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PCR Related Discussions
  1. My PCR suddenly stopped working and I'm losing my mind - (reply: 3)
  2. Concentration of Eva green with different amplicon sizes in a PCR pool - (reply: 1)
  3. labX offers on realtime PCR machines - (reply: 4)
  4. Could any of you help with an explanation to these vague real time PCR curves &# - (reply: 1)
  5. polymerase with or without proofreading activity used in study nucleotide polymo - (reply: 1)
  6. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  7. Suggest best micropipette for PCR purpose... - (reply: 1)
  8. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  9. Plasmid Amplification Issue - (reply: 6)
  10. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  11. primer design for a gene - (reply: 2)
  12. large bands in all pcr reactions including negative control - (reply: 1)
  13. confirmation for primers dilution - (reply: 10)
  14. Problems regarding amplification of my gene - (reply: 3)
  15. PCR working, qPCR is *not*. - (reply: 6)
  16. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  17. Overlapping PCR, need help - (reply: 5)
  18. How does RT-PCR work? - (reply: 5)
  19. Denatured plasmid for pcr - (reply: 1)
  20. Possible primer dimer problem..Need solution!!! - (reply: 2)
  21. confusing with where to put primers and which ordination - (reply: 4)
  22. Help with primer concentration for sequencing - (reply: 1)
  23. PCR troubleshoot - (reply: 5)
  24. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  25. PCR Products not run well in the GEL - (reply: 1)
  26. PCR solution calculus - (reply: 4)
  27. Amplifying from pcr products - (reply: 7)
  28. Consistancy issue with PCR block - (reply: 1)
  29. Queries regarding PCR - (reply: 3)
  30. Design the primers - (reply: 10)
  31. Calculation of Taq Polymerase - (reply: 3)
  32. Best Taq for colony PCR? - (reply: 5)
  33. How two fragments joint together when doing fusion PCR? - (reply: 4)
  34. How to test my milleq water in q PCR about contaminants - (reply: 3)
  35. Mutagenesis PCR - (reply: 9)
  36. real time PCR primer design - (reply: 1)
  37. The PCR gods are frowning upon me - (reply: 9)
  38. PCR after T4 ligation? - (reply: 3)
  39. primers for cloning - (reply: 1)
  40. Degenerated Primers and their problems - (reply: 1)
  41. Sonication of small PCR amplicon - (reply: 1)
  42. primer design with restriction enzyme - (reply: 1)
  43. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  44. primer dilution help - (reply: 1)
  45. Testing primers on 'unknown' tissue - (reply: 1)
  46. How to remove inhibitory substances in PCR? - (reply: 2)
  47. need some advise about PCR of PKD1-human gene - (reply: 7)
  48. puzzled with PCR outcome after BS treatment - (reply: 2)
  49. nested PCR for low viral load- HBV patient sample - (reply: 2)
  50. troubleshooting stubborn PCR - (reply: 6)
  51. Measuring pcr fragments in a gel - (reply: 1)
  52. Amplification in HK gene but not for target gene - (reply: 3)
  53. could you help me with my stem loop RT primer? - (reply: 1)
  54. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  55. How long can I store at - 30°C my PCR mix? - (reply: 6)
  56. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  57. RT-PCR help - (reply: 2)
  58. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  59. PCR Temperature change control malfunctioning - (reply: 1)
  60. excess amount of primers - (reply: 2)
  61. Dehydrating and Reconstituting primers - (reply: 15)
  62. Trouble with PCR of short sequence - (reply: 3)
  63. Setting the temperature range for gradient PCR - (reply: 3)
  64. KIR gene promoters for MSP primers design - (reply: 2)
  65. Biotin-streptavidin signal amplification - (reply: 1)
  66. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  67. Amplification curve in the negative control samples - (reply: 7)
  68. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  69. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  70. Electrophoresis after PCR : too many bands - (reply: 6)
  71. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  72. Primer as limiting reagent in PCR reaction - (reply: 2)
  73. Primer Design for RNA probes - (reply: 2)
  74. Real time PCR for degraded RNA - (reply: 1)
  75. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  76. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  77. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  78. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  79. Nested BSP Primer Design - (reply: 6)
  80. website for primer design - (reply: 1)
  81. PCR Efficiency over 150%! - (reply: 1)
  82. problem of amplification - (reply: 2)
  83. PCR not working - (reply: 11)
  84. How to do a primer dilution - (reply: 10)
  85. Troubles with Fusion PCR - (reply: 1)
  86. Primer design and alternative transcripts - (reply: 2)
  87. PCR and sequencing of genomic DNA - (reply: 4)
  88. qPCR amplification - (reply: 4)
  89. NESTED PCR - (reply: 6)
  90. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  91. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  92. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  93. Digestion necessary after PCR? - (reply: 9)
  94. Inverse PCR product selection - (reply: 2)
  95. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  96. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  97. how to hasten real-time PCR amplifications - (reply: 2)
  98. Please please help me with my Phusion PCR. - (reply: 5)
  99. PCR - (reply: 1)
  100. NCBI Primer Design - Stringency Issues - (reply: 3)
  101. Different primer concentration in qPCR - (reply: 1)
  102. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  103. help in long pcr - (reply: 1)
  104. PCR inhibitor in template DNA - (reply: 3)
  105. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  106. Problem with Real-time PCR results analysis - (reply: 1)
  107. Primer Specificity: Testing only one primer - (reply: 4)
  108. PCR from protozoa DNA - (reply: 3)
  109. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
  110. tool for comparing many primers pairs - (reply: 4)
  111. PCR that leads to protein synthesis - (reply: 18)
  112. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  113. Use of DMSO in General PCR - (reply: 1)
  114. PCR product size confusion - (reply: 3)
  115. Pcr primers - (reply: 7)
  116. Concentration specification in PCR - (reply: 3)
  117. Guanidine isothiocyanate in PCR - (reply: 1)
  118. Primers have worked well but now getting primer dimers? - (reply: 2)
  119. I cannot design primers on exon-exon junction - (reply: 2)
  120. DNA Quantification of PCR Products - (reply: 2)
  121. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  122. Problem for PCR - (reply: 9)
  123. Designing primers in UTRs - (reply: 1)
  124. Multiplex PCR - (reply: 1)
  125. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  126. primer design@ buy? - (reply: 2)
  127. Trouble with overlap extension pcr - (reply: 3)
  128. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  129. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  130. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  131. Question about the RT PCR - (reply: 3)
  132. PCR ready mixes with long shelf lives - (reply: 4)
  133. Troubleshooting: Inverse PCR - (reply: 3)
  134. How to design primer to amplify genomic DNA? - (reply: 3)
  135. Overlap PCR, need help - (reply: 11)
  136. Internal control for miRNA RT-PCR - (reply: 1)
  137. Primers - (reply: 1)
  138. Primers mix - (reply: 2)
  139. question about RNA concentration for real time PCR - (reply: 1)
  140. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  141. PCR products sizes and DNA ladder - (reply: 7)
  142. Having problem with primers for qPCR - (reply: 4)
  143. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  144. PCR product sequencing - (reply: 3)
  145. Bad fragment amplification - (reply: 4)
  146. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  147. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  148. No amplification with TRAPEZE kit! - (reply: 1)
  149. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  150. Stargazer PCR problems - (reply: 3)
  151. General PCR discussion - (reply: 8)
  152. Real time. No amplification but hight flourescence - (reply: 1)
  153. Real time PCR doubt - (reply: 5)
  154. PCR Profile for ligation - (reply: 3)
  155. protocol to relieve melanin inhibition of PCR - (reply: 4)
  156. No bands despite different primers and conditions and TAqs - (reply: 8)
  157. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  158. Equimolar Mix Primer - (reply: 8)
  159. Wrong PCR product - (reply: 2)
  160. Colony PCR positive and Digestion negative????? - (reply: 11)
  161. Primer design - (reply: 5)
  162. storage for lyophilized primers - (reply: 1)
  163. PCR GAPDH gene - (reply: 1)
  164. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  165. Quantitative RT-PCR statistics help - (reply: 1)
  166. Dimerization of PCR product - (reply: 4)
  167. PCR Primer trouble - (reply: 2)
  168. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  169. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  170. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  171. finding the corrosponding primers - (reply: 2)
  172. Gene sequence for Real time PCR - (reply: 2)
  173. non-reproducible PCR results with cDNA as template - (reply: 1)
  174. Ligation of PCR fragments - (reply: 11)
  175. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  176. RNA extraction for RT-PCR - (reply: 3)
  177. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  178. problems with gDNA doing real time PCR in yeast - (reply: 2)
  179. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  180. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  181. How to get rid of bands in PCR negative control - (reply: 10)
  182. T7 and M13 primers two band amplification - (reply: 3)
  183. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  184. Strange PCR problem - (reply: 2)
  185. Question about Double Digestion followed by PCR amplification - (reply: 2)
  186. PCR problem - (reply: 2)
  187. PCR machine not working properly - (reply: 1)
  188. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  189. PCR gene specific amplification problem - (reply: 3)
  190. Failure SYBRGREEN PCR - (reply: 4)
  191. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  192. without RE site in PCR product - (reply: 5)
  193. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  194. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  195. real-time pcr non reproducible - (reply: 4)
  196. Strange amplification plots with high Ct variability - (reply: 1)
  197. How big a role does mixing play in PCR - (reply: 1)
  198. Melting curve is irregular for primer optimization - (reply: 5)
  199. Designing primers for ABO blood groups - (reply: 1)
  200. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  201. RT-PCR primer design - (reply: 7)
  202. How to amplify very short PCR template - (reply: 4)
  203. Is this primer okay? - (reply: 4)
  204. PCR amplification of large template - (reply: 1)
  205. PCR insert - in frame? - (reply: 2)
  206. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  207. smear in gel electrophoresis after PCR - (reply: 2)
  208. Whole plasmid amplification by PCR - (reply: 2)
  209. PCR product as standard curve template - (reply: 6)
  210. colony PCR after transformation - (reply: 1)
  211. PCR machine - (reply: 3)
