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PCR Related Discussions
  1. High molecular weight band from my PCR - (reply: 3)
  2. Can someone help me on my primer design? (contains flag tag) - (reply: 2)
  3. testing primers efficiency for rt-PCR - (reply: 1)
  4. -RT contamination problem in PCR - (reply: 5)
  5. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
  6. Thermal Cycler for Multiplex PCR - (reply: 3)
  7. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
  8. Primer sequences with M and Y - how to order? - (reply: 2)
  9. RT-PCR low efficiency - (reply: 1)
  10. emulsion PCR - (reply: 1)
  11. RT-PCR electrophorasis - (reply: 1)
  12. PCR contamination - (reply: 6)
  13. PCR bands on gel - (reply: 5)
  14. Problem with restriction digestion of cloned PCR product - (reply: 5)
  15. SYBR Green Real time PCR - (reply: 2)
  16. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
  17. need help calculating stock primer concentration - (reply: 1)
  18. High background fluorescence detected before starting PCR - (reply: 3)
  19. How to clean up PCR place & pipettes? - (reply: 1)
  20. Real-time pcr - (reply: 1)
  21. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
  22. PCR inhibitors - (reply: 1)
  23. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
  24. Cleaning loading dye for PCR sequencing - (reply: 1)
  25. Polymerase mixture for blunt ended fragments - (reply: 2)
  26. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  27. Amplifying shRNA lentivirus - (reply: 1)
  28. Mineral oil quality in PCR tubes - (reply: 5)
  29. Diagnostic PCR troubleshooting - (reply: 2)
  30. RT-PCR Kit's Validation - (reply: 1)
  31. Rt pcr - (reply: 4)
  32. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
  33. methylation specifc primers thermal cycle conditions - (reply: 4)
  34. PCR Amplification Issues and Primer dimer - (reply: 4)
  35. How to make PCR analysis using the 22DDCT Method - (reply: 1)
  36. Primer calculation for qPCR - final reaction - (reply: 1)
  37. Sec. structure in primers - (reply: 1)
  38. Changing PCR recipe halfway - (reply: 1)
  39. PCR master mix containing primers and Taq - (reply: 4)
  40. Primer working with some cDNA samples and not other - (reply: 2)
  41. no bands after pcr!!!! - (reply: 4)
  42. Experimental design for RT-PCR - (reply: 8)
  43. Primer dilution - (reply: 7)
  44. why not working msp primers - (reply: 4)
  45. PCR product not migrating from wells...?? - (reply: 6)
  46. Re-PCR my PCR products - (reply: 2)
  47. PCR DIG-labeling kit preblem? - (reply: 1)
  48. pcr - (reply: 1)
  49. how to analysis the BiSearch ePCR result - (reply: 3)
  50. Standardizing cDNA concentration before doing PCR - (reply: 4)
  51. Volume of Primers - (reply: 4)
  52. PCR conditions unknown? - (reply: 10)
  53. PCR anomaly in a mid-range dilution of the template - (reply: 2)
  54. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
  55. MSP_Primer dimers? - (reply: 17)
  56. PCR using very long oligos !!! - (reply: 4)
  57. Primer Designing - (reply: 9)
  58. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  59. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  60. colony pcr, two strong bands - (reply: 4)
  61. Ligation of two PCR products - (reply: 1)
  62. pcr primer design - (reply: 6)
  63. Please help (primer/ probe final volume calculations) - (reply: 1)
  64. Primer design with EcoRI ends - (reply: 2)
  65. Amplification problem in DNA isolated after ChIP experiment, - (reply: 1)
  66. Trouble with PCR using ligation mix as template? - (reply: 3)
  67. PCR with mammalian cells as template - (reply: 1)
  68. PCR with overlapping primers only (primer extention) - (reply: 3)
  69. Random chuck of nonsense in middle of PCR - (reply: 6)
  70. RT-PCR analysis - (reply: 1)
  71. PCR DNA bands too large on agarose gel - (reply: 1)
  72. MSP Primer design - (reply: 5)
  73. New to PCR - (reply: 3)
  74. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  75. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
  76. Primer design - (reply: 1)
  77. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)
  78. Primer non specific binding sites - (reply: 7)
  79. Degenerate primers - (reply: 8)
  80. Primers with 2 different annealing temp - (reply: 1)
  81. Can you use same samples for real time pcr 2nd time? - (reply: 2)
  82. Staphylococcus aureus colony PCR - (reply: 1)
  83. Cloning purified PCR product into cells - (reply: 5)
  84. PCR & Western Blot sample preperation - (reply: 2)
  85. PCR & Western Blot sample preperation - (reply: 5)
  86. Y-linker transgene integration site PCR primer verification - (reply: 1)
  87. Non especific band in PCR... - (reply: 2)
  88. I need help with PCR problems - (reply: 5)
  89. Question about baselines in Real-Time PCR - (reply: 3)
  90. Inconsistent colony PCR result - (reply: 2)
  91. A problem with PCR array - (reply: 4)
  92. My PCR suddenly stopped working and I'm losing my mind - (reply: 3)
  93. Concentration of Eva green with different amplicon sizes in a PCR pool - (reply: 1)
  94. labX offers on realtime PCR machines - (reply: 4)
  95. Could any of you help with an explanation to these vague real time PCR curves &# - (reply: 2)
  96. polymerase with or without proofreading activity used in study nucleotide polymo - (reply: 1)
  97. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  98. Suggest best micropipette for PCR purpose... - (reply: 1)
  99. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  100. Plasmid Amplification Issue - (reply: 6)
  101. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  102. primer design for a gene - (reply: 3)
  103. large bands in all pcr reactions including negative control - (reply: 1)
  104. confirmation for primers dilution - (reply: 10)
  105. Problems regarding amplification of my gene - (reply: 3)
  106. PCR working, qPCR is *not*. - (reply: 6)
  107. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  108. Overlapping PCR, need help - (reply: 5)
  109. How does RT-PCR work? - (reply: 5)
  110. Denatured plasmid for pcr - (reply: 1)
  111. Possible primer dimer problem..Need solution!!! - (reply: 2)
  112. confusing with where to put primers and which ordination - (reply: 4)
  113. Help with primer concentration for sequencing - (reply: 1)
  114. PCR troubleshoot - (reply: 6)
  115. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  116. PCR Products not run well in the GEL - (reply: 1)
  117. PCR solution calculus - (reply: 4)
  118. Amplifying from pcr products - (reply: 7)
  119. Consistancy issue with PCR block - (reply: 1)
  120. Queries regarding PCR - (reply: 3)
  121. Design the primers - (reply: 10)
  122. Calculation of Taq Polymerase - (reply: 3)
  123. Best Taq for colony PCR? - (reply: 5)
  124. How two fragments joint together when doing fusion PCR? - (reply: 4)
  125. How to test my milleq water in q PCR about contaminants - (reply: 3)
  126. Mutagenesis PCR - (reply: 9)
  127. real time PCR primer design - (reply: 1)
  128. The PCR gods are frowning upon me - (reply: 9)
  129. PCR after T4 ligation? - (reply: 3)
  130. primers for cloning - (reply: 1)
  131. Degenerated Primers and their problems - (reply: 1)
  132. Sonication of small PCR amplicon - (reply: 1)
  133. primer design with restriction enzyme - (reply: 1)
  134. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  135. primer dilution help - (reply: 1)
  136. Testing primers on 'unknown' tissue - (reply: 1)
  137. How to remove inhibitory substances in PCR? - (reply: 2)
  138. need some advise about PCR of PKD1-human gene - (reply: 7)
  139. puzzled with PCR outcome after BS treatment - (reply: 2)
  140. nested PCR for low viral load- HBV patient sample - (reply: 2)
  141. troubleshooting stubborn PCR - (reply: 6)
  142. Measuring pcr fragments in a gel - (reply: 1)
  143. Amplification in HK gene but not for target gene - (reply: 3)
  144. could you help me with my stem loop RT primer? - (reply: 1)
  145. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  146. How long can I store at - 30°C my PCR mix? - (reply: 6)
  147. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  148. RT-PCR help - (reply: 2)
  149. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  150. PCR Temperature change control malfunctioning - (reply: 1)
  151. excess amount of primers - (reply: 2)
  152. Dehydrating and Reconstituting primers - (reply: 15)
  153. Trouble with PCR of short sequence - (reply: 3)
  154. Setting the temperature range for gradient PCR - (reply: 3)
  155. KIR gene promoters for MSP primers design - (reply: 2)
  156. Biotin-streptavidin signal amplification - (reply: 2)
  157. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  158. Amplification curve in the negative control samples - (reply: 7)
  159. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  160. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  161. Electrophoresis after PCR : too many bands - (reply: 6)
  162. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  163. Primer as limiting reagent in PCR reaction - (reply: 2)
  164. Primer Design for RNA probes - (reply: 2)
  165. Real time PCR for degraded RNA - (reply: 1)
  166. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  167. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  168. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  169. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  170. Nested BSP Primer Design - (reply: 6)
  171. website for primer design - (reply: 1)
  172. PCR Efficiency over 150%! - (reply: 1)
  173. problem of amplification - (reply: 2)
  174. PCR not working - (reply: 11)
  175. How to do a primer dilution - (reply: 10)
  176. Troubles with Fusion PCR - (reply: 1)
  177. Primer design and alternative transcripts - (reply: 2)
  178. PCR and sequencing of genomic DNA - (reply: 5)
  179. qPCR amplification - (reply: 4)
  180. NESTED PCR - (reply: 6)
  181. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  182. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  183. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  184. Digestion necessary after PCR? - (reply: 9)
  185. Inverse PCR product selection - (reply: 2)
  186. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  187. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  188. how to hasten real-time PCR amplifications - (reply: 2)
  189. Please please help me with my Phusion PCR. - (reply: 5)
  190. PCR - (reply: 1)
  191. NCBI Primer Design - Stringency Issues - (reply: 3)
  192. Different primer concentration in qPCR - (reply: 1)
  193. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  194. help in long pcr - (reply: 1)
  195. PCR inhibitor in template DNA - (reply: 3)
  196. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  197. Problem with Real-time PCR results analysis - (reply: 1)
  198. Primer Specificity: Testing only one primer - (reply: 4)
  199. PCR from protozoa DNA - (reply: 3)
  200. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 7)
  201. tool for comparing many primers pairs - (reply: 4)
  202. PCR that leads to protein synthesis - (reply: 18)
  203. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  204. Use of DMSO in General PCR - (reply: 1)
  205. PCR product size confusion - (reply: 3)
  206. Pcr primers - (reply: 7)
  207. Concentration specification in PCR - (reply: 3)
  208. Guanidine isothiocyanate in PCR - (reply: 1)
  209. Primers have worked well but now getting primer dimers? - (reply: 2)
  210. I cannot design primers on exon-exon junction - (reply: 2)
  211. DNA Quantification of PCR Products - (reply: 2)
  212. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  213. Problem for PCR - (reply: 9)
  214. Designing primers in UTRs - (reply: 1)
  215. Multiplex PCR - (reply: 1)
  216. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  217. primer design@ buy? - (reply: 2)
  218. Trouble with overlap extension pcr - (reply: 3)
  219. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  220. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  221. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  222. Question about the RT PCR - (reply: 3)
  223. faint dna band after pcr purification - (reply: 1)
