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PCR Related Discussions
  1. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  2. Plasmid Amplification Issue - (reply: 6)
  3. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  4. primer design for a gene - (reply: 2)
  5. large bands in all pcr reactions including negative control - (reply: 1)
  6. confirmation for primers dilution - (reply: 10)
  7. Problems regarding amplification of my gene - (reply: 3)
  8. PCR working, qPCR is *not*. - (reply: 6)
  9. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  10. Overlapping PCR, need help - (reply: 5)
  11. How does RT-PCR work? - (reply: 5)
  12. Denatured plasmid for pcr - (reply: 1)
  13. Possible primer dimer problem..Need solution!!! - (reply: 2)
  14. confusing with where to put primers and which ordination - (reply: 4)
  15. Help with primer concentration for sequencing - (reply: 1)
  16. PCR troubleshoot - (reply: 5)
  17. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  18. PCR Products not run well in the GEL - (reply: 1)
  19. PCR solution calculus - (reply: 4)
  20. Amplifying from pcr products - (reply: 7)
  21. Consistancy issue with PCR block - (reply: 1)
  22. Queries regarding PCR - (reply: 3)
  23. Design the primers - (reply: 10)
  24. Calculation of Taq Polymerase - (reply: 3)
  25. Best Taq for colony PCR? - (reply: 5)
  26. How two fragments joint together when doing fusion PCR? - (reply: 4)
  27. How to test my milleq water in q PCR about contaminants - (reply: 3)
  28. Mutagenesis PCR - (reply: 9)
  29. real time PCR primer design - (reply: 1)
  30. The PCR gods are frowning upon me - (reply: 9)
  31. PCR after T4 ligation? - (reply: 3)
  32. primers for cloning - (reply: 1)
  33. Degenerated Primers and their problems - (reply: 1)
  34. Sonication of small PCR amplicon - (reply: 1)
  35. primer design with restriction enzyme - (reply: 1)
  36. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  37. primer dilution help - (reply: 1)
  38. Testing primers on 'unknown' tissue - (reply: 1)
  39. How to remove inhibitory substances in PCR? - (reply: 2)
  40. need some advise about PCR of PKD1-human gene - (reply: 7)
  41. puzzled with PCR outcome after BS treatment - (reply: 2)
  42. nested PCR for low viral load- HBV patient sample - (reply: 2)
  43. troubleshooting stubborn PCR - (reply: 6)
  44. Measuring pcr fragments in a gel - (reply: 1)
  45. Amplification in HK gene but not for target gene - (reply: 3)
  46. could you help me with my stem loop RT primer? - (reply: 1)
  47. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  48. How long can I store at - 30°C my PCR mix? - (reply: 6)
  49. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  50. RT-PCR help - (reply: 2)
  51. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  52. PCR Temperature change control malfunctioning - (reply: 1)
  53. excess amount of primers - (reply: 2)
  54. Dehydrating and Reconstituting primers - (reply: 15)
  55. Trouble with PCR of short sequence - (reply: 3)
  56. Setting the temperature range for gradient PCR - (reply: 3)
  57. KIR gene promoters for MSP primers design - (reply: 2)
  58. Biotin-streptavidin signal amplification - (reply: 1)
  59. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  60. Amplification curve in the negative control samples - (reply: 7)
  61. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  62. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  63. Electrophoresis after PCR : too many bands - (reply: 6)
  64. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  65. Primer as limiting reagent in PCR reaction - (reply: 2)
  66. Primer Design for RNA probes - (reply: 2)
  67. Real time PCR for degraded RNA - (reply: 1)
  68. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  69. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  70. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  71. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  72. Nested BSP Primer Design - (reply: 6)
  73. website for primer design - (reply: 1)
  74. PCR Efficiency over 150%! - (reply: 1)
  75. problem of amplification - (reply: 2)
  76. PCR not working - (reply: 11)
  77. How to do a primer dilution - (reply: 10)
  78. Troubles with Fusion PCR - (reply: 1)
  79. Primer design and alternative transcripts - (reply: 2)
  80. PCR and sequencing of genomic DNA - (reply: 4)
  81. qPCR amplification - (reply: 4)
  82. NESTED PCR - (reply: 6)
  83. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  84. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  85. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  86. Digestion necessary after PCR? - (reply: 7)
  87. Inverse PCR product selection - (reply: 2)
  88. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  89. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  90. how to hasten real-time PCR amplifications - (reply: 2)
  91. Please please help me with my Phusion PCR. - (reply: 5)
  92. PCR - (reply: 1)
  93. NCBI Primer Design - Stringency Issues - (reply: 3)
  94. Different primer concentration in qPCR - (reply: 1)
  95. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  96. help in long pcr - (reply: 1)
  97. PCR inhibitor in template DNA - (reply: 3)
  98. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  99. Problem with Real-time PCR results analysis - (reply: 1)
  100. Primer Specificity: Testing only one primer - (reply: 4)
  101. PCR from protozoa DNA - (reply: 3)
  102. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
  103. tool for comparing many primers pairs - (reply: 4)
  104. PCR that leads to protein synthesis - (reply: 18)
  105. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  106. Use of DMSO in General PCR - (reply: 1)
  107. PCR product size confusion - (reply: 3)
  108. Pcr primers - (reply: 7)
  109. Concentration specification in PCR - (reply: 3)
  110. Guanidine isothiocyanate in PCR - (reply: 1)
  111. Primers have worked well but now getting primer dimers? - (reply: 2)
  112. I cannot design primers on exon-exon junction - (reply: 2)
  113. DNA Quantification of PCR Products - (reply: 2)
  114. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  115. Problem for PCR - (reply: 9)
  116. Designing primers in UTRs - (reply: 1)
  117. Multiplex PCR - (reply: 1)
  118. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  119. primer design@ buy? - (reply: 2)
  120. Trouble with overlap extension pcr - (reply: 3)
  121. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  122. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  123. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  124. Question about the RT PCR - (reply: 3)
  125. PCR ready mixes with long shelf lives - (reply: 4)
  126. Troubleshooting: Inverse PCR - (reply: 3)
  127. How to design primer to amplify genomic DNA? - (reply: 3)
  128. Overlap PCR, need help - (reply: 11)
  129. Internal control for miRNA RT-PCR - (reply: 1)
  130. Primers - (reply: 1)
  131. Primers mix - (reply: 2)
  132. question about RNA concentration for real time PCR - (reply: 1)
  133. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  134. PCR products sizes and DNA ladder - (reply: 7)
  135. Having problem with primers for qPCR - (reply: 4)
  136. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  137. PCR product sequencing - (reply: 3)
  138. Bad fragment amplification - (reply: 4)
  139. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  140. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  141. No amplification with TRAPEZE kit! - (reply: 1)
  142. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  143. Stargazer PCR problems - (reply: 3)
  144. General PCR discussion - (reply: 8)
  145. Real time. No amplification but hight flourescence - (reply: 1)
  146. Real time PCR doubt - (reply: 5)
  147. PCR Profile for ligation - (reply: 3)
  148. protocol to relieve melanin inhibition of PCR - (reply: 4)
  149. No bands despite different primers and conditions and TAqs - (reply: 8)
  150. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  151. Equimolar Mix Primer - (reply: 8)
  152. Wrong PCR product - (reply: 2)
  153. Colony PCR positive and Digestion negative????? - (reply: 11)
  154. Primer design - (reply: 5)
  155. storage for lyophilized primers - (reply: 1)
  156. PCR GAPDH gene - (reply: 1)
  157. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  158. Quantitative RT-PCR statistics help - (reply: 1)
  159. Dimerization of PCR product - (reply: 4)
  160. PCR Primer trouble - (reply: 2)
  161. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  162. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  163. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  164. finding the corrosponding primers - (reply: 2)
  165. Gene sequence for Real time PCR - (reply: 2)
  166. non-reproducible PCR results with cDNA as template - (reply: 1)
  167. Ligation of PCR fragments - (reply: 11)
  168. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  169. RNA extraction for RT-PCR - (reply: 3)
  170. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  171. problems with gDNA doing real time PCR in yeast - (reply: 2)
  172. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  173. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  174. How to get rid of bands in PCR negative control - (reply: 10)
  175. T7 and M13 primers two band amplification - (reply: 3)
  176. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  177. Strange PCR problem - (reply: 2)
  178. Question about Double Digestion followed by PCR amplification - (reply: 2)
  179. PCR problem - (reply: 2)
  180. PCR machine not working properly - (reply: 1)
  181. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  182. PCR gene specific amplification problem - (reply: 3)
  183. Failure SYBRGREEN PCR - (reply: 4)
  184. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  185. without RE site in PCR product - (reply: 5)
  186. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  187. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  188. real-time pcr non reproducible - (reply: 4)
  189. Strange amplification plots with high Ct variability - (reply: 1)
  190. How big a role does mixing play in PCR - (reply: 1)
  191. Melting curve is irregular for primer optimization - (reply: 5)
  192. Designing primers for ABO blood groups - (reply: 1)
  193. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  194. RT-PCR primer design - (reply: 7)
  195. How to amplify very short PCR template - (reply: 4)
  196. Is this primer okay? - (reply: 4)
  197. PCR amplification of large template - (reply: 1)
  198. PCR insert - in frame? - (reply: 2)
  199. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  200. smear in gel electrophoresis after PCR - (reply: 2)
  201. Whole plasmid amplification by PCR - (reply: 2)
  202. PCR product as standard curve template - (reply: 6)
  203. colony PCR after transformation - (reply: 1)
  204. PCR machine - (reply: 3)
  205. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  206. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  207. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  208. Resuspending primers calculation - (reply: 4)
  209. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  210. PCR RFLP is PCR product purification necessary? - (reply: 3)
  211. Mystery in my PCR - (reply: 5)
  212. Whole genome amplification from cDNA? - (reply: 4)
  213. What do you use to check primer secondary structure? - (reply: 3)