  212. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  213. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  214. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  215. Resuspending primers calculation - (reply: 4)
  216. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  217. PCR RFLP is PCR product purification necessary? - (reply: 3)
  218. Mystery in my PCR - (reply: 5)
  219. Whole genome amplification from cDNA? - (reply: 4)
  220. What do you use to check primer secondary structure? - (reply: 3)
  221. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  222. PCR product as template for in vitro transcription - (reply: 1)
  223. Problem with 3 step PCR - (reply: 3)
  224. mutagenic primers with very high GC content. - (reply: 3)
  225. Scaling up PCR to get more DNA - (reply: 5)
  226. PCR to get 10kbp product - (reply: 4)
  227. site-directed mutagenesis primer Tm - (reply: 4)
  228. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  229. Random vs oligo primer in preamplification RT - (reply: 1)
  230. how does polymerase stop at the required length - (reply: 1)
  231. Chip pcr. are there inespecifics? - (reply: 1)
  232. Primer concentration - stupid question - (reply: 3)
  233. Primer Design, help i´m New - (reply: 4)
  234. No DNA after PCR product purification - (reply: 9)
  235. Sensitivity of RT-PCR and qPCR - (reply: 4)
  236. RT-qPCR primer problem - (reply: 4)
  237. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  238. real time PCR trouble - (reply: 3)
  239. removal of ethanol in PCR product purification - (reply: 5)
  240. Crazy real time PCR curve - (reply: 4)
  241. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  242. High fidelity PCR trouble shooting - (reply: 2)
  243. Designing cloning primers for DNMT - (reply: 2)
  244. Reproducible Non-Specific PCR Product - (reply: 2)
  245. Are these primer products good enough for qPCR? - (reply: 3)
  246. Can't get PCR with large overhang primers to work - (reply: 8)
  247. Primer Efficiency across runs - (reply: 1)
  248. Question about pipet tips for PCR and rtPCR - (reply: 4)
  249. UNG in PCR - (reply: 1)
  250. Cause of random samples failing PCR? - (reply: 2)
  251. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  252. pcr 10X buffer preparation - (reply: 2)
  253. how much template do i need for pcr? - (reply: 5)
  254. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  255. Problems with crossover PCR - (reply: 2)
  256. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  257. Designing PCR Primers for cloning - (reply: 17)
  258. Creating primers to add restriction sites to vector backbone - (reply: 7)
  259. PCR DNA Concentration - (reply: 1)
  260. RT-PCR product- no band - (reply: 4)
  261. Some primers dose not work - (reply: 5)
  262. Understanding RACE PCR - (reply: 1)
  263. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  264. Wierd Bands after PCR....Confused - (reply: 9)
  265. Query regarding primers for quick change mutagenesis - (reply: 3)
  266. PCR with Plasmid recovered from filter paper - (reply: 6)
  267. PCR amplified product size - (reply: 5)
  268. the storage time for primers - (reply: 9)
  269. Annealing temperature for PCR - (reply: 8)
  270. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  271. Nested PCR - (reply: 2)
  272. random primers or oligodT - (reply: 4)
  273. No amplification during RT-PCR - (reply: 4)
  274. Sequencing RT-PCR product - (reply: 3)
  275. Low yield PCR product after gel purification - (reply: 8)
  276. To design or use published primers? - (reply: 4)
  277. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  278. PCR primer usage (Clonning & cDNA) - (reply: 2)
  279. problem in cloning PCR primer design with restriction site - (reply: 4)
  280. Amplification with U primers, but not with M primers - (reply: 1)
  281. Taq polymerase - (reply: 7)
  282. His tag introduction in to gene and primer design - (reply: 2)
  283. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  284. Which High-Fidelity polymerase is better? - (reply: 2)
  285. polymerase to use for cloning - (reply: 4)
  286. Problem amplifying viral gene - (reply: 5)
  287. Adding tag using overlapping PCR - (reply: 2)
  288. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  289. Design primer from incomplete sequence... - (reply: 2)
  290. Design primer from incomplete sequence... - (reply: 2)
  291. How to perform colony PCR - (reply: 1)
  292. Primer designing for Methylation - (reply: 6)
  293. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  294. cDNA amplification problem - (reply: 4)
  295. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  296. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  297. PCR product running on agarose gel - (reply: 32)
  298. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  299. No bands in PCR after DNase treatment - (reply: 4)
  300. PCR amplification with high fidelity enzyme - (reply: 1)
  301. PCR HELP!!!! - (reply: 1)
  302. Overlap PCR problem - (reply: 5)
  303. PCR and restriction enzyme digestion - (reply: 3)
  304. Bisulfite PCR and cloning - (reply: 5)
  305. PCR product purification - (reply: 4)
  306. Overlapping sequence PCR primers - (reply: 1)
  307. restricted PCR plasmid runs slower - (reply: 2)
  308. BSP PCR primer design explained - (reply: 11)
  309. Bisulfite sequencing PCR worked - (reply: 5)
  310. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  311. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  312. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  313. doing PCR using PCR product , help plz !! - (reply: 5)
  314. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  315. good 16S univ. primer for qPCR? - (reply: 3)
  316. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  317. Amplification of CAG promoter problems - (reply: 4)
  318. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  319. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  320. I need help for designing a pair of primers for this sequence. - (reply: 1)
  321. PCR not working for 5 Aza treated DNA - (reply: 3)
  322. Differentiating mouse from human cells with PCR - (reply: 5)
  323. Differentiate between RT-PCR and PCR - (reply: 3)
  324. PCR band slightly BELOW expected length ?! - (reply: 14)
  325. long primers PCR - (reply: 4)
  326. unexpected band in PCR with plasmid - (reply: 4)
  327. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  328. DNA polymerase for GC rich template? - (reply: 4)
  329. Sequencing Primers and Plasmids - (reply: 3)
  330. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  331. DNA extraction - PCR Problem - (reply: 3)
  332. Primers to amplify Kan operon from pet28 - (reply: 2)
  333. PCR product biotinylation - (reply: 3)
  334. Tagged primers and a second PCR targetting the tags - (reply: 5)
  335. cloning pcr insert into plasmid - (reply: 2)
  336. Barcode generator for PCR primers - (reply: 2)
  337. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  338. Barcoding PCR products - (reply: 18)
  339. Biotinylated primers - (reply: 4)
  340. role of water in PCR - (reply: 3)
  341. HIV gene- PCR - (reply: 3)
  342. Real time PCR melting curve - (reply: 2)
  343. BSA in PCR reaction - (reply: 1)
  344. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  345. Cannot see insert with colony pcr - (reply: 6)
  346. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  347. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  348. How to Join two PCR products - (reply: 3)
  349. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  350. What's wrong with my PCR - (reply: 5)
  351. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  352. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  353. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  354. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  355. Cp, but no amplification - (reply: 2)
  356. pcr product digestion problem - (reply: 4)
  357. Low PCR product - (reply: 7)
  358. Control primers for bisulfite-converted DNA - (reply: 3)
  359. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  360. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  361. wrong dntp concentration - (reply: 2)
  362. Third round PCR - (reply: 2)
  363. long fragment PCR - (reply: 2)
  364. Best proof-reading polymerase? - (reply: 12)
  365. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  366. Q regarding primer mix for MSP!! - (reply: 4)
  367. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  368. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  369. I need to break this cycle of PCR issues - (reply: 13)
  370. Questions / Help with Fusion PCR - (reply: 8)
  371. Controls for RT-PCR reactions - (reply: 2)
  372. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  373. BSP Primers with M13 Tails - (reply: 1)
  374. Detection of T7 RNA polymerase by WB - (reply: 6)
  375. Multiplex PCR - (reply: 33)
  376. issues when designing PCR primers using PRIMER3 - (reply: 8)
  377. Primer catalog/databasing - (reply: 1)
  378. Nonrepetitive nested PCR - (reply: 2)
  379. Primer Blast - (reply: 6)
  380. Help designing primers for use in In-fushion cloning - (reply: 4)
  381. PCR internal control - (reply: 2)
  382. Primers Freeze-Thaw - (reply: 16)
  383. TDNA Genotyping PCR program - (reply: 1)
  384. primer design using 16s rRNA - (reply: 1)
  385. question about PCR and cloning - (reply: 1)
  386. PCR - (reply: 2)
  387. nonspecific band at 50bp in PCR - (reply: 9)
  388. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  389. PCR master mix - (reply: 7)
  390. Maximum PCR product Tm - (reply: 6)
  391. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  392. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  393. PCR & Southern blotting - (reply: 1)
  394. Bisulfite PCR Question - (reply: 2)
  395. Primer Design HELP! - (reply: 3)
  396. Help with sequencing of a 120bp PCR product. - (reply: 9)
  397. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  398. How are Primers made? - (reply: 9)
  399. Long Non-Specific PCR Products - (reply: 5)
  400. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  401. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  402. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  403. PCR calculation - (reply: 1)
  404. Colony PCR and digestion with KpnI - (reply: 10)
  405. designing methylation specific primers - (reply: 3)
  406. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  407. PCR - consistent false positive results - (reply: 6)
  408. PCR no amplification - (reply: 15)
  409. How to quantify virus by PCR - (reply: 12)
  410. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  411. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  412. Pcr product size determination for a fusion gene - (reply: 8)
  413. Quantify DNA before ChIP PCR - (reply: 3)
  414. Primer Annealing Temperatures - (reply: 24)
  415. my pcr product is larger than expected - (reply: 4)
  416. Question about PCR - DNA or RNA as template - (reply: 4)
  417. pcr purification - (reply: 1)
  418. pcr purification doubt - (reply: 4)
  419. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  420. PCR band moves lower than its size on agarose gel - (reply: 3)
  421. same primers, different product in conventional PCR and qPCR - (reply: 6)
  422. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  423. pcr problem - (reply: 6)
  424. NEB Q5 polymerase works very well - (reply: 1)
  425. Primers amplify genomic DNA but not cDNA - (reply: 2)
  426. pcr purification - (reply: 1)