  224. PCR ready mixes with long shelf lives - (reply: 4)
  225. Troubleshooting: Inverse PCR - (reply: 3)
  226. How to design primer to amplify genomic DNA? - (reply: 3)
  227. Overlap PCR, need help - (reply: 11)
  228. Internal control for miRNA RT-PCR - (reply: 1)
  229. Primers - (reply: 1)
  230. Primers mix - (reply: 2)
  231. question about RNA concentration for real time PCR - (reply: 1)
  232. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  233. PCR products sizes and DNA ladder - (reply: 7)
  234. Having problem with primers for qPCR - (reply: 4)
  235. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  236. PCR product sequencing - (reply: 3)
  237. Bad fragment amplification - (reply: 4)
  238. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  239. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  240. No amplification with TRAPEZE kit! - (reply: 1)
  241. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  242. Stargazer PCR problems - (reply: 3)
  243. General PCR discussion - (reply: 8)
  244. Real time. No amplification but hight flourescence - (reply: 1)
  245. Real time PCR doubt - (reply: 5)
  246. PCR Profile for ligation - (reply: 3)
  247. protocol to relieve melanin inhibition of PCR - (reply: 4)
  248. No bands despite different primers and conditions and TAqs - (reply: 8)
  249. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  250. Equimolar Mix Primer - (reply: 8)
  251. Wrong PCR product - (reply: 2)
  252. Colony PCR positive and Digestion negative????? - (reply: 11)
  253. Primer design - (reply: 5)
  254. storage for lyophilized primers - (reply: 1)
  255. PCR GAPDH gene - (reply: 1)
  256. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  257. Quantitative RT-PCR statistics help - (reply: 1)
  258. Dimerization of PCR product - (reply: 4)
  259. PCR Primer trouble - (reply: 2)
  260. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  261. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  262. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  263. finding the corrosponding primers - (reply: 2)
  264. Gene sequence for Real time PCR - (reply: 2)
  265. non-reproducible PCR results with cDNA as template - (reply: 1)
  266. Ligation of PCR fragments - (reply: 11)
  267. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  268. RNA extraction for RT-PCR - (reply: 3)
  269. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  270. problems with gDNA doing real time PCR in yeast - (reply: 2)
  271. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  272. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  273. How to get rid of bands in PCR negative control - (reply: 10)
  274. T7 and M13 primers two band amplification - (reply: 3)
  275. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  276. Strange PCR problem - (reply: 2)
  277. Question about Double Digestion followed by PCR amplification - (reply: 2)
  278. PCR problem - (reply: 2)
  279. PCR machine not working properly - (reply: 1)
  280. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  281. PCR gene specific amplification problem - (reply: 3)
  282. Failure SYBRGREEN PCR - (reply: 4)
  283. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  284. without RE site in PCR product - (reply: 5)
  285. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  286. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  287. real-time pcr non reproducible - (reply: 4)
  288. Strange amplification plots with high Ct variability - (reply: 1)
  289. How big a role does mixing play in PCR - (reply: 1)
  290. Melting curve is irregular for primer optimization - (reply: 5)
  291. Designing primers for ABO blood groups - (reply: 1)
  292. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  293. RT-PCR primer design - (reply: 7)
  294. How to amplify very short PCR template - (reply: 4)
  295. Is this primer okay? - (reply: 4)
  296. PCR amplification of large template - (reply: 1)
  297. PCR insert - in frame? - (reply: 2)
  298. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  299. smear in gel electrophoresis after PCR - (reply: 2)
  300. Can I use Primer as template? - (reply: 1)
  301. Whole plasmid amplification by PCR - (reply: 2)
  302. PCR product as standard curve template - (reply: 6)
  303. colony PCR after transformation - (reply: 1)
  304. PCR machine - (reply: 3)
  305. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  306. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  307. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  308. Resuspending primers calculation - (reply: 4)
  309. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  310. PCR RFLP is PCR product purification necessary? - (reply: 3)
  311. Mystery in my PCR - (reply: 5)
  312. Whole genome amplification from cDNA? - (reply: 4)
  313. What do you use to check primer secondary structure? - (reply: 3)
  314. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  315. PCR product as template for in vitro transcription - (reply: 1)
  316. Problem with 3 step PCR - (reply: 3)
  317. mutagenic primers with very high GC content. - (reply: 3)
  318. Scaling up PCR to get more DNA - (reply: 5)
  319. PCR to get 10kbp product - (reply: 4)
  320. site-directed mutagenesis primer Tm - (reply: 4)
  321. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  322. Random vs oligo primer in preamplification RT - (reply: 1)
  323. how does polymerase stop at the required length - (reply: 1)
  324. Chip pcr. are there inespecifics? - (reply: 1)
  325. Primer concentration - stupid question - (reply: 3)
  326. Primer Design, help i´m New - (reply: 4)
  327. No DNA after PCR product purification - (reply: 9)
  328. Sensitivity of RT-PCR and qPCR - (reply: 4)
  329. RT-qPCR primer problem - (reply: 4)
  330. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  331. real time PCR trouble - (reply: 3)
  332. removal of ethanol in PCR product purification - (reply: 5)
  333. Crazy real time PCR curve - (reply: 4)
  334. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  335. High fidelity PCR trouble shooting - (reply: 2)
  336. Designing cloning primers for DNMT - (reply: 2)
  337. Reproducible Non-Specific PCR Product - (reply: 2)
  338. Are these primer products good enough for qPCR? - (reply: 3)
  339. Can't get PCR with large overhang primers to work - (reply: 8)
  340. Primer Efficiency across runs - (reply: 1)
  341. Question about pipet tips for PCR and rtPCR - (reply: 4)
  342. UNG in PCR - (reply: 1)
  343. Cause of random samples failing PCR? - (reply: 2)
  344. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  345. pcr 10X buffer preparation - (reply: 2)
  346. how much template do i need for pcr? - (reply: 5)
  347. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  348. Problems with crossover PCR - (reply: 2)
  349. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  350. Designing PCR Primers for cloning - (reply: 17)
  351. Creating primers to add restriction sites to vector backbone - (reply: 7)
  352. PCR DNA Concentration - (reply: 1)
  353. RT-PCR product- no band - (reply: 4)
  354. Some primers dose not work - (reply: 5)
  355. Understanding RACE PCR - (reply: 1)
  356. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  357. Wierd Bands after PCR....Confused - (reply: 9)
  358. Query regarding primers for quick change mutagenesis - (reply: 3)
  359. PCR with Plasmid recovered from filter paper - (reply: 6)
  360. PCR amplified product size - (reply: 5)
  361. the storage time for primers - (reply: 9)
  362. Annealing temperature for PCR - (reply: 8)
  363. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  364. Nested PCR - (reply: 2)
  365. random primers or oligodT - (reply: 4)
  366. No amplification during RT-PCR - (reply: 4)
  367. Sequencing RT-PCR product - (reply: 3)
  368. Low yield PCR product after gel purification - (reply: 8)
  369. To design or use published primers? - (reply: 4)
  370. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  371. PCR primer usage (Clonning & cDNA) - (reply: 2)
  372. problem in cloning PCR primer design with restriction site - (reply: 4)
  373. Amplification with U primers, but not with M primers - (reply: 1)
  374. Taq polymerase - (reply: 7)
  375. His tag introduction in to gene and primer design - (reply: 2)
  376. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  377. Which High-Fidelity polymerase is better? - (reply: 2)
  378. polymerase to use for cloning - (reply: 4)
  379. Problem amplifying viral gene - (reply: 5)
  380. Adding tag using overlapping PCR - (reply: 2)
  381. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  382. Design primer from incomplete sequence... - (reply: 2)
  383. Design primer from incomplete sequence... - (reply: 2)
  384. How to perform colony PCR - (reply: 1)
  385. Primer designing for Methylation - (reply: 6)
  386. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  387. cDNA amplification problem - (reply: 4)
  388. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  389. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  390. PCR product running on agarose gel - (reply: 32)
  391. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  392. No bands in PCR after DNase treatment - (reply: 4)
  393. PCR amplification with high fidelity enzyme - (reply: 1)
  394. PCR HELP!!!! - (reply: 1)
  395. Overlap PCR problem - (reply: 5)
  396. PCR and restriction enzyme digestion - (reply: 3)
  397. Bisulfite PCR and cloning - (reply: 5)
  398. PCR product purification - (reply: 4)
  399. Overlapping sequence PCR primers - (reply: 1)
  400. restricted PCR plasmid runs slower - (reply: 2)
  401. BSP PCR primer design explained - (reply: 11)
  402. Bisulfite sequencing PCR worked - (reply: 5)
  403. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  404. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  405. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  406. doing PCR using PCR product , help plz !! - (reply: 5)
  407. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  408. good 16S univ. primer for qPCR? - (reply: 3)
  409. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  410. Amplification of CAG promoter problems - (reply: 4)
  411. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  412. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  413. I need help for designing a pair of primers for this sequence. - (reply: 1)
  414. PCR not working for 5 Aza treated DNA - (reply: 3)
  415. Differentiating mouse from human cells with PCR - (reply: 5)
  416. Differentiate between RT-PCR and PCR - (reply: 3)
  417. PCR band slightly BELOW expected length ?! - (reply: 14)
  418. long primers PCR - (reply: 4)
  419. unexpected band in PCR with plasmid - (reply: 4)
  420. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  421. DNA polymerase for GC rich template? - (reply: 5)
  422. Sequencing Primers and Plasmids - (reply: 3)
  423. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  424. DNA extraction - PCR Problem - (reply: 3)
  425. Primers to amplify Kan operon from pet28 - (reply: 2)
  426. PCR product biotinylation - (reply: 3)
  427. Tagged primers and a second PCR targetting the tags - (reply: 5)
  428. cloning pcr insert into plasmid - (reply: 2)
  429. Barcode generator for PCR primers - (reply: 2)
  430. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  431. Barcoding PCR products - (reply: 18)
  432. Biotinylated primers - (reply: 4)
  433. role of water in PCR - (reply: 3)
  434. HIV gene- PCR - (reply: 3)
  435. Real time PCR melting curve - (reply: 2)
  436. BSA in PCR reaction - (reply: 1)
  437. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  438. Cannot see insert with colony pcr - (reply: 6)
  439. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  440. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  441. How to Join two PCR products - (reply: 3)
  442. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  443. What's wrong with my PCR - (reply: 5)
  444. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  445. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  446. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  447. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  448. Cp, but no amplification - (reply: 2)