  214. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  215. PCR product as template for in vitro transcription - (reply: 1)
  216. Problem with 3 step PCR - (reply: 3)
  217. mutagenic primers with very high GC content. - (reply: 3)
  218. Scaling up PCR to get more DNA - (reply: 5)
  219. PCR to get 10kbp product - (reply: 4)
  220. site-directed mutagenesis primer Tm - (reply: 4)
  221. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  222. Random vs oligo primer in preamplification RT - (reply: 1)
  223. how does polymerase stop at the required length - (reply: 1)
  224. Chip pcr. are there inespecifics? - (reply: 1)
  225. Primer concentration - stupid question - (reply: 3)
  226. Primer Design, help i´m New - (reply: 4)
  227. No DNA after PCR product purification - (reply: 9)
  228. Sensitivity of RT-PCR and qPCR - (reply: 4)
  229. RT-qPCR primer problem - (reply: 4)
  230. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  231. real time PCR trouble - (reply: 3)
  232. removal of ethanol in PCR product purification - (reply: 5)
  233. Crazy real time PCR curve - (reply: 4)
  234. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  235. High fidelity PCR trouble shooting - (reply: 2)
  236. Designing cloning primers for DNMT - (reply: 2)
  237. Reproducible Non-Specific PCR Product - (reply: 2)
  238. Are these primer products good enough for qPCR? - (reply: 3)
  239. Can't get PCR with large overhang primers to work - (reply: 8)
  240. Primer Efficiency across runs - (reply: 1)
  241. Question about pipet tips for PCR and rtPCR - (reply: 4)
  242. UNG in PCR - (reply: 1)
  243. Cause of random samples failing PCR? - (reply: 2)
  244. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  245. pcr 10X buffer preparation - (reply: 2)
  246. how much template do i need for pcr? - (reply: 5)
  247. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  248. Problems with crossover PCR - (reply: 2)
  249. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  250. Designing PCR Primers for cloning - (reply: 17)
  251. Creating primers to add restriction sites to vector backbone - (reply: 7)
  252. PCR DNA Concentration - (reply: 1)
  253. RT-PCR product- no band - (reply: 4)
  254. Some primers dose not work - (reply: 5)
  255. Understanding RACE PCR - (reply: 1)
  256. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  257. Wierd Bands after PCR....Confused - (reply: 9)
  258. Query regarding primers for quick change mutagenesis - (reply: 3)
  259. PCR with Plasmid recovered from filter paper - (reply: 6)
  260. PCR amplified product size - (reply: 5)
  261. the storage time for primers - (reply: 9)
  262. Annealing temperature for PCR - (reply: 8)
  263. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  264. Nested PCR - (reply: 2)
  265. random primers or oligodT - (reply: 4)
  266. No amplification during RT-PCR - (reply: 4)
  267. Sequencing RT-PCR product - (reply: 3)
  268. Low yield PCR product after gel purification - (reply: 8)
  269. To design or use published primers? - (reply: 4)
  270. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  271. PCR primer usage (Clonning & cDNA) - (reply: 2)
  272. problem in cloning PCR primer design with restriction site - (reply: 4)
  273. Amplification with U primers, but not with M primers - (reply: 1)
  274. Taq polymerase - (reply: 7)
  275. His tag introduction in to gene and primer design - (reply: 2)
  276. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  277. Which High-Fidelity polymerase is better? - (reply: 2)
  278. polymerase to use for cloning - (reply: 4)
  279. Problem amplifying viral gene - (reply: 5)
  280. Adding tag using overlapping PCR - (reply: 2)
  281. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  282. Design primer from incomplete sequence... - (reply: 2)
  283. Design primer from incomplete sequence... - (reply: 2)
  284. How to perform colony PCR - (reply: 1)
  285. Primer designing for Methylation - (reply: 6)
  286. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  287. cDNA amplification problem - (reply: 4)
  288. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  289. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  290. PCR product running on agarose gel - (reply: 32)
  291. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  292. No bands in PCR after DNase treatment - (reply: 4)
  293. PCR amplification with high fidelity enzyme - (reply: 1)
  294. PCR HELP!!!! - (reply: 1)
  295. Overlap PCR problem - (reply: 5)
  296. PCR and restriction enzyme digestion - (reply: 3)
  297. Bisulfite PCR and cloning - (reply: 5)
  298. PCR product purification - (reply: 4)
  299. Overlapping sequence PCR primers - (reply: 1)
  300. restricted PCR plasmid runs slower - (reply: 2)
  301. BSP PCR primer design explained - (reply: 11)
  302. Bisulfite sequencing PCR worked - (reply: 5)
  303. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  304. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  305. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  306. doing PCR using PCR product , help plz !! - (reply: 5)
  307. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  308. good 16S univ. primer for qPCR? - (reply: 3)
  309. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  310. Amplification of CAG promoter problems - (reply: 4)
  311. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  312. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  313. I need help for designing a pair of primers for this sequence. - (reply: 1)
  314. PCR not working for 5 Aza treated DNA - (reply: 3)
  315. Differentiating mouse from human cells with PCR - (reply: 5)
  316. Differentiate between RT-PCR and PCR - (reply: 3)
  317. PCR band slightly BELOW expected length ?! - (reply: 14)
  318. long primers PCR - (reply: 4)
  319. unexpected band in PCR with plasmid - (reply: 4)
  320. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  321. DNA polymerase for GC rich template? - (reply: 4)
  322. Sequencing Primers and Plasmids - (reply: 3)
  323. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  324. DNA extraction - PCR Problem - (reply: 3)
  325. Primers to amplify Kan operon from pet28 - (reply: 2)
  326. PCR product biotinylation - (reply: 3)
  327. Tagged primers and a second PCR targetting the tags - (reply: 5)
  328. cloning pcr insert into plasmid - (reply: 2)
  329. Barcode generator for PCR primers - (reply: 2)
  330. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  331. Barcoding PCR products - (reply: 18)
  332. Biotinylated primers - (reply: 4)
  333. role of water in PCR - (reply: 3)
  334. HIV gene- PCR - (reply: 3)
  335. Real time PCR melting curve - (reply: 2)
  336. BSA in PCR reaction - (reply: 1)
  337. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  338. Cannot see insert with colony pcr - (reply: 6)
  339. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  340. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  341. How to Join two PCR products - (reply: 3)
  342. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  343. What's wrong with my PCR - (reply: 5)
  344. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  345. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  346. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  347. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  348. Cp, but no amplification - (reply: 2)
  349. pcr product digestion problem - (reply: 4)
  350. Low PCR product - (reply: 7)
  351. Control primers for bisulfite-converted DNA - (reply: 3)
  352. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  353. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  354. wrong dntp concentration - (reply: 2)
  355. Third round PCR - (reply: 2)
  356. long fragment PCR - (reply: 2)
  357. Best proof-reading polymerase? - (reply: 12)
  358. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  359. Q regarding primer mix for MSP!! - (reply: 4)
  360. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  361. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  362. I need to break this cycle of PCR issues - (reply: 13)
  363. Questions / Help with Fusion PCR - (reply: 8)
  364. Controls for RT-PCR reactions - (reply: 2)
  365. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  366. BSP Primers with M13 Tails - (reply: 1)
  367. Detection of T7 RNA polymerase by WB - (reply: 6)
  368. Multiplex PCR - (reply: 33)
  369. issues when designing PCR primers using PRIMER3 - (reply: 8)
  370. Primer catalog/databasing - (reply: 1)
  371. Nonrepetitive nested PCR - (reply: 2)
  372. Primer Blast - (reply: 6)
  373. Help designing primers for use in In-fushion cloning - (reply: 4)
  374. PCR internal control - (reply: 2)
  375. Primers Freeze-Thaw - (reply: 16)
  376. TDNA Genotyping PCR program - (reply: 1)
  377. primer design using 16s rRNA - (reply: 1)
  378. question about PCR and cloning - (reply: 1)
  379. PCR - (reply: 2)
  380. nonspecific band at 50bp in PCR - (reply: 9)
  381. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  382. PCR master mix - (reply: 7)
  383. Maximum PCR product Tm - (reply: 6)
  384. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  385. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  386. PCR & Southern blotting - (reply: 1)
  387. Bisulfite PCR Question - (reply: 2)
  388. Primer Design HELP! - (reply: 3)
  389. Help with sequencing of a 120bp PCR product. - (reply: 9)
  390. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  391. How are Primers made? - (reply: 9)
  392. Long Non-Specific PCR Products - (reply: 5)
  393. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  394. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  395. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  396. PCR calculation - (reply: 1)
  397. Colony PCR and digestion with KpnI - (reply: 10)
  398. designing methylation specific primers - (reply: 3)
  399. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  400. PCR - consistent false positive results - (reply: 6)
  401. PCR no amplification - (reply: 15)
  402. How to quantify virus by PCR - (reply: 12)
  403. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  404. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  405. Pcr product size determination for a fusion gene - (reply: 8)
  406. Quantify DNA before ChIP PCR - (reply: 3)
  407. Primer Annealing Temperatures - (reply: 24)
  408. my pcr product is larger than expected - (reply: 4)
  409. Question about PCR - DNA or RNA as template - (reply: 4)
  410. pcr purification - (reply: 1)
  411. pcr purification doubt - (reply: 4)
  412. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  413. PCR band moves lower than its size on agarose gel - (reply: 3)
  414. same primers, different product in conventional PCR and qPCR - (reply: 6)
  415. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  416. pcr problem - (reply: 6)
  417. NEB Q5 polymerase works very well - (reply: 1)
  418. Primers amplify genomic DNA but not cDNA - (reply: 2)
  419. pcr purification - (reply: 1)
  420. storing PCR product overnight - (reply: 2)
  421. Primer BLAST - (reply: 1)
  422. Bands in negative control PCR - (reply: 2)
  423. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  424. problem with pcr mutagenesis - (reply: 10)
  425. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  426. Use PCR purification kit to purify restriction digestion - (reply: 2)
  427. PCR primer design - published primers trustable? - (reply: 8)
