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PCR Related Discussions
  1. RT-PCR low efficiency - (reply: 1)
  2. emulsion PCR - (reply: 1)
  3. RT-PCR electrophorasis - (reply: 1)
  4. PCR contamination - (reply: 3)
  5. PCR bands on gel - (reply: 5)
  6. Problem with restriction digestion of cloned PCR product - (reply: 5)
  7. SYBR Green Real time PCR - (reply: 2)
  8. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
  9. need help calculating stock primer concentration - (reply: 1)
  10. High background fluorescence detected before starting PCR - (reply: 3)
  11. How to clean up PCR place & pipettes? - (reply: 1)
  12. Real-time pcr - (reply: 1)
  13. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
  14. PCR inhibitors - (reply: 1)
  15. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
  16. Polymerase mixture for blunt ended fragments - (reply: 2)
  17. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  18. Amplifying shRNA lentivirus - (reply: 1)
  19. Mineral oil quality in PCR tubes - (reply: 5)
  20. Diagnostic PCR troubleshooting - (reply: 2)
  21. RT-PCR Kit's Validation - (reply: 1)
  22. Rt pcr - (reply: 4)
  23. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
  24. methylation specifc primers thermal cycle conditions - (reply: 4)
  25. PCR Amplification Issues and Primer dimer - (reply: 4)
  26. How to make PCR analysis using the 22DDCT Method - (reply: 1)
  27. Primer calculation for qPCR - final reaction - (reply: 1)
  28. Sec. structure in primers - (reply: 1)
  29. Changing PCR recipe halfway - (reply: 1)
  30. PCR master mix containing primers and Taq - (reply: 4)
  31. Primer working with some cDNA samples and not other - (reply: 2)
  32. no bands after pcr!!!! - (reply: 4)
  33. Experimental design for RT-PCR - (reply: 8)
  34. Primer dilution - (reply: 7)
  35. why not working msp primers - (reply: 4)
  36. PCR product not migrating from wells...?? - (reply: 6)
  37. Re-PCR my PCR products - (reply: 2)
  38. PCR DIG-labeling kit preblem? - (reply: 1)
  39. pcr - (reply: 1)
  40. how to analysis the BiSearch ePCR result - (reply: 3)
  41. Standardizing cDNA concentration before doing PCR - (reply: 4)
  42. Volume of Primers - (reply: 4)
  43. PCR conditions unknown? - (reply: 10)
  44. PCR anomaly in a mid-range dilution of the template - (reply: 2)
  45. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
  46. MSP_Primer dimers? - (reply: 17)
  47. PCR using very long oligos !!! - (reply: 4)
  48. Primer Designing - (reply: 9)
  49. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  50. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  51. colony pcr, two strong bands - (reply: 4)
  52. Ligation of two PCR products - (reply: 1)
  53. pcr primer design - (reply: 6)
  54. Please help (primer/ probe final volume calculations) - (reply: 1)
  55. Primer design with EcoRI ends - (reply: 2)
  56. Amplification problem in DNA isolated after ChIP experiment, - (reply: 1)
  57. Trouble with PCR using ligation mix as template? - (reply: 3)
  58. PCR with mammalian cells as template - (reply: 1)
  59. PCR with overlapping primers only (primer extention) - (reply: 3)
  60. Random chuck of nonsense in middle of PCR - (reply: 6)
  61. RT-PCR analysis - (reply: 1)
  62. PCR DNA bands too large on agarose gel - (reply: 1)
  63. MSP Primer design - (reply: 5)
  64. New to PCR - (reply: 3)
  65. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  66. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
  67. Primer design - (reply: 1)
  68. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)
  69. Primer non specific binding sites - (reply: 7)
  70. Degenerate primers - (reply: 8)
  71. Primers with 2 different annealing temp - (reply: 1)
  72. Can you use same samples for real time pcr 2nd time? - (reply: 2)
  73. Staphylococcus aureus colony PCR - (reply: 1)
  74. Cloning purified PCR product into cells - (reply: 5)
  75. PCR & Western Blot sample preperation - (reply: 2)
  76. PCR & Western Blot sample preperation - (reply: 5)
  77. Y-linker transgene integration site PCR primer verification - (reply: 1)
  78. Non especific band in PCR... - (reply: 2)
  79. I need help with PCR problems - (reply: 5)
  80. Question about baselines in Real-Time PCR - (reply: 3)
  81. Inconsistent colony PCR result - (reply: 2)
  82. A problem with PCR array - (reply: 4)
  83. My PCR suddenly stopped working and I'm losing my mind - (reply: 3)
  84. Concentration of Eva green with different amplicon sizes in a PCR pool - (reply: 1)
  85. labX offers on realtime PCR machines - (reply: 4)
  86. Could any of you help with an explanation to these vague real time PCR curves &# - (reply: 2)
  87. polymerase with or without proofreading activity used in study nucleotide polymo - (reply: 1)
  88. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  89. Suggest best micropipette for PCR purpose... - (reply: 1)
  90. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  91. Plasmid Amplification Issue - (reply: 6)
  92. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  93. primer design for a gene - (reply: 3)
  94. large bands in all pcr reactions including negative control - (reply: 1)
  95. confirmation for primers dilution - (reply: 10)
  96. Problems regarding amplification of my gene - (reply: 3)
  97. PCR working, qPCR is *not*. - (reply: 6)
  98. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  99. Overlapping PCR, need help - (reply: 5)
  100. How does RT-PCR work? - (reply: 5)
  101. Denatured plasmid for pcr - (reply: 1)
  102. Possible primer dimer problem..Need solution!!! - (reply: 2)
  103. confusing with where to put primers and which ordination - (reply: 4)
  104. Help with primer concentration for sequencing - (reply: 1)
  105. PCR troubleshoot - (reply: 6)
  106. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  107. PCR Products not run well in the GEL - (reply: 1)
  108. PCR solution calculus - (reply: 4)
  109. Amplifying from pcr products - (reply: 7)
  110. Consistancy issue with PCR block - (reply: 1)
  111. Queries regarding PCR - (reply: 3)
  112. Design the primers - (reply: 10)
  113. Calculation of Taq Polymerase - (reply: 3)
  114. Best Taq for colony PCR? - (reply: 5)
  115. How two fragments joint together when doing fusion PCR? - (reply: 4)
  116. How to test my milleq water in q PCR about contaminants - (reply: 3)
  117. Mutagenesis PCR - (reply: 9)
  118. real time PCR primer design - (reply: 1)
  119. The PCR gods are frowning upon me - (reply: 9)
  120. PCR after T4 ligation? - (reply: 3)
  121. primers for cloning - (reply: 1)
  122. Degenerated Primers and their problems - (reply: 1)
  123. Sonication of small PCR amplicon - (reply: 1)
  124. primer design with restriction enzyme - (reply: 1)
  125. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  126. primer dilution help - (reply: 1)
  127. Testing primers on 'unknown' tissue - (reply: 1)
  128. How to remove inhibitory substances in PCR? - (reply: 2)
  129. need some advise about PCR of PKD1-human gene - (reply: 7)
  130. puzzled with PCR outcome after BS treatment - (reply: 2)
  131. nested PCR for low viral load- HBV patient sample - (reply: 2)
  132. troubleshooting stubborn PCR - (reply: 6)
  133. Measuring pcr fragments in a gel - (reply: 1)
  134. Amplification in HK gene but not for target gene - (reply: 3)
  135. could you help me with my stem loop RT primer? - (reply: 1)
  136. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  137. How long can I store at - 30°C my PCR mix? - (reply: 6)
  138. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  139. RT-PCR help - (reply: 2)
  140. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  141. PCR Temperature change control malfunctioning - (reply: 1)
  142. excess amount of primers - (reply: 2)
  143. Dehydrating and Reconstituting primers - (reply: 15)
  144. Trouble with PCR of short sequence - (reply: 3)
  145. Setting the temperature range for gradient PCR - (reply: 3)
  146. KIR gene promoters for MSP primers design - (reply: 2)
  147. Biotin-streptavidin signal amplification - (reply: 2)
  148. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  149. Amplification curve in the negative control samples - (reply: 7)
  150. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  151. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  152. Electrophoresis after PCR : too many bands - (reply: 6)
  153. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  154. Primer as limiting reagent in PCR reaction - (reply: 2)
  155. Primer Design for RNA probes - (reply: 2)
  156. Real time PCR for degraded RNA - (reply: 1)
  157. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  158. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  159. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  160. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  161. Nested BSP Primer Design - (reply: 6)
  162. website for primer design - (reply: 1)
  163. PCR Efficiency over 150%! - (reply: 1)
  164. problem of amplification - (reply: 2)
  165. PCR not working - (reply: 11)
  166. How to do a primer dilution - (reply: 10)
  167. Troubles with Fusion PCR - (reply: 1)
