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PCR Related Discussions
  1. confirmation for primers dilution - (reply: 7)
  2. Problems regarding amplification of my gene - (reply: 3)
  3. PCR working, qPCR is *not*. - (reply: 6)
  4. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  5. Overlapping PCR, need help - (reply: 5)
  6. How does RT-PCR work? - (reply: 5)
  7. Denatured plasmid for pcr - (reply: 1)
  8. Possible primer dimer problem..Need solution!!! - (reply: 2)
  9. confusing with where to put primers and which ordination - (reply: 4)
  10. Help with primer concentration for sequencing - (reply: 1)
  11. PCR troubleshoot - (reply: 5)
  12. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  13. PCR Products not run well in the GEL - (reply: 1)
  14. PCR solution calculus - (reply: 4)
  15. Amplifying from pcr products - (reply: 7)
  16. Consistancy issue with PCR block - (reply: 1)
  17. Queries regarding PCR - (reply: 3)
  18. Design the primers - (reply: 10)
  19. Calculation of Taq Polymerase - (reply: 3)
  20. Best Taq for colony PCR? - (reply: 5)
  21. How two fragments joint together when doing fusion PCR? - (reply: 4)
  22. How to test my milleq water in q PCR about contaminants - (reply: 3)
  23. Mutagenesis PCR - (reply: 9)
  24. real time PCR primer design - (reply: 1)
  25. The PCR gods are frowning upon me - (reply: 9)
  26. PCR after T4 ligation? - (reply: 3)
  27. primers for cloning - (reply: 1)
  28. Degenerated Primers and their problems - (reply: 1)
  29. Sonication of small PCR amplicon - (reply: 1)
  30. primer design with restriction enzyme - (reply: 1)
  31. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  32. primer dilution help - (reply: 1)
  33. Testing primers on 'unknown' tissue - (reply: 1)
  34. How to remove inhibitory substances in PCR? - (reply: 2)
  35. need some advise about PCR of PKD1-human gene - (reply: 7)
  36. puzzled with PCR outcome after BS treatment - (reply: 2)
  37. nested PCR for low viral load- HBV patient sample - (reply: 2)
  38. troubleshooting stubborn PCR - (reply: 6)
  39. Measuring pcr fragments in a gel - (reply: 1)
  40. Amplification in HK gene but not for target gene - (reply: 3)
  41. could you help me with my stem loop RT primer? - (reply: 1)
  42. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  43. How long can I store at - 30°C my PCR mix? - (reply: 6)
  44. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  45. RT-PCR help - (reply: 2)
  46. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  47. PCR Temperature change control malfunctioning - (reply: 1)
  48. excess amount of primers - (reply: 2)
  49. Dehydrating and Reconstituting primers - (reply: 15)
  50. Trouble with PCR of short sequence - (reply: 3)
  51. Setting the temperature range for gradient PCR - (reply: 3)
  52. KIR gene promoters for MSP primers design - (reply: 2)
  53. Biotin-streptavidin signal amplification - (reply: 1)
  54. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  55. Amplification curve in the negative control samples - (reply: 7)
  56. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  57. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  58. Electrophoresis after PCR : too many bands - (reply: 6)
  59. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  60. Primer as limiting reagent in PCR reaction - (reply: 2)
  61. Primer Design for RNA probes - (reply: 2)
  62. Real time PCR for degraded RNA - (reply: 1)
  63. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  64. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  65. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  66. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  67. Nested BSP Primer Design - (reply: 6)
  68. website for primer design - (reply: 1)
  69. PCR Efficiency over 150%! - (reply: 1)
  70. problem of amplification - (reply: 2)
  71. PCR not working - (reply: 11)
  72. How to do a primer dilution - (reply: 10)
  73. Troubles with Fusion PCR - (reply: 1)
  74. Primer design and alternative transcripts - (reply: 2)
  75. PCR and sequencing of genomic DNA - (reply: 4)
  76. qPCR amplification - (reply: 4)
  77. NESTED PCR - (reply: 6)
  78. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  79. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  80. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  81. Digestion necessary after PCR? - (reply: 7)
  82. Inverse PCR product selection - (reply: 2)
  83. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  84. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  85. how to hasten real-time PCR amplifications - (reply: 2)
  86. Please please help me with my Phusion PCR. - (reply: 5)
  87. PCR - (reply: 1)
  88. NCBI Primer Design - Stringency Issues - (reply: 3)
  89. Different primer concentration in qPCR - (reply: 1)
  90. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  91. help in long pcr - (reply: 1)
  92. PCR inhibitor in template DNA - (reply: 3)
  93. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  94. Problem with Real-time PCR results analysis - (reply: 1)
  95. Primer Specificity: Testing only one primer - (reply: 4)
  96. PCR from protozoa DNA - (reply: 3)
  97. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
  98. tool for comparing many primers pairs - (reply: 4)
  99. PCR that leads to protein synthesis - (reply: 18)
  100. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  101. Use of DMSO in General PCR - (reply: 1)
  102. PCR product size confusion - (reply: 3)
  103. Pcr primers - (reply: 7)
  104. Concentration specification in PCR - (reply: 3)
  105. Guanidine isothiocyanate in PCR - (reply: 1)
  106. Primers have worked well but now getting primer dimers? - (reply: 2)
  107. I cannot design primers on exon-exon junction - (reply: 2)
  108. DNA Quantification of PCR Products - (reply: 2)
  109. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  110. Problem for PCR - (reply: 9)
  111. Designing primers in UTRs - (reply: 1)
  112. Multiplex PCR - (reply: 1)
  113. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  114. primer design@ buy? - (reply: 2)
  115. Trouble with overlap extension pcr - (reply: 3)
  116. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  117. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  118. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  119. Question about the RT PCR - (reply: 3)
  120. PCR ready mixes with long shelf lives - (reply: 4)
  121. Troubleshooting: Inverse PCR - (reply: 3)
  122. How to design primer to amplify genomic DNA? - (reply: 3)
  123. Overlap PCR, need help - (reply: 11)
  124. Internal control for miRNA RT-PCR - (reply: 1)
  125. Primers - (reply: 1)
  126. Primers mix - (reply: 2)
  127. question about RNA concentration for real time PCR - (reply: 1)
  128. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  129. PCR products sizes and DNA ladder - (reply: 7)
  130. Having problem with primers for qPCR - (reply: 4)
  131. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  132. PCR product sequencing - (reply: 3)
  133. Bad fragment amplification - (reply: 4)
  134. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  135. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  136. No amplification with TRAPEZE kit! - (reply: 1)
  137. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  138. Stargazer PCR problems - (reply: 3)
  139. General PCR discussion - (reply: 8)
  140. Real time. No amplification but hight flourescence - (reply: 1)
  141. Real time PCR doubt - (reply: 5)
  142. PCR Profile for ligation - (reply: 3)
  143. protocol to relieve melanin inhibition of PCR - (reply: 4)
  144. No bands despite different primers and conditions and TAqs - (reply: 8)
  145. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  146. Equimolar Mix Primer - (reply: 8)
  147. Wrong PCR product - (reply: 2)
  148. Colony PCR positive and Digestion negative????? - (reply: 11)
  149. Primer design - (reply: 5)
  150. storage for lyophilized primers - (reply: 1)
  151. PCR GAPDH gene - (reply: 1)
  152. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  153. Quantitative RT-PCR statistics help - (reply: 1)
  154. Dimerization of PCR product - (reply: 4)
  155. PCR Primer trouble - (reply: 2)
  156. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  157. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  158. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  159. finding the corrosponding primers - (reply: 2)
  160. Gene sequence for Real time PCR - (reply: 2)
  161. non-reproducible PCR results with cDNA as template - (reply: 1)
  162. Ligation of PCR fragments - (reply: 11)
  163. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  164. RNA extraction for RT-PCR - (reply: 3)
  165. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  166. problems with gDNA doing real time PCR in yeast - (reply: 2)
  167. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  168. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  169. How to get rid of bands in PCR negative control - (reply: 10)
  170. T7 and M13 primers two band amplification - (reply: 3)
  171. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  172. Strange PCR problem - (reply: 2)
  173. Question about Double Digestion followed by PCR amplification - (reply: 2)
  174. PCR problem - (reply: 2)
  175. PCR machine not working properly - (reply: 1)
  176. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  177. PCR gene specific amplification problem - (reply: 3)
  178. Failure SYBRGREEN PCR - (reply: 4)
  179. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  180. without RE site in PCR product - (reply: 5)
  181. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  182. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  183. real-time pcr non reproducible - (reply: 4)
  184. Strange amplification plots with high Ct variability - (reply: 1)
  185. How big a role does mixing play in PCR - (reply: 1)
  186. Melting curve is irregular for primer optimization - (reply: 5)
  187. Designing primers for ABO blood groups - (reply: 1)
  188. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  189. RT-PCR primer design - (reply: 7)
  190. How to amplify very short PCR template - (reply: 4)
  191. Is this primer okay? - (reply: 4)
  192. PCR amplification of large template - (reply: 1)
  193. PCR insert - in frame? - (reply: 2)
  194. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  195. smear in gel electrophoresis after PCR - (reply: 2)
  196. Whole plasmid amplification by PCR - (reply: 2)
  197. PCR product as standard curve template - (reply: 6)
  198. colony PCR after transformation - (reply: 1)
  199. PCR machine - (reply: 3)
  200. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  201. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  202. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  203. Resuspending primers calculation - (reply: 4)
  204. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  205. PCR RFLP is PCR product purification necessary? - (reply: 3)
  206. Mystery in my PCR - (reply: 5)
  207. Whole genome amplification from cDNA? - (reply: 4)
  208. What do you use to check primer secondary structure? - (reply: 3)
  209. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  210. PCR product as template for in vitro transcription - (reply: 1)
  211. Problem with 3 step PCR - (reply: 3)
  212. mutagenic primers with very high GC content. - (reply: 3)