  449. pcr product digestion problem - (reply: 4)
  450. Low PCR product - (reply: 7)
  451. Control primers for bisulfite-converted DNA - (reply: 3)
  452. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  453. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  454. wrong dntp concentration - (reply: 2)
  455. Third round PCR - (reply: 2)
  456. long fragment PCR - (reply: 2)
  457. Best proof-reading polymerase? - (reply: 13)
  458. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  459. Q regarding primer mix for MSP!! - (reply: 4)
  460. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  461. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  462. I need to break this cycle of PCR issues - (reply: 13)
  463. Questions / Help with Fusion PCR - (reply: 8)
  464. Controls for RT-PCR reactions - (reply: 2)
  465. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  466. BSP Primers with M13 Tails - (reply: 1)
  467. Detection of T7 RNA polymerase by WB - (reply: 6)
  468. Multiplex PCR - (reply: 33)
  469. issues when designing PCR primers using PRIMER3 - (reply: 8)
  470. Primer catalog/databasing - (reply: 1)
  471. Nonrepetitive nested PCR - (reply: 2)
  472. Primer Blast - (reply: 6)
  473. Help designing primers for use in In-fushion cloning - (reply: 4)
  474. PCR internal control - (reply: 2)
  475. Primers Freeze-Thaw - (reply: 16)
  476. TDNA Genotyping PCR program - (reply: 1)
  477. primer design using 16s rRNA - (reply: 1)
  478. question about PCR and cloning - (reply: 1)
  479. PCR - (reply: 2)
  480. nonspecific band at 50bp in PCR - (reply: 9)
  481. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  482. PCR master mix - (reply: 7)
  483. Maximum PCR product Tm - (reply: 6)
  484. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  485. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  486. PCR & Southern blotting - (reply: 1)
  487. Bisulfite PCR Question - (reply: 2)
  488. Primer Design HELP! - (reply: 3)
  489. Help with sequencing of a 120bp PCR product. - (reply: 9)
  490. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  491. How are Primers made? - (reply: 9)
  492. Long Non-Specific PCR Products - (reply: 5)
  493. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  494. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  495. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  496. PCR calculation - (reply: 1)
  497. Colony PCR and digestion with KpnI - (reply: 10)
  498. designing methylation specific primers - (reply: 3)
  499. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  500. PCR - consistent false positive results - (reply: 6)
  501. PCR no amplification - (reply: 15)
  502. How to quantify virus by PCR - (reply: 12)
  503. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  504. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  505. Pcr product size determination for a fusion gene - (reply: 8)
  506. Quantify DNA before ChIP PCR - (reply: 3)
  507. Primer Annealing Temperatures - (reply: 24)
  508. my pcr product is larger than expected - (reply: 4)
  509. Question about PCR - DNA or RNA as template - (reply: 4)
  510. pcr purification - (reply: 1)
  511. pcr purification doubt - (reply: 4)
  512. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  513. PCR band moves lower than its size on agarose gel - (reply: 3)
  514. same primers, different product in conventional PCR and qPCR - (reply: 6)
  515. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  516. pcr problem - (reply: 6)
  517. NEB Q5 polymerase works very well - (reply: 1)
  518. Primers amplify genomic DNA but not cDNA - (reply: 2)
  519. pcr purification - (reply: 1)
  520. storing PCR product overnight - (reply: 2)
  521. Primer BLAST - (reply: 1)
  522. Bands in negative control PCR - (reply: 2)
  523. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  524. problem with pcr mutagenesis - (reply: 10)
  525. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  526. Use PCR purification kit to purify restriction digestion - (reply: 2)
  527. PCR primer design - published primers trustable? - (reply: 8)
  528. Polymerase for E. coli screenings - (reply: 5)
  529. complicated RT-PCR issues - (reply: 4)
  530. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  531. Can we use degenerate bases in BSP primers? - (reply: 1)
  532. Quick change mutagenesis No PCR product visible - (reply: 2)
  533. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  534. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  535. Can anyone help with my Bisulfite PCR? - (reply: 2)
  536. How to check primer sequences using BLAST and other tools? - (reply: 2)
  537. real time PCR analysis in patient samples - (reply: 2)
  538. carpet in gels for PCR products - (reply: 2)
  539. Primer Check? - (reply: 2)
  540. Sequencing of the PCR production in BSP - (reply: 1)
  541. amplification of 14Kb - (reply: 1)
  542. Validation of PCR primers/ probes - (reply: 6)
  543. Taq polymerase for MSP - (reply: 1)
  544. Real time PCR sudenly not working - (reply: 1)
  545. Buffer-composition One-Step RT-PCR - (reply: 4)
  546. Two reverse primer sequences for a single forward primer - (reply: 1)
  547. PCR with more than one primer - (reply: 1)
  548. RAPD - PCR problem - (reply: 5)
  549. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  550. How to best store PCR product? - (reply: 2)
  551. RT-PCR primer design - (reply: 1)
  552. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  553. Odd gel run following PCR - (reply: 28)
  554. Qiagen PCR Array Reagents? - (reply: 1)
  555. No or very weak band using 16s primers - (reply: 1)
  556. No PCR amplification with b-actin primers - (reply: 1)
  557. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  558. Site-directed, PCR works but no colonies - (reply: 2)
  559. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  560. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  561. No PCR amplified with long primers - (reply: 10)
  562. PCR optimization: PCR vs qPCR - (reply: 4)
  563. should I do blunt end my pcr product before ligation - (reply: 2)
  564. PCR smear for genomic samples - (reply: 4)
  565. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  566. another primer dilution question - (reply: 2)
  567. i need help with virus pcr - (reply: 2)
  568. Primer Efficiency - (reply: 2)
  569. Experimental set up for qRT PCR - (reply: 13)
  570. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  571. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  572. How should I optimize my PCR - (reply: 2)
  573. Second round of PCR after gel extraction fails miserably - (reply: 3)
  574. an effective way to do a yeast colony pcr - (reply: 2)
  575. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  576. urgent help plz with RT-PCR - (reply: 3)
  577. oligo(dt) 15 vs random primers - (reply: 3)
  578. Help to optain a long PCR produc - (reply: 3)
  579. Multiple bands in eluted PCR product - (reply: 1)
  580. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  581. Problems with two step RT-PCR - (reply: 2)
  582. Getting genomic DNA for PCR - (reply: 4)
  583. primer dilutions - (reply: 4)
  584. Genotyping PCR primers - (reply: 1)
  585. Conventional PCR - (reply: 1)
  586. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  587. PCR product loss on cleanup - (reply: 2)
  588. MSP Primers not working - (reply: 6)
  589. Primers too dilute - (reply: 1)
  590. Amplification curves jagged - (reply: 1)
  591. Cloning HELP- Possible Primer issues - (reply: 6)
  592. No PCR product in site-directed mutagenesis - (reply: 4)
  593. Giardia/Crypto real time PCR late amplification - (reply: 1)
  594. How to dilute the template DNA for PCR - (reply: 7)
  595. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  596. Need help for PCR - (reply: 5)
  597. PCR primer pairs - university exam - (reply: 7)
  598. unespecific band PCR from mouse genomic DNA - (reply: 9)
  599. Help with primer design through PRIMER3 - (reply: 4)
  600. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  601. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  602. Plasmid vs cDNA amplification by PCR - (reply: 2)
  603. Tm of primers - prediction and verification - (reply: 1)
  604. PCR cloning...help! - (reply: 5)
  605. RT-PCR contamination in negative control. - (reply: 1)
  606. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  607. how to design primers for 16sr RNA - (reply: 24)
  608. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  609. Help with primer design - (reply: 3)
  610. Primer Tm calculation. - (reply: 1)
  611. low concentration of purified PCR product - (reply: 2)
  612. PCR smearing and...problems! - (reply: 7)
  613. Running Primers on Agarose Gel - (reply: 6)
  614. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  615. universal primers for PCR ribotyping - (reply: 6)
  616. will the template of PCR be added A ? - (reply: 2)
  617. Colony PCR not working - (reply: 4)
  618. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  619. Primer design: Tm above or below Ta? - (reply: 5)
  620. Oligo primers for shRNA construction - (reply: 1)
  621. Design primers for expression cloning - (reply: 11)
  622. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  623. primer dilution query - (reply: 2)
  624. Real Time PCR polymerase enzyme - (reply: 3)
  625. Estimating size of PCR products in a agarose gel - (reply: 4)
  626. Sequencing of PCR product - (reply: 4)
  627. PCR contamination problem - (reply: 2)
  628. Using BLAST to check primers - (reply: 1)
  629. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  630. What is the function of a third primer in a PCR mix?? - (reply: 2)
  631. How can we design a primer ? step by step method - (reply: 4)
  632. cDNA synthesis or RT-PCR - (reply: 3)
  633. PCR on cDNA and SGamplification??? - (reply: 1)
  634. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  635. real time PCR protocol - controls? - (reply: 2)
  636. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  637. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  638. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  639. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  640. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  641. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  642. PCR and UV spectrophotometry - (reply: 3)
  643. RT-PCR in a single tube - (reply: 3)
  644. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  645. annealing temperature for 3 primer pairs? - (reply: 4)
  646. PCR Reaction - (reply: 3)
  647. Impact of primer sequence on TA cloning - (reply: 3)
  648. problems with Bisulfite PCR - (reply: 1)
  649. low pcr product - (reply: 3)
  650. overlapping PCR protocol - (reply: 12)
  651. PCR to amplify my insert not working - (reply: 6)
  652. Problem with PCR amplification- high 3' stability - (reply: 3)
  653. Home made real-time PCR problem - (reply: 4)
  654. High RNase concentration interfering with PCR? - (reply: 8)
  655. Pcr primers - (reply: 2)
  656. PCR failure from yeast genomic DNA - (reply: 1)
  657. failure of PCR from yeast genomic DNA - (reply: 1)
  658. RT-Q PCR primer design question - (reply: 2)
  659. high MW dimers - (reply: 2)
  660. Strange RT-PCR amplification plots - (reply: 9)
  661. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  662. What products will I get from this pcr? - (reply: 1)
  663. PCR buffer without Tris - (reply: 1)
  664. PCR Water - (reply: 4)
  665. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  666. Which Brand of real-time PCR machine - (reply: 6)
  667. Problem with PCR - large extra bands - (reply: 3)
  668. big differnece in my primers Tm - (reply: 2)
  669. Designing primers for the ORFs - (reply: 9)
  670. Two-Step PCR - (reply: 2)
  671. Gradient PCR - (reply: 2)
  672. PCR with phophorylated primers - (reply: 1)
  673. Problem with New qPCR primers - help! - (reply: 6)
  674. Designing primers for miRNA located on minus strand. - (reply: 2)
  675. Plasmid sequencing without primers - (reply: 1)
  676. Smallest insert in PCR - (reply: 6)