  428. Polymerase for E. coli screenings - (reply: 5)
  429. complicated RT-PCR issues - (reply: 4)
  430. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  431. Can we use degenerate bases in BSP primers? - (reply: 1)
  432. Quick change mutagenesis No PCR product visible - (reply: 2)
  433. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  434. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  435. Can anyone help with my Bisulfite PCR? - (reply: 2)
  436. How to check primer sequences using BLAST and other tools? - (reply: 2)
  437. real time PCR analysis in patient samples - (reply: 2)
  438. carpet in gels for PCR products - (reply: 2)
  439. Primer Check? - (reply: 2)
  440. Sequencing of the PCR production in BSP - (reply: 1)
  441. amplification of 14Kb - (reply: 1)
  442. Validation of PCR primers/ probes - (reply: 6)
  443. Taq polymerase for MSP - (reply: 1)
  444. Real time PCR sudenly not working - (reply: 1)
  445. Buffer-composition One-Step RT-PCR - (reply: 4)
  446. Two reverse primer sequences for a single forward primer - (reply: 1)
  447. PCR with more than one primer - (reply: 1)
  448. RAPD - PCR problem - (reply: 5)
  449. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  450. How to best store PCR product? - (reply: 2)
  451. RT-PCR primer design - (reply: 1)
  452. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  453. Odd gel run following PCR - (reply: 28)
  454. Qiagen PCR Array Reagents? - (reply: 1)
  455. No or very weak band using 16s primers - (reply: 1)
  456. No PCR amplification with b-actin primers - (reply: 1)
  457. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  458. Site-directed, PCR works but no colonies - (reply: 2)
  459. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  460. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  461. No PCR amplified with long primers - (reply: 10)
  462. PCR optimization: PCR vs qPCR - (reply: 4)
  463. should I do blunt end my pcr product before ligation - (reply: 2)
  464. PCR smear for genomic samples - (reply: 4)
  465. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  466. another primer dilution question - (reply: 2)
  467. i need help with virus pcr - (reply: 2)
  468. Primer Efficiency - (reply: 2)
  469. Experimental set up for qRT PCR - (reply: 13)
  470. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  471. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  472. How should I optimize my PCR - (reply: 2)
  473. Second round of PCR after gel extraction fails miserably - (reply: 3)
  474. an effective way to do a yeast colony pcr - (reply: 2)
  475. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  476. urgent help plz with RT-PCR - (reply: 3)
  477. oligo(dt) 15 vs random primers - (reply: 3)
  478. Help to optain a long PCR produc - (reply: 3)
  479. Multiple bands in eluted PCR product - (reply: 1)
  480. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  481. Problems with two step RT-PCR - (reply: 2)
  482. Getting genomic DNA for PCR - (reply: 4)
  483. primer dilutions - (reply: 4)
  484. Genotyping PCR primers - (reply: 1)
  485. Conventional PCR - (reply: 1)
  486. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  487. PCR product loss on cleanup - (reply: 2)
  488. MSP Primers not working - (reply: 6)
  489. Primers too dilute - (reply: 1)
  490. Amplification curves jagged - (reply: 1)
  491. Cloning HELP- Possible Primer issues - (reply: 6)
  492. No PCR product in site-directed mutagenesis - (reply: 4)
  493. Giardia/Crypto real time PCR late amplification - (reply: 1)
  494. How to dilute the template DNA for PCR - (reply: 7)
  495. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  496. Need help for PCR - (reply: 5)
  497. PCR primer pairs - university exam - (reply: 7)
  498. unespecific band PCR from mouse genomic DNA - (reply: 9)
  499. Help with primer design through PRIMER3 - (reply: 4)
  500. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  501. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  502. Plasmid vs cDNA amplification by PCR - (reply: 2)
  503. Tm of primers - prediction and verification - (reply: 1)
  504. PCR cloning...help! - (reply: 5)
  505. RT-PCR contamination in negative control. - (reply: 1)
  506. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  507. how to design primers for 16sr RNA - (reply: 24)
  508. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  509. Help with primer design - (reply: 3)
  510. Primer Tm calculation. - (reply: 1)
  511. low concentration of purified PCR product - (reply: 2)
  512. PCR smearing and...problems! - (reply: 7)
  513. Running Primers on Agarose Gel - (reply: 6)
  514. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  515. universal primers for PCR ribotyping - (reply: 6)
  516. will the template of PCR be added A ? - (reply: 2)
  517. Colony PCR not working - (reply: 4)
  518. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  519. Primer design: Tm above or below Ta? - (reply: 5)
  520. Oligo primers for shRNA construction - (reply: 1)
  521. Design primers for expression cloning - (reply: 11)
  522. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  523. primer dilution query - (reply: 2)
  524. Real Time PCR polymerase enzyme - (reply: 3)
  525. Estimating size of PCR products in a agarose gel - (reply: 4)
  526. Sequencing of PCR product - (reply: 4)
  527. PCR contamination problem - (reply: 2)
  528. Using BLAST to check primers - (reply: 1)
  529. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  530. What is the function of a third primer in a PCR mix?? - (reply: 2)
  531. How can we design a primer ? step by step method - (reply: 4)
  532. cDNA synthesis or RT-PCR - (reply: 3)
  533. PCR on cDNA and SGamplification??? - (reply: 1)
  534. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  535. real time PCR protocol - controls? - (reply: 2)
  536. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  537. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  538. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  539. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  540. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  541. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  542. PCR and UV spectrophotometry - (reply: 3)
  543. RT-PCR in a single tube - (reply: 3)
  544. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  545. annealing temperature for 3 primer pairs? - (reply: 4)
  546. PCR Reaction - (reply: 3)
  547. Impact of primer sequence on TA cloning - (reply: 3)
  548. problems with Bisulfite PCR - (reply: 1)
  549. low pcr product - (reply: 3)
  550. overlapping PCR protocol - (reply: 12)
  551. PCR to amplify my insert not working - (reply: 6)
  552. Problem with PCR amplification- high 3' stability - (reply: 3)
  553. Home made real-time PCR problem - (reply: 4)
  554. High RNase concentration interfering with PCR? - (reply: 8)
  555. Pcr primers - (reply: 2)
  556. PCR failure from yeast genomic DNA - (reply: 1)
  557. failure of PCR from yeast genomic DNA - (reply: 1)
  558. RT-Q PCR primer design question - (reply: 2)
  559. high MW dimers - (reply: 2)
  560. Strange RT-PCR amplification plots - (reply: 9)
  561. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  562. What products will I get from this pcr? - (reply: 1)
  563. PCR buffer without Tris - (reply: 1)
  564. PCR Water - (reply: 4)
  565. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  566. Which Brand of real-time PCR machine - (reply: 6)
  567. Problem with PCR - large extra bands - (reply: 3)
  568. big differnece in my primers Tm - (reply: 2)
  569. Designing primers for the ORFs - (reply: 9)
  570. Two-Step PCR - (reply: 2)
  571. Gradient PCR - (reply: 2)
  572. PCR with phophorylated primers - (reply: 1)
  573. Problem with New qPCR primers - help! - (reply: 6)
  574. Designing primers for miRNA located on minus strand. - (reply: 2)
  575. Plasmid sequencing without primers - (reply: 1)
  576. Smallest insert in PCR - (reply: 6)
  577. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  578. loop-mediated amplification - (reply: 2)
  579. Blunt end PCR product ligation - (reply: 1)
  580. Help with high Ct values for Real-Time PCR - (reply: 1)
  581. Genotyping by allele specific PCR - (reply: 6)
  582. RT-PCR primers and the poly(A) tail - (reply: 2)
  583. NO Insert found in the Colony PCR - (reply: 15)
  584. No band in PCR - (reply: 5)
  585. overlapping primer - (reply: 1)
  586. PCR primers help - (reply: 5)
  587. PCR smear after one-month storage - (reply: 2)
  588. Primer dimers - (reply: 2)
  589. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  590. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  591. Simple question of dNTP's for PCR - (reply: 5)
  592. Need help for PCR for AT rich gene - (reply: 3)
  593. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  594. Primer validation and PCR efficiency - (reply: 4)
  595. How to calculate the price of PCR test per sample? - (reply: 6)
  596. Contamination in negative control of PCR (No template control) - (reply: 6)
  597. cloning with nested pcr - (reply: 3)
  598. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  599. PCR is amplifying non-specific fragment - (reply: 6)
  600. ChIP amplification problems - (reply: 1)
  601. real time RT-PCR problems - (reply: 2)
  602. should I design primers spanning both intron and exon - (reply: 2)
  603. RT-PCR - (reply: 1)
  604. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  605. Template DNA (plasmid) concentration for PCR - (reply: 4)
  606. PCR with a very long and a short primer - (reply: 5)
  607. Ligation of 16kb PCR product - (reply: 5)
  608. Multiplex PCR does not work - (reply: 3)
  609. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  610. Problem with genomic PCR (2.4 kb) - (reply: 1)
  611. Comparison between two primers pairs? Is it possible? - (reply: 3)
  612. Rt-PCR primer design - (reply: 7)
  613. Length of non-binding sequence in primers with RE site - (reply: 6)
  614. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  615. Getting a smaller PCR product than required - (reply: 6)
  616. Difficult sequences in PCR - (reply: 2)
  617. why is real time pcr curve like this? - (reply: 3)
  618. PCR inconsistency - (reply: 2)
  619. Orientation of primers - (reply: 2)
  620. PCR, neg control & no. of cycles - (reply: 2)
  621. Strange PCR result from genomic DNA - (reply: 3)
  622. Bright bands of PCR products stick in gel wells - (reply: 1)
  623. methylation analysis with MS-HRM (primer design) - (reply: 1)
  624. whole plasmid pcr? - (reply: 6)
  625. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  626. salts in PCR - (reply: 4)
  627. Strange amplification plot - (reply: 4)
  628. Conventional PCR of different dilutions - (reply: 1)
  629. primer resuspension - (reply: 2)
  630. Principles of overlap PCR - (reply: 7)
  631. ligation of pcr product into expression vector - (reply: 4)
  632. Unable to PCR!!!! - (reply: 3)
  633. Help designing primers with restriction sites - (reply: 3)
  634. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  635. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  636. Abnormal amplification curve - (reply: 1)
  637. Problem with Semiquantitative PCR - (reply: 4)
  638. Advice on optimising bisulfite PCR sought - (reply: 2)
  639. Help: I am loosing the DNA during PCR purification - (reply: 7)
  640. PCR primer with no extra DNA end - (reply: 5)