  213. Scaling up PCR to get more DNA - (reply: 5)
  214. PCR to get 10kbp product - (reply: 4)
  215. site-directed mutagenesis primer Tm - (reply: 4)
  216. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  217. Random vs oligo primer in preamplification RT - (reply: 1)
  218. how does polymerase stop at the required length - (reply: 1)
  219. Chip pcr. are there inespecifics? - (reply: 1)
  220. Primer concentration - stupid question - (reply: 3)
  221. Primer Design, help i´m New - (reply: 4)
  222. No DNA after PCR product purification - (reply: 9)
  223. Sensitivity of RT-PCR and qPCR - (reply: 4)
  224. RT-qPCR primer problem - (reply: 4)
  225. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  226. real time PCR trouble - (reply: 3)
  227. removal of ethanol in PCR product purification - (reply: 5)
  228. Crazy real time PCR curve - (reply: 4)
  229. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  230. High fidelity PCR trouble shooting - (reply: 2)
  231. Designing cloning primers for DNMT - (reply: 2)
  232. Reproducible Non-Specific PCR Product - (reply: 2)
  233. Are these primer products good enough for qPCR? - (reply: 3)
  234. Can't get PCR with large overhang primers to work - (reply: 8)
  235. Primer Efficiency across runs - (reply: 1)
  236. Question about pipet tips for PCR and rtPCR - (reply: 4)
  237. UNG in PCR - (reply: 1)
  238. Cause of random samples failing PCR? - (reply: 2)
  239. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  240. pcr 10X buffer preparation - (reply: 2)
  241. how much template do i need for pcr? - (reply: 5)
  242. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  243. Problems with crossover PCR - (reply: 2)
  244. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  245. Designing PCR Primers for cloning - (reply: 17)
  246. Creating primers to add restriction sites to vector backbone - (reply: 7)
  247. PCR DNA Concentration - (reply: 1)
  248. RT-PCR product- no band - (reply: 4)
  249. Some primers dose not work - (reply: 5)
  250. Understanding RACE PCR - (reply: 1)
  251. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  252. Wierd Bands after PCR....Confused - (reply: 9)
  253. Query regarding primers for quick change mutagenesis - (reply: 3)
  254. PCR with Plasmid recovered from filter paper - (reply: 6)
  255. PCR amplified product size - (reply: 5)
  256. the storage time for primers - (reply: 9)
  257. Annealing temperature for PCR - (reply: 8)
  258. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  259. Nested PCR - (reply: 2)
  260. random primers or oligodT - (reply: 4)
  261. No amplification during RT-PCR - (reply: 4)
  262. Sequencing RT-PCR product - (reply: 3)
  263. Low yield PCR product after gel purification - (reply: 8)
  264. To design or use published primers? - (reply: 4)
  265. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  266. PCR primer usage (Clonning & cDNA) - (reply: 2)
  267. problem in cloning PCR primer design with restriction site - (reply: 4)
  268. Amplification with U primers, but not with M primers - (reply: 1)
  269. Taq polymerase - (reply: 7)
  270. His tag introduction in to gene and primer design - (reply: 2)
  271. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  272. Which High-Fidelity polymerase is better? - (reply: 2)
  273. polymerase to use for cloning - (reply: 4)
  274. Problem amplifying viral gene - (reply: 5)
  275. Adding tag using overlapping PCR - (reply: 2)
  276. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  277. Design primer from incomplete sequence... - (reply: 2)
  278. Design primer from incomplete sequence... - (reply: 2)
  279. How to perform colony PCR - (reply: 1)
  280. Primer designing for Methylation - (reply: 6)
  281. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  282. cDNA amplification problem - (reply: 4)
  283. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  284. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  285. PCR product running on agarose gel - (reply: 32)
  286. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  287. No bands in PCR after DNase treatment - (reply: 4)
  288. PCR amplification with high fidelity enzyme - (reply: 1)
  289. PCR HELP!!!! - (reply: 1)
  290. Overlap PCR problem - (reply: 5)
  291. PCR and restriction enzyme digestion - (reply: 3)
  292. Bisulfite PCR and cloning - (reply: 5)
  293. PCR product purification - (reply: 4)
  294. Overlapping sequence PCR primers - (reply: 1)
  295. restricted PCR plasmid runs slower - (reply: 2)
  296. BSP PCR primer design explained - (reply: 11)
  297. Bisulfite sequencing PCR worked - (reply: 5)
  298. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  299. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  300. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  301. doing PCR using PCR product , help plz !! - (reply: 5)
  302. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  303. good 16S univ. primer for qPCR? - (reply: 3)
  304. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  305. Amplification of CAG promoter problems - (reply: 4)
  306. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  307. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  308. I need help for designing a pair of primers for this sequence. - (reply: 1)
  309. PCR not working for 5 Aza treated DNA - (reply: 3)
  310. Differentiating mouse from human cells with PCR - (reply: 5)
  311. Differentiate between RT-PCR and PCR - (reply: 3)
  312. PCR band slightly BELOW expected length ?! - (reply: 14)
  313. long primers PCR - (reply: 4)
  314. unexpected band in PCR with plasmid - (reply: 4)
  315. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  316. DNA polymerase for GC rich template? - (reply: 4)
  317. Sequencing Primers and Plasmids - (reply: 3)
  318. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  319. DNA extraction - PCR Problem - (reply: 3)
  320. Primers to amplify Kan operon from pet28 - (reply: 2)
  321. PCR product biotinylation - (reply: 3)
  322. Tagged primers and a second PCR targetting the tags - (reply: 5)
  323. cloning pcr insert into plasmid - (reply: 2)
  324. Barcode generator for PCR primers - (reply: 2)
  325. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  326. Barcoding PCR products - (reply: 18)
  327. Biotinylated primers - (reply: 4)
  328. role of water in PCR - (reply: 3)
  329. HIV gene- PCR - (reply: 3)
  330. Real time PCR melting curve - (reply: 2)
  331. BSA in PCR reaction - (reply: 1)
  332. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  333. Cannot see insert with colony pcr - (reply: 6)
  334. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  335. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  336. How to Join two PCR products - (reply: 3)
  337. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  338. What's wrong with my PCR - (reply: 5)
  339. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  340. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  341. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  342. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  343. Cp, but no amplification - (reply: 2)
  344. pcr product digestion problem - (reply: 4)
  345. Low PCR product - (reply: 7)
  346. Control primers for bisulfite-converted DNA - (reply: 3)
  347. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  348. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  349. wrong dntp concentration - (reply: 2)
  350. Third round PCR - (reply: 2)
  351. long fragment PCR - (reply: 2)
  352. Best proof-reading polymerase? - (reply: 12)
  353. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  354. Q regarding primer mix for MSP!! - (reply: 4)
  355. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  356. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  357. I need to break this cycle of PCR issues - (reply: 13)
  358. Questions / Help with Fusion PCR - (reply: 8)
  359. Controls for RT-PCR reactions - (reply: 2)
  360. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  361. BSP Primers with M13 Tails - (reply: 1)
  362. Detection of T7 RNA polymerase by WB - (reply: 6)
  363. Multiplex PCR - (reply: 33)
  364. issues when designing PCR primers using PRIMER3 - (reply: 8)
  365. Primer catalog/databasing - (reply: 1)
  366. Nonrepetitive nested PCR - (reply: 2)
  367. Primer Blast - (reply: 6)
  368. Help designing primers for use in In-fushion cloning - (reply: 4)
  369. PCR internal control - (reply: 2)
  370. Primers Freeze-Thaw - (reply: 16)
  371. TDNA Genotyping PCR program - (reply: 1)
  372. primer design using 16s rRNA - (reply: 1)
  373. question about PCR and cloning - (reply: 1)
  374. PCR - (reply: 2)
  375. nonspecific band at 50bp in PCR - (reply: 9)
  376. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  377. PCR master mix - (reply: 7)
  378. Maximum PCR product Tm - (reply: 6)
  379. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  380. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  381. PCR & Southern blotting - (reply: 1)
  382. Bisulfite PCR Question - (reply: 2)
  383. Primer Design HELP! - (reply: 3)
  384. Help with sequencing of a 120bp PCR product. - (reply: 9)
  385. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  386. How are Primers made? - (reply: 9)
  387. Long Non-Specific PCR Products - (reply: 5)
  388. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  389. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  390. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  391. PCR calculation - (reply: 1)
  392. Colony PCR and digestion with KpnI - (reply: 10)
  393. designing methylation specific primers - (reply: 3)
  394. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  395. PCR - consistent false positive results - (reply: 6)
  396. PCR no amplification - (reply: 15)
  397. How to quantify virus by PCR - (reply: 12)
  398. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  399. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  400. Pcr product size determination for a fusion gene - (reply: 8)
  401. Quantify DNA before ChIP PCR - (reply: 3)
  402. Primer Annealing Temperatures - (reply: 24)
  403. my pcr product is larger than expected - (reply: 4)
  404. Question about PCR - DNA or RNA as template - (reply: 4)
  405. pcr purification - (reply: 1)
  406. pcr purification doubt - (reply: 4)
  407. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  408. PCR band moves lower than its size on agarose gel - (reply: 3)
  409. same primers, different product in conventional PCR and qPCR - (reply: 6)
  410. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  411. pcr problem - (reply: 6)
  412. NEB Q5 polymerase works very well - (reply: 1)
  413. Primers amplify genomic DNA but not cDNA - (reply: 2)
  414. pcr purification - (reply: 1)
  415. storing PCR product overnight - (reply: 2)
  416. Primer BLAST - (reply: 1)
  417. Bands in negative control PCR - (reply: 2)
  418. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  419. problem with pcr mutagenesis - (reply: 10)
  420. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  421. Use PCR purification kit to purify restriction digestion - (reply: 2)
  422. PCR primer design - published primers trustable? - (reply: 8)
  423. Polymerase for E. coli screenings - (reply: 5)
  424. complicated RT-PCR issues - (reply: 4)
  425. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  426. Can we use degenerate bases in BSP primers? - (reply: 1)