  427. storing PCR product overnight - (reply: 2)
  428. Primer BLAST - (reply: 1)
  429. Bands in negative control PCR - (reply: 2)
  430. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  431. problem with pcr mutagenesis - (reply: 10)
  432. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  433. Use PCR purification kit to purify restriction digestion - (reply: 2)
  434. PCR primer design - published primers trustable? - (reply: 8)
  435. Polymerase for E. coli screenings - (reply: 5)
  436. complicated RT-PCR issues - (reply: 4)
  437. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  438. Can we use degenerate bases in BSP primers? - (reply: 1)
  439. Quick change mutagenesis No PCR product visible - (reply: 2)
  440. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  441. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  442. Can anyone help with my Bisulfite PCR? - (reply: 2)
  443. How to check primer sequences using BLAST and other tools? - (reply: 2)
  444. real time PCR analysis in patient samples - (reply: 2)
  445. carpet in gels for PCR products - (reply: 2)
  446. Primer Check? - (reply: 2)
  447. Sequencing of the PCR production in BSP - (reply: 1)
  448. amplification of 14Kb - (reply: 1)
  449. Validation of PCR primers/ probes - (reply: 6)
  450. Taq polymerase for MSP - (reply: 1)
  451. Real time PCR sudenly not working - (reply: 1)
  452. Buffer-composition One-Step RT-PCR - (reply: 4)
  453. Two reverse primer sequences for a single forward primer - (reply: 1)
  454. PCR with more than one primer - (reply: 1)
  455. RAPD - PCR problem - (reply: 5)
  456. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  457. How to best store PCR product? - (reply: 2)
  458. RT-PCR primer design - (reply: 1)
  459. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  460. Odd gel run following PCR - (reply: 28)
  461. Qiagen PCR Array Reagents? - (reply: 1)
  462. No or very weak band using 16s primers - (reply: 1)
  463. No PCR amplification with b-actin primers - (reply: 1)
  464. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  465. Site-directed, PCR works but no colonies - (reply: 2)
  466. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  467. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  468. No PCR amplified with long primers - (reply: 10)
  469. PCR optimization: PCR vs qPCR - (reply: 4)
  470. should I do blunt end my pcr product before ligation - (reply: 2)
  471. PCR smear for genomic samples - (reply: 4)
  472. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  473. another primer dilution question - (reply: 2)
  474. i need help with virus pcr - (reply: 2)
  475. Primer Efficiency - (reply: 2)
  476. Experimental set up for qRT PCR - (reply: 13)
  477. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  478. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  479. How should I optimize my PCR - (reply: 2)
  480. Second round of PCR after gel extraction fails miserably - (reply: 3)
  481. an effective way to do a yeast colony pcr - (reply: 2)
  482. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  483. urgent help plz with RT-PCR - (reply: 3)
  484. oligo(dt) 15 vs random primers - (reply: 3)
  485. Help to optain a long PCR produc - (reply: 3)
  486. Multiple bands in eluted PCR product - (reply: 1)
  487. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  488. Problems with two step RT-PCR - (reply: 2)
  489. Getting genomic DNA for PCR - (reply: 4)
  490. primer dilutions - (reply: 4)
  491. Genotyping PCR primers - (reply: 1)
  492. Conventional PCR - (reply: 1)
  493. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  494. PCR product loss on cleanup - (reply: 2)
  495. MSP Primers not working - (reply: 6)
  496. Primers too dilute - (reply: 1)
  497. Amplification curves jagged - (reply: 1)
  498. Cloning HELP- Possible Primer issues - (reply: 6)
  499. No PCR product in site-directed mutagenesis - (reply: 4)
  500. Giardia/Crypto real time PCR late amplification - (reply: 1)
  501. How to dilute the template DNA for PCR - (reply: 7)
  502. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  503. Need help for PCR - (reply: 5)
  504. PCR primer pairs - university exam - (reply: 7)
  505. unespecific band PCR from mouse genomic DNA - (reply: 9)
  506. Help with primer design through PRIMER3 - (reply: 4)
  507. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  508. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  509. Plasmid vs cDNA amplification by PCR - (reply: 2)
  510. Tm of primers - prediction and verification - (reply: 1)
  511. PCR cloning...help! - (reply: 5)
  512. RT-PCR contamination in negative control. - (reply: 1)
  513. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  514. how to design primers for 16sr RNA - (reply: 24)
  515. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  516. Help with primer design - (reply: 3)
  517. Primer Tm calculation. - (reply: 1)
  518. low concentration of purified PCR product - (reply: 2)
  519. PCR smearing and...problems! - (reply: 7)
  520. Running Primers on Agarose Gel - (reply: 6)
  521. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  522. universal primers for PCR ribotyping - (reply: 6)
  523. will the template of PCR be added A ? - (reply: 2)
  524. Colony PCR not working - (reply: 4)
  525. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  526. Primer design: Tm above or below Ta? - (reply: 5)
  527. Oligo primers for shRNA construction - (reply: 1)
  528. Design primers for expression cloning - (reply: 11)
  529. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  530. primer dilution query - (reply: 2)
  531. Real Time PCR polymerase enzyme - (reply: 3)
  532. Estimating size of PCR products in a agarose gel - (reply: 4)
  533. Sequencing of PCR product - (reply: 4)
  534. PCR contamination problem - (reply: 2)
  535. Using BLAST to check primers - (reply: 1)
  536. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  537. What is the function of a third primer in a PCR mix?? - (reply: 2)
  538. How can we design a primer ? step by step method - (reply: 4)
  539. cDNA synthesis or RT-PCR - (reply: 3)
  540. PCR on cDNA and SGamplification??? - (reply: 1)
  541. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  542. real time PCR protocol - controls? - (reply: 2)
  543. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  544. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  545. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  546. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  547. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  548. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  549. PCR and UV spectrophotometry - (reply: 3)
  550. RT-PCR in a single tube - (reply: 3)
  551. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  552. annealing temperature for 3 primer pairs? - (reply: 4)
  553. PCR Reaction - (reply: 3)
  554. Impact of primer sequence on TA cloning - (reply: 3)
  555. problems with Bisulfite PCR - (reply: 1)
  556. low pcr product - (reply: 3)
  557. overlapping PCR protocol - (reply: 12)
  558. PCR to amplify my insert not working - (reply: 6)
  559. Problem with PCR amplification- high 3' stability - (reply: 3)
  560. Home made real-time PCR problem - (reply: 4)
  561. High RNase concentration interfering with PCR? - (reply: 8)
  562. Pcr primers - (reply: 2)
  563. PCR failure from yeast genomic DNA - (reply: 1)
  564. failure of PCR from yeast genomic DNA - (reply: 1)
  565. RT-Q PCR primer design question - (reply: 2)
  566. high MW dimers - (reply: 2)
  567. Strange RT-PCR amplification plots - (reply: 9)
  568. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  569. What products will I get from this pcr? - (reply: 1)
  570. PCR buffer without Tris - (reply: 1)
  571. PCR Water - (reply: 4)
  572. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  573. Which Brand of real-time PCR machine - (reply: 6)
  574. Problem with PCR - large extra bands - (reply: 3)
  575. big differnece in my primers Tm - (reply: 2)
  576. Designing primers for the ORFs - (reply: 9)
  577. Two-Step PCR - (reply: 2)
  578. Gradient PCR - (reply: 2)
  579. PCR with phophorylated primers - (reply: 1)
  580. Problem with New qPCR primers - help! - (reply: 6)
  581. Designing primers for miRNA located on minus strand. - (reply: 2)
  582. Plasmid sequencing without primers - (reply: 1)
  583. Smallest insert in PCR - (reply: 6)
  584. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  585. loop-mediated amplification - (reply: 2)
  586. Blunt end PCR product ligation - (reply: 1)
  587. Help with high Ct values for Real-Time PCR - (reply: 1)
  588. Genotyping by allele specific PCR - (reply: 6)
  589. RT-PCR primers and the poly(A) tail - (reply: 2)
  590. NO Insert found in the Colony PCR - (reply: 15)
  591. No band in PCR - (reply: 5)
  592. overlapping primer - (reply: 1)
  593. PCR primers help - (reply: 5)
  594. PCR smear after one-month storage - (reply: 2)
  595. Primer dimers - (reply: 2)
  596. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  597. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  598. Simple question of dNTP's for PCR - (reply: 5)
  599. Need help for PCR for AT rich gene - (reply: 3)
  600. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  601. Primer validation and PCR efficiency - (reply: 4)
  602. How to calculate the price of PCR test per sample? - (reply: 6)
  603. Contamination in negative control of PCR (No template control) - (reply: 6)
  604. cloning with nested pcr - (reply: 3)
  605. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  606. PCR is amplifying non-specific fragment - (reply: 6)
  607. ChIP amplification problems - (reply: 1)
  608. real time RT-PCR problems - (reply: 2)
  609. should I design primers spanning both intron and exon - (reply: 2)
  610. RT-PCR - (reply: 1)
  611. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  612. Template DNA (plasmid) concentration for PCR - (reply: 4)
  613. PCR with a very long and a short primer - (reply: 5)
  614. Ligation of 16kb PCR product - (reply: 5)
  615. Multiplex PCR does not work - (reply: 3)
  616. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  617. Problem with genomic PCR (2.4 kb) - (reply: 1)
  618. Comparison between two primers pairs? Is it possible? - (reply: 3)
  619. Rt-PCR primer design - (reply: 7)
  620. Length of non-binding sequence in primers with RE site - (reply: 6)
  621. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  622. Getting a smaller PCR product than required - (reply: 6)
  623. Difficult sequences in PCR - (reply: 2)
  624. why is real time pcr curve like this? - (reply: 3)
  625. PCR inconsistency - (reply: 2)
  626. Orientation of primers - (reply: 2)
  627. PCR, neg control & no. of cycles - (reply: 2)
  628. Strange PCR result from genomic DNA - (reply: 3)
  629. Bright bands of PCR products stick in gel wells - (reply: 1)
  630. methylation analysis with MS-HRM (primer design) - (reply: 1)
  631. whole plasmid pcr? - (reply: 6)
  632. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  633. salts in PCR - (reply: 4)
  634. Strange amplification plot - (reply: 4)
  635. Conventional PCR of different dilutions - (reply: 1)
  636. primer resuspension - (reply: 2)
  637. Principles of overlap PCR - (reply: 7)
  638. ligation of pcr product into expression vector - (reply: 4)
  639. Unable to PCR!!!! - (reply: 3)
  640. Help designing primers with restriction sites - (reply: 3)