  641. PCR colonies of Pichia pastoris - (reply: 1)
  642. ROX reference dye for quantitative RT-PCR - (reply: 1)
  643. PCR problem - (reply: 9)
  644. why PCR product smeared? - (reply: 2)
  645. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  646. silincing box, no amplification - (reply: 3)
  647. Comparative semiquantitative RT-PCR - (reply: 2)
  648. Bands in my PCR Controls - (reply: 3)
  649. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  650. Strange band in colony PCR - (reply: 2)
  651. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  652. Multiplex PCR - (reply: 12)
  653. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  654. PYO pcr stopped working. - (reply: 1)
  655. Bands seen with qPCR missing in regular PCR - (reply: 5)
  656. checking msp primers - (reply: 2)
  657. PCR product one day, none the next day - (reply: 4)
  658. PCR on colony - (reply: 10)
  659. PCR reaction ISSUES???? need help - (reply: 4)
  660. PCR using CDNA as template - (reply: 2)
  661. RT-PCR doesn't work with all RNA used - (reply: 1)
  662. Need help amplifying repeating sequence. - (reply: 5)
  663. Real Time PCR 101...help! - (reply: 1)
  664. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  665. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  666. Another primer dimers problem - (reply: 23)
  667. Colony PCR Problem!!!! - (reply: 5)
  668. using PCR product as standard template - (reply: 3)
  669. PCR kit - (reply: 7)
  670. Make additional cycles with an already finished pcr product - (reply: 3)
  671. Amplification in water control but not in samples - (reply: 3)
  672. PCR products form with DNA, but not with c-DNA - (reply: 2)
  673. question about pcr amplification efficiency? - (reply: 1)
  674. DNA polymerase: recombinant or native? - (reply: 3)
  675. pcr clean-up fail? - (reply: 1)
  676. MSP U primers very difficult to amplify... - (reply: 4)
  677. No gene amplification, 18s amplification is fine - (reply: 1)
  678. ChIP-qPCR gives odd amplification plot - (reply: 5)
  679. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  680. sequential cloning of multiple PCR products - how to do? (reply: 3)
  681. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  682. Primer design - (reply: 3)
  683. Tomato Actin PCR+Images included - (reply: 2)
  684. single primer PCR - (reply: 5)
  685. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  686. cDNA synthesis + amplification - (reply: 2)
  687. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  688. LacZ PCR Problems - (reply: 1)
  689. cells stably expressing T7 RNA polymerase - (reply: 1)
  690. Primer concentration - (reply: 4)
  691. nested PCR with low target DNA? - (reply: 2)
  692. RT-PCR calibrator - (reply: 2)
  693. RNA - extraction and pcr (reply: 1)
  694. problem in pcr - (reply: 2)
  695. Can someone recommend me a virtual PCR software? - (reply: 6)
  696. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  697. Primer 3' mismatch - Strange experience (reply: 9)
  698. real-time PCR after restriction enzyme digestion - (reply: 6)
  699. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  700. Adding loading/tracking dye to PCR mix? - (reply: 10)
  701. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  702. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  703. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  704. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  705. how to get rid of non specific bands in PCR - (reply: 1)
  706. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  707. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  708. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  709. PCR cloning problem - could not get colony (reply: 4)
  710. pcr contamination - (reply: 4)
  711. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  712. Large Non-specfic bands in PCR - (reply: 3)
  713. PCR clonning - (reply: 3)
  714. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  715. 2 bands in blue colony PCR - (reply: 5)
  716. Primer dilution --> problem.. - (reply: 16)
  717. Help! Smear in Real-Time PCR Product - (reply: 1)
  718. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  719. Sybr green RT-qPCR primer - (reply: 1)
  720. Sybr green RT-qPCR primer - (reply: 1)
  721. primer design - microalgae (reply: 2)
  722. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  723. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  724. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  725. PCR yield in ng/ul - (reply: 1)
  726. No Amplification in PCR - (reply: 4)
  727. Real time PCR and percentage loss - (reply: 1)
  728. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  729. Problem with smear in PCR with certain templates - (reply: 4)
  730. Real time PCR in the presence of heme - (reply: 3)
  731. issues in PCR amplification - (reply: 2)
  732. Problems with PCR in general - (reply: 1)
  733. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  734. how to overcome primer contamination - need protocol (reply: 11)
  735. RT-PCR primer design - Primer design and evaluation (reply: 7)
  736. CHIP analysis by PCR - (reply: 4)
  737. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  738. Primer,probes dilution - (reply: 3)
  739. Splice varient quantification in RT real time PCR? - (reply: 1)
  740. negative control for msp pcr - (reply: 2)
  741. Calculating geometric mean for real-time PCR - (reply: 5)
  742. template transfer? - how does the rna polymerase switches template? (reply: 1)
  743. PCR Gel nonspecific band - (reply: 13)
  744. semiquantitative pcr - (reply: 1)
  745. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  746. PCR: Cloning Primers - (reply: 8)
  747. PCR product - (reply: 5)
  748. pcr amplify an insert in plasmid? - (reply: 2)
  749. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  750. Exon Spanning PCR Primers - (reply: 1)
  751. Colony PCR - (reply: 1)
  752. PCR to confirm double crossover - (reply: 1)
  753. Primer sequences - (reply: 1)
  754. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  755. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  756. PCR Primers - (reply: 2)
  757. Cleaning up RT-PCR product before sequencing - (reply: 1)
  758. Template Amount for PCR Amplification - (reply: 6)
  759. How to blast methylation specific and unspecific primers - (reply: 1)
  760. RT-PCR cDNA synthesis - (reply: 6)
  761. problems amplifying from cDNA - (reply: 4)
  762. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  763. No DNA after PCR purification with QiaQuick? - (reply: 4)
  764. restriction digestion against colony PCR - (reply: 1)
  765. How to check primers are of correct sequence or not? - (reply: 2)
  766. Primers... - (reply: 1)
  767. When are primer dimers a problem? - (reply: 1)
  768. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  769. PCR: always got band in the negative control - (reply: 8)
  770. primer with higher melting temperature - (reply: 2)
  771. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  772. Problems designing primers for BSP - (reply: 2)
  773. qPCR primer design - possible off-target (reply: 1)
  774. Primer dimer formation and real-time PCR - (reply: 7)
  775. Identify PCR products - (reply: 3)
  776. PCR genomic DNA using cell lysate - (reply: 1)
  777. Maximum size of overhangs in PCR - (reply: 6)
  778. Colony PCR doesn't work anymore - (reply: 5)
  779. RT-PCR t-test, and ANOVA - (reply: 1)
  780. Primer Design Urgent! - (reply: 1)
  781. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  782. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  783. PCR of microRNA first strand cDNA - (reply: 1)
  784. how to know my primer's sense ??? - help ! (reply: 2)
  785. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  786. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  787. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  788. intron spanning primers for non model organisms - (reply: 2)
  789. Primer design - (reply: 2)
  790. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  791. qPCR amplification interference fixed with freezing - (reply: 6)
  792. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  793. software for quantifing PCR product band - (reply: 1)
  794. Multiple bands from purified PCR product - (reply: 3)
  795. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  796. Doing PCR on Nebulized DNA - (reply: 2)
  797. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  798. RT-PCR - (reply: 9)
  799. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  800. inverse pcr - (reply: 2)
  801. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  802. Primer seq - (reply: 2)
  803. Urgent: should you dry PCR product - (reply: 13)
  804. PCR product size - (reply: 6)
  805. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  806. oligo dt and random primer - (reply: 3)
  807. mRNA Search for RT-PCR (U to T) - (reply: 2)
  808. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  809. PCR Mastermix - (reply: 4)
  810. PCR triplicates versus one reaction - differences? (reply: 17)
  811. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  812. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  813. Primers and self and hetero dimers - (reply: 1)
  814. Really weird amplification curves - (reply: 1)
  815. Primers for qPCR - (reply: 3)
  816. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  817. RT-PCR primer - (reply: 3)
  818. PCR problem - basic PCR for plasmid amplification (reply: 2)
  819. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  820. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  821. Unpredictablity of PCR product - (reply: 5)
  822. total cDNA amplification by PCR - (reply: 2)
  823. Detection limit of a conventional PCR - (reply: 2)
  824. PCR for MULTIPLE mutagenesis - (reply: 4)
  825. Using Sybr green in realtime pcr - (reply: 1)
  826. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  827. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  828. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  829. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  830. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  831. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  832. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  833. Digested PCR product migrate slower than uncut - (reply: 8)
  834. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  835. PCR question - (reply: 2)
  836. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  837. 2 rounds PCR got problem - (reply: 2)
  838. PCR help minus strand - Primer design (reply: 1)
  839. chloramphenicol storage and amplification - (reply: 3)
  840. Smear in long distance PCR - (reply: 39)
  841. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  842. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  843. TOUCH DOWN PCR - (reply: 2)
  844. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  845. PCR stopped working - After changing buffer (reply: 5)
  846. ChIP PCR question - (reply: 33)
  847. PCR protocol questions! - (reply: 2)
  848. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  849. DNA amount calculation for PCR - (reply: 14)
  850. ChIP primer design - (reply: 1)
  851. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  852. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  853. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  854. How to analyze ChIP PCR data - (reply: 10)