  427. Quick change mutagenesis No PCR product visible - (reply: 2)
  428. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  429. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  430. Can anyone help with my Bisulfite PCR? - (reply: 2)
  431. How to check primer sequences using BLAST and other tools? - (reply: 2)
  432. real time PCR analysis in patient samples - (reply: 2)
  433. carpet in gels for PCR products - (reply: 2)
  434. Primer Check? - (reply: 2)
  435. Sequencing of the PCR production in BSP - (reply: 1)
  436. amplification of 14Kb - (reply: 1)
  437. Validation of PCR primers/ probes - (reply: 6)
  438. Taq polymerase for MSP - (reply: 1)
  439. Real time PCR sudenly not working - (reply: 1)
  440. Buffer-composition One-Step RT-PCR - (reply: 4)
  441. Two reverse primer sequences for a single forward primer - (reply: 1)
  442. PCR with more than one primer - (reply: 1)
  443. RAPD - PCR problem - (reply: 5)
  444. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  445. How to best store PCR product? - (reply: 2)
  446. RT-PCR primer design - (reply: 1)
  447. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  448. Odd gel run following PCR - (reply: 28)
  449. Qiagen PCR Array Reagents? - (reply: 1)
  450. No or very weak band using 16s primers - (reply: 1)
  451. No PCR amplification with b-actin primers - (reply: 1)
  452. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  453. Site-directed, PCR works but no colonies - (reply: 2)
  454. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  455. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  456. No PCR amplified with long primers - (reply: 10)
  457. PCR optimization: PCR vs qPCR - (reply: 4)
  458. should I do blunt end my pcr product before ligation - (reply: 2)
  459. PCR smear for genomic samples - (reply: 4)
  460. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  461. another primer dilution question - (reply: 2)
  462. i need help with virus pcr - (reply: 2)
  463. Primer Efficiency - (reply: 2)
  464. Experimental set up for qRT PCR - (reply: 13)
  465. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  466. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  467. How should I optimize my PCR - (reply: 2)
  468. Second round of PCR after gel extraction fails miserably - (reply: 3)
  469. an effective way to do a yeast colony pcr - (reply: 2)
  470. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  471. urgent help plz with RT-PCR - (reply: 3)
  472. oligo(dt) 15 vs random primers - (reply: 3)
  473. Help to optain a long PCR produc - (reply: 3)
  474. Multiple bands in eluted PCR product - (reply: 1)
  475. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  476. Problems with two step RT-PCR - (reply: 2)
  477. Getting genomic DNA for PCR - (reply: 4)
  478. primer dilutions - (reply: 4)
  479. Genotyping PCR primers - (reply: 1)
  480. Conventional PCR - (reply: 1)
  481. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  482. PCR product loss on cleanup - (reply: 2)
  483. MSP Primers not working - (reply: 6)
  484. Primers too dilute - (reply: 1)
  485. Amplification curves jagged - (reply: 1)
  486. Cloning HELP- Possible Primer issues - (reply: 6)
  487. No PCR product in site-directed mutagenesis - (reply: 4)
  488. Giardia/Crypto real time PCR late amplification - (reply: 1)
  489. How to dilute the template DNA for PCR - (reply: 7)
  490. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  491. Need help for PCR - (reply: 5)
  492. PCR primer pairs - university exam - (reply: 7)
  493. unespecific band PCR from mouse genomic DNA - (reply: 9)
  494. Help with primer design through PRIMER3 - (reply: 4)
  495. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  496. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  497. Plasmid vs cDNA amplification by PCR - (reply: 2)
  498. Tm of primers - prediction and verification - (reply: 1)
  499. PCR cloning...help! - (reply: 5)
  500. RT-PCR contamination in negative control. - (reply: 1)
  501. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  502. how to design primers for 16sr RNA - (reply: 24)
  503. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  504. Help with primer design - (reply: 3)
  505. Primer Tm calculation. - (reply: 1)
  506. low concentration of purified PCR product - (reply: 2)
  507. PCR smearing and...problems! - (reply: 7)
  508. Running Primers on Agarose Gel - (reply: 6)
  509. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  510. universal primers for PCR ribotyping - (reply: 6)
  511. will the template of PCR be added A ? - (reply: 2)
  512. Colony PCR not working - (reply: 4)
  513. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  514. Primer design: Tm above or below Ta? - (reply: 5)
  515. Oligo primers for shRNA construction - (reply: 1)
  516. Design primers for expression cloning - (reply: 11)
  517. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  518. primer dilution query - (reply: 2)
  519. Real Time PCR polymerase enzyme - (reply: 3)
  520. Estimating size of PCR products in a agarose gel - (reply: 4)
  521. Sequencing of PCR product - (reply: 4)
  522. PCR contamination problem - (reply: 2)
  523. Using BLAST to check primers - (reply: 1)
  524. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  525. What is the function of a third primer in a PCR mix?? - (reply: 2)
  526. How can we design a primer ? step by step method - (reply: 4)
  527. cDNA synthesis or RT-PCR - (reply: 3)
  528. PCR on cDNA and SGamplification??? - (reply: 1)
  529. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  530. real time PCR protocol - controls? - (reply: 2)
  531. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  532. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  533. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  534. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  535. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  536. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  537. PCR and UV spectrophotometry - (reply: 3)
  538. RT-PCR in a single tube - (reply: 3)
  539. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  540. annealing temperature for 3 primer pairs? - (reply: 4)
  541. PCR Reaction - (reply: 3)
  542. Impact of primer sequence on TA cloning - (reply: 3)
  543. problems with Bisulfite PCR - (reply: 1)
  544. low pcr product - (reply: 3)
  545. overlapping PCR protocol - (reply: 12)
  546. PCR to amplify my insert not working - (reply: 6)
  547. Problem with PCR amplification- high 3' stability - (reply: 3)
  548. Home made real-time PCR problem - (reply: 4)
  549. High RNase concentration interfering with PCR? - (reply: 8)
  550. Pcr primers - (reply: 2)
  551. PCR failure from yeast genomic DNA - (reply: 1)
  552. failure of PCR from yeast genomic DNA - (reply: 1)
  553. RT-Q PCR primer design question - (reply: 2)
  554. high MW dimers - (reply: 2)
  555. Strange RT-PCR amplification plots - (reply: 9)
  556. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  557. What products will I get from this pcr? - (reply: 1)
  558. PCR buffer without Tris - (reply: 1)
  559. PCR Water - (reply: 4)
  560. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  561. Which Brand of real-time PCR machine - (reply: 6)
  562. Problem with PCR - large extra bands - (reply: 3)
  563. big differnece in my primers Tm - (reply: 2)
  564. Designing primers for the ORFs - (reply: 9)
  565. Two-Step PCR - (reply: 2)
  566. Gradient PCR - (reply: 2)
  567. PCR with phophorylated primers - (reply: 1)
  568. Problem with New qPCR primers - help! - (reply: 6)
  569. Designing primers for miRNA located on minus strand. - (reply: 2)
  570. Plasmid sequencing without primers - (reply: 1)
  571. Smallest insert in PCR - (reply: 6)
  572. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  573. loop-mediated amplification - (reply: 2)
  574. Blunt end PCR product ligation - (reply: 1)
  575. Help with high Ct values for Real-Time PCR - (reply: 1)
  576. Genotyping by allele specific PCR - (reply: 6)
  577. RT-PCR primers and the poly(A) tail - (reply: 2)
  578. NO Insert found in the Colony PCR - (reply: 15)
  579. No band in PCR - (reply: 5)
  580. overlapping primer - (reply: 1)
  581. PCR primers help - (reply: 5)
  582. PCR smear after one-month storage - (reply: 2)
  583. Primer dimers - (reply: 2)
  584. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  585. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  586. Simple question of dNTP's for PCR - (reply: 5)
  587. Need help for PCR for AT rich gene - (reply: 3)
  588. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  589. Primer validation and PCR efficiency - (reply: 4)
  590. How to calculate the price of PCR test per sample? - (reply: 6)
  591. Contamination in negative control of PCR (No template control) - (reply: 6)
  592. cloning with nested pcr - (reply: 3)
  593. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  594. PCR is amplifying non-specific fragment - (reply: 6)
  595. ChIP amplification problems - (reply: 1)
  596. real time RT-PCR problems - (reply: 2)
  597. should I design primers spanning both intron and exon - (reply: 2)
  598. RT-PCR - (reply: 1)
  599. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  600. Template DNA (plasmid) concentration for PCR - (reply: 4)
  601. PCR with a very long and a short primer - (reply: 5)
  602. Ligation of 16kb PCR product - (reply: 5)
  603. Multiplex PCR does not work - (reply: 3)
  604. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  605. Problem with genomic PCR (2.4 kb) - (reply: 1)
  606. Comparison between two primers pairs? Is it possible? - (reply: 3)
  607. Rt-PCR primer design - (reply: 7)
  608. Length of non-binding sequence in primers with RE site - (reply: 6)
  609. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  610. Getting a smaller PCR product than required - (reply: 6)
  611. Difficult sequences in PCR - (reply: 2)
  612. why is real time pcr curve like this? - (reply: 3)
  613. PCR inconsistency - (reply: 2)
  614. Orientation of primers - (reply: 2)
  615. PCR, neg control & no. of cycles - (reply: 2)
  616. Strange PCR result from genomic DNA - (reply: 3)
  617. Bright bands of PCR products stick in gel wells - (reply: 1)
  618. methylation analysis with MS-HRM (primer design) - (reply: 1)
  619. whole plasmid pcr? - (reply: 6)
  620. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  621. salts in PCR - (reply: 4)
  622. Strange amplification plot - (reply: 4)
  623. Conventional PCR of different dilutions - (reply: 1)
  624. primer resuspension - (reply: 2)
  625. Principles of overlap PCR - (reply: 7)
  626. ligation of pcr product into expression vector - (reply: 4)
  627. Unable to PCR!!!! - (reply: 3)
  628. Help designing primers with restriction sites - (reply: 3)
  629. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  630. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  631. Abnormal amplification curve - (reply: 1)
  632. Problem with Semiquantitative PCR - (reply: 4)
  633. Advice on optimising bisulfite PCR sought - (reply: 2)
  634. Help: I am loosing the DNA during PCR purification - (reply: 7)
  635. PCR primer with no extra DNA end - (reply: 5)
  636. PCR colonies of Pichia pastoris - (reply: 1)
  637. ROX reference dye for quantitative RT-PCR - (reply: 1)
  638. PCR problem - (reply: 9)
  639. why PCR product smeared? - (reply: 2)