  168. Primer design and alternative transcripts - (reply: 2)
  169. PCR and sequencing of genomic DNA - (reply: 5)
  170. qPCR amplification - (reply: 4)
  171. NESTED PCR - (reply: 6)
  172. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  173. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  174. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  175. Digestion necessary after PCR? - (reply: 9)
  176. Inverse PCR product selection - (reply: 2)
  177. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  178. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  179. how to hasten real-time PCR amplifications - (reply: 2)
  180. Please please help me with my Phusion PCR. - (reply: 5)
  181. PCR - (reply: 1)
  182. NCBI Primer Design - Stringency Issues - (reply: 3)
  183. Different primer concentration in qPCR - (reply: 1)
  184. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  185. help in long pcr - (reply: 1)
  186. PCR inhibitor in template DNA - (reply: 3)
  187. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  188. Problem with Real-time PCR results analysis - (reply: 1)
  189. Primer Specificity: Testing only one primer - (reply: 4)
  190. PCR from protozoa DNA - (reply: 3)
  191. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 7)
  192. tool for comparing many primers pairs - (reply: 4)
  193. PCR that leads to protein synthesis - (reply: 18)
  194. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  195. Use of DMSO in General PCR - (reply: 1)
  196. PCR product size confusion - (reply: 3)
  197. Pcr primers - (reply: 7)
  198. Concentration specification in PCR - (reply: 3)
  199. Guanidine isothiocyanate in PCR - (reply: 1)
  200. Primers have worked well but now getting primer dimers? - (reply: 2)
  201. I cannot design primers on exon-exon junction - (reply: 2)
  202. DNA Quantification of PCR Products - (reply: 2)
  203. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  204. Problem for PCR - (reply: 9)
  205. Designing primers in UTRs - (reply: 1)
  206. Multiplex PCR - (reply: 1)
  207. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  208. primer design@ buy? - (reply: 2)
  209. Trouble with overlap extension pcr - (reply: 3)
  210. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  211. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  212. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  213. Question about the RT PCR - (reply: 3)
  214. faint dna band after pcr purification - (reply: 1)
  215. PCR ready mixes with long shelf lives - (reply: 4)
  216. Troubleshooting: Inverse PCR - (reply: 3)
  217. How to design primer to amplify genomic DNA? - (reply: 3)
  218. Overlap PCR, need help - (reply: 11)
  219. Internal control for miRNA RT-PCR - (reply: 1)
  220. Primers - (reply: 1)
  221. Primers mix - (reply: 2)
  222. question about RNA concentration for real time PCR - (reply: 1)
  223. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  224. PCR products sizes and DNA ladder - (reply: 7)
  225. Having problem with primers for qPCR - (reply: 4)
  226. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  227. PCR product sequencing - (reply: 3)
  228. Bad fragment amplification - (reply: 4)
  229. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  230. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  231. No amplification with TRAPEZE kit! - (reply: 1)
  232. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  233. Stargazer PCR problems - (reply: 3)
  234. General PCR discussion - (reply: 8)
  235. Real time. No amplification but hight flourescence - (reply: 1)
  236. Real time PCR doubt - (reply: 5)
  237. PCR Profile for ligation - (reply: 3)
  238. protocol to relieve melanin inhibition of PCR - (reply: 4)
  239. No bands despite different primers and conditions and TAqs - (reply: 8)
  240. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  241. Equimolar Mix Primer - (reply: 8)
  242. Wrong PCR product - (reply: 2)
  243. Colony PCR positive and Digestion negative????? - (reply: 11)
  244. Primer design - (reply: 5)
  245. storage for lyophilized primers - (reply: 1)
  246. PCR GAPDH gene - (reply: 1)
  247. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  248. Quantitative RT-PCR statistics help - (reply: 1)
  249. Dimerization of PCR product - (reply: 4)
  250. PCR Primer trouble - (reply: 2)
  251. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  252. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  253. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  254. finding the corrosponding primers - (reply: 2)
  255. Gene sequence for Real time PCR - (reply: 2)
  256. non-reproducible PCR results with cDNA as template - (reply: 1)
  257. Ligation of PCR fragments - (reply: 11)
  258. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  259. RNA extraction for RT-PCR - (reply: 3)
  260. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  261. problems with gDNA doing real time PCR in yeast - (reply: 2)
  262. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  263. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  264. How to get rid of bands in PCR negative control - (reply: 10)
  265. T7 and M13 primers two band amplification - (reply: 3)
  266. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  267. Strange PCR problem - (reply: 2)
  268. Question about Double Digestion followed by PCR amplification - (reply: 2)
  269. PCR problem - (reply: 2)
  270. PCR machine not working properly - (reply: 1)
  271. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  272. PCR gene specific amplification problem - (reply: 3)
  273. Failure SYBRGREEN PCR - (reply: 4)
  274. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  275. without RE site in PCR product - (reply: 5)
  276. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  277. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  278. real-time pcr non reproducible - (reply: 4)
  279. Strange amplification plots with high Ct variability - (reply: 1)
  280. How big a role does mixing play in PCR - (reply: 1)
  281. Melting curve is irregular for primer optimization - (reply: 5)
  282. Designing primers for ABO blood groups - (reply: 1)
  283. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  284. RT-PCR primer design - (reply: 7)
  285. How to amplify very short PCR template - (reply: 4)
  286. Is this primer okay? - (reply: 4)
  287. PCR amplification of large template - (reply: 1)
  288. PCR insert - in frame? - (reply: 2)
  289. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  290. smear in gel electrophoresis after PCR - (reply: 2)
  291. Can I use Primer as template? - (reply: 1)
  292. Whole plasmid amplification by PCR - (reply: 2)
  293. PCR product as standard curve template - (reply: 6)
  294. colony PCR after transformation - (reply: 1)
  295. PCR machine - (reply: 3)
  296. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  297. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  298. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  299. Resuspending primers calculation - (reply: 4)
  300. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  301. PCR RFLP is PCR product purification necessary? - (reply: 3)
  302. Mystery in my PCR - (reply: 5)
  303. Whole genome amplification from cDNA? - (reply: 4)
  304. What do you use to check primer secondary structure? - (reply: 3)
  305. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  306. PCR product as template for in vitro transcription - (reply: 1)
  307. Problem with 3 step PCR - (reply: 3)
  308. mutagenic primers with very high GC content. - (reply: 3)
  309. Scaling up PCR to get more DNA - (reply: 5)
  310. PCR to get 10kbp product - (reply: 4)
  311. site-directed mutagenesis primer Tm - (reply: 4)
  312. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  313. Random vs oligo primer in preamplification RT - (reply: 1)
  314. how does polymerase stop at the required length - (reply: 1)
  315. Chip pcr. are there inespecifics? - (reply: 1)
  316. Primer concentration - stupid question - (reply: 3)
  317. Primer Design, help i´m New - (reply: 4)
  318. No DNA after PCR product purification - (reply: 9)
  319. Sensitivity of RT-PCR and qPCR - (reply: 4)
  320. RT-qPCR primer problem - (reply: 4)
  321. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  322. real time PCR trouble - (reply: 3)
  323. removal of ethanol in PCR product purification - (reply: 5)
  324. Crazy real time PCR curve - (reply: 4)
  325. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  326. High fidelity PCR trouble shooting - (reply: 2)
  327. Designing cloning primers for DNMT - (reply: 2)
  328. Reproducible Non-Specific PCR Product - (reply: 2)
  329. Are these primer products good enough for qPCR? - (reply: 3)
  330. Can't get PCR with large overhang primers to work - (reply: 8)
  331. Primer Efficiency across runs - (reply: 1)
  332. Question about pipet tips for PCR and rtPCR - (reply: 4)
  333. UNG in PCR - (reply: 1)
  334. Cause of random samples failing PCR? - (reply: 2)
  335. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  336. pcr 10X buffer preparation - (reply: 2)