  855. Questions regarding RT-PCR optimization - (reply: 2)
  856. number of copies after pcr? - (reply: 1)
  857. PCR double-bands - (reply: 1)
  858. Problems with semi-quatitative PCR - (reply: 1)
  859. Question about Primers - (reply: 3)
  860. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  861. Large fragment amplification failed - (reply: 3)
  862. purification of PCR product for cloning in a vector - (reply: 3)
  863. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  864. PCR reaction calculation - (reply: 2)
  865. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  866. Primer design with a tag - (reply: 4)
  867. no increase in fluorescence in my real time PCR - (reply: 3)
  868. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  869. Help with real-time PCR quantification of miRNA - (reply: 8)
  870. PCR, using gel extracted band as a template - (reply: 4)
  871. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  872. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  873. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  874. Combining forward and reverse primer gives different size on gel - (reply: 2)
  875. Q-PCR and RT-PCR - (reply: 3)
  876. calculating total amount - in a pcr reaction (reply: 4)
  877. Problems with amplifying microRNA - (reply: 3)
  878. PCR of complement genomic DNA - (reply: 3)
  879. primers for fungal identification - (reply: 9)
  880. confusing PCR - (reply: 1)
  881. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  882. PCR for long and repetitive region from genomic DNA - (reply: 4)
  883. difference between PCR primers and sequencing primers - (reply: 1)
  884. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  885. PCR of repeated region - (reply: 1)
  886. Sense and antisense DNA, and primer design - (reply: 6)
  887. PCR bands in NTC but NOT in negative samples - (reply: 2)
  888. housekeeping genes in PCR - (reply: 1)
  889. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  890. Question about RealTime PCR Primer Design - (reply: 1)
  891. Designing a Primer with a RS that has a W - (reply: 1)
  892. Primers stopped working!? - PCR Primers (reply: 6)
  893. NFK beta primers - (reply: 4)
  894. final PCR product - need clarifications (reply: 1)
  895. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  896. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  897. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  898. RT-PCR result !! - (reply: 4)
  899. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  900. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  901. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  902. why genomic can't be use for pcr? - (reply: 2)
  903. Primer design - Primer design (reply: 2)
  904. Re-use unamplified PCR product - (reply: 2)
  905. Reason for odd PCR conditions - (reply: 3)
  906. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  907. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  908. pcr - problem in pcr (reply: 2)
  909. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  910. How to present and analyze these real-time PCR data? - (reply: 2)
  911. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  912. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  913. PCR Master mix - (reply: 2)
  914. cDNA storage and real time RT PCR - (reply: 3)
  915. Real Time PCR Primers and Probes - (reply: 2)
  916. Anyone make their own PCR cloning vector? - (reply: 5)
  917. Stability of PCR T-overhangs - (reply: 1)
  918. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  919. PCR product wrong size! Need help! - (reply: 9)
  920. pooling different pcr cycles? - (reply: 1)
  921. Amplification of concatenated linear ligated fragments - (reply: 4)
  922. Clinical Real-time PCR assay - (reply: 1)
  923. Need help - How we can design primer for miRNA (reply: 1)
  924. primer and promoter sequences - (reply: 1)
  925. Standard curve for real-time PCR - (reply: 2)
  926. designing of primers - (reply: 2)
  927. Primer Tm differences/ PCR - (reply: 1)
  928. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  929. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  930. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  931. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  932. Designing primer to remove his-tag - (reply: 1)
  933. PCR Primer Dilution from F+R - (reply: 1)
  934. primer reconstitution - primer reconstitution (reply: 4)
  935. Not-Quite-Nested PCR - (reply: 2)
  936. PCR product too short - (reply: 6)
  937. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  938. Primer design for insert amplification - Is this correct? (reply: 2)
  939. got smear on the PCR gel !!! - (reply: 4)
  940. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  941. "Unzipped" PCR band - (reply: 3)
  942. Review on new amplification techniques - (reply: 2)
  943. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  944. DOT BLOT for pcr product - No result obtain (reply: 1)
  945. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  946. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  947. HRM primers - (reply: 1)
  948. PCR amplification with Pfu / quality of DNA - (reply: 4)
  949. primer design - (reply: 2)
  950. Is it possible to compare data between real-time PCR plates - (reply: 1)
  951. Conventional PCR problems - wisamni2004 (reply: 2)
  952. New to sequencing, primer design - (reply: 2)
  953. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  954. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  955. incorrect/smaller band size for real time PCR - (reply: 1)
  956. Quickchange mutagenesis primer - (reply: 3)
  957. pcr / primer theory in bisulfite sequencing - (reply: 4)
  958. homologous amplicon for real time pcr - (reply: 1)
  959. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  960. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  961. assistance with PCR amplification please - (reply: 3)
  962. I need an urgent help with q-pcr amplification plots - (reply: 11)
  963. Problems with the Specificity of the Primers - (reply: 2)
  964. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  965. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  966. Degenerate primers PCR problem, Please help! - (reply: 2)
  967. Hotstart PCR and unspecific Amplification - (reply: 3)
  968. Primer design help needed - (reply: 1)
  969. Primers no longer work - (reply: 1)
  970. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  971. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  972. RT PCR for known snps detection? - SNPs detection (reply: 1)
  973. colony PCR - (reply: 2)
  974. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  975. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  976. PCR reaction without template gives a product - (reply: 6)
  977. PCR wrong product size - (reply: 6)
  978. Wrong pcr product size - (reply: 4)
  979. real time PCR machine - (reply: 5)
  980. PCR very faint product - (reply: 1)
  981. Question about principle of PCR - (reply: 3)
  982. PCR amplification before bisulfite conversion - (reply: 6)
  983. 4 question for real time PCR - (reply: 5)
  984. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  985. PCR didn't work - suspect the enzyme (reply: 2)
  986. Find universal primers in vector - any online tool? (reply: 6)
  987. Picking primers to confirm Illumina meth27 results - (reply: 2)
  988. Colony PCR Specificity - (reply: 2)
  989. Colony Pcr - primers - (reply: 5)
  990. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  991. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  992. Primer Dilution Problem - D'oh! (reply: 2)
  993. Using PCR to create overhangs - (reply: 3)
  994. Creating overhangs with PCR - (reply: 4)
  995. Problem with PCR on bacmids - (reply: 1)
  996. Is there any software that can be sued to design degenerate primers - (reply: 1)
  997. Running real time pcr product on gel?.. - (reply: 4)
  998. Primer Decontamination - (reply: 2)
  999. PCR mutagenesis of plasmid - (reply: 5)
  1000. tools for checking non-specific binding of primers -
    (reply: 1)
  1001. Primer designed for ARMS PCR - (reply: 2)
  1002. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  1003. methylation specific PCR - Primers - (reply: 1)
  1004. bacterial identification using real time PCR - (reply: 1)
  1005. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1006. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1007. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1008. colony PCR for subcloning gene - (reply: 1)
  1009. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1010. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1011. PCR reamplification - (reply: 2)
  1012. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1013. How many colonies to screen (colony PCR)? - (reply: 4)
  1014. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1015. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1016. question about pcr - (reply: 3)
  1017. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1018. How to construct a standard curve for real time PCR - (reply: 1)
  1019. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1020. PCR with one primer - (reply: 1)
  1021. colony PCR inconsistent - (reply: 4)
  1022. PCR contamination - (reply: 19)
  1023. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1024. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1025. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1026. PCR not working - No amplification - (reply: 5)
  1027. Quantification of PCR products - (reply: 1)
  1028. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1029. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1030. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1031. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1032. Nested PCR question - (reply: 1)
  1033. High Tm on primers - cannot get a product - (reply: 8)
  1034. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1035. undesired products in multiplex PCR - (reply: 3)
  1036. NCBI primer blast problem - (reply: 1)
  1037. Help with Multiplex Nested PCR - (reply: 2)
  1038. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1039. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1040. primer sequence problem - (reply: 2)
  1041. Finding bisulfite PCR primer sequence - (reply: 3)
  1042. PCR -No band formation - (reply: 1)
  1043. PCR - No Band formation - (reply: 3)
  1044. No amplification after bisulfite treatment - (reply: 4)
  1045. PCR AMPLIFICATION - (reply: 1)
  1046. Ligation of Blunt PCR Product - (reply: 1)
  1047. real time pcr need advise - (reply: 2)
  1048. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1049. PCR & Cloning - (reply: 6)
  1050. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1051. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1052. DNA pooling for PCR - Saving money PCR (reply: 7)
  1053. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1054. PCR need some help - (reply: 4)
  1055. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1056. Left polymerase out overnight - Will it still work? (reply: 1)
  1057. Primer Reconstitution--does temperature matter? - (reply: 5)
  1058. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1059. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1060. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1061. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1062. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1063. no PCR product from input... - (reply: 8)
