2)
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Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
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Primer seq - (reply: 2)
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Urgent: should you dry PCR product - (reply: 13)
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PCR product size - (reply: 6)
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amplification plot raises to early - What is the explanation for this problem? (reply: 1)
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oligo dt and random primer - (reply: 3)
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mRNA Search for RT-PCR (U to T) - (reply: 2)
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First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
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PCR Mastermix - (reply: 4)
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PCR triplicates versus one reaction - differences? (reply: 17)
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Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
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Do low binding 0.2ml PCR tubes exist? - (reply: 3)
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Primers and self and hetero dimers - (reply: 1)
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Really weird amplification curves - (reply: 1)
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Primers for qPCR - (reply: 3)
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Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
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RT-PCR primer - (reply: 3)
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PCR problem - basic PCR for plasmid amplification (reply: 2)
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Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
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mutation using overlapping PCR, what's the success rate? - (reply: 3)
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Unpredictablity of PCR product - (reply: 5)
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total cDNA amplification by PCR - (reply: 2)
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Detection limit of a conventional PCR - (reply: 2)
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PCR for MULTIPLE mutagenesis - (reply: 4)
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Using Sybr green in realtime pcr - (reply: 1)
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IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
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Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
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Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
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Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
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Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
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Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
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Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
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Digested PCR product migrate slower than uncut - (reply: 8)
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Left polymerase above fridge for more than 1 week - still working? (reply: 3)
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PCR question - (reply: 2)
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positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
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2 rounds PCR got problem - (reply: 2)
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PCR help minus strand - Primer design (reply: 1)
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chloramphenicol storage and amplification - (reply: 3)
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Smear in long distance PCR - (reply: 39)
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TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
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Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
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TOUCH DOWN PCR - (reply: 2)
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Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
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PCR stopped working - After changing buffer (reply: 5)
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ChIP PCR question - (reply: 33)
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PCR protocol questions! - (reply: 2)
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TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
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DNA amount calculation for PCR - (reply: 14)
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ChIP primer design - (reply: 1)
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Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
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Bisulfite sequencing primer design query (probably simple) - (reply: 4)
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Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
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Questions regarding RT-PCR optimization - (reply: 2)
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number of copies after pcr? - (reply: 1)
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PCR double-bands - (reply: 1)
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Problems with semi-quatitative PCR - (reply: 1)
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Question about Primers - (reply: 3)
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Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
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Large fragment amplification failed - (reply: 3)
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purification of PCR product for cloning in a vector - (reply: 3)
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elimination of Protein interference from DNA - for RT-PCR (reply: 2)
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PCR reaction calculation - (reply: 2)
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designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
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Primer design with a tag - (reply: 4)
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no increase in fluorescence in my real time PCR - (reply: 3)
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PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
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Help with real-time PCR quantification of miRNA - (reply: 8)
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PCR, using gel extracted band as a template - (reply: 4)
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PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
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Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
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PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
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Combining forward and reverse primer gives different size on gel - (reply: 2)
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Q-PCR and RT-PCR - (reply: 3)
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calculating total amount - in a pcr reaction (reply: 4)
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Problems with amplifying microRNA - (reply: 3)
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PCR of complement genomic DNA - (reply: 3)
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primers for fungal identification - (reply: 9)
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confusing PCR - (reply: 1)
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Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
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PCR for long and repetitive region from genomic DNA - (reply: 4)
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difference between PCR primers and sequencing primers - (reply: 1)
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How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
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PCR of repeated region - (reply: 1)
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Sense and antisense DNA, and primer design - (reply: 6)
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PCR bands in NTC but NOT in negative samples - (reply: 2)
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housekeeping genes in PCR - (reply: 1)
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Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