  640. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  641. silincing box, no amplification - (reply: 3)
  642. Comparative semiquantitative RT-PCR - (reply: 2)
  643. Bands in my PCR Controls - (reply: 3)
  644. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  645. Strange band in colony PCR - (reply: 2)
  646. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  647. Multiplex PCR - (reply: 12)
  648. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  649. PYO pcr stopped working. - (reply: 1)
  650. Bands seen with qPCR missing in regular PCR - (reply: 5)
  651. checking msp primers - (reply: 2)
  652. PCR product one day, none the next day - (reply: 4)
  653. PCR on colony - (reply: 10)
  654. PCR reaction ISSUES???? need help - (reply: 4)
  655. PCR using CDNA as template - (reply: 2)
  656. RT-PCR doesn't work with all RNA used - (reply: 1)
  657. Need help amplifying repeating sequence. - (reply: 5)
  658. Real Time PCR 101...help! - (reply: 1)
  659. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  660. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  661. Another primer dimers problem - (reply: 23)
  662. Colony PCR Problem!!!! - (reply: 5)
  663. using PCR product as standard template - (reply: 3)
  664. PCR kit - (reply: 7)
  665. Make additional cycles with an already finished pcr product - (reply: 3)
  666. Amplification in water control but not in samples - (reply: 3)
  667. PCR products form with DNA, but not with c-DNA - (reply: 2)
  668. question about pcr amplification efficiency? - (reply: 1)
  669. DNA polymerase: recombinant or native? - (reply: 3)
  670. pcr clean-up fail? - (reply: 1)
  671. MSP U primers very difficult to amplify... - (reply: 4)
  672. No gene amplification, 18s amplification is fine - (reply: 1)
  673. ChIP-qPCR gives odd amplification plot - (reply: 5)
  674. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  675. sequential cloning of multiple PCR products - how to do? (reply: 3)
  676. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  677. Primer design - (reply: 3)
  678. Tomato Actin PCR+Images included - (reply: 2)
  679. single primer PCR - (reply: 5)
  680. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  681. cDNA synthesis + amplification - (reply: 2)
  682. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  683. LacZ PCR Problems - (reply: 1)
  684. cells stably expressing T7 RNA polymerase - (reply: 1)
  685. Primer concentration - (reply: 4)
  686. nested PCR with low target DNA? - (reply: 2)
  687. RT-PCR calibrator - (reply: 2)
  688. RNA - extraction and pcr (reply: 1)
  689. problem in pcr - (reply: 2)
  690. Can someone recommend me a virtual PCR software? - (reply: 6)
  691. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  692. Primer 3' mismatch - Strange experience (reply: 9)
  693. real-time PCR after restriction enzyme digestion - (reply: 6)
  694. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  695. Adding loading/tracking dye to PCR mix? - (reply: 10)
  696. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  697. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  698. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  699. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  700. how to get rid of non specific bands in PCR - (reply: 1)
  701. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  702. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  703. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  704. PCR cloning problem - could not get colony (reply: 4)
  705. pcr contamination - (reply: 4)
  706. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  707. Large Non-specfic bands in PCR - (reply: 3)
  708. PCR clonning - (reply: 3)
  709. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  710. 2 bands in blue colony PCR - (reply: 5)
  711. Primer dilution --> problem.. - (reply: 16)
  712. Help! Smear in Real-Time PCR Product - (reply: 1)
  713. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  714. Sybr green RT-qPCR primer - (reply: 1)
  715. Sybr green RT-qPCR primer - (reply: 1)
  716. primer design - microalgae (reply: 2)
  717. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  718. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  719. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  720. PCR yield in ng/ul - (reply: 1)
  721. No Amplification in PCR - (reply: 4)
  722. Real time PCR and percentage loss - (reply: 1)
  723. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  724. Problem with smear in PCR with certain templates - (reply: 4)
  725. Real time PCR in the presence of heme - (reply: 3)
  726. issues in PCR amplification - (reply: 2)
  727. Problems with PCR in general - (reply: 1)
  728. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  729. how to overcome primer contamination - need protocol (reply: 11)
  730. RT-PCR primer design - Primer design and evaluation (reply: 7)
  731. CHIP analysis by PCR - (reply: 4)
  732. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  733. Primer,probes dilution - (reply: 3)
  734. Splice varient quantification in RT real time PCR? - (reply: 1)
  735. negative control for msp pcr - (reply: 2)
  736. Calculating geometric mean for real-time PCR - (reply: 5)
  737. template transfer? - how does the rna polymerase switches template? (reply: 1)
  738. PCR Gel nonspecific band - (reply: 13)
  739. semiquantitative pcr - (reply: 1)
  740. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  741. PCR: Cloning Primers - (reply: 8)
  742. PCR product - (reply: 5)
  743. pcr amplify an insert in plasmid? - (reply: 2)
  744. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  745. Exon Spanning PCR Primers - (reply: 1)
  746. Colony PCR - (reply: 1)
  747. PCR to confirm double crossover - (reply: 1)
  748. Primer sequences - (reply: 1)
  749. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  750. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  751. PCR Primers - (reply: 2)
  752. Cleaning up RT-PCR product before sequencing - (reply: 1)
  753. Template Amount for PCR Amplification - (reply: 6)
  754. How to blast methylation specific and unspecific primers - (reply: 1)
  755. RT-PCR cDNA synthesis - (reply: 6)
  756. problems amplifying from cDNA - (reply: 4)
  757. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  758. No DNA after PCR purification with QiaQuick? - (reply: 4)
  759. restriction digestion against colony PCR - (reply: 1)
  760. How to check primers are of correct sequence or not? - (reply: 2)
  761. Primers... - (reply: 1)
  762. When are primer dimers a problem? - (reply: 1)
  763. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  764. PCR: always got band in the negative control - (reply: 8)
  765. primer with higher melting temperature - (reply: 2)
  766. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  767. Problems designing primers for BSP - (reply: 2)
  768. qPCR primer design - possible off-target (reply: 1)
  769. Primer dimer formation and real-time PCR - (reply: 7)
  770. Identify PCR products - (reply: 3)
  771. PCR genomic DNA using cell lysate - (reply: 1)
  772. Maximum size of overhangs in PCR - (reply: 6)
  773. Colony PCR doesn't work anymore - (reply: 5)
  774. RT-PCR t-test, and ANOVA - (reply: 1)
  775. Primer Design Urgent! - (reply: 1)
  776. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  777. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  778. PCR of microRNA first strand cDNA - (reply: 1)
  779. how to know my primer's sense ??? - help ! (reply: 2)
  780. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  781. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  782. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  783. intron spanning primers for non model organisms - (reply: 2)
  784. Primer design - (reply: 2)
  785. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  786. qPCR amplification interference fixed with freezing - (reply: 6)
  787. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  788. software for quantifing PCR product band - (reply: 1)
  789. Multiple bands from purified PCR product - (reply: 3)
  790. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  791. Doing PCR on Nebulized DNA - (reply: 2)
  792. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  793. RT-PCR - (reply: 9)
  794. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  795. inverse pcr - (reply: 2)
  796. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  797. Primer seq - (reply: 2)
  798. Urgent: should you dry PCR product - (reply: 13)
  799. PCR product size - (reply: 6)
  800. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  801. oligo dt and random primer - (reply: 3)
  802. mRNA Search for RT-PCR (U to T) - (reply: 2)
  803. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  804. PCR Mastermix - (reply: 4)
  805. PCR triplicates versus one reaction - differences? (reply: 17)
  806. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  807. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  808. Primers and self and hetero dimers - (reply: 1)
  809. Really weird amplification curves - (reply: 1)
  810. Primers for qPCR - (reply: 3)
  811. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  812. RT-PCR primer - (reply: 3)
  813. PCR problem - basic PCR for plasmid amplification (reply: 2)
  814. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  815. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  816. Unpredictablity of PCR product - (reply: 5)
  817. total cDNA amplification by PCR - (reply: 2)
  818. Detection limit of a conventional PCR - (reply: 2)
  819. PCR for MULTIPLE mutagenesis - (reply: 4)
  820. Using Sybr green in realtime pcr - (reply: 1)
  821. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  822. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  823. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  824. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  825. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  826. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  827. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  828. Digested PCR product migrate slower than uncut - (reply: 8)
  829. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  830. PCR question - (reply: 2)
  831. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  832. 2 rounds PCR got problem - (reply: 2)
  833. PCR help minus strand - Primer design (reply: 1)
  834. chloramphenicol storage and amplification - (reply: 3)
  835. Smear in long distance PCR - (reply: 39)
  836. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  837. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  838. TOUCH DOWN PCR - (reply: 2)
  839. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  840. PCR stopped working - After changing buffer (reply: 5)
  841. ChIP PCR question - (reply: 33)
  842. PCR protocol questions! - (reply: 2)
  843. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  844. DNA amount calculation for PCR - (reply: 14)
  845. ChIP primer design - (reply: 1)
  846. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  847. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  848. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  849. How to analyze ChIP PCR data - (reply: 10)
  850. Questions regarding RT-PCR optimization - (reply: 2)
  851. number of copies after pcr? - (reply: 1)
  852. PCR double-bands - (reply: 1)