  337. how much template do i need for pcr? - (reply: 5)
  338. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  339. Problems with crossover PCR - (reply: 2)
  340. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  341. Designing PCR Primers for cloning - (reply: 17)
  342. Creating primers to add restriction sites to vector backbone - (reply: 7)
  343. PCR DNA Concentration - (reply: 1)
  344. RT-PCR product- no band - (reply: 4)
  345. Some primers dose not work - (reply: 5)
  346. Understanding RACE PCR - (reply: 1)
  347. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  348. Wierd Bands after PCR....Confused - (reply: 9)
  349. Query regarding primers for quick change mutagenesis - (reply: 3)
  350. PCR with Plasmid recovered from filter paper - (reply: 6)
  351. PCR amplified product size - (reply: 5)
  352. the storage time for primers - (reply: 9)
  353. Annealing temperature for PCR - (reply: 8)
  354. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  355. Nested PCR - (reply: 2)
  356. random primers or oligodT - (reply: 4)
  357. No amplification during RT-PCR - (reply: 4)
  358. Sequencing RT-PCR product - (reply: 3)
  359. Low yield PCR product after gel purification - (reply: 8)
  360. To design or use published primers? - (reply: 4)
  361. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  362. PCR primer usage (Clonning & cDNA) - (reply: 2)
  363. problem in cloning PCR primer design with restriction site - (reply: 4)
  364. Amplification with U primers, but not with M primers - (reply: 1)
  365. Taq polymerase - (reply: 7)
  366. His tag introduction in to gene and primer design - (reply: 2)
  367. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  368. Which High-Fidelity polymerase is better? - (reply: 2)
  369. polymerase to use for cloning - (reply: 4)
  370. Problem amplifying viral gene - (reply: 5)
  371. Adding tag using overlapping PCR - (reply: 2)
  372. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  373. Design primer from incomplete sequence... - (reply: 2)
  374. Design primer from incomplete sequence... - (reply: 2)
  375. How to perform colony PCR - (reply: 1)
  376. Primer designing for Methylation - (reply: 6)
  377. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  378. cDNA amplification problem - (reply: 4)
  379. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  380. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  381. PCR product running on agarose gel - (reply: 32)
  382. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  383. No bands in PCR after DNase treatment - (reply: 4)
  384. PCR amplification with high fidelity enzyme - (reply: 1)
  385. PCR HELP!!!! - (reply: 1)
  386. Overlap PCR problem - (reply: 5)
  387. PCR and restriction enzyme digestion - (reply: 3)
  388. Bisulfite PCR and cloning - (reply: 5)
  389. PCR product purification - (reply: 4)
  390. Overlapping sequence PCR primers - (reply: 1)
  391. restricted PCR plasmid runs slower - (reply: 2)
  392. BSP PCR primer design explained - (reply: 11)
  393. Bisulfite sequencing PCR worked - (reply: 5)
  394. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  395. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  396. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  397. doing PCR using PCR product , help plz !! - (reply: 5)
  398. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  399. good 16S univ. primer for qPCR? - (reply: 3)
  400. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  401. Amplification of CAG promoter problems - (reply: 4)
  402. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  403. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  404. I need help for designing a pair of primers for this sequence. - (reply: 1)
  405. PCR not working for 5 Aza treated DNA - (reply: 3)
  406. Differentiating mouse from human cells with PCR - (reply: 5)
  407. Differentiate between RT-PCR and PCR - (reply: 3)
  408. PCR band slightly BELOW expected length ?! - (reply: 14)
  409. long primers PCR - (reply: 4)
  410. unexpected band in PCR with plasmid - (reply: 4)
  411. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  412. DNA polymerase for GC rich template? - (reply: 5)
  413. Sequencing Primers and Plasmids - (reply: 3)
  414. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  415. DNA extraction - PCR Problem - (reply: 3)
  416. Primers to amplify Kan operon from pet28 - (reply: 2)
  417. PCR product biotinylation - (reply: 3)
  418. Tagged primers and a second PCR targetting the tags - (reply: 5)
  419. cloning pcr insert into plasmid - (reply: 2)
  420. Barcode generator for PCR primers - (reply: 2)
  421. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  422. Barcoding PCR products - (reply: 18)
  423. Biotinylated primers - (reply: 4)
  424. role of water in PCR - (reply: 3)
  425. HIV gene- PCR - (reply: 3)
  426. Real time PCR melting curve - (reply: 2)
  427. BSA in PCR reaction - (reply: 1)
  428. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  429. Cannot see insert with colony pcr - (reply: 6)
  430. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  431. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  432. How to Join two PCR products - (reply: 3)
  433. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  434. What's wrong with my PCR - (reply: 5)
  435. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  436. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  437. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  438. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  439. Cp, but no amplification - (reply: 2)
  440. pcr product digestion problem - (reply: 4)
  441. Low PCR product - (reply: 7)
  442. Control primers for bisulfite-converted DNA - (reply: 3)
  443. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  444. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  445. wrong dntp concentration - (reply: 2)
  446. Third round PCR - (reply: 2)
  447. long fragment PCR - (reply: 2)
  448. Best proof-reading polymerase? - (reply: 13)
  449. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  450. Q regarding primer mix for MSP!! - (reply: 4)
  451. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  452. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  453. I need to break this cycle of PCR issues - (reply: 13)
  454. Questions / Help with Fusion PCR - (reply: 8)
  455. Controls for RT-PCR reactions - (reply: 2)
  456. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  457. BSP Primers with M13 Tails - (reply: 1)
  458. Detection of T7 RNA polymerase by WB - (reply: 6)
  459. Multiplex PCR - (reply: 33)
  460. issues when designing PCR primers using PRIMER3 - (reply: 8)
  461. Primer catalog/databasing - (reply: 1)
  462. Nonrepetitive nested PCR - (reply: 2)
  463. Primer Blast - (reply: 6)
  464. Help designing primers for use in In-fushion cloning - (reply: 4)
  465. PCR internal control - (reply: 2)
  466. Primers Freeze-Thaw - (reply: 16)
  467. TDNA Genotyping PCR program - (reply: 1)
  468. primer design using 16s rRNA - (reply: 1)
  469. question about PCR and cloning - (reply: 1)
  470. PCR - (reply: 2)
  471. nonspecific band at 50bp in PCR - (reply: 9)
  472. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  473. PCR master mix - (reply: 7)
  474. Maximum PCR product Tm - (reply: 6)
  475. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  476. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  477. PCR & Southern blotting - (reply: 1)
  478. Bisulfite PCR Question - (reply: 2)
  479. Primer Design HELP! - (reply: 3)
  480. Help with sequencing of a 120bp PCR product. - (reply: 9)
  481. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  482. How are Primers made? - (reply: 9)
  483. Long Non-Specific PCR Products - (reply: 5)
  484. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  485. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  486. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  487. PCR calculation - (reply: 1)
  488. Colony PCR and digestion with KpnI - (reply: 10)
  489. designing methylation specific primers - (reply: 3)
  490. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  491. PCR - consistent false positive results - (reply: 6)
  492. PCR no amplification - (reply: 15)
  493. How to quantify virus by PCR - (reply: 12)
  494. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  495. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  496. Pcr product size determination for a fusion gene - (reply: 8)
  497. Quantify DNA before ChIP PCR - (reply: 3)
  498. Primer Annealing Temperatures - (reply: 24)
  499. my pcr product is larger than expected - (reply: 4)
  500. Question about PCR - DNA or RNA as template - (reply: 4)
  501. pcr purification - (reply: 1)
  502. pcr purification doubt - (reply: 4)
  503. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  504. PCR band moves lower than its size on agarose gel - (reply: 3)
  505. same primers, different product in conventional PCR and qPCR - (reply: 6)
  506. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  507. pcr problem - (reply: 6)
  508. NEB Q5 polymerase works very well - (reply: 1)