  1064. MSP - unwanted amplification - (reply: 5)
  1065. Smears in Bisulfite seq. PCR - (reply: 3)
  1066. Primer design for qPCR - (reply: 2)
  1067. Primer design for qPCR - (reply: 1)
  1068. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1069. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1070. Multiple bands after bisulfite PCR - (reply: 7)
  1071. PCR genomic DNA of high GC content - (reply: 6)
  1072. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1073. primer design tips - (reply: 3)
  1074. PCR with Biotin Incorporation - (reply: 2)
  1075. pcr product - hello all (reply: 1)
  1076. design pcr primer - (reply: 1)
  1077. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1078. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1079. PCR with long and complex primers - (reply: 2)
  1080. Phospholylation of primers - (reply: 1)
  1081. apoptosis by PCR? - (reply: 12)
  1082. Re-using PCR plates? - (reply: 2)
  1083. Touchdown PCR issues - (reply: 3)
  1084. LINE1 OFR2 Primer Design - (reply: 1)
  1085. phosphorylation of primers using PNK - (reply: 1)
  1086. RT-PCR contamination issue - (reply: 3)
  1087. Primers for yeast (18S) - (reply: 1)
  1088. How to reduce the bisulfite PCR bias? - (reply: 4)
  1089. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1090. Primer Concentration to lower Ct - (reply: 1)
  1091. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1092. Primer Concentration Help - (reply: 4)
  1093. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1094. Primer Degradation - What exactly happens? (reply: 1)
  1095. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1096. PCR/DNA Extraction Problem - (reply: 5)
  1097. Primers in 2 exons - (reply: 2)
  1098. NTC appear in real time pcr - (reply: 6)
  1099. Problems with designing a primer - (reply: 2)
  1100. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1101. PCR of AT-rich DNA - (reply: 1)
  1102. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1103. Overlapping PCR - really need help ! - (reply: 5)
  1104. Freezing PCR product??? - (reply: 1)
  1105. problem with pcr cloning from mouse cDNA - (reply: 2)
  1106. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1107. PCR with Platinum Taq - product yield issues - (reply: 3)
  1108. real time PCR - (reply: 7)
  1109. Different sized product for same BS primer! - (reply: 2)
  1110. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1111. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1112. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1113. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1114. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1115. primers with restriction site - (reply: 1)
  1116. PCR product appear two close band in my gel - (reply: 15)
  1117. The Case of a Missing Band: PCR Issue - (reply: 4)
  1118. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1119. cDNA as PCR template - (reply: 1)
  1120. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1121. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1122. paranormal qpcr amplification activity - melting curves (reply: 3)
  1123. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1124. PCR template concentration - (reply: 6)
  1125. Bands in PCR negative control - (reply: 3)
  1126. PCR efficiency calculated by Linreg - (reply: 3)
  1127. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1128. primer design by generunner DG - (reply: 1)
  1129. degenerate pcr - (reply: 1)
  1130. BSP primers design, help please - (reply: 2)
  1131. primers with fluoresent dye PCR cycle question - (reply: 4)
  1132. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1133. Smears on PCR products - (reply: 4)
  1134. Dumb Reverse Transcription PCR Question - (reply: 2)
  1135. Reverse transcription PCR - (reply: 1)
  1136. PCR/RT-PCR beads - (reply: 2)
  1137. High Amplification Efficiency in Std. Curve - (reply: 5)
  1138. Non specific products in Bisulphite pcr - (reply: 4)
  1139. Run PCR amplifications in agarose gels - (reply: 3)
  1140. Problems with validating primers and low expression genes - (reply: 3)
  1141. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1142. Bisulfite sequencing PCR not working - (reply: 5)
  1143. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1144. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1145. intron/exon spanning primers - (reply: 1)
  1146. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1147. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1148. PCR product for sequencing - sample storage?? (reply: 2)
  1149. Absolute quantification using Real Time PCR - (reply: 2)
  1150. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1151. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1152. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1153. MSP primer troubles - (reply: 3)
  1154. Measuring global methylation using real time PCR - (reply: 2)
  1155. Designing Primers for multiple Isoforms - (reply: 1)
  1156. RT-PCR standard curve dilutions - (reply: 2)
  1157. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1158. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1159. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1160. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1161. PCR product dimer issue - (reply: 24)
  1162. PCR additives Formamide - Formamide which one? (reply: 4)
  1163. PCR Master mix - (reply: 1)
  1164. Addition of Restriction sites into PCR primers - (reply: 4)
  1165. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1166. failure PCR amplification from low GC content gene - (reply: 5)
  1167. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1168. conventional v real-time PCR applications - (reply: 1)
  1169. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1170. PCR reagents, can I use reagents from different manufacturers? - (reply: 4)
  1171. different PCR primers for real-time and classic PCR - (reply: 3)
  1172. primers deconamination??? - (reply: 6)
  1173. PCR band too thick - PCR troubleshooting question (reply: 5)
  1174. RT-PCR problem - (reply: 1)
  1175. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1176. primer check!!! - (reply: 6)
  1177. PCR bands on gel electrophoresis - (reply: 5)
  1178. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1179. long range PCR - (reply: 2)
  1180. Annealing Temperature of biotinylated primers - (reply: 2)
  1181. RT-PCR - New to RT-PCR info (reply: 2)
  1182. PCR punch - (reply: 4)
  1183. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1184. PCR off plasmid for screening - (reply: 2)
  1185. PCR failed no product.. help - (reply: 10)
  1186. number of molecules in PCR - (reply: 3)
  1187. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1188. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1189. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1190. Primer dilution - PCR primer dilution (reply: 2)
  1191. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1192. Can't re-PCR the PCR product - (reply: 5)
  1193. PCR reagent autoclave - (reply: 2)
  1194. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1195. Long Primers for PCR - (reply: 2)
  1196. Data Analysis for Real-time PCR - (reply: 2)
  1197. PCR not working - overhangs too long? (reply: 3)
  1198. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1199. argh more pcr headaches! - (reply: 6)
  1200. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1201. Chlamydia PCR cycling conditions - (reply: 1)
  1202. Primers going "loopy" - (reply: 2)
  1203. Conversion of primer unit - ug/ul to uM (reply: 4)
  1204. mutagenesis by PCR or just use a kit - (reply: 5)
  1205. Unsuccessful BS PCR - (reply: 3)
  1206. Primer Trouble Shooting - (reply: 5)
  1207. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1208. Smearing on gel in PCR products - (reply: 5)
  1209. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1210. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1211. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1212. Relative RT-PCR - (reply: 2)
  1213. Relative real time RT PCR - (reply: 2)
  1214. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1215. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1216. a simple primer problem - (reply: 5)
  1217. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1218. no or poor amplification - (reply: 1)
  1219. Primers from 3'UTR - (reply: 2)
  1220. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1221. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1222. Designing primers with ESTs - (reply: 2)
  1223. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1224. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1225. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1226. Real Time vs Traditional PCR results - (reply: 1)
  1227. The problems to identify mouse genotype with PCR - (reply: 1)
  1228. addition of BclI restriction site to PCR primer - (reply: 6)
  1229. non specific amplification - DNA amplification by PCR (reply: 1)
  1230. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1231. How does methprimer calculate primer Tm - (reply: 2)
  1232. Laminar Flow vs PCR cabinet - (reply: 4)
  1233. How to do PCR to detect mRNA without RT? - (reply: 2)
  1234. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1235. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1236. PCR has stopped working - (reply: 7)
  1237. PCR negative control contamination - (reply: 8)
  1238. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1239. Phosphorylated primers - (reply: 5)
  1240. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1241. PCR efficiency in real timePCR - (reply: 5)
  1242. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1243. Drastic Decrease in PCR product yield - (reply: 2)
  1244. resources on PCR principles & technique - (reply: 1)
  1245. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1246. Colony Screeing without using PCR - (reply: 8)
  1247. High MW PCR band seen...help needed - (reply: 7)
  1248. QPCR - Primer and Probe question - QPCR (reply: 2)
  1249. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1250. standard curve/ PCR efficiency problems... - (reply: 1)
  1251. Help! PCR that used to work doesn't work now! - (reply: 2)
  1252. PCR and templates - (reply: 3)
  1253. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1254. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1255. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1256. Easy primer question? - Primers (reply: 1)
  1257. PCR inconsistency - (reply: 4)
  1258. Plasmid problem - From PCR product (reply: 7)
  1259. help needed: PCR a gene from genomic DNA - (reply: 2)
  1260. questions about pcr products after pooling - (reply: 3)
  1261. stiching/linking/sewing pcr - (reply: 3)
  1262. Human mtDNA amplification problems - (reply: 3)
  1263. primer dimer - proplems (reply: 1)
  1264. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1265. Nested PCR - (reply: 2)
  1266. pfu vs long pcr mix - (reply: 7)
  1267. weird PCR ask for help - (reply: 12)
  1268. Methylation specific DMSO PCR - (reply: 1)
  1269. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1270. No PCR product at all - (reply: 8)
  1271. PCR troubleshoooooting - primer dimer (reply: 1)
  1272. Primer efficiency test - (reply: 1)
  1273. primer software - (reply: 2)
  1274. Question on wired PCR - (reply: 1)
  1275. restriction digestion of PCR product - (reply: 7)