  677. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  678. loop-mediated amplification - (reply: 2)
  679. Blunt end PCR product ligation - (reply: 1)
  680. Help with high Ct values for Real-Time PCR - (reply: 1)
  681. Genotyping by allele specific PCR - (reply: 6)
  682. RT-PCR primers and the poly(A) tail - (reply: 2)
  683. NO Insert found in the Colony PCR - (reply: 15)
  684. No band in PCR - (reply: 5)
  685. overlapping primer - (reply: 1)
  686. PCR primers help - (reply: 5)
  687. PCR smear after one-month storage - (reply: 2)
  688. Primer dimers - (reply: 2)
  689. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  690. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  691. Simple question of dNTP's for PCR - (reply: 5)
  692. Need help for PCR for AT rich gene - (reply: 3)
  693. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  694. Primer validation and PCR efficiency - (reply: 4)
  695. How to calculate the price of PCR test per sample? - (reply: 6)
  696. Contamination in negative control of PCR (No template control) - (reply: 6)
  697. cloning with nested pcr - (reply: 3)
  698. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  699. PCR is amplifying non-specific fragment - (reply: 6)
  700. ChIP amplification problems - (reply: 1)
  701. real time RT-PCR problems - (reply: 2)
  702. should I design primers spanning both intron and exon - (reply: 2)
  703. RT-PCR - (reply: 1)
  704. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  705. Template DNA (plasmid) concentration for PCR - (reply: 4)
  706. PCR with a very long and a short primer - (reply: 5)
  707. Ligation of 16kb PCR product - (reply: 5)
  708. Multiplex PCR does not work - (reply: 3)
  709. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  710. Problem with genomic PCR (2.4 kb) - (reply: 1)
  711. Comparison between two primers pairs? Is it possible? - (reply: 3)
  712. Rt-PCR primer design - (reply: 7)
  713. Length of non-binding sequence in primers with RE site - (reply: 6)
  714. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  715. Getting a smaller PCR product than required - (reply: 6)
  716. Difficult sequences in PCR - (reply: 2)
  717. why is real time pcr curve like this? - (reply: 3)
  718. PCR inconsistency - (reply: 2)
  719. Orientation of primers - (reply: 2)
  720. PCR, neg control & no. of cycles - (reply: 2)
  721. Strange PCR result from genomic DNA - (reply: 3)
  722. Bright bands of PCR products stick in gel wells - (reply: 1)
  723. methylation analysis with MS-HRM (primer design) - (reply: 1)
  724. whole plasmid pcr? - (reply: 6)
  725. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  726. salts in PCR - (reply: 4)
  727. Strange amplification plot - (reply: 4)
  728. Conventional PCR of different dilutions - (reply: 1)
  729. primer resuspension - (reply: 2)
  730. Principles of overlap PCR - (reply: 7)
  731. ligation of pcr product into expression vector - (reply: 4)
  732. Unable to PCR!!!! - (reply: 3)
  733. Help designing primers with restriction sites - (reply: 3)
  734. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  735. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  736. Abnormal amplification curve - (reply: 1)
  737. Problem with Semiquantitative PCR - (reply: 4)
  738. Advice on optimising bisulfite PCR sought - (reply: 2)
  739. Help: I am loosing the DNA during PCR purification - (reply: 7)
  740. PCR primer with no extra DNA end - (reply: 5)
  741. PCR colonies of Pichia pastoris - (reply: 1)
  742. ROX reference dye for quantitative RT-PCR - (reply: 1)
  743. PCR problem - (reply: 9)
  744. why PCR product smeared? - (reply: 2)
  745. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  746. silincing box, no amplification - (reply: 3)
  747. Comparative semiquantitative RT-PCR - (reply: 2)
  748. Bands in my PCR Controls - (reply: 3)
  749. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  750. Strange band in colony PCR - (reply: 2)
  751. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  752. Multiplex PCR - (reply: 12)
  753. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  754. PYO pcr stopped working. - (reply: 1)
  755. Bands seen with qPCR missing in regular PCR - (reply: 5)
  756. checking msp primers - (reply: 2)
  757. PCR product one day, none the next day - (reply: 4)
  758. PCR on colony - (reply: 10)
  759. PCR reaction ISSUES???? need help - (reply: 4)
  760. PCR using CDNA as template - (reply: 2)
  761. RT-PCR doesn't work with all RNA used - (reply: 1)
  762. Need help amplifying repeating sequence. - (reply: 5)
  763. Real Time PCR 101...help! - (reply: 1)
  764. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  765. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  766. Another primer dimers problem - (reply: 23)
  767. Colony PCR Problem!!!! - (reply: 5)
  768. using PCR product as standard template - (reply: 3)
  769. PCR kit - (reply: 7)
  770. Make additional cycles with an already finished pcr product - (reply: 3)
  771. Amplification in water control but not in samples - (reply: 3)
  772. PCR products form with DNA, but not with c-DNA - (reply: 2)
  773. question about pcr amplification efficiency? - (reply: 1)
  774. DNA polymerase: recombinant or native? - (reply: 3)
  775. pcr clean-up fail? - (reply: 1)
  776. MSP U primers very difficult to amplify... - (reply: 4)
  777. No gene amplification, 18s amplification is fine - (reply: 1)
  778. ChIP-qPCR gives odd amplification plot - (reply: 5)
  779. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  780. sequential cloning of multiple PCR products - how to do? (reply: 3)
  781. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  782. Primer design - (reply: 3)
  783. Tomato Actin PCR+Images included - (reply: 2)
  784. single primer PCR - (reply: 5)
  785. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  786. cDNA synthesis + amplification - (reply: 2)
  787. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  788. LacZ PCR Problems - (reply: 1)
  789. cells stably expressing T7 RNA polymerase - (reply: 1)
  790. Primer concentration - (reply: 4)
  791. nested PCR with low target DNA? - (reply: 2)
  792. RT-PCR calibrator - (reply: 2)
  793. RNA - extraction and pcr (reply: 1)
  794. problem in pcr - (reply: 2)
  795. Can someone recommend me a virtual PCR software? - (reply: 6)
  796. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  797. Primer 3' mismatch - Strange experience (reply: 9)
  798. real-time PCR after restriction enzyme digestion - (reply: 6)
  799. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  800. Adding loading/tracking dye to PCR mix? - (reply: 10)
  801. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  802. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  803. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  804. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  805. how to get rid of non specific bands in PCR - (reply: 1)
  806. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  807. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  808. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  809. PCR cloning problem - could not get colony (reply: 4)
  810. pcr contamination - (reply: 4)
  811. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  812. Large Non-specfic bands in PCR - (reply: 3)
  813. PCR clonning - (reply: 3)
  814. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  815. 2 bands in blue colony PCR - (reply: 5)
  816. Primer dilution --> problem.. - (reply: 16)
  817. Help! Smear in Real-Time PCR Product - (reply: 1)
  818. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  819. Sybr green RT-qPCR primer - (reply: 1)
  820. Sybr green RT-qPCR primer - (reply: 1)
  821. primer design - microalgae (reply: 2)
  822. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  823. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  824. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  825. PCR yield in ng/ul - (reply: 1)
  826. No Amplification in PCR - (reply: 4)
  827. Real time PCR and percentage loss - (reply: 1)
  828. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  829. Problem with smear in PCR with certain templates - (reply: 4)
  830. Real time PCR in the presence of heme - (reply: 3)
  831. issues in PCR amplification - (reply: 2)
  832. Problems with PCR in general - (reply: 1)
  833. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  834. how to overcome primer contamination - need protocol (reply: 11)
  835. RT-PCR primer design - Primer design and evaluation (reply: 7)
  836. CHIP analysis by PCR - (reply: 4)
  837. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  838. Primer,probes dilution - (reply: 3)
  839. Splice varient quantification in RT real time PCR? - (reply: 1)
  840. negative control for msp pcr - (reply: 2)
  841. Calculating geometric mean for real-time PCR - (reply: 5)
  842. template transfer? - how does the rna polymerase switches template? (reply: 1)
  843. PCR Gel nonspecific band - (reply: 13)
  844. semiquantitative pcr - (reply: 1)
  845. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  846. PCR: Cloning Primers - (reply: 8)
  847. PCR product - (reply: 5)
  848. pcr amplify an insert in plasmid? - (reply: 2)
  849. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  850. Exon Spanning PCR Primers - (reply: 1)
  851. Colony PCR - (reply: 1)
  852. PCR to confirm double crossover - (reply: 1)
  853. Primer sequences - (reply: 1)
  854. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  855. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  856. PCR Primers - (reply: 2)
  857. Cleaning up RT-PCR product before sequencing - (reply: 1)
  858. Template Amount for PCR Amplification - (reply: 6)
  859. How to blast methylation specific and unspecific primers - (reply: 1)
  860. RT-PCR cDNA synthesis - (reply: 6)
  861. problems amplifying from cDNA - (reply: 4)
  862. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  863. No DNA after PCR purification with QiaQuick? - (reply: 4)
  864. restriction digestion against colony PCR - (reply: 1)
  865. How to check primers are of correct sequence or not? - (reply: 2)
  866. Primers... - (reply: 1)
  867. When are primer dimers a problem? - (reply: 1)
  868. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  869. PCR: always got band in the negative control - (reply: 8)
  870. primer with higher melting temperature - (reply: 2)
  871. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  872. Problems designing primers for BSP - (reply: 2)
  873. qPCR primer design - possible off-target (reply: 1)
  874. Primer dimer formation and real-time PCR - (reply: 7)
  875. Identify PCR products - (reply: 3)
  876. PCR genomic DNA using cell lysate - (reply: 1)
  877. Maximum size of overhangs in PCR - (reply: 6)
  878. Colony PCR doesn't work anymore - (reply: 5)
  879. RT-PCR t-test, and ANOVA - (reply: 1)
  880. Primer Design Urgent! - (reply: 1)
  881. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  882. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  883. PCR of microRNA first strand cDNA - (reply: 1)
  884. how to know my primer's sense ??? - help ! (reply: 2)
  885. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  886. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  887. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  888. intron spanning primers for non model organisms - (reply: 2)
  889. Primer design - (reply: 2)
  890. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  891. qPCR amplification interference fixed with freezing - (reply: 6)
  892. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  893. software for quantifing PCR product band - (reply: 1)
  894. Multiple bands from purified PCR product - (reply: 3)
  895. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  896. Doing PCR on Nebulized DNA - (reply: 2)
  897. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  898. RT-PCR - (reply: 9)
  899. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  900. inverse pcr - (reply: 2)
  901. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  902. Primer seq - (reply: 2)
  903. Urgent: should you dry PCR product - (reply: 13)