  853. Problems with semi-quatitative PCR - (reply: 1)
  854. Question about Primers - (reply: 3)
  855. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  856. Large fragment amplification failed - (reply: 3)
  857. purification of PCR product for cloning in a vector - (reply: 3)
  858. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  859. PCR reaction calculation - (reply: 2)
  860. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  861. Primer design with a tag - (reply: 4)
  862. no increase in fluorescence in my real time PCR - (reply: 3)
  863. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  864. Help with real-time PCR quantification of miRNA - (reply: 8)
  865. PCR, using gel extracted band as a template - (reply: 4)
  866. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  867. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  868. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  869. Combining forward and reverse primer gives different size on gel - (reply: 2)
  870. Q-PCR and RT-PCR - (reply: 3)
  871. calculating total amount - in a pcr reaction (reply: 4)
  872. Problems with amplifying microRNA - (reply: 3)
  873. PCR of complement genomic DNA - (reply: 3)
  874. primers for fungal identification - (reply: 9)
  875. confusing PCR - (reply: 1)
  876. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  877. PCR for long and repetitive region from genomic DNA - (reply: 4)
  878. difference between PCR primers and sequencing primers - (reply: 1)
  879. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  880. PCR of repeated region - (reply: 1)
  881. Sense and antisense DNA, and primer design - (reply: 6)
  882. PCR bands in NTC but NOT in negative samples - (reply: 2)
  883. housekeeping genes in PCR - (reply: 1)
  884. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  885. Question about RealTime PCR Primer Design - (reply: 1)
  886. Designing a Primer with a RS that has a W - (reply: 1)
  887. Primers stopped working!? - PCR Primers (reply: 6)
  888. NFK beta primers - (reply: 4)
  889. final PCR product - need clarifications (reply: 1)
  890. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  891. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  892. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  893. RT-PCR result !! - (reply: 4)
  894. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  895. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  896. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  897. why genomic can't be use for pcr? - (reply: 2)
  898. Primer design - Primer design (reply: 2)
  899. Re-use unamplified PCR product - (reply: 2)
  900. Reason for odd PCR conditions - (reply: 3)
  901. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  902. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  903. pcr - problem in pcr (reply: 2)
  904. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  905. How to present and analyze these real-time PCR data? - (reply: 2)
  906. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  907. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  908. PCR Master mix - (reply: 2)
  909. cDNA storage and real time RT PCR - (reply: 3)
  910. Real Time PCR Primers and Probes - (reply: 2)
  911. Anyone make their own PCR cloning vector? - (reply: 5)
  912. Stability of PCR T-overhangs - (reply: 1)
  913. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  914. PCR product wrong size! Need help! - (reply: 9)
  915. pooling different pcr cycles? - (reply: 1)
  916. Amplification of concatenated linear ligated fragments - (reply: 4)
  917. Clinical Real-time PCR assay - (reply: 1)
  918. Need help - How we can design primer for miRNA (reply: 1)
  919. primer and promoter sequences - (reply: 1)
  920. Standard curve for real-time PCR - (reply: 2)
  921. designing of primers - (reply: 2)
  922. Primer Tm differences/ PCR - (reply: 1)
  923. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  924. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  925. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  926. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  927. Designing primer to remove his-tag - (reply: 1)
  928. PCR Primer Dilution from F+R - (reply: 1)
  929. primer reconstitution - primer reconstitution (reply: 4)
  930. Not-Quite-Nested PCR - (reply: 2)
  931. PCR product too short - (reply: 6)
  932. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  933. Primer design for insert amplification - Is this correct? (reply: 2)
  934. got smear on the PCR gel !!! - (reply: 4)
  935. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  936. "Unzipped" PCR band - (reply: 3)
  937. Review on new amplification techniques - (reply: 2)
  938. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  939. DOT BLOT for pcr product - No result obtain (reply: 1)
  940. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  941. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  942. HRM primers - (reply: 1)
  943. PCR amplification with Pfu / quality of DNA - (reply: 4)
  944. primer design - (reply: 2)
  945. Is it possible to compare data between real-time PCR plates - (reply: 1)
  946. Conventional PCR problems - wisamni2004 (reply: 2)
  947. New to sequencing, primer design - (reply: 2)
  948. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  949. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  950. incorrect/smaller band size for real time PCR - (reply: 1)
  951. Quickchange mutagenesis primer - (reply: 3)
  952. pcr / primer theory in bisulfite sequencing - (reply: 4)
  953. homologous amplicon for real time pcr - (reply: 1)
  954. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  955. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  956. assistance with PCR amplification please - (reply: 3)
  957. I need an urgent help with q-pcr amplification plots - (reply: 11)
  958. Problems with the Specificity of the Primers - (reply: 2)
  959. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  960. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  961. Degenerate primers PCR problem, Please help! - (reply: 2)
  962. Hotstart PCR and unspecific Amplification - (reply: 3)
  963. Primer design help needed - (reply: 1)
  964. Primers no longer work - (reply: 1)
  965. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  966. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  967. RT PCR for known snps detection? - SNPs detection (reply: 1)
  968. colony PCR - (reply: 2)
  969. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  970. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  971. PCR reaction without template gives a product - (reply: 6)
  972. PCR wrong product size - (reply: 6)
  973. Wrong pcr product size - (reply: 4)
  974. real time PCR machine - (reply: 5)
  975. PCR very faint product - (reply: 1)
  976. Question about principle of PCR - (reply: 3)
  977. PCR amplification before bisulfite conversion - (reply: 6)
  978. 4 question for real time PCR - (reply: 5)
  979. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  980. PCR didn't work - suspect the enzyme (reply: 2)
  981. Find universal primers in vector - any online tool? (reply: 6)
  982. Picking primers to confirm Illumina meth27 results - (reply: 2)
  983. Colony PCR Specificity - (reply: 2)
  984. Colony Pcr - primers - (reply: 5)
  985. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  986. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  987. Primer Dilution Problem - D'oh! (reply: 2)
  988. Using PCR to create overhangs - (reply: 3)
  989. Creating overhangs with PCR - (reply: 4)
  990. Problem with PCR on bacmids - (reply: 1)
  991. Is there any software that can be sued to design degenerate primers - (reply: 1)
  992. Running real time pcr product on gel?.. - (reply: 4)
  993. Primer Decontamination - (reply: 2)
  994. PCR mutagenesis of plasmid - (reply: 5)
  995. tools for checking non-specific binding of primers -
    (reply: 1)
  996. Primer designed for ARMS PCR - (reply: 2)
  997. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  998. methylation specific PCR - Primers - (reply: 1)
  999. bacterial identification using real time PCR - (reply: 1)
  1000. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1001. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1002. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1003. colony PCR for subcloning gene - (reply: 1)
  1004. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1005. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1006. PCR reamplification - (reply: 2)
  1007. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1008. How many colonies to screen (colony PCR)? - (reply: 4)
  1009. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1010. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1011. question about pcr - (reply: 3)
  1012. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1013. How to construct a standard curve for real time PCR - (reply: 1)
  1014. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1015. PCR with one primer - (reply: 1)
  1016. colony PCR inconsistent - (reply: 4)
  1017. PCR contamination - (reply: 19)
  1018. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1019. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1020. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1021. PCR not working - No amplification - (reply: 5)
  1022. Quantification of PCR products - (reply: 1)
  1023. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1024. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1025. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1026. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1027. Nested PCR question - (reply: 1)
  1028. High Tm on primers - cannot get a product - (reply: 8)
  1029. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1030. undesired products in multiplex PCR - (reply: 3)
  1031. NCBI primer blast problem - (reply: 1)
  1032. Help with Multiplex Nested PCR - (reply: 2)
  1033. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1034. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1035. primer sequence problem - (reply: 2)
  1036. Finding bisulfite PCR primer sequence - (reply: 3)
  1037. PCR -No band formation - (reply: 1)
  1038. PCR - No Band formation - (reply: 3)
  1039. No amplification after bisulfite treatment - (reply: 4)
  1040. PCR AMPLIFICATION - (reply: 1)
  1041. Ligation of Blunt PCR Product - (reply: 1)
  1042. real time pcr need advise - (reply: 2)
  1043. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1044. PCR & Cloning - (reply: 6)
  1045. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1046. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1047. DNA pooling for PCR - Saving money PCR (reply: 7)
  1048. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1049. PCR need some help - (reply: 4)
  1050. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1051. Left polymerase out overnight - Will it still work? (reply: 1)
  1052. Primer Reconstitution--does temperature matter? - (reply: 5)
  1053. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1054. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1055. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1056. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1057. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1058. no PCR product from input... - (reply: 8)
  1059. MSP - unwanted amplification - (reply: 5)