  1276. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1277. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1278. How to know in which exon a primer match? - (reply: 1)
  1279. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1280. Amplification of human genomic DNA - (reply: 2)
  1281. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1282. sequencing with forward/reverse primers - (reply: 7)
  1283. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1284. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1285. Adding A overhangs - primer design implications? - (reply: 1)
  1286. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1287. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1288. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1289. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1290. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1291. primer design problem - (reply: 1)
  1292. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1293. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1294. Problem with primer efficiency analysis - (reply: 4)
  1295. slan real time PCR system for validation of microarray results? - (reply: 1)
  1296. PCR unusualband at ~230bps - PCR (reply: 1)
  1297. DMSO or BSA for PCR - (reply: 6)
  1298. Whole Genome Amplification - (reply: 2)
  1299. primer design - (reply: 1)
  1300. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1301. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1302. designing primers for genes not sequenced yet - (reply: 2)
  1303. Tris Buffer for PCR reaction - why? (reply: 1)
  1304. PCR a plasmid protein - (reply: 2)
  1305. multiplex PCR - (reply: 4)
  1306. Realtime PCR machine - (reply: 2)
  1307. PCR set-up calculation nightmares. - (reply: 3)
  1308. Primer desing - (reply: 2)
  1309. Colony PCR - (reply: 3)
  1310. Standard curves for PCR efficiency. - (reply: 2)
  1311. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1312. Question about RAPD PCR - (reply: 2)
  1313. Looking for help with my PCR! - (reply: 3)
  1314. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1315. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1316. primer design - (reply: 9)
  1317. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1318. PCR without thermal cycler? - (reply: 9)
  1319. primer contamination - primer contaminated with ice (reply: 3)
  1320. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1321. RT-PCR problem - (reply: 4)
  1322. Only DNA ladder , No desired band in PCR - (reply: 4)
  1323. taq and PCR - (reply: 6)
  1324. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1325. PCR Efficiency - (reply: 2)
  1326. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1327. Long PCR and genomic DNA isolation problems - (reply: 2)
  1328. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1329. NTC with specific amplification - (reply: 1)
  1330. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1331. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1332. Primer3 vs Primer BLAST - (reply: 3)
  1333. PCR and then ligation - (reply: 7)
  1334. Primer design: free energy - (reply: 2)
  1335. Amplification in NTC and noRT controls - (reply: 5)
  1336. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1337. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1338. asymmetric PCR - (reply: 1)
  1339. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1340. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1341. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1342. trouble with pcr - (reply: 8)
  1343. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1344. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1345. Real time PCR results-interpretation - (reply: 1)
  1346. Real time PCR results-interpretation - (reply: 2)
  1347. Primer optimization for ChIP - (reply: 2)
  1348. sequencing primer: T7 or T7promoter? - (reply: 4)
  1349. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1350. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1351. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1352. primer tm - tm calculation for tagged primers (reply: 2)
  1353. Reusing 96 well plates for PCR - (reply: 2)
  1354. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1355. PCR and AFLP - (reply: 1)
  1356. Universal 16s rRNA primers needed - (reply: 3)
  1357. Identifying PCR Inhibitors - (reply: 10)
  1358. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1359. PCR + phusion enzyme = massive errors - (reply: 4)
  1360. long DNA amplification - (reply: 11)
  1361. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1362. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1363. adding koazak sequence to primer - (reply: 1)
  1364. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1365. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1366. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1367. Primer design if sequence is unknown for organism - (reply: 5)
  1368. adding C terminal tag to reverse primer - (reply: 1)
  1369. epitope tagging of pcr products - (reply: 1)
  1370. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1371. Real time PCR info - (reply: 4)
  1372. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1373. PCR from BAC - PCR from BAC (reply: 1)
  1374. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1375. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1376. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1377. How much product PCR?? - (reply: 3)
  1378. what primers should I use for DNA sequencing? please help - (reply: 2)
  1379. what primers should I use for DNA sequencing?? - (reply: 3)
  1380. Is my primer going to work for qRT-PCR? - (reply: 6)
  1381. Standard curve for RT-PCR - (reply: 1)
  1382. PCR using genomic DNA - (reply: 2)
  1383. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1384. Problem amplifying plasmid - (reply: 1)
  1385. Sybr Green I in real-time PCR reaction - (reply: 3)
  1386. Primers annealing temperatures - (reply: 8)
  1387. Difficulties cloning by PCR - (reply: 5)
  1388. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1389. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1390. high Cp value PCR - (reply: 2)
  1391. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1392. Synthesize own Oligo-dT primers - (reply: 6)
  1393. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1394. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1395. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1396. Smearing/ no amplification in PCR - (reply: 3)
  1397. primer design - Primer design for PCR (reply: 1)
  1398. How are primers designed? - (reply: 4)
  1399. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1400. DNA methylated PCR - (reply: 2)
  1401. need help on primer calcuation soon - (reply: 1)
  1402. primer design - (reply: 6)
  1403. problem in pcr and purification - (reply: 3)
  1404. Inability to duplicate PCR results - (reply: 8)
  1405. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1406. PCR using xt5 - (reply: 2)
  1407. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1408. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1409. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1410. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1411. 23S rRNA Primer - need primer sequence (reply: 1)
  1412. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1413. RT-PCR - problems with TaqMan PCR (reply: 2)
  1414. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1415. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1416. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1417. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1418. Small product amplification problems - (reply: 4)
  1419. multi way ligation or long range pcr? - (reply: 1)
  1420. Freezing primers+SYBR green - (reply: 2)
  1421. Tagged Primers - (reply: 5)
  1422. no pcr amplification product - (reply: 9)
  1423. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1424. design primer for chip - (reply: 5)
  1425. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1426. PCR problem - (reply: 4)
  1427. differently behavioring replicates in real-time PCR - (reply: 1)
  1428. Multiplex PCR - (reply: 1)
  1429. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1430. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1431. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1432. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1433. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1434. PCR components storage - (reply: 3)
  1435. reconstitution of primers - (reply: 2)
  1436. No PCR bands with some templates - (reply: 5)
  1437. Mysterious PCR Contamination - (reply: 13)
  1438. BS PCR..please help - DNA methylation analysis (reply: 15)
  1439. RT-PCR Help - Protocol provided (reply: 4)
  1440. PCR product purification - (reply: 3)
  1441. Mutagenesis PCR and undesired amplification - (reply: 2)
  1442. Temp Gradient PCR - (reply: 2)
  1443. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1444. PCR with genomic dna - (reply: 9)
  1445. primer dimer - (reply: 2)
  1446. PCR with long primers - (reply: 3)
  1447. BSP PCR standardization...help - (reply: 8)
  1448. PCR on degraded templates - (reply: 2)
  1449. PCR help needed - (reply: 3)
  1450. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1451. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1452. light bands, dark smear, dark dimers - (reply: 2)
  1453. PCR, followed by sequencing... - why ?? (reply: 3)
  1454. PCR program - strange extension step (reply: 2)
  1455. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1456. Help me! How to design nested BSP primers? - (reply: 4)
  1457. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1458. Plasmid linearization by PCR - (reply: 4)
  1459. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1460. Using BLAST to check primers - (reply: 17)
  1461. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1462. pcr doesnt show any band for DNa walking - (reply: 8)
  1463. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1464. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1465. Effects of more cDNA in PCR??? - (reply: 1)
  1466. PCR primer - (reply: 2)
  1467. PCR product disappears after restriction digestion - (reply: 4)
  1468. Primer design - GC clamp - (reply: 8)
  1469. Real Time PCR - excluding Ct - (reply: 2)
  1470. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1471. Price of a real time PCR machine? - (reply: 4)
  1472. colony PCR - (reply: 8)
  1473. probe conc. of Asymmetric PCR - (reply: 1)
  1474. primer dimer or what? - (reply: 2)
  1475. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1476. Real Time PCR method comments - (reply: 2)
  1477. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1478. colony pcr - (reply: 4)
  1479. Primers and Taqman Probes mixture question - (reply: 1)
  1480. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1481. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1482. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1483. Colony PCR - (reply: 15)
  1484. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1485. no bands in my PCR - (reply: 9)
  1486. designing primers - (reply: 1)
  1487. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1488. purefication and amplification of serum free DNA - (reply: 1)
  1489. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1490. Bisulfite Sequencing PCR - (reply: 4)