  904. PCR product size - (reply: 6)
  905. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  906. oligo dt and random primer - (reply: 3)
  907. mRNA Search for RT-PCR (U to T) - (reply: 2)
  908. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  909. PCR Mastermix - (reply: 4)
  910. PCR triplicates versus one reaction - differences? (reply: 17)
  911. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  912. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  913. Primers and self and hetero dimers - (reply: 1)
  914. Really weird amplification curves - (reply: 1)
  915. Primers for qPCR - (reply: 3)
  916. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  917. RT-PCR primer - (reply: 3)
  918. PCR problem - basic PCR for plasmid amplification (reply: 2)
  919. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  920. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  921. Unpredictablity of PCR product - (reply: 5)
  922. total cDNA amplification by PCR - (reply: 2)
  923. Detection limit of a conventional PCR - (reply: 2)
  924. PCR for MULTIPLE mutagenesis - (reply: 4)
  925. Using Sybr green in realtime pcr - (reply: 1)
  926. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  927. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  928. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  929. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  930. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  931. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  932. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  933. Digested PCR product migrate slower than uncut - (reply: 8)
  934. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  935. PCR question - (reply: 2)
  936. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  937. 2 rounds PCR got problem - (reply: 2)
  938. PCR help minus strand - Primer design (reply: 1)
  939. chloramphenicol storage and amplification - (reply: 3)
  940. Smear in long distance PCR - (reply: 39)
  941. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  942. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  943. TOUCH DOWN PCR - (reply: 2)
  944. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  945. PCR stopped working - After changing buffer (reply: 5)
  946. ChIP PCR question - (reply: 33)
  947. PCR protocol questions! - (reply: 2)
  948. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  949. DNA amount calculation for PCR - (reply: 14)
  950. ChIP primer design - (reply: 1)
  951. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  952. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  953. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  954. How to analyze ChIP PCR data - (reply: 10)
  955. Questions regarding RT-PCR optimization - (reply: 2)
  956. number of copies after pcr? - (reply: 1)
  957. PCR double-bands - (reply: 1)
  958. Problems with semi-quatitative PCR - (reply: 1)
  959. Question about Primers - (reply: 3)
  960. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  961. Large fragment amplification failed - (reply: 3)
  962. purification of PCR product for cloning in a vector - (reply: 3)
  963. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  964. PCR reaction calculation - (reply: 2)
  965. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  966. Primer design with a tag - (reply: 4)
  967. no increase in fluorescence in my real time PCR - (reply: 3)
  968. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  969. Help with real-time PCR quantification of miRNA - (reply: 8)
  970. PCR, using gel extracted band as a template - (reply: 4)
  971. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  972. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  973. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  974. Combining forward and reverse primer gives different size on gel - (reply: 2)
  975. Q-PCR and RT-PCR - (reply: 3)
  976. calculating total amount - in a pcr reaction (reply: 4)
  977. Problems with amplifying microRNA - (reply: 3)
  978. PCR of complement genomic DNA - (reply: 3)
  979. primers for fungal identification - (reply: 9)
  980. confusing PCR - (reply: 1)
  981. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  982. PCR for long and repetitive region from genomic DNA - (reply: 4)
  983. difference between PCR primers and sequencing primers - (reply: 1)
  984. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  985. PCR of repeated region - (reply: 1)
  986. Sense and antisense DNA, and primer design - (reply: 6)
  987. PCR bands in NTC but NOT in negative samples - (reply: 2)
  988. housekeeping genes in PCR - (reply: 1)
  989. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  990. Question about RealTime PCR Primer Design - (reply: 1)
  991. Designing a Primer with a RS that has a W - (reply: 1)
  992. Primers stopped working!? - PCR Primers (reply: 6)
  993. NFK beta primers - (reply: 4)
  994. final PCR product - need clarifications (reply: 1)
  995. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  996. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  997. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  998. RT-PCR result !! - (reply: 4)
  999. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  1000. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  1001. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  1002. why genomic can't be use for pcr? - (reply: 2)
  1003. Primer design - Primer design (reply: 2)
  1004. Re-use unamplified PCR product - (reply: 2)
  1005. Reason for odd PCR conditions - (reply: 3)
  1006. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  1007. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  1008. pcr - problem in pcr (reply: 2)
  1009. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  1010. How to present and analyze these real-time PCR data? - (reply: 2)
  1011. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  1012. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  1013. PCR Master mix - (reply: 2)
  1014. cDNA storage and real time RT PCR - (reply: 3)
  1015. Real Time PCR Primers and Probes - (reply: 2)
  1016. Anyone make their own PCR cloning vector? - (reply: 5)
  1017. Stability of PCR T-overhangs - (reply: 1)
  1018. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  1019. PCR product wrong size! Need help! - (reply: 9)
  1020. pooling different pcr cycles? - (reply: 1)
  1021. Amplification of concatenated linear ligated fragments - (reply: 4)
  1022. Clinical Real-time PCR assay - (reply: 1)
  1023. Need help - How we can design primer for miRNA (reply: 1)
  1024. primer and promoter sequences - (reply: 1)
  1025. Standard curve for real-time PCR - (reply: 2)
  1026. designing of primers - (reply: 2)
  1027. Primer Tm differences/ PCR - (reply: 1)
  1028. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  1029. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  1030. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  1031. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  1032. Designing primer to remove his-tag - (reply: 1)
  1033. PCR Primer Dilution from F+R - (reply: 1)
  1034. primer reconstitution - primer reconstitution (reply: 4)
  1035. Not-Quite-Nested PCR - (reply: 2)
  1036. PCR product too short - (reply: 6)
  1037. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  1038. Primer design for insert amplification - Is this correct? (reply: 2)
  1039. got smear on the PCR gel !!! - (reply: 4)
  1040. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  1041. "Unzipped" PCR band - (reply: 3)
  1042. Review on new amplification techniques - (reply: 2)
  1043. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  1044. DOT BLOT for pcr product - No result obtain (reply: 1)
  1045. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  1046. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  1047. HRM primers - (reply: 1)
  1048. PCR amplification with Pfu / quality of DNA - (reply: 4)
  1049. primer design - (reply: 2)
  1050. Is it possible to compare data between real-time PCR plates - (reply: 1)
  1051. Conventional PCR problems - wisamni2004 (reply: 2)
  1052. New to sequencing, primer design - (reply: 2)
  1053. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  1054. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  1055. incorrect/smaller band size for real time PCR - (reply: 1)
  1056. Quickchange mutagenesis primer - (reply: 3)
  1057. pcr / primer theory in bisulfite sequencing - (reply: 4)
  1058. homologous amplicon for real time pcr - (reply: 1)
  1059. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  1060. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  1061. assistance with PCR amplification please - (reply: 3)
  1062. I need an urgent help with q-pcr amplification plots - (reply: 11)
  1063. Problems with the Specificity of the Primers - (reply: 2)
  1064. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  1065. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  1066. Degenerate primers PCR problem, Please help! - (reply: 2)
  1067. Hotstart PCR and unspecific Amplification - (reply: 3)
  1068. Primer design help needed - (reply: 1)
  1069. Primers no longer work - (reply: 1)
  1070. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  1071. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  1072. RT PCR for known snps detection? - SNPs detection (reply: 1)
  1073. colony PCR - (reply: 2)
  1074. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  1075. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  1076. PCR reaction without template gives a product - (reply: 6)
  1077. PCR wrong product size - (reply: 6)
  1078. Wrong pcr product size - (reply: 4)
  1079. real time PCR machine - (reply: 5)
  1080. PCR very faint product - (reply: 1)
  1081. Question about principle of PCR - (reply: 3)
  1082. PCR amplification before bisulfite conversion - (reply: 6)
  1083. 4 question for real time PCR - (reply: 5)
  1084. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  1085. PCR didn't work - suspect the enzyme (reply: 2)
  1086. Find universal primers in vector - any online tool? (reply: 6)
  1087. Picking primers to confirm Illumina meth27 results - (reply: 2)
  1088. Colony PCR Specificity - (reply: 2)
  1089. Colony Pcr - primers - (reply: 5)
  1090. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  1091. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  1092. Primer Dilution Problem - D'oh! (reply: 2)
  1093. Using PCR to create overhangs - (reply: 3)
  1094. Creating overhangs with PCR - (reply: 4)
  1095. Problem with PCR on bacmids - (reply: 1)
  1096. Is there any software that can be sued to design degenerate primers - (reply: 1)
  1097. Running real time pcr product on gel?.. - (reply: 4)
  1098. Primer Decontamination - (reply: 2)
  1099. PCR mutagenesis of plasmid - (reply: 5)
  1100. tools for checking non-specific binding of primers -
    (reply: 1)
  1101. Primer designed for ARMS PCR - (reply: 2)
  1102. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  1103. methylation specific PCR - Primers - (reply: 1)
  1104. bacterial identification using real time PCR - (reply: 1)
  1105. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1106. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1107. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1108. colony PCR for subcloning gene - (reply: 1)
  1109. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1110. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1111. PCR reamplification - (reply: 2)
  1112. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1113. How many colonies to screen (colony PCR)? - (reply: 4)
  1114. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1115. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1116. question about pcr - (reply: 3)
  1117. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1118. How to construct a standard curve for real time PCR - (reply: 1)
  1119. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1120. PCR with one primer - (reply: 1)
  1121. colony PCR inconsistent - (reply: 4)
  1122. PCR contamination - (reply: 19)