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Question about RealTime PCR Primer Design - (reply: 1)
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Designing a Primer with a RS that has a W - (reply: 1)
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Primers stopped working!? - PCR Primers (reply: 6)
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NFK beta primers - (reply: 4)
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final PCR product - need clarifications (reply: 1)
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Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
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Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
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Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
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RT-PCR result !! - (reply: 4)
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mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
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Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
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Agarose gel electrophoreses for PCR products ? - (reply: 4)
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why genomic can't be use for pcr? - (reply: 2)
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Primer design - Primer design (reply: 2)
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Re-use unamplified PCR product - (reply: 2)
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Reason for odd PCR conditions - (reply: 3)
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Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
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Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
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pcr - problem in pcr (reply: 2)
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Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
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How to present and analyze these real-time PCR data? - (reply: 2)
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Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
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RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
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PCR Master mix - (reply: 2)
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cDNA storage and real time RT PCR - (reply: 3)
-
Real Time PCR Primers and Probes - (reply: 2)
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Anyone make their own PCR cloning vector? - (reply: 5)
-
Stability of PCR T-overhangs - (reply: 1)
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Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
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PCR product wrong size! Need help! - (reply: 9)
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pooling different pcr cycles? - (reply: 1)
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Amplification of concatenated linear ligated fragments - (reply: 4)
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Clinical Real-time PCR assay - (reply: 1)
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Need help - How we can design primer for miRNA (reply: 1)
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primer and promoter sequences - (reply: 1)
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Standard curve for real-time PCR - (reply: 2)
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designing of primers - (reply: 2)
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Primer Tm differences/ PCR - (reply: 1)
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Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
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single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
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d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
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BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
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Designing primer to remove his-tag - (reply: 1)
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PCR Primer Dilution from F+R - (reply: 1)
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primer reconstitution - primer reconstitution (reply: 4)
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Not-Quite-Nested PCR - (reply: 2)
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PCR product too short - (reply: 6)
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Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
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Primer design for insert amplification - Is this correct? (reply: 2)
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got smear on the PCR gel !!! - (reply: 4)
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need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
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"Unzipped" PCR band - (reply: 3)
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Review on new amplification techniques - (reply: 2)
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What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
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DOT BLOT for pcr product - No result obtain (reply: 1)
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melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
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Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
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HRM primers - (reply: 1)
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PCR amplification with Pfu / quality of DNA - (reply: 4)
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primer design - (reply: 2)
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Is it possible to compare data between real-time PCR plates - (reply: 1)
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Conventional PCR problems - wisamni2004 (reply: 2)
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New to sequencing, primer design - (reply: 2)
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Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
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Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
-
incorrect/smaller band size for real time PCR - (reply: 1)
-
Quickchange mutagenesis primer - (reply: 3)
-
pcr / primer theory in bisulfite sequencing - (reply: 4)
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homologous amplicon for real time pcr - (reply: 1)
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Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
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How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
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assistance with PCR amplification please - (reply: 3)
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I need an urgent help with q-pcr amplification plots - (reply: 11)
-
Problems with the Specificity of the Primers - (reply: 2)
-
confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
-
Colony PCR with FALSE Positive Results ?? - (reply: 4)
-
Degenerate primers PCR problem, Please help! - (reply: 2)
-
Hotstart PCR and unspecific Amplification - (reply: 3)
-
Primer design help needed - (reply: 1)
-
Primers no longer work - (reply: 1)
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Sequencing - why are left-over PCR primers an issue? - (reply: 3)
-
Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
-
RT PCR for known snps detection? - SNPs detection (reply: 1)
-
colony PCR - (reply: 2)
-
Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
-
primer Tm is too high, how tu get pcr product - help! (reply: 21)
-
PCR reaction without template gives a product - (reply: 6)
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PCR wrong product size - (reply: 6)
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Wrong pcr product size - (reply: 4)
-
real time PCR machine - (reply: 3)
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PCR very faint product - (reply: 1)
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Question about principle of PCR - (reply: 3)
-
PCR amplification before bisulfite conversion - (reply: 6)
-
4 question for real time PCR - (reply: 5)
-
Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
-
PCR didn't work - suspect the enzyme (reply: 2)
-
Find universal primers in vector - any online tool? (reply: 6)
-
Picking primers to confirm Illumina meth27 results - (reply: 2)
-
Colony PCR Specificity - (reply: 2)
-
Colony Pcr - primers - (reply: 5)
-
iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
-
Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
-
Primer Dilution Problem - D'oh! (reply: 2)