  509. Primers amplify genomic DNA but not cDNA - (reply: 2)
  510. pcr purification - (reply: 1)
  511. storing PCR product overnight - (reply: 2)
  512. Primer BLAST - (reply: 1)
  513. Bands in negative control PCR - (reply: 2)
  514. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  515. problem with pcr mutagenesis - (reply: 10)
  516. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  517. Use PCR purification kit to purify restriction digestion - (reply: 2)
  518. PCR primer design - published primers trustable? - (reply: 8)
  519. Polymerase for E. coli screenings - (reply: 5)
  520. complicated RT-PCR issues - (reply: 4)
  521. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  522. Can we use degenerate bases in BSP primers? - (reply: 1)
  523. Quick change mutagenesis No PCR product visible - (reply: 2)
  524. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  525. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  526. Can anyone help with my Bisulfite PCR? - (reply: 2)
  527. How to check primer sequences using BLAST and other tools? - (reply: 2)
  528. real time PCR analysis in patient samples - (reply: 2)
  529. carpet in gels for PCR products - (reply: 2)
  530. Primer Check? - (reply: 2)
  531. Sequencing of the PCR production in BSP - (reply: 1)
  532. amplification of 14Kb - (reply: 1)
  533. Validation of PCR primers/ probes - (reply: 6)
  534. Taq polymerase for MSP - (reply: 1)
  535. Real time PCR sudenly not working - (reply: 1)
  536. Buffer-composition One-Step RT-PCR - (reply: 4)
  537. Two reverse primer sequences for a single forward primer - (reply: 1)
  538. PCR with more than one primer - (reply: 1)
  539. RAPD - PCR problem - (reply: 5)
  540. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  541. How to best store PCR product? - (reply: 2)
  542. RT-PCR primer design - (reply: 1)
  543. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  544. Odd gel run following PCR - (reply: 28)
  545. Qiagen PCR Array Reagents? - (reply: 1)
  546. No or very weak band using 16s primers - (reply: 1)
  547. No PCR amplification with b-actin primers - (reply: 1)
  548. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  549. Site-directed, PCR works but no colonies - (reply: 2)
  550. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  551. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  552. No PCR amplified with long primers - (reply: 10)
  553. PCR optimization: PCR vs qPCR - (reply: 4)
  554. should I do blunt end my pcr product before ligation - (reply: 2)
  555. PCR smear for genomic samples - (reply: 4)
  556. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  557. another primer dilution question - (reply: 2)
  558. i need help with virus pcr - (reply: 2)
  559. Primer Efficiency - (reply: 2)
  560. Experimental set up for qRT PCR - (reply: 13)
  561. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  562. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  563. How should I optimize my PCR - (reply: 2)
  564. Second round of PCR after gel extraction fails miserably - (reply: 3)
  565. an effective way to do a yeast colony pcr - (reply: 2)
  566. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  567. urgent help plz with RT-PCR - (reply: 3)
  568. oligo(dt) 15 vs random primers - (reply: 3)
  569. Help to optain a long PCR produc - (reply: 3)
  570. Multiple bands in eluted PCR product - (reply: 1)
  571. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  572. Problems with two step RT-PCR - (reply: 2)
  573. Getting genomic DNA for PCR - (reply: 4)
  574. primer dilutions - (reply: 4)
  575. Genotyping PCR primers - (reply: 1)
  576. Conventional PCR - (reply: 1)
  577. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  578. PCR product loss on cleanup - (reply: 2)
  579. MSP Primers not working - (reply: 6)
  580. Primers too dilute - (reply: 1)
  581. Amplification curves jagged - (reply: 1)
  582. Cloning HELP- Possible Primer issues - (reply: 6)
  583. No PCR product in site-directed mutagenesis - (reply: 4)
  584. Giardia/Crypto real time PCR late amplification - (reply: 1)
  585. How to dilute the template DNA for PCR - (reply: 7)
  586. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  587. Need help for PCR - (reply: 5)
  588. PCR primer pairs - university exam - (reply: 7)
  589. unespecific band PCR from mouse genomic DNA - (reply: 9)
  590. Help with primer design through PRIMER3 - (reply: 4)
  591. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  592. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  593. Plasmid vs cDNA amplification by PCR - (reply: 2)
  594. Tm of primers - prediction and verification - (reply: 1)
  595. PCR cloning...help! - (reply: 5)
  596. RT-PCR contamination in negative control. - (reply: 1)
  597. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  598. how to design primers for 16sr RNA - (reply: 24)
  599. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  600. Help with primer design - (reply: 3)
  601. Primer Tm calculation. - (reply: 1)
  602. low concentration of purified PCR product - (reply: 2)
  603. PCR smearing and...problems! - (reply: 7)
  604. Running Primers on Agarose Gel - (reply: 6)
  605. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  606. universal primers for PCR ribotyping - (reply: 6)
  607. will the template of PCR be added A ? - (reply: 2)
  608. Colony PCR not working - (reply: 4)
  609. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  610. Primer design: Tm above or below Ta? - (reply: 5)
  611. Oligo primers for shRNA construction - (reply: 1)
  612. Design primers for expression cloning - (reply: 11)
  613. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  614. primer dilution query - (reply: 2)
  615. Real Time PCR polymerase enzyme - (reply: 3)
  616. Estimating size of PCR products in a agarose gel - (reply: 4)
  617. Sequencing of PCR product - (reply: 4)
  618. PCR contamination problem - (reply: 2)
  619. Using BLAST to check primers - (reply: 1)
  620. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  621. What is the function of a third primer in a PCR mix?? - (reply: 2)
  622. How can we design a primer ? step by step method - (reply: 4)
  623. cDNA synthesis or RT-PCR - (reply: 3)
  624. PCR on cDNA and SGamplification??? - (reply: 1)
  625. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  626. real time PCR protocol - controls? - (reply: 2)
  627. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  628. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  629. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  630. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  631. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  632. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  633. PCR and UV spectrophotometry - (reply: 3)
  634. RT-PCR in a single tube - (reply: 3)
  635. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  636. annealing temperature for 3 primer pairs? - (reply: 4)
  637. PCR Reaction - (reply: 3)
  638. Impact of primer sequence on TA cloning - (reply: 3)
  639. problems with Bisulfite PCR - (reply: 1)
  640. low pcr product - (reply: 3)
  641. overlapping PCR protocol - (reply: 12)
  642. PCR to amplify my insert not working - (reply: 6)
  643. Problem with PCR amplification- high 3' stability - (reply: 3)
  644. Home made real-time PCR problem - (reply: 4)
  645. High RNase concentration interfering with PCR? - (reply: 8)
  646. Pcr primers - (reply: 2)
  647. PCR failure from yeast genomic DNA - (reply: 1)
  648. failure of PCR from yeast genomic DNA - (reply: 1)
  649. RT-Q PCR primer design question - (reply: 2)
  650. high MW dimers - (reply: 2)
  651. Strange RT-PCR amplification plots - (reply: 9)
  652. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  653. What products will I get from this pcr? - (reply: 1)
  654. PCR buffer without Tris - (reply: 1)
  655. PCR Water - (reply: 4)
  656. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  657. Which Brand of real-time PCR machine - (reply: 6)
  658. Problem with PCR - large extra bands - (reply: 3)
  659. big differnece in my primers Tm - (reply: 2)
  660. Designing primers for the ORFs - (reply: 9)
  661. Two-Step PCR - (reply: 2)
  662. Gradient PCR - (reply: 2)
  663. PCR with phophorylated primers - (reply: 1)
  664. Problem with New qPCR primers - help! - (reply: 6)
  665. Designing primers for miRNA located on minus strand. - (reply: 2)
  666. Plasmid sequencing without primers - (reply: 1)
  667. Smallest insert in PCR - (reply: 6)
  668. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  669. loop-mediated amplification - (reply: 2)
  670. Blunt end PCR product ligation - (reply: 1)
  671. Help with high Ct values for Real-Time PCR - (reply: 1)
  672. Genotyping by allele specific PCR - (reply: 6)
  673. RT-PCR primers and the poly(A) tail - (reply: 2)
  674. NO Insert found in the Colony PCR - (reply: 15)
  675. No band in PCR - (reply: 5)
  676. overlapping primer - (reply: 1)
  677. PCR primers help - (reply: 5)
  678. PCR smear after one-month storage - (reply: 2)
  679. Primer dimers - (reply: 2)