  1123. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1124. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1125. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1126. PCR not working - No amplification - (reply: 5)
  1127. Quantification of PCR products - (reply: 1)
  1128. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1129. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1130. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1131. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1132. Nested PCR question - (reply: 1)
  1133. High Tm on primers - cannot get a product - (reply: 8)
  1134. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1135. undesired products in multiplex PCR - (reply: 3)
  1136. NCBI primer blast problem - (reply: 1)
  1137. Help with Multiplex Nested PCR - (reply: 2)
  1138. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1139. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1140. primer sequence problem - (reply: 2)
  1141. Finding bisulfite PCR primer sequence - (reply: 3)
  1142. PCR -No band formation - (reply: 1)
  1143. PCR - No Band formation - (reply: 3)
  1144. No amplification after bisulfite treatment - (reply: 4)
  1145. PCR AMPLIFICATION - (reply: 1)
  1146. Ligation of Blunt PCR Product - (reply: 1)
  1147. real time pcr need advise - (reply: 2)
  1148. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1149. PCR & Cloning - (reply: 6)
  1150. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1151. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1152. DNA pooling for PCR - Saving money PCR (reply: 7)
  1153. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1154. PCR need some help - (reply: 4)
  1155. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1156. Left polymerase out overnight - Will it still work? (reply: 1)
  1157. Primer Reconstitution--does temperature matter? - (reply: 5)
  1158. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1159. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1160. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1161. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1162. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1163. no PCR product from input... - (reply: 8)
  1164. MSP - unwanted amplification - (reply: 5)
  1165. Smears in Bisulfite seq. PCR - (reply: 3)
  1166. Primer design for qPCR - (reply: 2)
  1167. Primer design for qPCR - (reply: 1)
  1168. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1169. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1170. Multiple bands after bisulfite PCR - (reply: 7)
  1171. PCR genomic DNA of high GC content - (reply: 6)
  1172. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1173. primer design tips - (reply: 3)
  1174. PCR with Biotin Incorporation - (reply: 2)
  1175. pcr product - hello all (reply: 1)
  1176. design pcr primer - (reply: 1)
  1177. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1178. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1179. PCR with long and complex primers - (reply: 2)
  1180. Phospholylation of primers - (reply: 1)
  1181. apoptosis by PCR? - (reply: 12)
  1182. Re-using PCR plates? - (reply: 2)
  1183. Touchdown PCR issues - (reply: 3)
  1184. LINE1 OFR2 Primer Design - (reply: 1)
  1185. phosphorylation of primers using PNK - (reply: 1)
  1186. RT-PCR contamination issue - (reply: 3)
  1187. Primers for yeast (18S) - (reply: 1)
  1188. How to reduce the bisulfite PCR bias? - (reply: 4)
  1189. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1190. Primer Concentration to lower Ct - (reply: 1)
  1191. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1192. Primer Concentration Help - (reply: 4)
  1193. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1194. Primer Degradation - What exactly happens? (reply: 1)
  1195. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1196. PCR/DNA Extraction Problem - (reply: 5)
  1197. Primers in 2 exons - (reply: 2)
  1198. NTC appear in real time pcr - (reply: 6)
  1199. Problems with designing a primer - (reply: 2)
  1200. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1201. PCR of AT-rich DNA - (reply: 1)
  1202. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1203. Overlapping PCR - really need help ! - (reply: 5)
  1204. Freezing PCR product??? - (reply: 1)
  1205. problem with pcr cloning from mouse cDNA - (reply: 2)
  1206. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1207. PCR with Platinum Taq - product yield issues - (reply: 3)
  1208. real time PCR - (reply: 7)
  1209. Different sized product for same BS primer! - (reply: 2)
  1210. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1211. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1212. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1213. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1214. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1215. primers with restriction site - (reply: 1)
  1216. PCR product appear two close band in my gel - (reply: 15)
  1217. The Case of a Missing Band: PCR Issue - (reply: 4)
  1218. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1219. cDNA as PCR template - (reply: 1)
  1220. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1221. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1222. paranormal qpcr amplification activity - melting curves (reply: 3)
  1223. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1224. PCR template concentration - (reply: 6)
  1225. Bands in PCR negative control - (reply: 3)
  1226. PCR efficiency calculated by Linreg - (reply: 3)
  1227. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1228. primer design by generunner DG - (reply: 1)
  1229. degenerate pcr - (reply: 1)
  1230. BSP primers design, help please - (reply: 2)
  1231. primers with fluoresent dye PCR cycle question - (reply: 4)
  1232. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1233. Smears on PCR products - (reply: 4)
  1234. Dumb Reverse Transcription PCR Question - (reply: 2)
  1235. Reverse transcription PCR - (reply: 1)
  1236. PCR/RT-PCR beads - (reply: 2)
  1237. High Amplification Efficiency in Std. Curve - (reply: 5)
  1238. Non specific products in Bisulphite pcr - (reply: 4)
  1239. Run PCR amplifications in agarose gels - (reply: 3)
  1240. Problems with validating primers and low expression genes - (reply: 3)
  1241. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1242. Bisulfite sequencing PCR not working - (reply: 5)
  1243. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1244. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1245. intron/exon spanning primers - (reply: 1)
  1246. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1247. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1248. PCR product for sequencing - sample storage?? (reply: 2)
  1249. Absolute quantification using Real Time PCR - (reply: 2)
  1250. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1251. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1252. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1253. MSP primer troubles - (reply: 3)
  1254. Measuring global methylation using real time PCR - (reply: 2)
  1255. Designing Primers for multiple Isoforms - (reply: 1)
  1256. RT-PCR standard curve dilutions - (reply: 2)
  1257. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1258. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1259. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1260. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1261. PCR product dimer issue - (reply: 24)
  1262. PCR additives Formamide - Formamide which one? (reply: 4)
  1263. PCR Master mix - (reply: 1)
  1264. Addition of Restriction sites into PCR primers - (reply: 4)
  1265. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1266. failure PCR amplification from low GC content gene - (reply: 5)
  1267. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1268. conventional v real-time PCR applications - (reply: 1)
  1269. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1270. PCR reagents, can I use reagents from different manufacturers? - (reply: 6)
  1271. different PCR primers for real-time and classic PCR - (reply: 3)
  1272. primers deconamination??? - (reply: 6)
  1273. PCR band too thick - PCR troubleshooting question (reply: 5)
  1274. RT-PCR problem - (reply: 1)
  1275. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1276. primer check!!! - (reply: 6)
  1277. PCR bands on gel electrophoresis - (reply: 5)
  1278. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1279. long range PCR - (reply: 2)
  1280. Annealing Temperature of biotinylated primers - (reply: 2)
  1281. RT-PCR - New to RT-PCR info (reply: 2)
  1282. PCR punch - (reply: 4)
  1283. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1284. PCR off plasmid for screening - (reply: 2)
  1285. PCR failed no product.. help - (reply: 10)
  1286. number of molecules in PCR - (reply: 3)
  1287. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1288. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1289. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1290. Primer dilution - PCR primer dilution (reply: 2)
  1291. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1292. Can't re-PCR the PCR product - (reply: 5)
  1293. PCR reagent autoclave - (reply: 2)
  1294. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1295. Long Primers for PCR - (reply: 2)
  1296. Data Analysis for Real-time PCR - (reply: 2)
  1297. PCR not working - overhangs too long? (reply: 3)
  1298. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1299. argh more pcr headaches! - (reply: 6)
  1300. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1301. Chlamydia PCR cycling conditions - (reply: 1)
  1302. Primers going "loopy" - (reply: 2)
  1303. Conversion of primer unit - ug/ul to uM (reply: 4)
  1304. mutagenesis by PCR or just use a kit - (reply: 5)
  1305. Unsuccessful BS PCR - (reply: 3)
  1306. Primer Trouble Shooting - (reply: 5)
  1307. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1308. Smearing on gel in PCR products - (reply: 5)
  1309. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1310. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1311. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1312. Relative RT-PCR - (reply: 2)
  1313. Relative real time RT PCR - (reply: 2)
  1314. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1315. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1316. a simple primer problem - (reply: 5)
  1317. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1318. no or poor amplification - (reply: 1)
  1319. Primers from 3'UTR - (reply: 2)
  1320. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1321. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1322. Designing primers with ESTs - (reply: 2)
  1323. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1324. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1325. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1326. Real Time vs Traditional PCR results - (reply: 1)
  1327. The problems to identify mouse genotype with PCR - (reply: 1)
  1328. addition of BclI restriction site to PCR primer - (reply: 6)
  1329. non specific amplification - DNA amplification by PCR (reply: 1)
  1330. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1331. How does methprimer calculate primer Tm - (reply: 2)
  1332. Laminar Flow vs PCR cabinet - (reply: 4)
  1333. How to do PCR to detect mRNA without RT? - (reply: 2)
  1334. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1335. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1336. PCR has stopped working - (reply: 7)
  1337. PCR negative control contamination - (reply: 8)
  1338. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1339. Phosphorylated primers - (reply: 5)
  1340. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1341. PCR efficiency in real timePCR - (reply: 5)
  1342. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1343. Drastic Decrease in PCR product yield - (reply: 2)
  1344. resources on PCR principles & technique - (reply: 1)
  1345. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1346. Colony Screeing without using PCR - (reply: 8)
  1347. High MW PCR band seen...help needed - (reply: 7)