-
Using PCR to create overhangs - (reply: 3)
-
Creating overhangs with PCR - (reply: 4)
-
Problem with PCR on bacmids - (reply: 1)
-
Is there any software that can be sued to design degenerate primers - (reply: 1)
-
Running real time pcr product on gel?.. - (reply: 4)
-
Primer Decontamination - (reply: 2)
-
PCR mutagenesis of plasmid - (reply: 5)
-
tools for checking non-specific binding of primers -
(reply: 1) -
Primer designed for ARMS PCR - (reply: 2)
-
Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
-
methylation specific PCR - Primers - (reply: 1)
-
bacterial identification using real time PCR - (reply: 1)
-
Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
-
real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
-
PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
-
colony PCR for subcloning gene - (reply: 1)
-
Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
-
Using the same PCR plate in more runs - Just curious about it. (reply: 1)
-
PCR reamplification - (reply: 2)
-
Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
-
How many colonies to screen (colony PCR)? - (reply: 4)
-
Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
-
Primer Design - aligning sequences for probe/primer design (reply: 2)
-
question about pcr - (reply: 3)
-
RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
-
How to construct a standard curve for real time PCR - (reply: 1)
-
Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
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PCR with one primer - (reply: 1)
-
colony PCR inconsistent - (reply: 4)
-
PCR contamination - (reply: 19)
-
PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
-
If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
-
can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
-
PCR not working - No amplification - (reply: 5)
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Quantification of PCR products - (reply: 1)
-
fermentas clonejet pcr cloning kit - want opinion (reply: 1)
-
Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
-
qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
-
Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
-
Nested PCR question - (reply: 1)
-
High Tm on primers - cannot get a product - (reply: 8)
-
Help: Very odd amplification graphs (RTPCR) - (reply: 3)
-
undesired products in multiplex PCR - (reply: 3)
-
NCBI primer blast problem - (reply: 1)
-
Help with Multiplex Nested PCR - (reply: 2)
-
Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
-
Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
-
primer sequence problem - (reply: 2)
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Finding bisulfite PCR primer sequence - (reply: 3)
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PCR -No band formation - (reply: 1)
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PCR - No Band formation - (reply: 3)
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No amplification after bisulfite treatment - (reply: 4)
-
PCR AMPLIFICATION - (reply: 1)
-
Ligation of Blunt PCR Product - (reply: 1)
-
real time pcr need advise - (reply: 2)
-
Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
-
PCR & Cloning - (reply: 6)
-
Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
-
use of housekeeping gene in RT PCR chIP - (reply: 3)
-
DNA pooling for PCR - Saving money PCR (reply: 7)
-
Real Time PCR Standard curves - How many are required??? (reply: 1)
-
PCR need some help - (reply: 4)
-
How to design primers to check my candidates in a ChiP assay - (reply: 4)
-
Left polymerase out overnight - Will it still work? (reply: 1)
-
Primer Reconstitution--does temperature matter? - (reply: 5)
-
Primer Design MSP, BSP, MS-HRM - (reply: 3)
-
Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
-
PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
-
Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
-
Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
-
no PCR product from input... - (reply: 8)
-
MSP - unwanted amplification - (reply: 5)
-
Smears in Bisulfite seq. PCR - (reply: 3)
-
Primer design for qPCR - (reply: 2)
-
Primer design for qPCR - (reply: 1)
-
DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
-
Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
-
Multiple bands after bisulfite PCR - (reply: 7)
-
PCR genomic DNA of high GC content - (reply: 6)
-
GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
-
primer design tips - (reply: 3)
-
PCR with Biotin Incorporation - (reply: 2)
-
pcr product - hello all (reply: 1)
-
design pcr primer - (reply: 1)
-
promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
-
amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
-
PCR with long and complex primers - (reply: 2)
-
Phospholylation of primers - (reply: 1)
-
apoptosis by PCR? - (reply: 12)
-
Re-using PCR plates? - (reply: 2)
-
Touchdown PCR issues - (reply: 3)
-
LINE1 OFR2 Primer Design - (reply: 1)
-
phosphorylation of primers using PNK - (reply: 1)
-
RT-PCR contamination issue - (reply: 3)
-
Primers for yeast (18S) - (reply: 1)
-
How to reduce the bisulfite PCR bias? - (reply: 4)
-
What's the longest overhanging primer seq. you have used? - (reply: 2)
-
Primer Concentration to lower Ct - (reply: 1)
-
Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
-
Primer Concentration Help - (reply: 4)
-
who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
-
Primer Degradation - What exactly happens? (reply: 1)
-
Smearing on ategarose gel of real time pcr product??? - (reply: 1)
-
PCR/DNA Extraction Problem - (reply: 5)
-
Primers in 2 exons - (reply: 2)
-
NTC appear in real time pcr - (reply: 6)
-
Problems with designing a primer - (reply: 2)
-
How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
-
PCR of AT-rich DNA - (reply: 1)
-
T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
-
Overlapping PCR - really need help ! - (reply: 5)
-
Freezing PCR product??? - (reply: 1)
-
problem with pcr cloning from mouse cDNA - (reply: 2)
-
Probe or Primer info for bovine AQP-1 - (reply: 6)
-
PCR with Platinum Taq - product yield issues - (reply: 3)
-
real time PCR - (reply: 7)
-
Different sized product for same BS primer! - (reply: 2)
-
strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
-
How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
-
PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
-
Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
-
Differences between primers for real-time PCR and RT-PCR - (reply: 6)
-
primers with restriction site - (reply: 1)
-
PCR product appear two close band in my gel - (reply: 15)
-
The Case of a Missing Band: PCR Issue - (reply: 4)
-
PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
-
cDNA as PCR template - (reply: 1)
-
degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
-
cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
-
paranormal qpcr amplification activity - melting curves (reply: 3)
-
Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
-
PCR template concentration - (reply: 6)
-
Bands in PCR negative control - (reply: 3)
-
PCR efficiency calculated by Linreg - (reply: 3)
-
Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
-
primer design by generunner DG - (reply: 1)
-
degenerate pcr - (reply: 1)
-
BSP primers design, help please - (reply: 2)
-
primers with fluoresent dye PCR cycle question - (reply: 4)
-
Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
-
Smears on PCR products - (reply: 4)
-
Dumb Reverse Transcription PCR Question - (reply: 2)
-
Reverse transcription PCR - (reply: 1)
-
PCR/RT-PCR beads - (reply: 2)
-
High Amplification Efficiency in Std. Curve - (reply: 5)
-
Non specific products in Bisulphite pcr - (reply: 4)
-
Run PCR amplifications in agarose gels - (reply: 3)
-
Problems with validating primers and low expression genes - (reply: 3)
-
Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
-
Bisulfite sequencing PCR not working - (reply: 5)
-
[Help]universal tag - universal tag in multiplex PCR (reply: 4)
-
Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
-
intron/exon spanning primers - (reply: 1)
-
can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
-
expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
-
PCR product for sequencing - sample storage?? (reply: 2)
-
Absolute quantification using Real Time PCR - (reply: 2)
-
PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
-
question about blunt ligating Taq-amplified PCR product - (reply: 2)
-
RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
-
MSP primer troubles - (reply: 3)
-
Measuring global methylation using real time PCR - (reply: 2)
-
Designing Primers for multiple Isoforms - (reply: 1)
-
RT-PCR standard curve dilutions - (reply: 2)
-
Rt-PCR problem !with 2.5 kb gene - (reply: 3)
-
real time pcr melt curve and primer efficiency problem - (reply: 3)
-
Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
-
how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
-
PCR product dimer issue - (reply: 24)
-
PCR additives Formamide - Formamide which one? (reply: 4)