  641. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  642. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  643. Abnormal amplification curve - (reply: 1)
  644. Problem with Semiquantitative PCR - (reply: 4)
  645. Advice on optimising bisulfite PCR sought - (reply: 2)
  646. Help: I am loosing the DNA during PCR purification - (reply: 7)
  647. PCR primer with no extra DNA end - (reply: 5)
  648. PCR colonies of Pichia pastoris - (reply: 1)
  649. ROX reference dye for quantitative RT-PCR - (reply: 1)
  650. PCR problem - (reply: 9)
  651. why PCR product smeared? - (reply: 2)
  652. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  653. silincing box, no amplification - (reply: 3)
  654. Comparative semiquantitative RT-PCR - (reply: 2)
  655. Bands in my PCR Controls - (reply: 3)
  656. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  657. Strange band in colony PCR - (reply: 2)
  658. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  659. Multiplex PCR - (reply: 12)
  660. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  661. PYO pcr stopped working. - (reply: 1)
  662. Bands seen with qPCR missing in regular PCR - (reply: 5)
  663. checking msp primers - (reply: 2)
  664. PCR product one day, none the next day - (reply: 4)
  665. PCR on colony - (reply: 10)
  666. PCR reaction ISSUES???? need help - (reply: 4)
  667. PCR using CDNA as template - (reply: 2)
  668. RT-PCR doesn't work with all RNA used - (reply: 1)
  669. Need help amplifying repeating sequence. - (reply: 5)
  670. Real Time PCR 101...help! - (reply: 1)
  671. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  672. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  673. Another primer dimers problem - (reply: 23)
  674. Colony PCR Problem!!!! - (reply: 5)
  675. using PCR product as standard template - (reply: 3)
  676. PCR kit - (reply: 7)
  677. Make additional cycles with an already finished pcr product - (reply: 3)
  678. Amplification in water control but not in samples - (reply: 3)
  679. PCR products form with DNA, but not with c-DNA - (reply: 2)
  680. question about pcr amplification efficiency? - (reply: 1)
  681. DNA polymerase: recombinant or native? - (reply: 3)
  682. pcr clean-up fail? - (reply: 1)
  683. MSP U primers very difficult to amplify... - (reply: 4)
  684. No gene amplification, 18s amplification is fine - (reply: 1)
  685. ChIP-qPCR gives odd amplification plot - (reply: 5)
  686. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  687. sequential cloning of multiple PCR products - how to do? (reply: 3)
  688. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  689. Primer design - (reply: 3)
  690. Tomato Actin PCR+Images included - (reply: 2)
  691. single primer PCR - (reply: 5)
  692. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  693. cDNA synthesis + amplification - (reply: 2)
  694. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  695. LacZ PCR Problems - (reply: 1)
  696. cells stably expressing T7 RNA polymerase - (reply: 1)
  697. Primer concentration - (reply: 4)
  698. nested PCR with low target DNA? - (reply: 2)
  699. RT-PCR calibrator - (reply: 2)
  700. RNA - extraction and pcr (reply: 1)
  701. problem in pcr - (reply: 2)
  702. Can someone recommend me a virtual PCR software? - (reply: 6)
  703. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  704. Primer 3' mismatch - Strange experience (reply: 9)
  705. real-time PCR after restriction enzyme digestion - (reply: 6)
  706. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  707. Adding loading/tracking dye to PCR mix? - (reply: 10)
  708. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  709. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  710. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  711. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  712. how to get rid of non specific bands in PCR - (reply: 1)
  713. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  714. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  715. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  716. PCR cloning problem - could not get colony (reply: 4)
  717. pcr contamination - (reply: 4)
  718. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  719. Large Non-specfic bands in PCR - (reply: 3)
  720. PCR clonning - (reply: 3)
  721. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  722. 2 bands in blue colony PCR - (reply: 5)
  723. Primer dilution --> problem.. - (reply: 16)
  724. Help! Smear in Real-Time PCR Product - (reply: 1)
  725. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  726. Sybr green RT-qPCR primer - (reply: 1)
  727. Sybr green RT-qPCR primer - (reply: 1)
  728. primer design - microalgae (reply: 2)
  729. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  730. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  731. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  732. PCR yield in ng/ul - (reply: 1)
  733. No Amplification in PCR - (reply: 4)
  734. Real time PCR and percentage loss - (reply: 1)
  735. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  736. Problem with smear in PCR with certain templates - (reply: 4)
  737. Real time PCR in the presence of heme - (reply: 3)
  738. issues in PCR amplification - (reply: 2)
  739. Problems with PCR in general - (reply: 1)
  740. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  741. how to overcome primer contamination - need protocol (reply: 11)
  742. RT-PCR primer design - Primer design and evaluation (reply: 7)
  743. CHIP analysis by PCR - (reply: 4)
  744. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  745. Primer,probes dilution - (reply: 3)
  746. Splice varient quantification in RT real time PCR? - (reply: 1)
  747. negative control for msp pcr - (reply: 2)
  748. Calculating geometric mean for real-time PCR - (reply: 5)
  749. template transfer? - how does the rna polymerase switches template? (reply: 1)
  750. PCR Gel nonspecific band - (reply: 13)
  751. semiquantitative pcr - (reply: 1)
  752. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  753. PCR: Cloning Primers - (reply: 8)
  754. PCR product - (reply: 5)
  755. pcr amplify an insert in plasmid? - (reply: 2)
  756. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  757. Exon Spanning PCR Primers - (reply: 1)
  758. Colony PCR - (reply: 1)
  759. PCR to confirm double crossover - (reply: 1)
  760. Primer sequences - (reply: 1)
  761. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  762. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  763. PCR Primers - (reply: 2)
  764. Cleaning up RT-PCR product before sequencing - (reply: 1)
  765. Template Amount for PCR Amplification - (reply: 6)
  766. How to blast methylation specific and unspecific primers - (reply: 1)
  767. RT-PCR cDNA synthesis - (reply: 6)
  768. problems amplifying from cDNA - (reply: 4)
  769. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  770. No DNA after PCR purification with QiaQuick? - (reply: 4)
  771. restriction digestion against colony PCR - (reply: 1)
  772. How to check primers are of correct sequence or not? - (reply: 2)
  773. Primers... - (reply: 1)
  774. When are primer dimers a problem? - (reply: 1)
  775. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  776. PCR: always got band in the negative control - (reply: 8)
  777. primer with higher melting temperature - (reply: 2)
  778. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  779. Problems designing primers for BSP - (reply: 2)
  780. qPCR primer design - possible off-target (reply: 1)
  781. Primer dimer formation and real-time PCR - (reply: 7)
  782. Identify PCR products - (reply: 3)
  783. PCR genomic DNA using cell lysate - (reply: 1)
  784. Maximum size of overhangs in PCR - (reply: 6)
  785. Colony PCR doesn't work anymore - (reply: 5)
  786. RT-PCR t-test, and ANOVA - (reply: 1)
  787. Primer Design Urgent! - (reply: 1)
  788. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  789. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  790. PCR of microRNA first strand cDNA - (reply: 1)
  791. how to know my primer's sense ??? - help ! (reply: 2)
  792. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  793. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  794. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  795. intron spanning primers for non model organisms - (reply: 2)
  796. Primer design - (reply: 2)
  797. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  798. qPCR amplification interference fixed with freezing - (reply: 6)
  799. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  800. software for quantifing PCR product band - (reply: 1)
  801. Multiple bands from purified PCR product - (reply: 3)
  802. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  803. Doing PCR on Nebulized DNA - (reply: 2)
  804. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  805. RT-PCR - (reply: 9)
  806. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  807. inverse pcr - (reply: 2)
  808. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  809. Primer seq - (reply: 2)
  810. Urgent: should you dry PCR product - (reply: 13)
  811. PCR product size - (reply: 6)
  812. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  813. oligo dt and random primer - (reply: 3)
  814. mRNA Search for RT-PCR (U to T) - (reply: 2)
  815. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  816. PCR Mastermix - (reply: 4)
  817. PCR triplicates versus one reaction - differences? (reply: 17)
  818. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  819. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  820. Primers and self and hetero dimers - (reply: 1)
  821. Really weird amplification curves - (reply: 1)
  822. Primers for qPCR - (reply: 3)
  823. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  824. RT-PCR primer - (reply: 3)
  825. PCR problem - basic PCR for plasmid amplification (reply: 2)
  826. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  827. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  828. Unpredictablity of PCR product - (reply: 5)
  829. total cDNA amplification by PCR - (reply: 2)
  830. Detection limit of a conventional PCR - (reply: 2)
  831. PCR for MULTIPLE mutagenesis - (reply: 4)
  832. Using Sybr green in realtime pcr - (reply: 1)
  833. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  834. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  835. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  836. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  837. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  838. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  839. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  840. Digested PCR product migrate slower than uncut - (reply: 8)
  841. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  842. PCR question - (reply: 2)
  843. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  844. 2 rounds PCR got problem - (reply: 2)
  845. PCR help minus strand - Primer design (reply: 1)
  846. chloramphenicol storage and amplification - (reply: 3)
  847. Smear in long distance PCR - (reply: 39)
  848. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  849. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  850. TOUCH DOWN PCR - (reply: 2)
  851. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  852. PCR stopped working - After changing buffer (reply: 5)
  853. ChIP PCR question - (reply: 33)
  854. PCR protocol questions! - (reply: 2)
  855. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  856. DNA amount calculation for PCR - (reply: 14)