  680. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  681. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  682. Simple question of dNTP's for PCR - (reply: 5)
  683. Need help for PCR for AT rich gene - (reply: 3)
  684. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  685. Primer validation and PCR efficiency - (reply: 4)
  686. How to calculate the price of PCR test per sample? - (reply: 6)
  687. Contamination in negative control of PCR (No template control) - (reply: 6)
  688. cloning with nested pcr - (reply: 3)
  689. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  690. PCR is amplifying non-specific fragment - (reply: 6)
  691. ChIP amplification problems - (reply: 1)
  692. real time RT-PCR problems - (reply: 2)
  693. should I design primers spanning both intron and exon - (reply: 2)
  694. RT-PCR - (reply: 1)
  695. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  696. Template DNA (plasmid) concentration for PCR - (reply: 4)
  697. PCR with a very long and a short primer - (reply: 5)
  698. Ligation of 16kb PCR product - (reply: 5)
  699. Multiplex PCR does not work - (reply: 3)
  700. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  701. Problem with genomic PCR (2.4 kb) - (reply: 1)
  702. Comparison between two primers pairs? Is it possible? - (reply: 3)
  703. Rt-PCR primer design - (reply: 7)
  704. Length of non-binding sequence in primers with RE site - (reply: 6)
  705. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  706. Getting a smaller PCR product than required - (reply: 6)
  707. Difficult sequences in PCR - (reply: 2)
  708. why is real time pcr curve like this? - (reply: 3)
  709. PCR inconsistency - (reply: 2)
  710. Orientation of primers - (reply: 2)
  711. PCR, neg control & no. of cycles - (reply: 2)
  712. Strange PCR result from genomic DNA - (reply: 3)
  713. Bright bands of PCR products stick in gel wells - (reply: 1)
  714. methylation analysis with MS-HRM (primer design) - (reply: 1)
  715. whole plasmid pcr? - (reply: 6)
  716. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  717. salts in PCR - (reply: 4)
  718. Strange amplification plot - (reply: 4)
  719. Conventional PCR of different dilutions - (reply: 1)
  720. primer resuspension - (reply: 2)
  721. Principles of overlap PCR - (reply: 7)
  722. ligation of pcr product into expression vector - (reply: 4)
  723. Unable to PCR!!!! - (reply: 3)
  724. Help designing primers with restriction sites - (reply: 3)
  725. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  726. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  727. Abnormal amplification curve - (reply: 1)
  728. Problem with Semiquantitative PCR - (reply: 4)
  729. Advice on optimising bisulfite PCR sought - (reply: 2)
  730. Help: I am loosing the DNA during PCR purification - (reply: 7)
  731. PCR primer with no extra DNA end - (reply: 5)
  732. PCR colonies of Pichia pastoris - (reply: 1)
  733. ROX reference dye for quantitative RT-PCR - (reply: 1)
  734. PCR problem - (reply: 9)
  735. why PCR product smeared? - (reply: 2)
  736. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  737. silincing box, no amplification - (reply: 3)
  738. Comparative semiquantitative RT-PCR - (reply: 2)
  739. Bands in my PCR Controls - (reply: 3)
  740. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  741. Strange band in colony PCR - (reply: 2)
  742. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  743. Multiplex PCR - (reply: 12)
  744. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  745. PYO pcr stopped working. - (reply: 1)
  746. Bands seen with qPCR missing in regular PCR - (reply: 5)
  747. checking msp primers - (reply: 2)
  748. PCR product one day, none the next day - (reply: 4)
  749. PCR on colony - (reply: 10)
  750. PCR reaction ISSUES???? need help - (reply: 4)
  751. PCR using CDNA as template - (reply: 2)
  752. RT-PCR doesn't work with all RNA used - (reply: 1)
  753. Need help amplifying repeating sequence. - (reply: 5)
  754. Real Time PCR 101...help! - (reply: 1)
  755. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  756. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  757. Another primer dimers problem - (reply: 23)
  758. Colony PCR Problem!!!! - (reply: 5)
  759. using PCR product as standard template - (reply: 3)
  760. PCR kit - (reply: 7)
  761. Make additional cycles with an already finished pcr product - (reply: 3)
  762. Amplification in water control but not in samples - (reply: 3)
  763. PCR products form with DNA, but not with c-DNA - (reply: 2)
  764. question about pcr amplification efficiency? - (reply: 1)
  765. DNA polymerase: recombinant or native? - (reply: 3)
  766. pcr clean-up fail? - (reply: 1)
  767. MSP U primers very difficult to amplify... - (reply: 4)
  768. No gene amplification, 18s amplification is fine - (reply: 1)
  769. ChIP-qPCR gives odd amplification plot - (reply: 5)
  770. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  771. sequential cloning of multiple PCR products - how to do? (reply: 3)
  772. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  773. Primer design - (reply: 3)
  774. Tomato Actin PCR+Images included - (reply: 2)
  775. single primer PCR - (reply: 5)
  776. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  777. cDNA synthesis + amplification - (reply: 2)
  778. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  779. LacZ PCR Problems - (reply: 1)
  780. cells stably expressing T7 RNA polymerase - (reply: 1)
  781. Primer concentration - (reply: 4)
  782. nested PCR with low target DNA? - (reply: 2)
  783. RT-PCR calibrator - (reply: 2)
  784. RNA - extraction and pcr (reply: 1)
  785. problem in pcr - (reply: 2)
  786. Can someone recommend me a virtual PCR software? - (reply: 6)
  787. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  788. Primer 3' mismatch - Strange experience (reply: 9)
  789. real-time PCR after restriction enzyme digestion - (reply: 6)
  790. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  791. Adding loading/tracking dye to PCR mix? - (reply: 10)
  792. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  793. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  794. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  795. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  796. how to get rid of non specific bands in PCR - (reply: 1)
  797. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  798. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  799. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  800. PCR cloning problem - could not get colony (reply: 4)
  801. pcr contamination - (reply: 4)
  802. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  803. Large Non-specfic bands in PCR - (reply: 3)
  804. PCR clonning - (reply: 3)
  805. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  806. 2 bands in blue colony PCR - (reply: 5)
  807. Primer dilution --> problem.. - (reply: 16)
  808. Help! Smear in Real-Time PCR Product - (reply: 1)
  809. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  810. Sybr green RT-qPCR primer - (reply: 1)
  811. Sybr green RT-qPCR primer - (reply: 1)
  812. primer design - microalgae (reply: 2)
  813. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  814. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  815. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  816. PCR yield in ng/ul - (reply: 1)
  817. No Amplification in PCR - (reply: 4)
  818. Real time PCR and percentage loss - (reply: 1)
  819. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  820. Problem with smear in PCR with certain templates - (reply: 4)
  821. Real time PCR in the presence of heme - (reply: 3)
  822. issues in PCR amplification - (reply: 2)
  823. Problems with PCR in general - (reply: 1)
  824. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  825. how to overcome primer contamination - need protocol (reply: 11)
  826. RT-PCR primer design - Primer design and evaluation (reply: 7)
  827. CHIP analysis by PCR - (reply: 4)
  828. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  829. Primer,probes dilution - (reply: 3)
  830. Splice varient quantification in RT real time PCR? - (reply: 1)
  831. negative control for msp pcr - (reply: 2)
  832. Calculating geometric mean for real-time PCR - (reply: 5)
  833. template transfer? - how does the rna polymerase switches template? (reply: 1)
  834. PCR Gel nonspecific band - (reply: 13)
  835. semiquantitative pcr - (reply: 1)
  836. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  837. PCR: Cloning Primers - (reply: 8)
  838. PCR product - (reply: 5)
  839. pcr amplify an insert in plasmid? - (reply: 2)
  840. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  841. Exon Spanning PCR Primers - (reply: 1)
  842. Colony PCR - (reply: 1)
  843. PCR to confirm double crossover - (reply: 1)
  844. Primer sequences - (reply: 1)
  845. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  846. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  847. PCR Primers - (reply: 2)
  848. Cleaning up RT-PCR product before sequencing - (reply: 1)
  849. Template Amount for PCR Amplification - (reply: 6)