-
PCR Master mix - (reply: 1)
-
Addition of Restriction sites into PCR primers - (reply: 4)
-
Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
-
failure PCR amplification from low GC content gene - (reply: 5)
-
How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
-
conventional v real-time PCR applications - (reply: 1)
-
Primer Design in 3´non translated version vs. coding region - (reply: 2)
-
PCR reagents, can I use reagents from different manufacturers? - (reply: 4)
-
different PCR primers for real-time and classic PCR - (reply: 3)
-
primers deconamination??? - (reply: 6)
-
PCR band too thick - PCR troubleshooting question (reply: 5)
-
RT-PCR problem - (reply: 1)
-
Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
-
primer check!!! - (reply: 6)
-
PCR bands on gel electrophoresis - (reply: 5)
-
Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
-
long range PCR - (reply: 2)
-
Annealing Temperature of biotinylated primers - (reply: 2)
-
RT-PCR - New to RT-PCR info (reply: 2)
-
PCR punch - (reply: 4)
-
good amplification in classic PCR, no amplification in qPCR - (reply: 6)
-
PCR off plasmid for screening - (reply: 2)
-
PCR failed no product.. help - (reply: 10)
-
number of molecules in PCR - (reply: 3)
-
Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
-
primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
-
Primer clean-up - ExoSAP-It isn't working (reply: 5)
-
Primer dilution - PCR primer dilution (reply: 2)
-
Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
-
Can't re-PCR the PCR product - (reply: 5)
-
PCR reagent autoclave - (reply: 2)
-
Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
-
Long Primers for PCR - (reply: 2)
-
Data Analysis for Real-time PCR - (reply: 2)
-
PCR not working - overhangs too long? (reply: 3)
-
Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
-
argh more pcr headaches! - (reply: 6)
-
With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
-
Chlamydia PCR cycling conditions - (reply: 1)
-
Primers going "loopy" - (reply: 2)
-
Conversion of primer unit - ug/ul to uM (reply: 4)
-
mutagenesis by PCR or just use a kit - (reply: 5)
-
Unsuccessful BS PCR - (reply: 3)
-
Primer Trouble Shooting - (reply: 5)
-
Proven Primers Stopped working & now leave smear! - (reply: 2)
-
Smearing on gel in PCR products - (reply: 5)
-
Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
-
Relative RT-PCR, multiplate and calibration - (reply: 4)
-
Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
-
Relative RT-PCR - (reply: 2)
-
Relative real time RT PCR - (reply: 2)
-
DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
-
40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
-
a simple primer problem - (reply: 5)
-
Failure to introduce mutations using Overlap PCR - (reply: 3)
-
no or poor amplification - (reply: 1)
-
Primers from 3'UTR - (reply: 2)
-
Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
-
problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
-
Designing primers with ESTs - (reply: 2)
-
Direct sequencing of Bisulphite PCR product - (reply: 1)
-
To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
-
Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
-
Real Time vs Traditional PCR results - (reply: 1)
-
The problems to identify mouse genotype with PCR - (reply: 1)
-
addition of BclI restriction site to PCR primer - (reply: 6)
-
non specific amplification - DNA amplification by PCR (reply: 1)
-
Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
-
How does methprimer calculate primer Tm - (reply: 2)
-
Laminar Flow vs PCR cabinet - (reply: 4)
-
How to do PCR to detect mRNA without RT? - (reply: 2)
-
first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
-
Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
-
PCR has stopped working - (reply: 7)
-
PCR negative control contamination - (reply: 8)
-
LacZ PCR genotyping -ve control contamination - (reply: 3)
-
Phosphorylated primers - (reply: 5)
-
screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
-
PCR efficiency in real timePCR - (reply: 5)
-
Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
-
Drastic Decrease in PCR product yield - (reply: 2)
-
resources on PCR principles & technique - (reply: 1)
-
pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
-
Colony Screeing without using PCR - (reply: 8)
-
High MW PCR band seen...help needed - (reply: 7)
-
QPCR - Primer and Probe question - QPCR (reply: 2)
-
Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
-
standard curve/ PCR efficiency problems... - (reply: 1)
-
Help! PCR that used to work doesn't work now! - (reply: 2)
-
PCR and templates - (reply: 3)
-
does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
-
Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
-
PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
-
Easy primer question? - Primers (reply: 1)
-
PCR inconsistency - (reply: 4)
-
Plasmid problem - From PCR product (reply: 7)
-
help needed: PCR a gene from genomic DNA - (reply: 2)
-
questions about pcr products after pooling - (reply: 3)
-
stiching/linking/sewing pcr - (reply: 3)
-
Human mtDNA amplification problems - (reply: 3)
-
primer dimer - proplems (reply: 1)
-
Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
-
Nested PCR - (reply: 2)
-
pfu vs long pcr mix - (reply: 7)
-
weird PCR ask for help - (reply: 12)
-
Methylation specific DMSO PCR - (reply: 1)
-
The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
-
No PCR product at all - (reply: 8)
-
PCR troubleshoooooting - primer dimer (reply: 1)
-
Primer efficiency test - (reply: 1)
-
primer software - (reply: 2)
-
Question on wired PCR - (reply: 1)
-
restriction digestion of PCR product - (reply: 7)
-
Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
-
Checking PCR insert (into pGEMT vector) - (reply: 2)
-
How to know in which exon a primer match? - (reply: 1)
-
primer annealing - higher annealing= less wrong bounds (reply: 4)
-
Amplification of human genomic DNA - (reply: 2)
-
trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
-
sequencing with forward/reverse primers - (reply: 7)
-
How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
-
PCR troubleshooting - two band after agrose electophoresis (reply: 4)
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Adding A overhangs - primer design implications? - (reply: 1)
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primer RE over-hang nucleotides - common sequences? (reply: 5)
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Forward and reverse primers got very different Tm - what to do? (reply: 11)
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Colony PCR screen positive - insert digestion negative - (reply: 2)
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Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
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Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
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primer design problem - (reply: 1)
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Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
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Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
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Problem with primer efficiency analysis - (reply: 4)
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slan real time PCR system for validation of microarray results? - (reply: 1)
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PCR unusualband at ~230bps - PCR (reply: 1)
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DMSO or BSA for PCR - (reply: 6)
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Whole Genome Amplification - (reply: 2)
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primer design - (reply: 1)
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Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
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Separating PCR product on agarose gel with similar sizes - (reply: 5)
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designing primers for genes not sequenced yet - (reply: 2)
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Tris Buffer for PCR reaction - why? (reply: 1)
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PCR a plasmid protein - (reply: 2)
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multiplex PCR - (reply: 4)
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Realtime PCR machine - (reply: 2)
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PCR set-up calculation nightmares. - (reply: 3)
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Primer desing - (reply: 2)
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Colony PCR - (reply: 3)
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Standard curves for PCR efficiency. - (reply: 2)
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Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
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Question about RAPD PCR - (reply: 2)