  1491. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1492. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1493. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1494. mRNA PCR - (reply: 1)
  1495. pcr product quantification - (reply: 5)
  1496. Negative Control Primers for ChIP Assay - (reply: 3)
  1497. Magnesium in PCR - (reply: 1)
  1498. PCR Sample Prep - (reply: 4)
  1499. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1500. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1501. Bizarre Contamination in PCR - (reply: 7)
  1502. PCR Cloning-large primers - (reply: 4)
  1503. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1504. forward and reverse primer - (reply: 4)
  1505. Check the primers - (reply: 4)
  1506. PCR problem from transformed TOPO TA vector - (reply: 3)
  1507. DIG DNA PCR labeling problem - (reply: 6)
  1508. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1509. BSP primers - (reply: 2)
  1510. Various size of insert after colony screen by PCR - (reply: 2)
  1511. inverse PCR molecular bilogy - (reply: 3)
  1512. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1513. failed PCR on DNA extract from blood - (reply: 6)
  1514. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1515. Primer dimer issue in real time PCR - (reply: 21)
  1516. Leaky RT-PCR - (reply: 4)
  1517. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1518. PCR, RNA, Northern Blotting???? - (reply: 1)
  1519. bizzare PCR smear - why does science hate me (reply: 7)
  1520. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1521. PCR detection of SNP - (reply: 6)
  1522. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1523. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1524. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1525. Sub-cloning sticky-end PCR products - (reply: 2)
  1526. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1527. ChIP on chip amplification problems - (reply: 11)
  1528. Genotyping PCR - (reply: 3)
  1529. PCR screening of transformed bacterial colony - (reply: 6)
  1530. Is this standard valid? - pcr product as standards (reply: 5)
  1531. primer binding - (reply: 1)
  1532. weird ChIP primers! - (reply: 1)
  1533. PCR primers - (reply: 1)
  1534. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1535. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1536. amplification in negative control in RT PCR - (reply: 2)
  1537. miRNA real time PCR by SYBR green methods - (reply: 1)
  1538. Deletion PCR - (reply: 9)
  1539. diluting to final primer concentration - please help correct (reply: 1)
  1540. Smear problem with my new primer set - (reply: 2)
  1541. PCR ghost bands - (reply: 1)
  1542. Amplifying a gene using a degenerate primer - (reply: 4)
  1543. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1544. CP of real time PCR by LC480 - (reply: 1)
  1545. CHIP- Real Time PCR calculations - (reply: 1)
  1546. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1547. transgene copy number - using real time PCR (reply: 1)
  1548. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1549. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1550. Unspecific PCR - (reply: 7)
  1551. Real-Time PCR as a Microplate Reader - (reply: 1)
  1552. gene-specific RT-PCR - (reply: 4)
  1553. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1554. RT-PCR Internal Standard - (reply: 2)
  1555. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1556. Plasmid supercoiling affecting PCR? - (reply: 2)
  1557. RNA contamination in PCR - (reply: 1)
  1558. PCR without DNA extraction! - (reply: 2)
  1559. Primer tm - (reply: 1)
  1560. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1561. re-amplification in real-time PCR - (reply: 1)
  1562. primer design - really necessary? (reply: 2)
  1563. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1564. RT-PCR - (reply: 3)
  1565. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1566. Degenerative primers = multiple products? - (reply: 5)
  1567. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1568. PCR problems on a ligation product - (reply: 5)
  1569. Genomic DNA and PCR - (reply: 4)
  1570. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1571. Sewing PCR - (reply: 3)
  1572. on the minimal pcr derived product - (reply: 2)
  1573. primer dimers - (reply: 5)
  1574. RT-PCR vs. conventional PCR - (reply: 1)
  1575. qPCR primer design - (reply: 5)
  1576. PCR and gel purification problem - did I screw this up (reply: 4)
  1577. Cloning PCR fragment - (reply: 1)
  1578. touchdown pcr - (reply: 6)
  1579. non specific pcr products - (reply: 4)
  1580. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1581. Anyone with experiences in 2 step PCR - (reply: 1)
  1582. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1583. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1584. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1585. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1586. re-amplification of a PCR product - is it recommended? (reply: 7)
  1587. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1588. PCR machine with accurate temperature control? - (reply: 1)
  1589. Gene-specific RT-PCR - (reply: 2)
  1590. Primer stock confusion - (reply: 6)
  1591. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1592. primer design - primer design question (reply: 2)
  1593. Protocol for 16S rDNA real time PCR - (reply: 2)
  1594. Touchdown PCR - (reply: 1)
  1595. Determine annealing temperature of primers - (reply: 14)
  1596. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1597. polymerase for BSP - (reply: 2)
  1598. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1599. Validating real time pcr primers - (reply: 2)
  1600. BSP,MSP primer design? - (reply: 1)
  1601. low gc primers - help with pcr using low gc primers (reply: 6)
  1602. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1603. Primers and annealing temperature - (reply: 2)
  1604. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1605. Copy number calculations in real time PCR - (reply: 2)
  1606. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1607. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1608. RT-PCR Negative controls - (reply: 1)
  1609. WHich primer to use for sequencing - (reply: 5)
  1610. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1611. minimum length for the gene to be amplified in PCR - (reply: 6)
  1612. dNTP Quantity - (reply: 3)
  1613. annoying PCR cloning problem (season 2) - (reply: 5)
  1614. PCR with 60 bp primers - results in no product (reply: 6)
  1615. Amplification dwindles using little template RT-PCR - (reply: 3)
  1616. Running gel after RT-PCR - (reply: 5)
  1617. Degenerate PCR Size Limit? - (reply: 3)
  1618. primer design for pBAD topo expression kit - (reply: 2)
  1619. PCR problems.. - problems with conventional PCR (reply: 3)
  1620. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1621. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1622. small PCR products on agarose gel? - (reply: 8)
  1623. Dnase digestion and PCR - (reply: 4)
  1624. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1625. primers fpr sequencing - (reply: 4)
  1626. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1627. Need an UV260 RT-PCR instrument - (reply: 1)
  1628. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1629. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1630. PCR produces products of wrong size - (reply: 4)
  1631. PCR with pfu and degenerate primers (?) - (reply: 7)
  1632. a doubt about PCR gel purification - (reply: 4)
  1633. Draw PCR primer locations - PCR primer (reply: 4)
  1634. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1635. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1636. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1637. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1638. PCR+enhancer - (reply: 4)
  1639. Serious issues with RT-PCR - (reply: 7)
  1640. PCR [dNTP] - dNTP concentrations (reply: 2)
  1641. PCR issue - (reply: 2)
  1642. dNTP question - (reply: 3)
  1643. PCR - (reply: 1)
  1644. long PCR primer - (reply: 7)
  1645. Taqman rtPCR primer and probe design - (reply: 3)
  1646. sequencing problem after pcr - (reply: 4)
  1647. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1648. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1649. primer design - (reply: 3)
  1650. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1651. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1652. Analysis of ChIP RT-PCR data - (reply: 7)
  1653. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1654. Negative flouroscence in real time PCR - (reply: 2)
  1655. Minimum number of PCR cycles to see a product? - (reply: 6)
  1656. decontamination - decontamination for PCR (reply: 6)
  1657. Troubleshoot ARMS PCR - (reply: 2)
  1658. negative control for PCR - (reply: 1)
  1659. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1660. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1661. pcr pdt amplification - (reply: 1)
  1662. real time PCR - (reply: 3)
  1663. PCR fails when I scale up - (reply: 2)
  1664. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1665. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1666. PCR not Working = SMEAR - (reply: 3)
  1667. How to do PCR fragment in 2 steps - (reply: 3)
  1668. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1669. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1670. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1671. RT-PCR - problem of PCR (reply: 1)
  1672. PCR product longer than template - why? - (reply: 2)
  1673. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1674. Dye recipe that can be used for PCR MM - (reply: 3)
  1675. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1676. PCR conditions with three primers - (reply: 1)
  1677. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1678. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1679. Cloning a labeled PCR product - (reply: 1)
  1680. PCR and cloning - (reply: 14)
  1681. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1682. PCR product - not suppose to be there (reply: 7)
  1683. using the meth primer - (reply: 2)
  1684. Generating primers for MSP - (reply: 1)
  1685. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1686. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1687. PCR from a smear genomic DNA ? - (reply: 5)
  1688. Primer design and need help - (reply: 11)
  1689. Setting up a multiplex PCR assay. - (reply: 2)
  1690. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1691. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1692. cDNA and RT-PCR - (reply: 8)
  1693. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1694. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1695. PCR problem - help me plsss (reply: 9)
  1696. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1697. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1698. PCR efficiencies - (reply: 4)
  1699. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1700. PCR quantification - (reply: 1)
  1701. PCR quantification - (reply: 1)
  1702. Long PCR product - (reply: 9)
  1703. problem in Pcr amplification - (reply: 7)
  1704. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1705. difference between hotstart and taq polymerase - (reply: 7)
  1706. sequencing the pcr product - (reply: 15)
  1707. Pcr amplification - primer designed from UTR regions (reply: 5)
  1708. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)