  1060. Smears in Bisulfite seq. PCR - (reply: 3)
  1061. Primer design for qPCR - (reply: 2)
  1062. Primer design for qPCR - (reply: 1)
  1063. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1064. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1065. Multiple bands after bisulfite PCR - (reply: 7)
  1066. PCR genomic DNA of high GC content - (reply: 6)
  1067. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1068. primer design tips - (reply: 3)
  1069. PCR with Biotin Incorporation - (reply: 2)
  1070. pcr product - hello all (reply: 1)
  1071. design pcr primer - (reply: 1)
  1072. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1073. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1074. PCR with long and complex primers - (reply: 2)
  1075. Phospholylation of primers - (reply: 1)
  1076. apoptosis by PCR? - (reply: 12)
  1077. Re-using PCR plates? - (reply: 2)
  1078. Touchdown PCR issues - (reply: 3)
  1079. LINE1 OFR2 Primer Design - (reply: 1)
  1080. phosphorylation of primers using PNK - (reply: 1)
  1081. RT-PCR contamination issue - (reply: 3)
  1082. Primers for yeast (18S) - (reply: 1)
  1083. How to reduce the bisulfite PCR bias? - (reply: 4)
  1084. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1085. Primer Concentration to lower Ct - (reply: 1)
  1086. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1087. Primer Concentration Help - (reply: 4)
  1088. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1089. Primer Degradation - What exactly happens? (reply: 1)
  1090. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1091. PCR/DNA Extraction Problem - (reply: 5)
  1092. Primers in 2 exons - (reply: 2)
  1093. NTC appear in real time pcr - (reply: 6)
  1094. Problems with designing a primer - (reply: 2)
  1095. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1096. PCR of AT-rich DNA - (reply: 1)
  1097. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1098. Overlapping PCR - really need help ! - (reply: 5)
  1099. Freezing PCR product??? - (reply: 1)
  1100. problem with pcr cloning from mouse cDNA - (reply: 2)
  1101. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1102. PCR with Platinum Taq - product yield issues - (reply: 3)
  1103. real time PCR - (reply: 7)
  1104. Different sized product for same BS primer! - (reply: 2)
  1105. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1106. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1107. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1108. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1109. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1110. primers with restriction site - (reply: 1)
  1111. PCR product appear two close band in my gel - (reply: 15)
  1112. The Case of a Missing Band: PCR Issue - (reply: 4)
  1113. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1114. cDNA as PCR template - (reply: 1)
  1115. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1116. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1117. paranormal qpcr amplification activity - melting curves (reply: 3)
  1118. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1119. PCR template concentration - (reply: 6)
  1120. Bands in PCR negative control - (reply: 3)
  1121. PCR efficiency calculated by Linreg - (reply: 3)
  1122. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1123. primer design by generunner DG - (reply: 1)
  1124. degenerate pcr - (reply: 1)
  1125. BSP primers design, help please - (reply: 2)
  1126. primers with fluoresent dye PCR cycle question - (reply: 4)
  1127. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1128. Smears on PCR products - (reply: 4)
  1129. Dumb Reverse Transcription PCR Question - (reply: 2)
  1130. Reverse transcription PCR - (reply: 1)
  1131. PCR/RT-PCR beads - (reply: 2)
  1132. High Amplification Efficiency in Std. Curve - (reply: 5)
  1133. Non specific products in Bisulphite pcr - (reply: 4)
  1134. Run PCR amplifications in agarose gels - (reply: 3)
  1135. Problems with validating primers and low expression genes - (reply: 3)
  1136. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1137. Bisulfite sequencing PCR not working - (reply: 5)
  1138. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1139. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1140. intron/exon spanning primers - (reply: 1)
  1141. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1142. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1143. PCR product for sequencing - sample storage?? (reply: 2)
  1144. Absolute quantification using Real Time PCR - (reply: 2)
  1145. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1146. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1147. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1148. MSP primer troubles - (reply: 3)
  1149. Measuring global methylation using real time PCR - (reply: 2)
  1150. Designing Primers for multiple Isoforms - (reply: 1)
  1151. RT-PCR standard curve dilutions - (reply: 2)
  1152. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1153. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1154. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1155. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1156. PCR product dimer issue - (reply: 24)
  1157. PCR additives Formamide - Formamide which one? (reply: 4)
  1158. PCR Master mix - (reply: 1)
  1159. Addition of Restriction sites into PCR primers - (reply: 4)
  1160. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1161. failure PCR amplification from low GC content gene - (reply: 5)
  1162. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1163. conventional v real-time PCR applications - (reply: 1)
  1164. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1165. PCR reagents, can I use reagents from different manufacturers? - (reply: 4)
  1166. different PCR primers for real-time and classic PCR - (reply: 3)
  1167. primers deconamination??? - (reply: 6)
  1168. PCR band too thick - PCR troubleshooting question (reply: 5)
  1169. RT-PCR problem - (reply: 1)
  1170. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1171. primer check!!! - (reply: 6)
  1172. PCR bands on gel electrophoresis - (reply: 5)
  1173. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1174. long range PCR - (reply: 2)
  1175. Annealing Temperature of biotinylated primers - (reply: 2)
  1176. RT-PCR - New to RT-PCR info (reply: 2)
  1177. PCR punch - (reply: 4)
  1178. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1179. PCR off plasmid for screening - (reply: 2)
  1180. PCR failed no product.. help - (reply: 10)
  1181. number of molecules in PCR - (reply: 3)
  1182. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1183. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1184. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1185. Primer dilution - PCR primer dilution (reply: 2)
  1186. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1187. Can't re-PCR the PCR product - (reply: 5)
  1188. PCR reagent autoclave - (reply: 2)
  1189. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1190. Long Primers for PCR - (reply: 2)
  1191. Data Analysis for Real-time PCR - (reply: 2)
  1192. PCR not working - overhangs too long? (reply: 3)
  1193. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1194. argh more pcr headaches! - (reply: 6)
  1195. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1196. Chlamydia PCR cycling conditions - (reply: 1)
  1197. Primers going "loopy" - (reply: 2)
  1198. Conversion of primer unit - ug/ul to uM (reply: 4)
  1199. mutagenesis by PCR or just use a kit - (reply: 5)
  1200. Unsuccessful BS PCR - (reply: 3)
  1201. Primer Trouble Shooting - (reply: 5)
  1202. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1203. Smearing on gel in PCR products - (reply: 5)
  1204. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1205. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1206. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1207. Relative RT-PCR - (reply: 2)
  1208. Relative real time RT PCR - (reply: 2)
  1209. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1210. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1211. a simple primer problem - (reply: 5)
  1212. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1213. no or poor amplification - (reply: 1)
  1214. Primers from 3'UTR - (reply: 2)
  1215. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1216. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1217. Designing primers with ESTs - (reply: 2)
  1218. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1219. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1220. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1221. Real Time vs Traditional PCR results - (reply: 1)
  1222. The problems to identify mouse genotype with PCR - (reply: 1)
  1223. addition of BclI restriction site to PCR primer - (reply: 6)
  1224. non specific amplification - DNA amplification by PCR (reply: 1)
  1225. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1226. How does methprimer calculate primer Tm - (reply: 2)
  1227. Laminar Flow vs PCR cabinet - (reply: 4)
  1228. How to do PCR to detect mRNA without RT? - (reply: 2)
  1229. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1230. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1231. PCR has stopped working - (reply: 7)
  1232. PCR negative control contamination - (reply: 8)
  1233. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1234. Phosphorylated primers - (reply: 5)
  1235. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1236. PCR efficiency in real timePCR - (reply: 5)
  1237. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1238. Drastic Decrease in PCR product yield - (reply: 2)
  1239. resources on PCR principles & technique - (reply: 1)
  1240. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1241. Colony Screeing without using PCR - (reply: 8)
  1242. High MW PCR band seen...help needed - (reply: 7)
  1243. QPCR - Primer and Probe question - QPCR (reply: 2)
  1244. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1245. standard curve/ PCR efficiency problems... - (reply: 1)
  1246. Help! PCR that used to work doesn't work now! - (reply: 2)
  1247. PCR and templates - (reply: 3)
  1248. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1249. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1250. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1251. Easy primer question? - Primers (reply: 1)
  1252. PCR inconsistency - (reply: 4)
  1253. Plasmid problem - From PCR product (reply: 7)
  1254. help needed: PCR a gene from genomic DNA - (reply: 2)
  1255. questions about pcr products after pooling - (reply: 3)
  1256. stiching/linking/sewing pcr - (reply: 3)
  1257. Human mtDNA amplification problems - (reply: 3)
  1258. primer dimer - proplems (reply: 1)
  1259. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1260. Nested PCR - (reply: 2)
  1261. pfu vs long pcr mix - (reply: 7)
  1262. weird PCR ask for help - (reply: 12)
  1263. Methylation specific DMSO PCR - (reply: 1)
  1264. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1265. No PCR product at all - (reply: 8)
  1266. PCR troubleshoooooting - primer dimer (reply: 1)
  1267. Primer efficiency test - (reply: 1)
  1268. primer software - (reply: 2)
  1269. Question on wired PCR - (reply: 1)
  1270. restriction digestion of PCR product - (reply: 7)