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Looking for help with my PCR! - (reply: 3)
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How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
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Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
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primer design - (reply: 9)
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problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
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PCR without thermal cycler? - (reply: 9)
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primer contamination - primer contaminated with ice (reply: 3)
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Loss of volume in PCR reaction in 96 well plate - (reply: 7)
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RT-PCR problem - (reply: 4)
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Only DNA ladder , No desired band in PCR - (reply: 4)
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taq and PCR - (reply: 6)
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Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
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PCR Efficiency - (reply: 2)
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DNA and RNA contamination in RT PCR water controls - (reply: 2)
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Long PCR and genomic DNA isolation problems - (reply: 2)
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Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
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NTC with specific amplification - (reply: 1)
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Problem cloning bisulfite PCR BSP product - (reply: 6)
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Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
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Primer3 vs Primer BLAST - (reply: 3)
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PCR and then ligation - (reply: 7)
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Primer design: free energy - (reply: 2)
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Amplification in NTC and noRT controls - (reply: 5)
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realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
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Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
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asymmetric PCR - (reply: 1)
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PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
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CORRECT PCR Incorrect RTPCR - (reply: 10)
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Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
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trouble with pcr - (reply: 8)
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Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
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Confusing bands from PCR - not primer dimer, not product (reply: 30)
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Real time PCR results-interpretation - (reply: 1)
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Real time PCR results-interpretation - (reply: 2)
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Primer optimization for ChIP - (reply: 2)
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sequencing primer: T7 or T7promoter? - (reply: 4)
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2-step or 3-step real time PCR - question about real time PCR (reply: 6)
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Problems with my PCR's - Having an issue with some of my samples (reply: 2)
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SYBR melting curve vs RT-PCR gel - (reply: 1)
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primer tm - tm calculation for tagged primers (reply: 2)
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Reusing 96 well plates for PCR - (reply: 2)
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Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
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PCR and AFLP - (reply: 1)
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Universal 16s rRNA primers needed - (reply: 3)
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Identifying PCR Inhibitors - (reply: 10)
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PCR primers for histone mod ChIP - How to find the regions (reply: 1)
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PCR + phusion enzyme = massive errors - (reply: 4)
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long DNA amplification - (reply: 11)
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same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
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gDNA pcr product as standard for absolute quantification? - (reply: 1)
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adding koazak sequence to primer - (reply: 1)
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PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
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Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
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pcr trouble solved but I cant seem to understand why - (reply: 1)
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Primer design if sequence is unknown for organism - (reply: 5)
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adding C terminal tag to reverse primer - (reply: 1)
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epitope tagging of pcr products - (reply: 1)
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Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
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Real time PCR info - (reply: 4)
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Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
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PCR from BAC - PCR from BAC (reply: 1)
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Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
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Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
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E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
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How much product PCR?? - (reply: 3)
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what primers should I use for DNA sequencing? please help - (reply: 2)
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what primers should I use for DNA sequencing?? - (reply: 3)
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Is my primer going to work for qRT-PCR? - (reply: 5)
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Standard curve for RT-PCR - (reply: 1)
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PCR using genomic DNA - (reply: 2)
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pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
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Problem amplifying plasmid - (reply: 1)
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Sybr Green I in real-time PCR reaction - (reply: 3)
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Primers annealing temperatures - (reply: 8)
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Difficulties cloning by PCR - (reply: 5)
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PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
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Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
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high Cp value PCR - (reply: 2)
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real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
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Synthesize own Oligo-dT primers - (reply: 6)
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Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
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shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
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dNTP or Deoxynucleotide solution Mix - (reply: 1)
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Smearing/ no amplification in PCR - (reply: 3)
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primer design - Primer design for PCR (reply: 1)
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How are primers designed? - (reply: 4)
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Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
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DNA methylated PCR - (reply: 2)