  1348. QPCR - Primer and Probe question - QPCR (reply: 2)
  1349. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1350. standard curve/ PCR efficiency problems... - (reply: 1)
  1351. Help! PCR that used to work doesn't work now! - (reply: 2)
  1352. PCR and templates - (reply: 3)
  1353. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1354. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1355. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1356. Easy primer question? - Primers (reply: 1)
  1357. PCR inconsistency - (reply: 4)
  1358. Plasmid problem - From PCR product (reply: 7)
  1359. help needed: PCR a gene from genomic DNA - (reply: 2)
  1360. questions about pcr products after pooling - (reply: 3)
  1361. stiching/linking/sewing pcr - (reply: 3)
  1362. Human mtDNA amplification problems - (reply: 3)
  1363. primer dimer - proplems (reply: 1)
  1364. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1365. Nested PCR - (reply: 2)
  1366. pfu vs long pcr mix - (reply: 7)
  1367. weird PCR ask for help - (reply: 12)
  1368. Methylation specific DMSO PCR - (reply: 1)
  1369. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1370. No PCR product at all - (reply: 8)
  1371. PCR troubleshoooooting - primer dimer (reply: 1)
  1372. Primer efficiency test - (reply: 1)
  1373. primer software - (reply: 2)
  1374. Question on wired PCR - (reply: 1)
  1375. restriction digestion of PCR product - (reply: 7)
  1376. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1377. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1378. How to know in which exon a primer match? - (reply: 1)
  1379. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1380. Amplification of human genomic DNA - (reply: 2)
  1381. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1382. sequencing with forward/reverse primers - (reply: 7)
  1383. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1384. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1385. Adding A overhangs - primer design implications? - (reply: 1)
  1386. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1387. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1388. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1389. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1390. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1391. primer design problem - (reply: 1)
  1392. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1393. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1394. Problem with primer efficiency analysis - (reply: 4)
  1395. slan real time PCR system for validation of microarray results? - (reply: 1)
  1396. PCR unusualband at ~230bps - PCR (reply: 1)
  1397. DMSO or BSA for PCR - (reply: 6)
  1398. Whole Genome Amplification - (reply: 2)
  1399. primer design - (reply: 1)
  1400. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1401. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1402. designing primers for genes not sequenced yet - (reply: 2)
  1403. Tris Buffer for PCR reaction - why? (reply: 1)
  1404. PCR a plasmid protein - (reply: 2)
  1405. multiplex PCR - (reply: 4)
  1406. Realtime PCR machine - (reply: 2)
  1407. PCR set-up calculation nightmares. - (reply: 3)
  1408. Primer desing - (reply: 2)
  1409. Colony PCR - (reply: 3)
  1410. Standard curves for PCR efficiency. - (reply: 2)
  1411. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1412. Question about RAPD PCR - (reply: 2)
  1413. Looking for help with my PCR! - (reply: 3)
  1414. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1415. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1416. primer design - (reply: 9)
  1417. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1418. PCR without thermal cycler? - (reply: 9)
  1419. primer contamination - primer contaminated with ice (reply: 3)
  1420. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1421. RT-PCR problem - (reply: 4)
  1422. Only DNA ladder , No desired band in PCR - (reply: 4)
  1423. taq and PCR - (reply: 6)
  1424. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1425. PCR Efficiency - (reply: 2)
  1426. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1427. Long PCR and genomic DNA isolation problems - (reply: 2)
  1428. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1429. NTC with specific amplification - (reply: 1)
  1430. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1431. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1432. Primer3 vs Primer BLAST - (reply: 3)
  1433. PCR and then ligation - (reply: 7)
  1434. Primer design: free energy - (reply: 2)
  1435. Amplification in NTC and noRT controls - (reply: 5)
  1436. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1437. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1438. asymmetric PCR - (reply: 1)
  1439. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1440. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1441. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1442. trouble with pcr - (reply: 8)
  1443. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1444. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1445. Real time PCR results-interpretation - (reply: 1)
  1446. Real time PCR results-interpretation - (reply: 2)
  1447. Primer optimization for ChIP - (reply: 2)
  1448. sequencing primer: T7 or T7promoter? - (reply: 4)
  1449. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1450. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1451. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1452. primer tm - tm calculation for tagged primers (reply: 2)
  1453. Reusing 96 well plates for PCR - (reply: 2)
  1454. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1455. PCR and AFLP - (reply: 1)
  1456. Universal 16s rRNA primers needed - (reply: 3)
  1457. Identifying PCR Inhibitors - (reply: 10)
  1458. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1459. PCR + phusion enzyme = massive errors - (reply: 4)
  1460. long DNA amplification - (reply: 11)
  1461. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1462. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1463. adding koazak sequence to primer - (reply: 1)
  1464. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1465. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1466. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1467. Primer design if sequence is unknown for organism - (reply: 5)
  1468. adding C terminal tag to reverse primer - (reply: 1)
  1469. epitope tagging of pcr products - (reply: 1)
  1470. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1471. Real time PCR info - (reply: 4)
  1472. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1473. PCR from BAC - PCR from BAC (reply: 1)
  1474. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1475. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1476. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1477. How much product PCR?? - (reply: 3)
  1478. what primers should I use for DNA sequencing? please help - (reply: 2)
  1479. what primers should I use for DNA sequencing?? - (reply: 3)
  1480. Is my primer going to work for qRT-PCR? - (reply: 6)
  1481. Standard curve for RT-PCR - (reply: 1)
  1482. PCR using genomic DNA - (reply: 2)
  1483. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1484. Problem amplifying plasmid - (reply: 1)
  1485. Sybr Green I in real-time PCR reaction - (reply: 3)
  1486. Primers annealing temperatures - (reply: 8)
  1487. Difficulties cloning by PCR - (reply: 5)
  1488. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1489. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1490. high Cp value PCR - (reply: 2)
  1491. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1492. Synthesize own Oligo-dT primers - (reply: 6)
  1493. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1494. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1495. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1496. Smearing/ no amplification in PCR - (reply: 3)
  1497. primer design - Primer design for PCR (reply: 1)
  1498. How are primers designed? - (reply: 4)
  1499. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1500. DNA methylated PCR - (reply: 2)
  1501. need help on primer calcuation soon - (reply: 1)
  1502. primer design - (reply: 6)
  1503. problem in pcr and purification - (reply: 3)
  1504. Inability to duplicate PCR results - (reply: 8)
  1505. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1506. PCR using xt5 - (reply: 2)
  1507. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1508. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1509. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1510. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1511. 23S rRNA Primer - need primer sequence (reply: 1)
  1512. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1513. RT-PCR - problems with TaqMan PCR (reply: 2)
  1514. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1515. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1516. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1517. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1518. Small product amplification problems - (reply: 4)
  1519. multi way ligation or long range pcr? - (reply: 1)
  1520. Freezing primers+SYBR green - (reply: 2)
  1521. Tagged Primers - (reply: 5)
  1522. no pcr amplification product - (reply: 9)
  1523. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1524. design primer for chip - (reply: 5)
  1525. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1526. PCR problem - (reply: 4)
  1527. differently behavioring replicates in real-time PCR - (reply: 1)
  1528. Multiplex PCR - (reply: 1)
  1529. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1530. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1531. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1532. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1533. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1534. PCR components storage - (reply: 3)
  1535. reconstitution of primers - (reply: 2)
  1536. No PCR bands with some templates - (reply: 5)
  1537. Mysterious PCR Contamination - (reply: 13)
  1538. BS PCR..please help - DNA methylation analysis (reply: 15)
  1539. RT-PCR Help - Protocol provided (reply: 4)
  1540. PCR product purification - (reply: 3)
  1541. Mutagenesis PCR and undesired amplification - (reply: 2)
  1542. Temp Gradient PCR - (reply: 2)
  1543. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1544. PCR with genomic dna - (reply: 9)
  1545. primer dimer - (reply: 2)
  1546. PCR with long primers - (reply: 3)
  1547. BSP PCR standardization...help - (reply: 8)
  1548. PCR on degraded templates - (reply: 2)
  1549. PCR help needed - (reply: 3)
  1550. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1551. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1552. light bands, dark smear, dark dimers - (reply: 2)
  1553. PCR, followed by sequencing... - why ?? (reply: 3)
  1554. PCR program - strange extension step (reply: 2)
  1555. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1556. Help me! How to design nested BSP primers? - (reply: 4)
  1557. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1558. Plasmid linearization by PCR - (reply: 4)
  1559. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1560. Using BLAST to check primers - (reply: 17)
  1561. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1562. pcr doesnt show any band for DNa walking - (reply: 8)
  1563. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1564. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1565. Effects of more cDNA in PCR??? - (reply: 1)
  1566. PCR primer - (reply: 2)
  1567. PCR product disappears after restriction digestion - (reply: 4)
  1568. Primer design - GC clamp - (reply: 8)
  1569. Real Time PCR - excluding Ct - (reply: 2)
  1570. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1571. Price of a real time PCR machine? - (reply: 4)
  1572. colony PCR - (reply: 8)
  1573. probe conc. of Asymmetric PCR - (reply: 1)
  1574. primer dimer or what? - (reply: 2)
  1575. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1576. Real Time PCR method comments - (reply: 2)
  1577. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1578. colony pcr - (reply: 4)