  1271. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1272. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1273. How to know in which exon a primer match? - (reply: 1)
  1274. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1275. Amplification of human genomic DNA - (reply: 2)
  1276. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1277. sequencing with forward/reverse primers - (reply: 7)
  1278. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1279. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1280. Adding A overhangs - primer design implications? - (reply: 1)
  1281. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1282. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1283. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1284. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1285. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1286. primer design problem - (reply: 1)
  1287. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1288. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1289. Problem with primer efficiency analysis - (reply: 4)
  1290. slan real time PCR system for validation of microarray results? - (reply: 1)
  1291. PCR unusualband at ~230bps - PCR (reply: 1)
  1292. DMSO or BSA for PCR - (reply: 6)
  1293. Whole Genome Amplification - (reply: 2)
  1294. primer design - (reply: 1)
  1295. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1296. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1297. designing primers for genes not sequenced yet - (reply: 2)
  1298. Tris Buffer for PCR reaction - why? (reply: 1)
  1299. PCR a plasmid protein - (reply: 2)
  1300. multiplex PCR - (reply: 4)
  1301. Realtime PCR machine - (reply: 2)
  1302. PCR set-up calculation nightmares. - (reply: 3)
  1303. Primer desing - (reply: 2)
  1304. Colony PCR - (reply: 3)
  1305. Standard curves for PCR efficiency. - (reply: 2)
  1306. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1307. Question about RAPD PCR - (reply: 2)
  1308. Looking for help with my PCR! - (reply: 3)
  1309. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1310. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1311. primer design - (reply: 9)
  1312. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1313. PCR without thermal cycler? - (reply: 9)
  1314. primer contamination - primer contaminated with ice (reply: 3)
  1315. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1316. RT-PCR problem - (reply: 4)
  1317. Only DNA ladder , No desired band in PCR - (reply: 4)
  1318. taq and PCR - (reply: 6)
  1319. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1320. PCR Efficiency - (reply: 2)
  1321. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1322. Long PCR and genomic DNA isolation problems - (reply: 2)
  1323. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1324. NTC with specific amplification - (reply: 1)
  1325. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1326. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1327. Primer3 vs Primer BLAST - (reply: 3)
  1328. PCR and then ligation - (reply: 7)
  1329. Primer design: free energy - (reply: 2)
  1330. Amplification in NTC and noRT controls - (reply: 5)
  1331. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1332. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1333. asymmetric PCR - (reply: 1)
  1334. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1335. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1336. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1337. trouble with pcr - (reply: 8)
  1338. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1339. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1340. Real time PCR results-interpretation - (reply: 1)
  1341. Real time PCR results-interpretation - (reply: 2)
  1342. Primer optimization for ChIP - (reply: 2)
  1343. sequencing primer: T7 or T7promoter? - (reply: 4)
  1344. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1345. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1346. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1347. primer tm - tm calculation for tagged primers (reply: 2)
  1348. Reusing 96 well plates for PCR - (reply: 2)
  1349. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1350. PCR and AFLP - (reply: 1)
  1351. Universal 16s rRNA primers needed - (reply: 3)
  1352. Identifying PCR Inhibitors - (reply: 10)
  1353. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1354. PCR + phusion enzyme = massive errors - (reply: 4)
  1355. long DNA amplification - (reply: 11)
  1356. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1357. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1358. adding koazak sequence to primer - (reply: 1)
  1359. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1360. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1361. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1362. Primer design if sequence is unknown for organism - (reply: 5)
  1363. adding C terminal tag to reverse primer - (reply: 1)
  1364. epitope tagging of pcr products - (reply: 1)
  1365. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1366. Real time PCR info - (reply: 4)
  1367. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1368. PCR from BAC - PCR from BAC (reply: 1)
  1369. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1370. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1371. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1372. How much product PCR?? - (reply: 3)
  1373. what primers should I use for DNA sequencing? please help - (reply: 2)
  1374. what primers should I use for DNA sequencing?? - (reply: 3)
  1375. Is my primer going to work for qRT-PCR? - (reply: 6)
  1376. Standard curve for RT-PCR - (reply: 1)
  1377. PCR using genomic DNA - (reply: 2)
  1378. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1379. Problem amplifying plasmid - (reply: 1)
  1380. Sybr Green I in real-time PCR reaction - (reply: 3)
  1381. Primers annealing temperatures - (reply: 8)
  1382. Difficulties cloning by PCR - (reply: 5)
  1383. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1384. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1385. high Cp value PCR - (reply: 2)
  1386. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1387. Synthesize own Oligo-dT primers - (reply: 6)
  1388. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1389. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1390. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1391. Smearing/ no amplification in PCR - (reply: 3)
  1392. primer design - Primer design for PCR (reply: 1)
  1393. How are primers designed? - (reply: 4)
  1394. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1395. DNA methylated PCR - (reply: 2)
  1396. need help on primer calcuation soon - (reply: 1)
  1397. primer design - (reply: 6)
  1398. problem in pcr and purification - (reply: 3)
  1399. Inability to duplicate PCR results - (reply: 8)
  1400. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1401. PCR using xt5 - (reply: 2)
  1402. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1403. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1404. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1405. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1406. 23S rRNA Primer - need primer sequence (reply: 1)
  1407. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1408. RT-PCR - problems with TaqMan PCR (reply: 2)
  1409. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1410. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1411. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1412. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1413. Small product amplification problems - (reply: 4)
  1414. multi way ligation or long range pcr? - (reply: 1)
  1415. Freezing primers+SYBR green - (reply: 2)
  1416. Tagged Primers - (reply: 5)
  1417. no pcr amplification product - (reply: 9)
  1418. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1419. design primer for chip - (reply: 5)
  1420. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1421. PCR problem - (reply: 4)
  1422. differently behavioring replicates in real-time PCR - (reply: 1)
  1423. Multiplex PCR - (reply: 1)
  1424. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1425. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1426. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1427. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1428. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1429. PCR components storage - (reply: 3)
  1430. reconstitution of primers - (reply: 2)
  1431. No PCR bands with some templates - (reply: 5)
  1432. Mysterious PCR Contamination - (reply: 13)
  1433. BS PCR..please help - DNA methylation analysis (reply: 15)
  1434. RT-PCR Help - Protocol provided (reply: 4)
  1435. PCR product purification - (reply: 3)
  1436. Mutagenesis PCR and undesired amplification - (reply: 2)
  1437. Temp Gradient PCR - (reply: 2)
  1438. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1439. PCR with genomic dna - (reply: 9)
  1440. primer dimer - (reply: 2)
  1441. PCR with long primers - (reply: 3)
  1442. BSP PCR standardization...help - (reply: 8)
  1443. PCR on degraded templates - (reply: 2)
  1444. PCR help needed - (reply: 3)
  1445. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1446. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1447. light bands, dark smear, dark dimers - (reply: 2)
  1448. PCR, followed by sequencing... - why ?? (reply: 3)
  1449. PCR program - strange extension step (reply: 2)
  1450. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1451. Help me! How to design nested BSP primers? - (reply: 4)
  1452. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1453. Plasmid linearization by PCR - (reply: 4)
  1454. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1455. Using BLAST to check primers - (reply: 17)
  1456. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1457. pcr doesnt show any band for DNa walking - (reply: 8)
  1458. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1459. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1460. Effects of more cDNA in PCR??? - (reply: 1)
  1461. PCR primer - (reply: 2)
  1462. PCR product disappears after restriction digestion - (reply: 4)
  1463. Primer design - GC clamp - (reply: 8)
  1464. Real Time PCR - excluding Ct - (reply: 2)
  1465. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1466. Price of a real time PCR machine? - (reply: 4)
  1467. colony PCR - (reply: 8)
  1468. probe conc. of Asymmetric PCR - (reply: 1)
  1469. primer dimer or what? - (reply: 2)
  1470. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1471. Real Time PCR method comments - (reply: 2)
  1472. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1473. colony pcr - (reply: 4)
  1474. Primers and Taqman Probes mixture question - (reply: 1)
  1475. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1476. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1477. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1478. Colony PCR - (reply: 15)
  1479. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1480. no bands in my PCR - (reply: 9)
  1481. designing primers - (reply: 1)
  1482. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1483. purefication and amplification of serum free DNA - (reply: 1)
  1484. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1485. Bisulfite Sequencing PCR - (reply: 4)