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need help on primer calcuation soon - (reply: 1)
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primer design - (reply: 6)
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problem in pcr and purification - (reply: 3)
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Inability to duplicate PCR results - (reply: 8)
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Direct PCR (PCR without DNA extraction) - (reply: 2)
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PCR using xt5 - (reply: 2)
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long range PCR - purification of pcr frogament 9-13kb (reply: 9)
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BSP PCR for help - no bands of tissue amplification (reply: 3)
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Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
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2 bands or smear? 2-step RT-PCR - (reply: 1)
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23S rRNA Primer - need primer sequence (reply: 1)
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Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
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RT-PCR - problems with TaqMan PCR (reply: 2)
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Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
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Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
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template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
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Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
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Small product amplification problems - (reply: 4)
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multi way ligation or long range pcr? - (reply: 1)
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Freezing primers+SYBR green - (reply: 2)
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Tagged Primers - (reply: 5)
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no pcr amplification product - (reply: 9)
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Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
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design primer for chip - (reply: 5)
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How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
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PCR problem - (reply: 4)
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differently behavioring replicates in real-time PCR - (reply: 1)
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Multiplex PCR - (reply: 1)
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Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
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No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
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Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
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Volume of DNA to add to PCR Master Mix? - (reply: 1)
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how much DNA you need for real time pcr after chip - CHIP (reply: 1)
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PCR components storage - (reply: 3)
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reconstitution of primers - (reply: 2)
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No PCR bands with some templates - (reply: 5)
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Mysterious PCR Contamination - (reply: 13)
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BS PCR..please help - DNA methylation analysis (reply: 15)
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RT-PCR Help - Protocol provided (reply: 4)
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PCR product purification - (reply: 3)
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Mutagenesis PCR and undesired amplification - (reply: 2)
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Temp Gradient PCR - (reply: 2)
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Vector sequence included in (PCR) amplified insert - (reply: 2)
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PCR with genomic dna - (reply: 9)
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primer dimer - (reply: 2)
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PCR with long primers - (reply: 3)
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BSP PCR standardization...help - (reply: 8)
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PCR on degraded templates - (reply: 2)
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PCR help needed - (reply: 3)
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want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
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Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
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light bands, dark smear, dark dimers - (reply: 2)
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PCR, followed by sequencing... - why ?? (reply: 3)
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PCR program - strange extension step (reply: 2)
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Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
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Help me! How to design nested BSP primers? - (reply: 4)
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REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
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Plasmid linearization by PCR - (reply: 4)
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In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
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Using BLAST to check primers - (reply: 17)
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ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
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pcr doesnt show any band for DNa walking - (reply: 8)
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storing large amounts of PCR mastermix beforehand - question (reply: 3)
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questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
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Effects of more cDNA in PCR??? - (reply: 1)
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PCR primer - (reply: 2)
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PCR product disappears after restriction digestion - (reply: 4)
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Primer design - GC clamp - (reply: 8)
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Real Time PCR - excluding Ct - (reply: 2)
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PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
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Price of a real time PCR machine? - (reply: 4)
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colony PCR - (reply: 8)
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probe conc. of Asymmetric PCR - (reply: 1)
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primer dimer or what? - (reply: 2)
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PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
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Real Time PCR method comments - (reply: 2)
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Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
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colony pcr - (reply: 4)
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Primers and Taqman Probes mixture question - (reply: 1)
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truble shooting qiagen 1 step rt pcr kit - (reply: 2)
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ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
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Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
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Colony PCR - (reply: 15)
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Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
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no bands in my PCR - (reply: 9)
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designing primers - (reply: 1)
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Real Time PCR issues - Problems regarding my qPCR (reply: 6)
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purefication and amplification of serum free DNA - (reply: 1)
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How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
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Bisulfite Sequencing PCR - (reply: 4)
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Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