  857. ChIP primer design - (reply: 1)
  858. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  859. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  860. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  861. How to analyze ChIP PCR data - (reply: 10)
  862. Questions regarding RT-PCR optimization - (reply: 2)
  863. number of copies after pcr? - (reply: 1)
  864. PCR double-bands - (reply: 1)
  865. Problems with semi-quatitative PCR - (reply: 1)
  866. Question about Primers - (reply: 3)
  867. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  868. Large fragment amplification failed - (reply: 3)
  869. purification of PCR product for cloning in a vector - (reply: 3)
  870. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  871. PCR reaction calculation - (reply: 2)
  872. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  873. Primer design with a tag - (reply: 4)
  874. no increase in fluorescence in my real time PCR - (reply: 3)
  875. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  876. Help with real-time PCR quantification of miRNA - (reply: 8)
  877. PCR, using gel extracted band as a template - (reply: 4)
  878. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  879. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  880. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  881. Combining forward and reverse primer gives different size on gel - (reply: 2)
  882. Q-PCR and RT-PCR - (reply: 3)
  883. calculating total amount - in a pcr reaction (reply: 4)
  884. Problems with amplifying microRNA - (reply: 3)
  885. PCR of complement genomic DNA - (reply: 3)
  886. primers for fungal identification - (reply: 9)
  887. confusing PCR - (reply: 1)
  888. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  889. PCR for long and repetitive region from genomic DNA - (reply: 4)
  890. difference between PCR primers and sequencing primers - (reply: 1)
  891. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  892. PCR of repeated region - (reply: 1)
  893. Sense and antisense DNA, and primer design - (reply: 6)
  894. PCR bands in NTC but NOT in negative samples - (reply: 2)
  895. housekeeping genes in PCR - (reply: 1)
  896. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  897. Question about RealTime PCR Primer Design - (reply: 1)
  898. Designing a Primer with a RS that has a W - (reply: 1)
  899. Primers stopped working!? - PCR Primers (reply: 6)
  900. NFK beta primers - (reply: 4)
  901. final PCR product - need clarifications (reply: 1)
  902. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  903. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  904. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  905. RT-PCR result !! - (reply: 4)
  906. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  907. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  908. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  909. why genomic can't be use for pcr? - (reply: 2)
  910. Primer design - Primer design (reply: 2)
  911. Re-use unamplified PCR product - (reply: 2)
  912. Reason for odd PCR conditions - (reply: 3)
  913. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  914. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  915. pcr - problem in pcr (reply: 2)
  916. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  917. How to present and analyze these real-time PCR data? - (reply: 2)
  918. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  919. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  920. PCR Master mix - (reply: 2)
  921. cDNA storage and real time RT PCR - (reply: 3)
  922. Real Time PCR Primers and Probes - (reply: 2)
  923. Anyone make their own PCR cloning vector? - (reply: 5)
  924. Stability of PCR T-overhangs - (reply: 1)
  925. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  926. PCR product wrong size! Need help! - (reply: 9)
  927. pooling different pcr cycles? - (reply: 1)
  928. Amplification of concatenated linear ligated fragments - (reply: 4)
  929. Clinical Real-time PCR assay - (reply: 1)
  930. Need help - How we can design primer for miRNA (reply: 1)
  931. primer and promoter sequences - (reply: 1)
  932. Standard curve for real-time PCR - (reply: 2)
  933. designing of primers - (reply: 2)
  934. Primer Tm differences/ PCR - (reply: 1)
  935. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  936. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  937. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  938. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  939. Designing primer to remove his-tag - (reply: 1)
  940. PCR Primer Dilution from F+R - (reply: 1)
  941. primer reconstitution - primer reconstitution (reply: 4)
  942. Not-Quite-Nested PCR - (reply: 2)
  943. PCR product too short - (reply: 6)
  944. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  945. Primer design for insert amplification - Is this correct? (reply: 2)
  946. got smear on the PCR gel !!! - (reply: 4)
  947. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  948. "Unzipped" PCR band - (reply: 3)
  949. Review on new amplification techniques - (reply: 2)
  950. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  951. DOT BLOT for pcr product - No result obtain (reply: 1)
  952. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  953. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  954. HRM primers - (reply: 1)
  955. PCR amplification with Pfu / quality of DNA - (reply: 4)
  956. primer design - (reply: 2)
  957. Is it possible to compare data between real-time PCR plates - (reply: 1)
  958. Conventional PCR problems - wisamni2004 (reply: 2)
  959. New to sequencing, primer design - (reply: 2)
  960. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  961. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  962. incorrect/smaller band size for real time PCR - (reply: 1)
  963. Quickchange mutagenesis primer - (reply: 3)
  964. pcr / primer theory in bisulfite sequencing - (reply: 4)
  965. homologous amplicon for real time pcr - (reply: 1)
  966. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  967. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  968. assistance with PCR amplification please - (reply: 3)
  969. I need an urgent help with q-pcr amplification plots - (reply: 11)
  970. Problems with the Specificity of the Primers - (reply: 2)
  971. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  972. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  973. Degenerate primers PCR problem, Please help! - (reply: 2)
  974. Hotstart PCR and unspecific Amplification - (reply: 3)
  975. Primer design help needed - (reply: 1)
  976. Primers no longer work - (reply: 1)
  977. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  978. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  979. RT PCR for known snps detection? - SNPs detection (reply: 1)
  980. colony PCR - (reply: 2)
  981. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  982. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  983. PCR reaction without template gives a product - (reply: 6)
  984. PCR wrong product size - (reply: 6)
  985. Wrong pcr product size - (reply: 4)
  986. real time PCR machine - (reply: 5)
  987. PCR very faint product - (reply: 1)
  988. Question about principle of PCR - (reply: 3)
  989. PCR amplification before bisulfite conversion - (reply: 6)
  990. 4 question for real time PCR - (reply: 5)
  991. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  992. PCR didn't work - suspect the enzyme (reply: 2)
  993. Find universal primers in vector - any online tool? (reply: 6)
  994. Picking primers to confirm Illumina meth27 results - (reply: 2)
  995. Colony PCR Specificity - (reply: 2)
  996. Colony Pcr - primers - (reply: 5)
  997. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  998. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  999. Primer Dilution Problem - D'oh! (reply: 2)
  1000. Using PCR to create overhangs - (reply: 3)
  1001. Creating overhangs with PCR - (reply: 4)
  1002. Problem with PCR on bacmids - (reply: 1)
  1003. Is there any software that can be sued to design degenerate primers - (reply: 1)
  1004. Running real time pcr product on gel?.. - (reply: 4)
  1005. Primer Decontamination - (reply: 2)
  1006. PCR mutagenesis of plasmid - (reply: 5)
  1007. tools for checking non-specific binding of primers -
    (reply: 1)
  1008. Primer designed for ARMS PCR - (reply: 2)
  1009. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  1010. methylation specific PCR - Primers - (reply: 1)
  1011. bacterial identification using real time PCR - (reply: 1)
  1012. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1013. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1014. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1015. colony PCR for subcloning gene - (reply: 1)
  1016. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1017. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1018. PCR reamplification - (reply: 2)
  1019. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1020. How many colonies to screen (colony PCR)? - (reply: 4)
  1021. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1022. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1023. question about pcr - (reply: 3)
  1024. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1025. How to construct a standard curve for real time PCR - (reply: 1)
  1026. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1027. PCR with one primer - (reply: 1)
  1028. colony PCR inconsistent - (reply: 4)
  1029. PCR contamination - (reply: 19)
  1030. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1031. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1032. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1033. PCR not working - No amplification - (reply: 5)
  1034. Quantification of PCR products - (reply: 1)
  1035. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1036. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1037. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1038. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1039. Nested PCR question - (reply: 1)
  1040. High Tm on primers - cannot get a product - (reply: 8)
  1041. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1042. undesired products in multiplex PCR - (reply: 3)
  1043. NCBI primer blast problem - (reply: 1)
  1044. Help with Multiplex Nested PCR - (reply: 2)
  1045. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1046. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1047. primer sequence problem - (reply: 2)
  1048. Finding bisulfite PCR primer sequence - (reply: 3)
  1049. PCR -No band formation - (reply: 1)
  1050. PCR - No Band formation - (reply: 3)
  1051. No amplification after bisulfite treatment - (reply: 4)
  1052. PCR AMPLIFICATION - (reply: 1)
  1053. Ligation of Blunt PCR Product - (reply: 1)
  1054. real time pcr need advise - (reply: 2)
  1055. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1056. PCR & Cloning - (reply: 6)
  1057. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1058. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1059. DNA pooling for PCR - Saving money PCR (reply: 7)
  1060. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1061. PCR need some help - (reply: 4)
  1062. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1063. Left polymerase out overnight - Will it still work? (reply: 1)
  1064. Primer Reconstitution--does temperature matter? - (reply: 5)