  850. How to blast methylation specific and unspecific primers - (reply: 1)
  851. RT-PCR cDNA synthesis - (reply: 6)
  852. problems amplifying from cDNA - (reply: 4)
  853. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  854. No DNA after PCR purification with QiaQuick? - (reply: 4)
  855. restriction digestion against colony PCR - (reply: 1)
  856. How to check primers are of correct sequence or not? - (reply: 2)
  857. Primers... - (reply: 1)
  858. When are primer dimers a problem? - (reply: 1)
  859. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  860. PCR: always got band in the negative control - (reply: 8)
  861. primer with higher melting temperature - (reply: 2)
  862. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  863. Problems designing primers for BSP - (reply: 2)
  864. qPCR primer design - possible off-target (reply: 1)
  865. Primer dimer formation and real-time PCR - (reply: 7)
  866. Identify PCR products - (reply: 3)
  867. PCR genomic DNA using cell lysate - (reply: 1)
  868. Maximum size of overhangs in PCR - (reply: 6)
  869. Colony PCR doesn't work anymore - (reply: 5)
  870. RT-PCR t-test, and ANOVA - (reply: 1)
  871. Primer Design Urgent! - (reply: 1)
  872. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  873. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  874. PCR of microRNA first strand cDNA - (reply: 1)
  875. how to know my primer's sense ??? - help ! (reply: 2)
  876. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  877. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  878. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  879. intron spanning primers for non model organisms - (reply: 2)
  880. Primer design - (reply: 2)
  881. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  882. qPCR amplification interference fixed with freezing - (reply: 6)
  883. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  884. software for quantifing PCR product band - (reply: 1)
  885. Multiple bands from purified PCR product - (reply: 3)
  886. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  887. Doing PCR on Nebulized DNA - (reply: 2)
  888. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  889. RT-PCR - (reply: 9)
  890. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  891. inverse pcr - (reply: 2)
  892. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  893. Primer seq - (reply: 2)
  894. Urgent: should you dry PCR product - (reply: 13)
  895. PCR product size - (reply: 6)
  896. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  897. oligo dt and random primer - (reply: 3)
  898. mRNA Search for RT-PCR (U to T) - (reply: 2)
  899. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  900. PCR Mastermix - (reply: 4)
  901. PCR triplicates versus one reaction - differences? (reply: 17)
  902. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  903. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  904. Primers and self and hetero dimers - (reply: 1)
  905. Really weird amplification curves - (reply: 1)
  906. Primers for qPCR - (reply: 3)
  907. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  908. RT-PCR primer - (reply: 3)
  909. PCR problem - basic PCR for plasmid amplification (reply: 2)
  910. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  911. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  912. Unpredictablity of PCR product - (reply: 5)
  913. total cDNA amplification by PCR - (reply: 2)
  914. Detection limit of a conventional PCR - (reply: 2)
  915. PCR for MULTIPLE mutagenesis - (reply: 4)
  916. Using Sybr green in realtime pcr - (reply: 1)
  917. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  918. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  919. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  920. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  921. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  922. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  923. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  924. Digested PCR product migrate slower than uncut - (reply: 8)
  925. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  926. PCR question - (reply: 2)
  927. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  928. 2 rounds PCR got problem - (reply: 2)
  929. PCR help minus strand - Primer design (reply: 1)
  930. chloramphenicol storage and amplification - (reply: 3)
  931. Smear in long distance PCR - (reply: 39)
  932. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  933. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  934. TOUCH DOWN PCR - (reply: 2)
  935. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  936. PCR stopped working - After changing buffer (reply: 5)
  937. ChIP PCR question - (reply: 33)
  938. PCR protocol questions! - (reply: 2)
  939. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  940. DNA amount calculation for PCR - (reply: 14)
  941. ChIP primer design - (reply: 1)
  942. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  943. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  944. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  945. How to analyze ChIP PCR data - (reply: 10)
  946. Questions regarding RT-PCR optimization - (reply: 2)
  947. number of copies after pcr? - (reply: 1)
  948. PCR double-bands - (reply: 1)
  949. Problems with semi-quatitative PCR - (reply: 1)
  950. Question about Primers - (reply: 3)
  951. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  952. Large fragment amplification failed - (reply: 3)
  953. purification of PCR product for cloning in a vector - (reply: 3)
  954. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  955. PCR reaction calculation - (reply: 2)
  956. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  957. Primer design with a tag - (reply: 4)
  958. no increase in fluorescence in my real time PCR - (reply: 3)
  959. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  960. Help with real-time PCR quantification of miRNA - (reply: 8)
  961. PCR, using gel extracted band as a template - (reply: 4)
  962. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  963. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  964. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  965. Combining forward and reverse primer gives different size on gel - (reply: 2)
  966. Q-PCR and RT-PCR - (reply: 3)
  967. calculating total amount - in a pcr reaction (reply: 4)
  968. Problems with amplifying microRNA - (reply: 3)
  969. PCR of complement genomic DNA - (reply: 3)
  970. primers for fungal identification - (reply: 9)
  971. confusing PCR - (reply: 1)
  972. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  973. PCR for long and repetitive region from genomic DNA - (reply: 4)
  974. difference between PCR primers and sequencing primers - (reply: 1)
  975. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  976. PCR of repeated region - (reply: 1)
  977. Sense and antisense DNA, and primer design - (reply: 6)
  978. PCR bands in NTC but NOT in negative samples - (reply: 2)
  979. housekeeping genes in PCR - (reply: 1)
  980. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  981. Question about RealTime PCR Primer Design - (reply: 1)
  982. Designing a Primer with a RS that has a W - (reply: 1)
  983. Primers stopped working!? - PCR Primers (reply: 6)
  984. NFK beta primers - (reply: 4)
  985. final PCR product - need clarifications (reply: 1)
  986. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  987. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  988. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  989. RT-PCR result !! - (reply: 4)
  990. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  991. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  992. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  993. why genomic can't be use for pcr? - (reply: 2)
  994. Primer design - Primer design (reply: 2)
  995. Re-use unamplified PCR product - (reply: 2)
  996. Reason for odd PCR conditions - (reply: 3)
  997. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  998. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  999. pcr - problem in pcr (reply: 2)
  1000. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  1001. How to present and analyze these real-time PCR data? - (reply: 2)
  1002. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  1003. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  1004. PCR Master mix - (reply: 2)
  1005. cDNA storage and real time RT PCR - (reply: 3)
  1006. Real Time PCR Primers and Probes - (reply: 2)
  1007. Anyone make their own PCR cloning vector? - (reply: 5)
  1008. Stability of PCR T-overhangs - (reply: 1)
  1009. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  1010. PCR product wrong size! Need help! - (reply: 9)
  1011. pooling different pcr cycles? - (reply: 1)
  1012. Amplification of concatenated linear ligated fragments - (reply: 4)
  1013. Clinical Real-time PCR assay - (reply: 1)
  1014. Need help - How we can design primer for miRNA (reply: 1)
  1015. primer and promoter sequences - (reply: 1)
  1016. Standard curve for real-time PCR - (reply: 2)