  1486. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1487. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1488. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1489. mRNA PCR - (reply: 1)
  1490. pcr product quantification - (reply: 5)
  1491. Negative Control Primers for ChIP Assay - (reply: 3)
  1492. Magnesium in PCR - (reply: 1)
  1493. PCR Sample Prep - (reply: 4)
  1494. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1495. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1496. Bizarre Contamination in PCR - (reply: 7)
  1497. PCR Cloning-large primers - (reply: 4)
  1498. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1499. forward and reverse primer - (reply: 4)
  1500. Check the primers - (reply: 4)
  1501. PCR problem from transformed TOPO TA vector - (reply: 3)
  1502. DIG DNA PCR labeling problem - (reply: 6)
  1503. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1504. BSP primers - (reply: 2)
  1505. Various size of insert after colony screen by PCR - (reply: 2)
  1506. inverse PCR molecular bilogy - (reply: 3)
  1507. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1508. failed PCR on DNA extract from blood - (reply: 6)
  1509. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1510. Primer dimer issue in real time PCR - (reply: 21)
  1511. Leaky RT-PCR - (reply: 4)
  1512. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1513. PCR, RNA, Northern Blotting???? - (reply: 1)
  1514. bizzare PCR smear - why does science hate me (reply: 7)
  1515. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1516. PCR detection of SNP - (reply: 6)
  1517. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1518. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1519. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1520. Sub-cloning sticky-end PCR products - (reply: 2)
  1521. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1522. ChIP on chip amplification problems - (reply: 11)
  1523. Genotyping PCR - (reply: 3)
  1524. PCR screening of transformed bacterial colony - (reply: 6)
  1525. Is this standard valid? - pcr product as standards (reply: 5)
  1526. primer binding - (reply: 1)
  1527. weird ChIP primers! - (reply: 1)
  1528. PCR primers - (reply: 1)
  1529. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1530. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1531. amplification in negative control in RT PCR - (reply: 2)
  1532. miRNA real time PCR by SYBR green methods - (reply: 1)
  1533. Deletion PCR - (reply: 9)
  1534. diluting to final primer concentration - please help correct (reply: 1)
  1535. Smear problem with my new primer set - (reply: 2)
  1536. PCR ghost bands - (reply: 1)
  1537. Amplifying a gene using a degenerate primer - (reply: 4)
  1538. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1539. CP of real time PCR by LC480 - (reply: 1)
  1540. CHIP- Real Time PCR calculations - (reply: 1)
  1541. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1542. transgene copy number - using real time PCR (reply: 1)
  1543. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1544. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1545. Unspecific PCR - (reply: 7)
  1546. Real-Time PCR as a Microplate Reader - (reply: 1)
  1547. gene-specific RT-PCR - (reply: 4)
  1548. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1549. RT-PCR Internal Standard - (reply: 2)
  1550. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1551. Plasmid supercoiling affecting PCR? - (reply: 2)
  1552. RNA contamination in PCR - (reply: 1)
  1553. PCR without DNA extraction! - (reply: 2)
  1554. Primer tm - (reply: 1)
  1555. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1556. re-amplification in real-time PCR - (reply: 1)
  1557. primer design - really necessary? (reply: 2)
  1558. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1559. RT-PCR - (reply: 3)
  1560. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1561. Degenerative primers = multiple products? - (reply: 5)
  1562. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1563. PCR problems on a ligation product - (reply: 5)
  1564. Genomic DNA and PCR - (reply: 4)
  1565. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1566. Sewing PCR - (reply: 3)
  1567. on the minimal pcr derived product - (reply: 2)
  1568. primer dimers - (reply: 5)
  1569. RT-PCR vs. conventional PCR - (reply: 1)
  1570. qPCR primer design - (reply: 5)
  1571. PCR and gel purification problem - did I screw this up (reply: 4)
  1572. Cloning PCR fragment - (reply: 1)
  1573. touchdown pcr - (reply: 6)
  1574. non specific pcr products - (reply: 4)
  1575. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1576. Anyone with experiences in 2 step PCR - (reply: 1)
  1577. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1578. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1579. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1580. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1581. re-amplification of a PCR product - is it recommended? (reply: 7)
  1582. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1583. PCR machine with accurate temperature control? - (reply: 1)
  1584. Gene-specific RT-PCR - (reply: 2)
  1585. Primer stock confusion - (reply: 6)
  1586. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1587. primer design - primer design question (reply: 2)
  1588. Protocol for 16S rDNA real time PCR - (reply: 2)
  1589. Touchdown PCR - (reply: 1)
  1590. Determine annealing temperature of primers - (reply: 14)
  1591. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1592. polymerase for BSP - (reply: 2)
  1593. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1594. Validating real time pcr primers - (reply: 2)
  1595. BSP,MSP primer design? - (reply: 1)
  1596. low gc primers - help with pcr using low gc primers (reply: 6)
  1597. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1598. Primers and annealing temperature - (reply: 2)
  1599. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1600. Copy number calculations in real time PCR - (reply: 2)
  1601. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1602. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1603. RT-PCR Negative controls - (reply: 1)
  1604. WHich primer to use for sequencing - (reply: 5)
  1605. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1606. minimum length for the gene to be amplified in PCR - (reply: 6)
  1607. dNTP Quantity - (reply: 3)
  1608. annoying PCR cloning problem (season 2) - (reply: 5)
  1609. PCR with 60 bp primers - results in no product (reply: 6)
  1610. Amplification dwindles using little template RT-PCR - (reply: 3)
  1611. Running gel after RT-PCR - (reply: 5)
  1612. Degenerate PCR Size Limit? - (reply: 3)
  1613. primer design for pBAD topo expression kit - (reply: 2)
  1614. PCR problems.. - problems with conventional PCR (reply: 3)
  1615. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1616. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1617. small PCR products on agarose gel? - (reply: 8)
  1618. Dnase digestion and PCR - (reply: 4)
  1619. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1620. primers fpr sequencing - (reply: 4)
  1621. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1622. Need an UV260 RT-PCR instrument - (reply: 1)
  1623. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1624. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1625. PCR produces products of wrong size - (reply: 4)
  1626. PCR with pfu and degenerate primers (?) - (reply: 7)
  1627. a doubt about PCR gel purification - (reply: 4)
  1628. Draw PCR primer locations - PCR primer (reply: 4)
  1629. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1630. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1631. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1632. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1633. PCR+enhancer - (reply: 4)
  1634. Serious issues with RT-PCR - (reply: 7)
  1635. PCR [dNTP] - dNTP concentrations (reply: 2)
  1636. PCR issue - (reply: 2)
  1637. dNTP question - (reply: 3)
  1638. PCR - (reply: 1)
  1639. long PCR primer - (reply: 7)
  1640. Taqman rtPCR primer and probe design - (reply: 3)
  1641. sequencing problem after pcr - (reply: 4)
  1642. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1643. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1644. primer design - (reply: 3)
  1645. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1646. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1647. Analysis of ChIP RT-PCR data - (reply: 7)
  1648. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1649. Negative flouroscence in real time PCR - (reply: 2)
  1650. Minimum number of PCR cycles to see a product? - (reply: 6)
  1651. decontamination - decontamination for PCR (reply: 6)
  1652. Troubleshoot ARMS PCR - (reply: 2)
  1653. negative control for PCR - (reply: 1)
  1654. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1655. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1656. pcr pdt amplification - (reply: 1)
  1657. real time PCR - (reply: 3)
  1658. PCR fails when I scale up - (reply: 2)
  1659. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1660. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1661. PCR not Working = SMEAR - (reply: 3)
  1662. How to do PCR fragment in 2 steps - (reply: 3)
  1663. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1664. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1665. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1666. RT-PCR - problem of PCR (reply: 1)
  1667. PCR product longer than template - why? - (reply: 2)
  1668. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1669. Dye recipe that can be used for PCR MM - (reply: 3)
  1670. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1671. PCR conditions with three primers - (reply: 1)
  1672. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1673. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1674. Cloning a labeled PCR product - (reply: 1)
  1675. PCR and cloning - (reply: 14)
  1676. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1677. PCR product - not suppose to be there (reply: 7)
  1678. using the meth primer - (reply: 2)
  1679. Generating primers for MSP - (reply: 1)
  1680. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1681. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1682. PCR from a smear genomic DNA ? - (reply: 5)
  1683. Primer design and need help - (reply: 11)
  1684. Setting up a multiplex PCR assay. - (reply: 2)
  1685. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1686. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1687. cDNA and RT-PCR - (reply: 8)
  1688. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1689. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1690. PCR problem - help me plsss (reply: 9)
  1691. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1692. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1693. PCR efficiencies - (reply: 4)
  1694. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1695. PCR quantification - (reply: 1)
  1696. PCR quantification - (reply: 1)
  1697. Long PCR product - (reply: 9)
  1698. problem in Pcr amplification - (reply: 7)
  1699. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1700. difference between hotstart and taq polymerase - (reply: 7)
  1701. sequencing the pcr product - (reply: 15)
  1702. Pcr amplification - primer designed from UTR regions (reply: 5)
  1703. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)