  1065. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1066. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1067. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1068. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1069. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1070. no PCR product from input... - (reply: 8)
  1071. MSP - unwanted amplification - (reply: 5)
  1072. Smears in Bisulfite seq. PCR - (reply: 3)
  1073. Primer design for qPCR - (reply: 2)
  1074. Primer design for qPCR - (reply: 1)
  1075. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1076. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1077. Multiple bands after bisulfite PCR - (reply: 7)
  1078. PCR genomic DNA of high GC content - (reply: 6)
  1079. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1080. primer design tips - (reply: 3)
  1081. PCR with Biotin Incorporation - (reply: 2)
  1082. pcr product - hello all (reply: 1)
  1083. design pcr primer - (reply: 1)
  1084. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1085. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1086. PCR with long and complex primers - (reply: 2)
  1087. Phospholylation of primers - (reply: 1)
  1088. apoptosis by PCR? - (reply: 12)
  1089. Re-using PCR plates? - (reply: 2)
  1090. Touchdown PCR issues - (reply: 3)
  1091. LINE1 OFR2 Primer Design - (reply: 1)
  1092. phosphorylation of primers using PNK - (reply: 1)
  1093. RT-PCR contamination issue - (reply: 3)
  1094. Primers for yeast (18S) - (reply: 1)
  1095. How to reduce the bisulfite PCR bias? - (reply: 4)
  1096. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1097. Primer Concentration to lower Ct - (reply: 1)
  1098. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1099. Primer Concentration Help - (reply: 4)
  1100. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1101. Primer Degradation - What exactly happens? (reply: 1)
  1102. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1103. PCR/DNA Extraction Problem - (reply: 5)
  1104. Primers in 2 exons - (reply: 2)
  1105. NTC appear in real time pcr - (reply: 6)
  1106. Problems with designing a primer - (reply: 2)
  1107. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1108. PCR of AT-rich DNA - (reply: 1)
  1109. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1110. Overlapping PCR - really need help ! - (reply: 5)
  1111. Freezing PCR product??? - (reply: 1)
  1112. problem with pcr cloning from mouse cDNA - (reply: 2)
  1113. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1114. PCR with Platinum Taq - product yield issues - (reply: 3)
  1115. real time PCR - (reply: 7)
  1116. Different sized product for same BS primer! - (reply: 2)
  1117. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1118. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1119. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1120. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1121. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1122. primers with restriction site - (reply: 1)
  1123. PCR product appear two close band in my gel - (reply: 15)
  1124. The Case of a Missing Band: PCR Issue - (reply: 4)
  1125. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1126. cDNA as PCR template - (reply: 1)
  1127. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1128. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1129. paranormal qpcr amplification activity - melting curves (reply: 3)
  1130. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1131. PCR template concentration - (reply: 6)
  1132. Bands in PCR negative control - (reply: 3)
  1133. PCR efficiency calculated by Linreg - (reply: 3)
  1134. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1135. primer design by generunner DG - (reply: 1)
  1136. degenerate pcr - (reply: 1)
  1137. BSP primers design, help please - (reply: 2)
  1138. primers with fluoresent dye PCR cycle question - (reply: 4)
  1139. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1140. Smears on PCR products - (reply: 4)
  1141. Dumb Reverse Transcription PCR Question - (reply: 2)
  1142. Reverse transcription PCR - (reply: 1)
  1143. PCR/RT-PCR beads - (reply: 2)
  1144. High Amplification Efficiency in Std. Curve - (reply: 5)
  1145. Non specific products in Bisulphite pcr - (reply: 4)
  1146. Run PCR amplifications in agarose gels - (reply: 3)
  1147. Problems with validating primers and low expression genes - (reply: 3)
  1148. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1149. Bisulfite sequencing PCR not working - (reply: 5)
  1150. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1151. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1152. intron/exon spanning primers - (reply: 1)
  1153. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1154. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1155. PCR product for sequencing - sample storage?? (reply: 2)
  1156. Absolute quantification using Real Time PCR - (reply: 2)
  1157. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1158. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1159. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1160. MSP primer troubles - (reply: 3)
  1161. Measuring global methylation using real time PCR - (reply: 2)
  1162. Designing Primers for multiple Isoforms - (reply: 1)
  1163. RT-PCR standard curve dilutions - (reply: 2)
  1164. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1165. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1166. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1167. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1168. PCR product dimer issue - (reply: 24)
  1169. PCR additives Formamide - Formamide which one? (reply: 4)
  1170. PCR Master mix - (reply: 1)
  1171. Addition of Restriction sites into PCR primers - (reply: 4)
  1172. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1173. failure PCR amplification from low GC content gene - (reply: 5)
  1174. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1175. conventional v real-time PCR applications - (reply: 1)
  1176. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1177. PCR reagents, can I use reagents from different manufacturers? - (reply: 4)
  1178. different PCR primers for real-time and classic PCR - (reply: 3)
  1179. primers deconamination??? - (reply: 6)
  1180. PCR band too thick - PCR troubleshooting question (reply: 5)
  1181. RT-PCR problem - (reply: 1)
  1182. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1183. primer check!!! - (reply: 6)
  1184. PCR bands on gel electrophoresis - (reply: 5)
  1185. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1186. long range PCR - (reply: 2)
  1187. Annealing Temperature of biotinylated primers - (reply: 2)
  1188. RT-PCR - New to RT-PCR info (reply: 2)
  1189. PCR punch - (reply: 4)
  1190. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1191. PCR off plasmid for screening - (reply: 2)
  1192. PCR failed no product.. help - (reply: 10)
  1193. number of molecules in PCR - (reply: 3)
  1194. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1195. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1196. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1197. Primer dilution - PCR primer dilution (reply: 2)
  1198. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1199. Can't re-PCR the PCR product - (reply: 5)
  1200. PCR reagent autoclave - (reply: 2)
  1201. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1202. Long Primers for PCR - (reply: 2)
  1203. Data Analysis for Real-time PCR - (reply: 2)
  1204. PCR not working - overhangs too long? (reply: 3)
  1205. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1206. argh more pcr headaches! - (reply: 6)
  1207. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1208. Chlamydia PCR cycling conditions - (reply: 1)
  1209. Primers going "loopy" - (reply: 2)
  1210. Conversion of primer unit - ug/ul to uM (reply: 4)
  1211. mutagenesis by PCR or just use a kit - (reply: 5)
  1212. Unsuccessful BS PCR - (reply: 3)
  1213. Primer Trouble Shooting - (reply: 5)
  1214. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1215. Smearing on gel in PCR products - (reply: 5)
  1216. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1217. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1218. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1219. Relative RT-PCR - (reply: 2)
  1220. Relative real time RT PCR - (reply: 2)
  1221. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1222. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1223. a simple primer problem - (reply: 5)
  1224. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1225. no or poor amplification - (reply: 1)
  1226. Primers from 3'UTR - (reply: 2)
  1227. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1228. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1229. Designing primers with ESTs - (reply: 2)
  1230. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1231. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1232. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1233. Real Time vs Traditional PCR results - (reply: 1)
  1234. The problems to identify mouse genotype with PCR - (reply: 1)
  1235. addition of BclI restriction site to PCR primer - (reply: 6)
  1236. non specific amplification - DNA amplification by PCR (reply: 1)
  1237. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1238. How does methprimer calculate primer Tm - (reply: 2)
  1239. Laminar Flow vs PCR cabinet - (reply: 4)
  1240. How to do PCR to detect mRNA without RT? - (reply: 2)
  1241. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1242. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1243. PCR has stopped working - (reply: 7)
  1244. PCR negative control contamination - (reply: 8)
  1245. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1246. Phosphorylated primers - (reply: 5)
  1247. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1248. PCR efficiency in real timePCR - (reply: 5)
  1249. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1250. Drastic Decrease in PCR product yield - (reply: 2)
  1251. resources on PCR principles & technique - (reply: 1)
  1252. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1253. Colony Screeing without using PCR - (reply: 8)
  1254. High MW PCR band seen...help needed - (reply: 7)
  1255. QPCR - Primer and Probe question - QPCR (reply: 2)
  1256. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1257. standard curve/ PCR efficiency problems... - (reply: 1)
  1258. Help! PCR that used to work doesn't work now! - (reply: 2)
  1259. PCR and templates - (reply: 3)
  1260. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1261. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1262. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1263. Easy primer question? - Primers (reply: 1)
  1264. PCR inconsistency - (reply: 4)
  1265. Plasmid problem - From PCR product (reply: 7)
  1266. help needed: PCR a gene from genomic DNA - (reply: 2)
  1267. questions about pcr products after pooling - (reply: 3)
  1268. stiching/linking/sewing pcr - (reply: 3)
  1269. Human mtDNA amplification problems - (reply: 3)
  1270. primer dimer - proplems (reply: 1)
  1271. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1272. Nested PCR - (reply: 2)
  1273. pfu vs long pcr mix - (reply: 7)
  1274. weird PCR ask for help - (reply: 12)
  1275. Methylation specific DMSO PCR - (reply: 1)
  1276. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1277. No PCR product at all - (reply: 8)
  1278. PCR troubleshoooooting - primer dimer (reply: 1)
  1279. Primer efficiency test - (reply: 1)