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way to know if my UNOII pcr machine is working properlly - (reply: 3)
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Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
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mRNA PCR - (reply: 1)
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pcr product quantification - (reply: 5)
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Negative Control Primers for ChIP Assay - (reply: 3)
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Magnesium in PCR - (reply: 1)
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PCR Sample Prep - (reply: 4)
-
Bisulfite PCR Inconsistent, often smears - (reply: 4)
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PCR from roots - I know this sounds a little crazy... (reply: 2)
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Bizarre Contamination in PCR - (reply: 7)
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PCR Cloning-large primers - (reply: 4)
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Quantifying ingested bacteria without realtime PCR - (reply: 3)
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forward and reverse primer - (reply: 4)
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Check the primers - (reply: 4)
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PCR problem from transformed TOPO TA vector - (reply: 3)
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DIG DNA PCR labeling problem - (reply: 6)
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PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
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BSP primers - (reply: 2)
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Various size of insert after colony screen by PCR - (reply: 2)
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inverse PCR molecular bilogy - (reply: 3)
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PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
-
failed PCR on DNA extract from blood - (reply: 6)
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Will ligase buffer affect polymerase fidelity? - (reply: 2)
-
Primer dimer issue in real time PCR - (reply: 20)
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Leaky RT-PCR - (reply: 4)
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Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
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PCR, RNA, Northern Blotting???? - (reply: 1)
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bizzare PCR smear - why does science hate me (reply: 7)
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RT-PCR primer dimers and cDNA degradation - (reply: 2)
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PCR detection of SNP - (reply: 6)
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PCR efficiency important in real time absolute quantification? - (reply: 4)
-
PCR contamination with human DNA first and now no bands!!! - (reply: 4)
-
methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
-
Sub-cloning sticky-end PCR products - (reply: 2)
-
sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
-
ChIP on chip amplification problems - (reply: 10)
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Genotyping PCR - (reply: 3)
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PCR screening of transformed bacterial colony - (reply: 6)
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Is this standard valid? - pcr product as standards (reply: 5)
-
primer binding - (reply: 1)
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weird ChIP primers! - (reply: 1)
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PCR primers - (reply: 1)
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Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
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Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
-
amplification in negative control in RT PCR - (reply: 2)
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miRNA real time PCR by SYBR green methods - (reply: 1)
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Deletion PCR - (reply: 9)
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diluting to final primer concentration - please help correct (reply: 1)
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Smear problem with my new primer set - (reply: 2)
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PCR ghost bands - (reply: 1)
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Amplifying a gene using a degenerate primer - (reply: 4)
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DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
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CP of real time PCR by LC480 - (reply: 1)
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CHIP- Real Time PCR calculations - (reply: 1)
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volume of DNA required (PCr product ) in agarose gel - (reply: 11)
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transgene copy number - using real time PCR (reply: 1)
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real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
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Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
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Unspecific PCR - (reply: 7)
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Real-Time PCR as a Microplate Reader - (reply: 1)
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gene-specific RT-PCR - (reply: 4)
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Primer Design - Design Primer with DNA Sequence (reply: 1)
-
RT-PCR Internal Standard - (reply: 2)
-
PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
-
Plasmid supercoiling affecting PCR? - (reply: 2)
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RNA contamination in PCR - (reply: 1)
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PCR without DNA extraction! - (reply: 2)
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Primer tm - (reply: 1)
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How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
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re-amplification in real-time PCR - (reply: 1)
-
primer design - really necessary? (reply: 2)
-
TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
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RT-PCR - (reply: 3)
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Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
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Degenerative primers = multiple products? - (reply: 5)
-
Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
-
PCR problems on a ligation product - (reply: 5)
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Genomic DNA and PCR - (reply: 4)
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Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
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Sewing PCR - (reply: 3)
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on the minimal pcr derived product - (reply: 2)
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primer dimers - (reply: 5)
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RT-PCR vs. conventional PCR - (reply: 1)
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qPCR primer design - (reply: 5)
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PCR and gel purification problem - did I screw this up (reply: 4)
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Cloning PCR fragment - (reply: 1)
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touchdown pcr - (reply: 6)
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non specific pcr products - (reply: 4)
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RT-PCR primer design - Intron/exon boundaries - (reply: 6)
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Anyone with experiences in 2 step PCR - (reply: 1)
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PCR works on lab strain but not patient sample - HIV related (reply: 4)
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confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
-
DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
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Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
-
re-amplification of a PCR product - is it recommended? (reply: 7)
-
mouse Gapdh chip-qPCR primers for control! - (reply: 7)
-
PCR machine with accurate temperature control? - (reply: 1)
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Gene-specific RT-PCR - (reply: 2)
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Primer stock confusion - (reply: 6)
-
VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
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primer design - primer design question (reply: 2)