  1017. designing of primers - (reply: 2)
  1018. Primer Tm differences/ PCR - (reply: 1)
  1019. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  1020. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  1021. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  1022. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  1023. Designing primer to remove his-tag - (reply: 1)
  1024. PCR Primer Dilution from F+R - (reply: 1)
  1025. primer reconstitution - primer reconstitution (reply: 4)
  1026. Not-Quite-Nested PCR - (reply: 2)
  1027. PCR product too short - (reply: 6)
  1028. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  1029. Primer design for insert amplification - Is this correct? (reply: 2)
  1030. got smear on the PCR gel !!! - (reply: 4)
  1031. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  1032. "Unzipped" PCR band - (reply: 3)
  1033. Review on new amplification techniques - (reply: 2)
  1034. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  1035. DOT BLOT for pcr product - No result obtain (reply: 1)
  1036. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  1037. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  1038. HRM primers - (reply: 1)
  1039. PCR amplification with Pfu / quality of DNA - (reply: 4)
  1040. primer design - (reply: 2)
  1041. Is it possible to compare data between real-time PCR plates - (reply: 1)
  1042. Conventional PCR problems - wisamni2004 (reply: 2)
  1043. New to sequencing, primer design - (reply: 2)
  1044. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  1045. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  1046. incorrect/smaller band size for real time PCR - (reply: 1)
  1047. Quickchange mutagenesis primer - (reply: 3)
  1048. pcr / primer theory in bisulfite sequencing - (reply: 4)
  1049. homologous amplicon for real time pcr - (reply: 1)
  1050. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  1051. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  1052. assistance with PCR amplification please - (reply: 3)
  1053. I need an urgent help with q-pcr amplification plots - (reply: 11)
  1054. Problems with the Specificity of the Primers - (reply: 2)
  1055. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  1056. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  1057. Degenerate primers PCR problem, Please help! - (reply: 2)
  1058. Hotstart PCR and unspecific Amplification - (reply: 3)
  1059. Primer design help needed - (reply: 1)
  1060. Primers no longer work - (reply: 1)
  1061. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  1062. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  1063. RT PCR for known snps detection? - SNPs detection (reply: 1)
  1064. colony PCR - (reply: 2)
  1065. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  1066. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  1067. PCR reaction without template gives a product - (reply: 6)
  1068. PCR wrong product size - (reply: 6)
  1069. Wrong pcr product size - (reply: 4)
  1070. real time PCR machine - (reply: 5)
  1071. PCR very faint product - (reply: 1)
  1072. Question about principle of PCR - (reply: 3)
  1073. PCR amplification before bisulfite conversion - (reply: 6)
  1074. 4 question for real time PCR - (reply: 5)
  1075. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  1076. PCR didn't work - suspect the enzyme (reply: 2)
  1077. Find universal primers in vector - any online tool? (reply: 6)
  1078. Picking primers to confirm Illumina meth27 results - (reply: 2)
  1079. Colony PCR Specificity - (reply: 2)
  1080. Colony Pcr - primers - (reply: 5)
  1081. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  1082. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  1083. Primer Dilution Problem - D'oh! (reply: 2)
  1084. Using PCR to create overhangs - (reply: 3)
  1085. Creating overhangs with PCR - (reply: 4)
  1086. Problem with PCR on bacmids - (reply: 1)
  1087. Is there any software that can be sued to design degenerate primers - (reply: 1)
  1088. Running real time pcr product on gel?.. - (reply: 4)
  1089. Primer Decontamination - (reply: 2)
  1090. PCR mutagenesis of plasmid - (reply: 5)
  1091. tools for checking non-specific binding of primers -
    (reply: 1)
  1092. Primer designed for ARMS PCR - (reply: 2)
  1093. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  1094. methylation specific PCR - Primers - (reply: 1)
  1095. bacterial identification using real time PCR - (reply: 1)
  1096. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1097. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1098. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1099. colony PCR for subcloning gene - (reply: 1)
  1100. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1101. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1102. PCR reamplification - (reply: 2)
  1103. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1104. How many colonies to screen (colony PCR)? - (reply: 4)
  1105. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1106. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1107. question about pcr - (reply: 3)
  1108. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1109. How to construct a standard curve for real time PCR - (reply: 1)
  1110. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1111. PCR with one primer - (reply: 1)
  1112. colony PCR inconsistent - (reply: 4)
  1113. PCR contamination - (reply: 19)
  1114. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1115. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1116. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1117. PCR not working - No amplification - (reply: 5)
  1118. Quantification of PCR products - (reply: 1)
  1119. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1120. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1121. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1122. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1123. Nested PCR question - (reply: 1)
  1124. High Tm on primers - cannot get a product - (reply: 8)
  1125. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1126. undesired products in multiplex PCR - (reply: 3)
  1127. NCBI primer blast problem - (reply: 1)
  1128. Help with Multiplex Nested PCR - (reply: 2)
  1129. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1130. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1131. primer sequence problem - (reply: 2)
  1132. Finding bisulfite PCR primer sequence - (reply: 3)
  1133. PCR -No band formation - (reply: 1)
  1134. PCR - No Band formation - (reply: 3)
  1135. No amplification after bisulfite treatment - (reply: 4)
  1136. PCR AMPLIFICATION - (reply: 1)
  1137. Ligation of Blunt PCR Product - (reply: 1)
  1138. real time pcr need advise - (reply: 2)
  1139. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1140. PCR & Cloning - (reply: 6)
  1141. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1142. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1143. DNA pooling for PCR - Saving money PCR (reply: 7)
  1144. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1145. PCR need some help - (reply: 4)
  1146. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1147. Left polymerase out overnight - Will it still work? (reply: 1)
  1148. Primer Reconstitution--does temperature matter? - (reply: 5)
  1149. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1150. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1151. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1152. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1153. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1154. no PCR product from input... - (reply: 8)
  1155. MSP - unwanted amplification - (reply: 5)
  1156. Smears in Bisulfite seq. PCR - (reply: 3)
  1157. Primer design for qPCR - (reply: 2)
  1158. Primer design for qPCR - (reply: 1)
  1159. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1160. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1161. Multiple bands after bisulfite PCR - (reply: 7)
  1162. PCR genomic DNA of high GC content - (reply: 6)
  1163. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1164. primer design tips - (reply: 3)
  1165. PCR with Biotin Incorporation - (reply: 2)
  1166. pcr product - hello all (reply: 1)
  1167. design pcr primer - (reply: 1)
  1168. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1169. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1170. PCR with long and complex primers - (reply: 2)
  1171. Phospholylation of primers - (reply: 1)
  1172. apoptosis by PCR? - (reply: 12)
  1173. Re-using PCR plates? - (reply: 2)
  1174. Touchdown PCR issues - (reply: 3)
  1175. LINE1 OFR2 Primer Design - (reply: 1)
  1176. phosphorylation of primers using PNK - (reply: 1)
  1177. RT-PCR contamination issue - (reply: 3)
  1178. Primers for yeast (18S) - (reply: 1)
  1179. How to reduce the bisulfite PCR bias? - (reply: 4)
  1180. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1181. Primer Concentration to lower Ct - (reply: 1)
  1182. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1183. Primer Concentration Help - (reply: 4)