  1280. primer software - (reply: 2)
  1281. Question on wired PCR - (reply: 1)
  1282. restriction digestion of PCR product - (reply: 7)
  1283. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1284. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1285. How to know in which exon a primer match? - (reply: 1)
  1286. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1287. Amplification of human genomic DNA - (reply: 2)
  1288. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1289. sequencing with forward/reverse primers - (reply: 7)
  1290. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1291. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1292. Adding A overhangs - primer design implications? - (reply: 1)
  1293. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1294. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1295. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1296. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1297. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1298. primer design problem - (reply: 1)
  1299. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1300. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1301. Problem with primer efficiency analysis - (reply: 4)
  1302. slan real time PCR system for validation of microarray results? - (reply: 1)
  1303. PCR unusualband at ~230bps - PCR (reply: 1)
  1304. DMSO or BSA for PCR - (reply: 6)
  1305. Whole Genome Amplification - (reply: 2)
  1306. primer design - (reply: 1)
  1307. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1308. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1309. designing primers for genes not sequenced yet - (reply: 2)
  1310. Tris Buffer for PCR reaction - why? (reply: 1)
  1311. PCR a plasmid protein - (reply: 2)
  1312. multiplex PCR - (reply: 4)
  1313. Realtime PCR machine - (reply: 2)
  1314. PCR set-up calculation nightmares. - (reply: 3)
  1315. Primer desing - (reply: 2)
  1316. Colony PCR - (reply: 3)
  1317. Standard curves for PCR efficiency. - (reply: 2)
  1318. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1319. Question about RAPD PCR - (reply: 2)
  1320. Looking for help with my PCR! - (reply: 3)
  1321. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1322. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1323. primer design - (reply: 9)
  1324. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1325. PCR without thermal cycler? - (reply: 9)
  1326. primer contamination - primer contaminated with ice (reply: 3)
  1327. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1328. RT-PCR problem - (reply: 4)
  1329. Only DNA ladder , No desired band in PCR - (reply: 4)
  1330. taq and PCR - (reply: 6)
  1331. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1332. PCR Efficiency - (reply: 2)
  1333. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1334. Long PCR and genomic DNA isolation problems - (reply: 2)
  1335. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1336. NTC with specific amplification - (reply: 1)
  1337. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1338. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1339. Primer3 vs Primer BLAST - (reply: 3)
  1340. PCR and then ligation - (reply: 7)
  1341. Primer design: free energy - (reply: 2)
  1342. Amplification in NTC and noRT controls - (reply: 5)
  1343. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1344. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1345. asymmetric PCR - (reply: 1)
  1346. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1347. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1348. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1349. trouble with pcr - (reply: 8)
  1350. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1351. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1352. Real time PCR results-interpretation - (reply: 1)
  1353. Real time PCR results-interpretation - (reply: 2)
  1354. Primer optimization for ChIP - (reply: 2)
  1355. sequencing primer: T7 or T7promoter? - (reply: 4)
  1356. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1357. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1358. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1359. primer tm - tm calculation for tagged primers (reply: 2)
  1360. Reusing 96 well plates for PCR - (reply: 2)
  1361. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1362. PCR and AFLP - (reply: 1)
  1363. Universal 16s rRNA primers needed - (reply: 3)
  1364. Identifying PCR Inhibitors - (reply: 10)
  1365. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1366. PCR + phusion enzyme = massive errors - (reply: 4)
  1367. long DNA amplification - (reply: 11)
  1368. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1369. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1370. adding koazak sequence to primer - (reply: 1)
  1371. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1372. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1373. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1374. Primer design if sequence is unknown for organism - (reply: 5)
  1375. adding C terminal tag to reverse primer - (reply: 1)
  1376. epitope tagging of pcr products - (reply: 1)
  1377. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1378. Real time PCR info - (reply: 4)
  1379. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1380. PCR from BAC - PCR from BAC (reply: 1)
  1381. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1382. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1383. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1384. How much product PCR?? - (reply: 3)
  1385. what primers should I use for DNA sequencing? please help - (reply: 2)
  1386. what primers should I use for DNA sequencing?? - (reply: 3)
  1387. Is my primer going to work for qRT-PCR? - (reply: 6)
  1388. Standard curve for RT-PCR - (reply: 1)
  1389. PCR using genomic DNA - (reply: 2)
  1390. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1391. Problem amplifying plasmid - (reply: 1)
  1392. Sybr Green I in real-time PCR reaction - (reply: 3)
  1393. Primers annealing temperatures - (reply: 8)
  1394. Difficulties cloning by PCR - (reply: 5)
  1395. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1396. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1397. high Cp value PCR - (reply: 2)
  1398. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1399. Synthesize own Oligo-dT primers - (reply: 6)
  1400. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1401. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1402. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1403. Smearing/ no amplification in PCR - (reply: 3)
  1404. primer design - Primer design for PCR (reply: 1)
  1405. How are primers designed? - (reply: 4)
  1406. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1407. DNA methylated PCR - (reply: 2)
  1408. need help on primer calcuation soon - (reply: 1)
  1409. primer design - (reply: 6)
  1410. problem in pcr and purification - (reply: 3)
  1411. Inability to duplicate PCR results - (reply: 8)
  1412. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1413. PCR using xt5 - (reply: 2)
  1414. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1415. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1416. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1417. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1418. 23S rRNA Primer - need primer sequence (reply: 1)
  1419. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1420. RT-PCR - problems with TaqMan PCR (reply: 2)
  1421. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1422. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1423. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1424. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1425. Small product amplification problems - (reply: 4)
  1426. multi way ligation or long range pcr? - (reply: 1)
  1427. Freezing primers+SYBR green - (reply: 2)
  1428. Tagged Primers - (reply: 5)
  1429. no pcr amplification product - (reply: 9)
  1430. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1431. design primer for chip - (reply: 5)
  1432. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1433. PCR problem - (reply: 4)
  1434. differently behavioring replicates in real-time PCR - (reply: 1)
  1435. Multiplex PCR - (reply: 1)
  1436. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1437. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1438. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1439. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1440. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1441. PCR components storage - (reply: 3)
  1442. reconstitution of primers - (reply: 2)
  1443. No PCR bands with some templates - (reply: 5)
  1444. Mysterious PCR Contamination - (reply: 13)
  1445. BS PCR..please help - DNA methylation analysis (reply: 15)
  1446. RT-PCR Help - Protocol provided (reply: 4)
  1447. PCR product purification - (reply: 3)
  1448. Mutagenesis PCR and undesired amplification - (reply: 2)
  1449. Temp Gradient PCR - (reply: 2)
  1450. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1451. PCR with genomic dna - (reply: 9)
  1452. primer dimer - (reply: 2)
  1453. PCR with long primers - (reply: 3)
  1454. BSP PCR standardization...help - (reply: 8)
  1455. PCR on degraded templates - (reply: 2)
  1456. PCR help needed - (reply: 3)
  1457. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1458. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1459. light bands, dark smear, dark dimers - (reply: 2)
  1460. PCR, followed by sequencing... - why ?? (reply: 3)
  1461. PCR program - strange extension step (reply: 2)
  1462. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1463. Help me! How to design nested BSP primers? - (reply: 4)
  1464. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1465. Plasmid linearization by PCR - (reply: 4)
  1466. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1467. Using BLAST to check primers - (reply: 17)
  1468. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1469. pcr doesnt show any band for DNa walking - (reply: 8)
  1470. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1471. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1472. Effects of more cDNA in PCR??? - (reply: 1)
  1473. PCR primer - (reply: 2)
  1474. PCR product disappears after restriction digestion - (reply: 4)
  1475. Primer design - GC clamp - (reply: 8)
  1476. Real Time PCR - excluding Ct - (reply: 2)
  1477. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1478. Price of a real time PCR machine? - (reply: 4)
  1479. colony PCR - (reply: 8)
  1480. probe conc. of Asymmetric PCR - (reply: 1)
  1481. primer dimer or what? - (reply: 2)
  1482. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1483. Real Time PCR method comments - (reply: 2)
  1484. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1485. colony pcr - (reply: 4)
  1486. Primers and Taqman Probes mixture question - (reply: 1)
  1487. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1488. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1489. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1490. Colony PCR - (reply: 15)
  1491. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1492. no bands in my PCR - (reply: 9)
  1493. designing primers - (reply: 1)
  1494. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1495. purefication and amplification of serum free DNA - (reply: 1)
  1496. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1497. Bisulfite Sequencing PCR - (reply: 4)