-
Protocol for 16S rDNA real time PCR - (reply: 2)
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Touchdown PCR - (reply: 1)
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Determine annealing temperature of primers - (reply: 14)
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Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
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polymerase for BSP - (reply: 2)
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real time PCR primer designing problems,again - is NCBI's primer blast believable enough？ (reply: 3)
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Validating real time pcr primers - (reply: 2)
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BSP,MSP primer design? - (reply: 1)
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low gc primers - help with pcr using low gc primers (reply: 6)
-
Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
-
Primers and annealing temperature - (reply: 2)
-
Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
-
Copy number calculations in real time PCR - (reply: 2)
-
4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
-
Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
-
RT-PCR Negative controls - (reply: 1)
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WHich primer to use for sequencing - (reply: 5)
-
New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
-
minimum length for the gene to be amplified in PCR - (reply: 6)
-
dNTP Quantity - (reply: 3)
-
annoying PCR cloning problem (season 2) - (reply: 5)
-
PCR with 60 bp primers - results in no product (reply: 6)
-
Amplification dwindles using little template RT-PCR - (reply: 3)
-
Running gel after RT-PCR - (reply: 5)
-
Degenerate PCR Size Limit? - (reply: 3)
-
primer design for pBAD topo expression kit - (reply: 2)
-
PCR problems.. - problems with conventional PCR (reply: 3)
-
18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
-
gDNA or cDNA amplification in RT-PCR - (reply: 3)
-
small PCR products on agarose gel? - (reply: 8)
-
Dnase digestion and PCR - (reply: 4)
-
Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
-
primers fpr sequencing - (reply: 4)
-
MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
-
Need an UV260 RT-PCR instrument - (reply: 1)
-
Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
-
Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
-
PCR produces products of wrong size - (reply: 4)
-
PCR with pfu and degenerate primers (?) - (reply: 7)
-
a doubt about PCR gel purification - (reply: 4)
-
Draw PCR primer locations - PCR primer (reply: 4)
-
how to design PCR site-directed multiple mutagenesis - (reply: 1)
-
RT-PCR primers on coding sequence or UTRs? - (reply: 4)
-
Program for mapping primers to gene - diagram maker, visualize - (reply: 3)
-
Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
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PCR+enhancer - (reply: 4)
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Serious issues with RT-PCR - (reply: 7)
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PCR [dNTP] - dNTP concentrations (reply: 2)
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PCR issue - (reply: 2)
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dNTP question - (reply: 3)
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PCR - (reply: 1)
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long PCR primer - (reply: 7)
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Taqman rtPCR primer and probe design - (reply: 3)
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sequencing problem after pcr - (reply: 4)
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Amplifying plasmid - non specific binding of primer - (reply: 3)
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Help with Real Time PCR Well To Well Variation - (reply: 10)
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primer design - (reply: 3)
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Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
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Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
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Analysis of ChIP RT-PCR data - (reply: 7)
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Make construct so it is only thing that can PCR after transfection - (reply: 2)
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Negative flouroscence in real time PCR - (reply: 2)
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Minimum number of PCR cycles to see a product? - (reply: 6)
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decontamination - decontamination for PCR (reply: 6)
-
Troubleshoot ARMS PCR - (reply: 2)
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negative control for PCR - (reply: 1)
-
how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
-
design primer - when I design a primer is it necessary to include the restriction (reply: 1)
-
pcr pdt amplification - (reply: 1)
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real time PCR - (reply: 3)
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PCR fails when I scale up - (reply: 2)
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can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
-
PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
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PCR not Working = SMEAR - (reply: 3)
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How to do PCR fragment in 2 steps - (reply: 3)
-
Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
-
where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
-
Real-Time RT-PCR one-step and two step issue - (reply: 12)
-
RT-PCR - problem of PCR (reply: 1)
-
PCR product longer than template - why? - (reply: 2)
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Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
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Dye recipe that can be used for PCR MM - (reply: 3)
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Primers Not working - Real time PCR using SYBR Green (reply: 3)
-
PCR conditions with three primers - (reply: 1)
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Real-Time PCR primer vs conventional PCR primer - (reply: 5)
-
distance between probe and primers in Taqman method - how far should they be? (reply: 2)
-
Cloning a labeled PCR product - (reply: 1)
-
PCR and cloning - (reply: 14)
-
QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
-
PCR product - not suppose to be there (reply: 7)
-
using the meth primer - (reply: 2)
-
Generating primers for MSP - (reply: 1)
-
Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
-
real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
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PCR from a smear genomic DNA ? - (reply: 5)
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Primer design and need help - (reply: 11)
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Setting up a multiplex PCR assay. - (reply: 2)
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Programme for degenerate primer design - Programme for primer design (reply: 9)
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Negative Control for ChIP realtime PCR in Mouse - (reply: 11)
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cDNA and RT-PCR - (reply: 8)
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Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
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Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
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PCR problem - help me plsss (reply: 9)
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gene-specific qPCR primers for a multigene family - primer design (reply: 5)
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Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
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PCR efficiencies - (reply: 4)
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RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
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PCR quantification - (reply: 1)
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PCR quantification - (reply: 1)
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Long PCR product - (reply: 9)
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problem in Pcr amplification - (reply: 7)
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What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
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difference between hotstart and taq polymerase - (reply: 7)
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sequencing the pcr product - (reply: 15)
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Pcr amplification - primer designed from UTR regions (reply: 5)
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RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)