  1579. Primers and Taqman Probes mixture question - (reply: 1)
  1580. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1581. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1582. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1583. Colony PCR - (reply: 15)
  1584. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1585. no bands in my PCR - (reply: 9)
  1586. designing primers - (reply: 1)
  1587. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1588. purefication and amplification of serum free DNA - (reply: 1)
  1589. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1590. Bisulfite Sequencing PCR - (reply: 4)
  1591. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1592. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1593. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1594. mRNA PCR - (reply: 1)
  1595. pcr product quantification - (reply: 5)
  1596. Negative Control Primers for ChIP Assay - (reply: 3)
  1597. Magnesium in PCR - (reply: 1)
  1598. PCR Sample Prep - (reply: 4)
  1599. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1600. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1601. Bizarre Contamination in PCR - (reply: 7)
  1602. PCR Cloning-large primers - (reply: 4)
  1603. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1604. forward and reverse primer - (reply: 4)
  1605. Check the primers - (reply: 4)
  1606. PCR problem from transformed TOPO TA vector - (reply: 3)
  1607. DIG DNA PCR labeling problem - (reply: 6)
  1608. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1609. BSP primers - (reply: 2)
  1610. Various size of insert after colony screen by PCR - (reply: 2)
  1611. inverse PCR molecular bilogy - (reply: 3)
  1612. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1613. failed PCR on DNA extract from blood - (reply: 6)
  1614. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1615. Primer dimer issue in real time PCR - (reply: 21)
  1616. Leaky RT-PCR - (reply: 4)
  1617. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1618. PCR, RNA, Northern Blotting???? - (reply: 1)
  1619. bizzare PCR smear - why does science hate me (reply: 7)
  1620. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1621. PCR detection of SNP - (reply: 6)
  1622. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1623. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1624. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1625. Sub-cloning sticky-end PCR products - (reply: 2)
  1626. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1627. ChIP on chip amplification problems - (reply: 11)
  1628. Genotyping PCR - (reply: 3)
  1629. PCR screening of transformed bacterial colony - (reply: 6)
  1630. Is this standard valid? - pcr product as standards (reply: 5)
  1631. primer binding - (reply: 1)
  1632. weird ChIP primers! - (reply: 1)
  1633. PCR primers - (reply: 1)
  1634. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1635. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1636. amplification in negative control in RT PCR - (reply: 2)
  1637. miRNA real time PCR by SYBR green methods - (reply: 1)
  1638. Deletion PCR - (reply: 9)
  1639. diluting to final primer concentration - please help correct (reply: 1)
  1640. Smear problem with my new primer set - (reply: 2)
  1641. PCR ghost bands - (reply: 1)
  1642. Amplifying a gene using a degenerate primer - (reply: 4)
  1643. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1644. CP of real time PCR by LC480 - (reply: 1)
  1645. CHIP- Real Time PCR calculations - (reply: 1)
  1646. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1647. transgene copy number - using real time PCR (reply: 1)
  1648. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1649. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1650. Unspecific PCR - (reply: 7)
  1651. Real-Time PCR as a Microplate Reader - (reply: 1)
  1652. gene-specific RT-PCR - (reply: 4)
  1653. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1654. RT-PCR Internal Standard - (reply: 2)
  1655. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1656. Plasmid supercoiling affecting PCR? - (reply: 2)
  1657. RNA contamination in PCR - (reply: 1)
  1658. PCR without DNA extraction! - (reply: 2)
  1659. Primer tm - (reply: 1)
  1660. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1661. re-amplification in real-time PCR - (reply: 1)
  1662. primer design - really necessary? (reply: 2)
  1663. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1664. RT-PCR - (reply: 3)
  1665. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1666. Degenerative primers = multiple products? - (reply: 5)
  1667. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1668. PCR problems on a ligation product - (reply: 5)
  1669. Genomic DNA and PCR - (reply: 4)
  1670. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1671. Sewing PCR - (reply: 3)
  1672. on the minimal pcr derived product - (reply: 2)
  1673. primer dimers - (reply: 5)
  1674. RT-PCR vs. conventional PCR - (reply: 1)
  1675. qPCR primer design - (reply: 5)
  1676. PCR and gel purification problem - did I screw this up (reply: 4)
  1677. Cloning PCR fragment - (reply: 1)
  1678. touchdown pcr - (reply: 6)
  1679. non specific pcr products - (reply: 4)
  1680. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1681. Anyone with experiences in 2 step PCR - (reply: 1)
  1682. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1683. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1684. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1685. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1686. re-amplification of a PCR product - is it recommended? (reply: 7)
  1687. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1688. PCR machine with accurate temperature control? - (reply: 1)
  1689. Gene-specific RT-PCR - (reply: 2)
  1690. Primer stock confusion - (reply: 6)
  1691. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1692. primer design - primer design question (reply: 2)
  1693. Protocol for 16S rDNA real time PCR - (reply: 2)
  1694. Touchdown PCR - (reply: 1)
  1695. Determine annealing temperature of primers - (reply: 14)
  1696. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1697. polymerase for BSP - (reply: 2)
  1698. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1699. Validating real time pcr primers - (reply: 2)
  1700. BSP,MSP primer design? - (reply: 1)
  1701. low gc primers - help with pcr using low gc primers (reply: 6)
  1702. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1703. Primers and annealing temperature - (reply: 2)
  1704. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1705. Copy number calculations in real time PCR - (reply: 2)
  1706. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1707. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1708. RT-PCR Negative controls - (reply: 1)
  1709. WHich primer to use for sequencing - (reply: 5)
  1710. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1711. minimum length for the gene to be amplified in PCR - (reply: 6)
  1712. dNTP Quantity - (reply: 3)
  1713. annoying PCR cloning problem (season 2) - (reply: 5)
  1714. PCR with 60 bp primers - results in no product (reply: 6)
  1715. Amplification dwindles using little template RT-PCR - (reply: 3)
  1716. Running gel after RT-PCR - (reply: 5)
  1717. Degenerate PCR Size Limit? - (reply: 3)
  1718. primer design for pBAD topo expression kit - (reply: 2)
  1719. PCR problems.. - problems with conventional PCR (reply: 3)
  1720. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1721. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1722. small PCR products on agarose gel? - (reply: 8)
  1723. Dnase digestion and PCR - (reply: 4)
  1724. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1725. primers fpr sequencing - (reply: 4)
  1726. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1727. Need an UV260 RT-PCR instrument - (reply: 1)
  1728. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1729. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1730. PCR produces products of wrong size - (reply: 4)
  1731. PCR with pfu and degenerate primers (?) - (reply: 7)
  1732. a doubt about PCR gel purification - (reply: 4)
  1733. Draw PCR primer locations - PCR primer (reply: 4)
  1734. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1735. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1736. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1737. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1738. PCR+enhancer - (reply: 4)
  1739. Serious issues with RT-PCR - (reply: 7)
  1740. PCR [dNTP] - dNTP concentrations (reply: 2)
  1741. PCR issue - (reply: 2)
  1742. dNTP question - (reply: 3)
  1743. PCR - (reply: 1)
  1744. long PCR primer - (reply: 7)
  1745. Taqman rtPCR primer and probe design - (reply: 3)
  1746. sequencing problem after pcr - (reply: 4)
  1747. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1748. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1749. primer design - (reply: 3)
  1750. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1751. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1752. Analysis of ChIP RT-PCR data - (reply: 7)
  1753. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1754. Negative flouroscence in real time PCR - (reply: 2)
  1755. Minimum number of PCR cycles to see a product? - (reply: 6)
  1756. decontamination - decontamination for PCR (reply: 6)
  1757. Troubleshoot ARMS PCR - (reply: 2)
  1758. negative control for PCR - (reply: 1)
  1759. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1760. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1761. pcr pdt amplification - (reply: 1)
  1762. real time PCR - (reply: 3)
  1763. PCR fails when I scale up - (reply: 2)
  1764. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1765. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1766. PCR not Working = SMEAR - (reply: 3)
  1767. How to do PCR fragment in 2 steps - (reply: 3)
  1768. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1769. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1770. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1771. RT-PCR - problem of PCR (reply: 1)
  1772. PCR product longer than template - why? - (reply: 2)
  1773. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1774. Dye recipe that can be used for PCR MM - (reply: 3)
  1775. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1776. PCR conditions with three primers - (reply: 1)
  1777. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1778. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1779. Cloning a labeled PCR product - (reply: 1)
  1780. PCR and cloning - (reply: 14)
  1781. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1782. PCR product - not suppose to be there (reply: 7)
  1783. using the meth primer - (reply: 2)
  1784. Generating primers for MSP - (reply: 1)
  1785. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1786. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1787. PCR from a smear genomic DNA ? - (reply: 5)
  1788. Primer design and need help - (reply: 11)
  1789. Setting up a multiplex PCR assay. - (reply: 2)
  1790. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1791. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1792. cDNA and RT-PCR - (reply: 8)
  1793. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1794. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1795. PCR problem - help me plsss (reply: 9)
  1796. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1797. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1798. PCR efficiencies - (reply: 4)
  1799. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1800. PCR quantification - (reply: 1)
  1801. PCR quantification - (reply: 1)
  1802. Long PCR product - (reply: 9)
  1803. problem in Pcr amplification - (reply: 7)
  1804. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1805. difference between hotstart and taq polymerase - (reply: 7)
  1806. sequencing the pcr product - (reply: 15)
  1807. Pcr amplification - primer designed from UTR regions (reply: 5)
  1808. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)