  1498. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1499. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1500. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1501. mRNA PCR - (reply: 1)
  1502. pcr product quantification - (reply: 5)
  1503. Negative Control Primers for ChIP Assay - (reply: 3)
  1504. Magnesium in PCR - (reply: 1)
  1505. PCR Sample Prep - (reply: 4)
  1506. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1507. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1508. Bizarre Contamination in PCR - (reply: 7)
  1509. PCR Cloning-large primers - (reply: 4)
  1510. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1511. forward and reverse primer - (reply: 4)
  1512. Check the primers - (reply: 4)
  1513. PCR problem from transformed TOPO TA vector - (reply: 3)
  1514. DIG DNA PCR labeling problem - (reply: 6)
  1515. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1516. BSP primers - (reply: 2)
  1517. Various size of insert after colony screen by PCR - (reply: 2)
  1518. inverse PCR molecular bilogy - (reply: 3)
  1519. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1520. failed PCR on DNA extract from blood - (reply: 6)
  1521. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1522. Primer dimer issue in real time PCR - (reply: 21)
  1523. Leaky RT-PCR - (reply: 4)
  1524. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1525. PCR, RNA, Northern Blotting???? - (reply: 1)
  1526. bizzare PCR smear - why does science hate me (reply: 7)
  1527. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1528. PCR detection of SNP - (reply: 6)
  1529. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1530. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1531. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1532. Sub-cloning sticky-end PCR products - (reply: 2)
  1533. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1534. ChIP on chip amplification problems - (reply: 11)
  1535. Genotyping PCR - (reply: 3)
  1536. PCR screening of transformed bacterial colony - (reply: 6)
  1537. Is this standard valid? - pcr product as standards (reply: 5)
  1538. primer binding - (reply: 1)
  1539. weird ChIP primers! - (reply: 1)
  1540. PCR primers - (reply: 1)
  1541. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1542. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1543. amplification in negative control in RT PCR - (reply: 2)
  1544. miRNA real time PCR by SYBR green methods - (reply: 1)
  1545. Deletion PCR - (reply: 9)
  1546. diluting to final primer concentration - please help correct (reply: 1)
  1547. Smear problem with my new primer set - (reply: 2)
  1548. PCR ghost bands - (reply: 1)
  1549. Amplifying a gene using a degenerate primer - (reply: 4)
  1550. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1551. CP of real time PCR by LC480 - (reply: 1)
  1552. CHIP- Real Time PCR calculations - (reply: 1)
  1553. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1554. transgene copy number - using real time PCR (reply: 1)
  1555. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1556. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1557. Unspecific PCR - (reply: 7)
  1558. Real-Time PCR as a Microplate Reader - (reply: 1)
  1559. gene-specific RT-PCR - (reply: 4)
  1560. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1561. RT-PCR Internal Standard - (reply: 2)
  1562. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1563. Plasmid supercoiling affecting PCR? - (reply: 2)
  1564. RNA contamination in PCR - (reply: 1)
  1565. PCR without DNA extraction! - (reply: 2)
  1566. Primer tm - (reply: 1)
  1567. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1568. re-amplification in real-time PCR - (reply: 1)
  1569. primer design - really necessary? (reply: 2)
  1570. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1571. RT-PCR - (reply: 3)
  1572. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1573. Degenerative primers = multiple products? - (reply: 5)
  1574. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1575. PCR problems on a ligation product - (reply: 5)
  1576. Genomic DNA and PCR - (reply: 4)
  1577. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1578. Sewing PCR - (reply: 3)
  1579. on the minimal pcr derived product - (reply: 2)
  1580. primer dimers - (reply: 5)
  1581. RT-PCR vs. conventional PCR - (reply: 1)
  1582. qPCR primer design - (reply: 5)
  1583. PCR and gel purification problem - did I screw this up (reply: 4)
  1584. Cloning PCR fragment - (reply: 1)
  1585. touchdown pcr - (reply: 6)
  1586. non specific pcr products - (reply: 4)
  1587. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1588. Anyone with experiences in 2 step PCR - (reply: 1)
  1589. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1590. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1591. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1592. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1593. re-amplification of a PCR product - is it recommended? (reply: 7)
  1594. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1595. PCR machine with accurate temperature control? - (reply: 1)
  1596. Gene-specific RT-PCR - (reply: 2)
  1597. Primer stock confusion - (reply: 6)
  1598. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1599. primer design - primer design question (reply: 2)
  1600. Protocol for 16S rDNA real time PCR - (reply: 2)
  1601. Touchdown PCR - (reply: 1)
  1602. Determine annealing temperature of primers - (reply: 14)
  1603. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1604. polymerase for BSP - (reply: 2)
  1605. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1606. Validating real time pcr primers - (reply: 2)
  1607. BSP,MSP primer design? - (reply: 1)
  1608. low gc primers - help with pcr using low gc primers (reply: 6)
  1609. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1610. Primers and annealing temperature - (reply: 2)
  1611. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1612. Copy number calculations in real time PCR - (reply: 2)
  1613. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1614. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1615. RT-PCR Negative controls - (reply: 1)
  1616. WHich primer to use for sequencing - (reply: 5)
  1617. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1618. minimum length for the gene to be amplified in PCR - (reply: 6)
  1619. dNTP Quantity - (reply: 3)
  1620. annoying PCR cloning problem (season 2) - (reply: 5)
  1621. PCR with 60 bp primers - results in no product (reply: 6)
  1622. Amplification dwindles using little template RT-PCR - (reply: 3)
  1623. Running gel after RT-PCR - (reply: 5)
  1624. Degenerate PCR Size Limit? - (reply: 3)
  1625. primer design for pBAD topo expression kit - (reply: 2)
  1626. PCR problems.. - problems with conventional PCR (reply: 3)
  1627. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1628. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1629. small PCR products on agarose gel? - (reply: 8)
  1630. Dnase digestion and PCR - (reply: 4)
  1631. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1632. primers fpr sequencing - (reply: 4)
  1633. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1634. Need an UV260 RT-PCR instrument - (reply: 1)
  1635. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1636. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1637. PCR produces products of wrong size - (reply: 4)
  1638. PCR with pfu and degenerate primers (?) - (reply: 7)
  1639. a doubt about PCR gel purification - (reply: 4)
  1640. Draw PCR primer locations - PCR primer (reply: 4)
  1641. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1642. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1643. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1644. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1645. PCR+enhancer - (reply: 4)
  1646. Serious issues with RT-PCR - (reply: 7)
  1647. PCR [dNTP] - dNTP concentrations (reply: 2)
  1648. PCR issue - (reply: 2)
  1649. dNTP question - (reply: 3)
  1650. PCR - (reply: 1)
  1651. long PCR primer - (reply: 7)
  1652. Taqman rtPCR primer and probe design - (reply: 3)
  1653. sequencing problem after pcr - (reply: 4)
  1654. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1655. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1656. primer design - (reply: 3)
  1657. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1658. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1659. Analysis of ChIP RT-PCR data - (reply: 7)
  1660. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1661. Negative flouroscence in real time PCR - (reply: 2)
  1662. Minimum number of PCR cycles to see a product? - (reply: 6)
  1663. decontamination - decontamination for PCR (reply: 6)
  1664. Troubleshoot ARMS PCR - (reply: 2)
  1665. negative control for PCR - (reply: 1)
  1666. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1667. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1668. pcr pdt amplification - (reply: 1)
  1669. real time PCR - (reply: 3)
  1670. PCR fails when I scale up - (reply: 2)
  1671. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1672. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1673. PCR not Working = SMEAR - (reply: 3)
  1674. How to do PCR fragment in 2 steps - (reply: 3)
  1675. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1676. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1677. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1678. RT-PCR - problem of PCR (reply: 1)
  1679. PCR product longer than template - why? - (reply: 2)
  1680. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1681. Dye recipe that can be used for PCR MM - (reply: 3)
  1682. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1683. PCR conditions with three primers - (reply: 1)
  1684. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1685. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1686. Cloning a labeled PCR product - (reply: 1)
  1687. PCR and cloning - (reply: 14)
  1688. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1689. PCR product - not suppose to be there (reply: 7)
  1690. using the meth primer - (reply: 2)
  1691. Generating primers for MSP - (reply: 1)
  1692. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1693. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1694. PCR from a smear genomic DNA ? - (reply: 5)
  1695. Primer design and need help - (reply: 11)
  1696. Setting up a multiplex PCR assay. - (reply: 2)
  1697. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1698. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1699. cDNA and RT-PCR - (reply: 8)
  1700. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1701. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1702. PCR problem - help me plsss (reply: 9)
  1703. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1704. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1705. PCR efficiencies - (reply: 4)
  1706. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1707. PCR quantification - (reply: 1)
  1708. PCR quantification - (reply: 1)
  1709. Long PCR product - (reply: 9)
  1710. problem in Pcr amplification - (reply: 7)
  1711. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1712. difference between hotstart and taq polymerase - (reply: 7)
  1713. sequencing the pcr product - (reply: 15)
  1714. Pcr amplification - primer designed from UTR regions (reply: 5)
  1715. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)