  1184. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1185. Primer Degradation - What exactly happens? (reply: 1)
  1186. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1187. PCR/DNA Extraction Problem - (reply: 5)
  1188. Primers in 2 exons - (reply: 2)
  1189. NTC appear in real time pcr - (reply: 6)
  1190. Problems with designing a primer - (reply: 2)
  1191. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1192. PCR of AT-rich DNA - (reply: 1)
  1193. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1194. Overlapping PCR - really need help ! - (reply: 5)
  1195. Freezing PCR product??? - (reply: 1)
  1196. problem with pcr cloning from mouse cDNA - (reply: 2)
  1197. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1198. PCR with Platinum Taq - product yield issues - (reply: 3)
  1199. real time PCR - (reply: 7)
  1200. Different sized product for same BS primer! - (reply: 2)
  1201. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1202. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1203. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1204. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1205. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1206. primers with restriction site - (reply: 1)
  1207. PCR product appear two close band in my gel - (reply: 15)
  1208. The Case of a Missing Band: PCR Issue - (reply: 4)
  1209. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1210. cDNA as PCR template - (reply: 1)
  1211. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1212. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1213. paranormal qpcr amplification activity - melting curves (reply: 3)
  1214. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1215. PCR template concentration - (reply: 6)
  1216. Bands in PCR negative control - (reply: 3)
  1217. PCR efficiency calculated by Linreg - (reply: 3)
  1218. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1219. primer design by generunner DG - (reply: 1)
  1220. degenerate pcr - (reply: 1)
  1221. BSP primers design, help please - (reply: 2)
  1222. primers with fluoresent dye PCR cycle question - (reply: 4)
  1223. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1224. Smears on PCR products - (reply: 4)
  1225. Dumb Reverse Transcription PCR Question - (reply: 2)
  1226. Reverse transcription PCR - (reply: 1)
  1227. PCR/RT-PCR beads - (reply: 2)
  1228. High Amplification Efficiency in Std. Curve - (reply: 5)
  1229. Non specific products in Bisulphite pcr - (reply: 4)
  1230. Run PCR amplifications in agarose gels - (reply: 3)
  1231. Problems with validating primers and low expression genes - (reply: 3)
  1232. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1233. Bisulfite sequencing PCR not working - (reply: 5)
  1234. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1235. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1236. intron/exon spanning primers - (reply: 1)
  1237. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1238. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1239. PCR product for sequencing - sample storage?? (reply: 2)
  1240. Absolute quantification using Real Time PCR - (reply: 2)
  1241. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1242. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1243. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1244. MSP primer troubles - (reply: 3)
  1245. Measuring global methylation using real time PCR - (reply: 2)
  1246. Designing Primers for multiple Isoforms - (reply: 1)
  1247. RT-PCR standard curve dilutions - (reply: 2)
  1248. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1249. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1250. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1251. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1252. PCR product dimer issue - (reply: 24)
  1253. PCR additives Formamide - Formamide which one? (reply: 4)
  1254. PCR Master mix - (reply: 1)
  1255. Addition of Restriction sites into PCR primers - (reply: 4)
  1256. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1257. failure PCR amplification from low GC content gene - (reply: 5)
  1258. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1259. conventional v real-time PCR applications - (reply: 1)
  1260. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1261. PCR reagents, can I use reagents from different manufacturers? - (reply: 6)
  1262. different PCR primers for real-time and classic PCR - (reply: 3)
  1263. primers deconamination??? - (reply: 6)
  1264. PCR band too thick - PCR troubleshooting question (reply: 5)
  1265. RT-PCR problem - (reply: 1)
  1266. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1267. primer check!!! - (reply: 6)
  1268. PCR bands on gel electrophoresis - (reply: 5)
  1269. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1270. long range PCR - (reply: 2)
  1271. Annealing Temperature of biotinylated primers - (reply: 2)
  1272. RT-PCR - New to RT-PCR info (reply: 2)
  1273. PCR punch - (reply: 4)
  1274. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1275. PCR off plasmid for screening - (reply: 2)
  1276. PCR failed no product.. help - (reply: 10)
  1277. number of molecules in PCR - (reply: 3)
  1278. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1279. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1280. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1281. Primer dilution - PCR primer dilution (reply: 2)
  1282. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1283. Can't re-PCR the PCR product - (reply: 5)
  1284. PCR reagent autoclave - (reply: 2)
  1285. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1286. Long Primers for PCR - (reply: 2)
  1287. Data Analysis for Real-time PCR - (reply: 2)
  1288. PCR not working - overhangs too long? (reply: 3)
  1289. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1290. argh more pcr headaches! - (reply: 6)
  1291. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1292. Chlamydia PCR cycling conditions - (reply: 1)
  1293. Primers going "loopy" - (reply: 2)
  1294. Conversion of primer unit - ug/ul to uM (reply: 4)
  1295. mutagenesis by PCR or just use a kit - (reply: 5)
  1296. Unsuccessful BS PCR - (reply: 3)
  1297. Primer Trouble Shooting - (reply: 5)
  1298. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1299. Smearing on gel in PCR products - (reply: 5)
  1300. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1301. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1302. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1303. Relative RT-PCR - (reply: 2)
  1304. Relative real time RT PCR - (reply: 2)
  1305. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1306. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1307. a simple primer problem - (reply: 5)
  1308. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1309. no or poor amplification - (reply: 1)
  1310. Primers from 3'UTR - (reply: 2)
  1311. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1312. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1313. Designing primers with ESTs - (reply: 2)
  1314. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1315. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1316. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1317. Real Time vs Traditional PCR results - (reply: 1)
  1318. The problems to identify mouse genotype with PCR - (reply: 1)
  1319. addition of BclI restriction site to PCR primer - (reply: 6)
  1320. non specific amplification - DNA amplification by PCR (reply: 1)
  1321. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1322. How does methprimer calculate primer Tm - (reply: 2)
  1323. Laminar Flow vs PCR cabinet - (reply: 4)
  1324. How to do PCR to detect mRNA without RT? - (reply: 2)
  1325. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1326. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1327. PCR has stopped working - (reply: 7)
  1328. PCR negative control contamination - (reply: 8)
  1329. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1330. Phosphorylated primers - (reply: 5)
  1331. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1332. PCR efficiency in real timePCR - (reply: 5)
  1333. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1334. Drastic Decrease in PCR product yield - (reply: 2)
  1335. resources on PCR principles & technique - (reply: 1)
  1336. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1337. Colony Screeing without using PCR - (reply: 8)
  1338. High MW PCR band seen...help needed - (reply: 7)
  1339. QPCR - Primer and Probe question - QPCR (reply: 2)
  1340. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1341. standard curve/ PCR efficiency problems... - (reply: 1)
  1342. Help! PCR that used to work doesn't work now! - (reply: 2)
  1343. PCR and templates - (reply: 3)
  1344. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1345. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1346. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1347. Easy primer question? - Primers (reply: 1)
  1348. PCR inconsistency - (reply: 4)
  1349. Plasmid problem - From PCR product (reply: 7)
  1350. help needed: PCR a gene from genomic DNA - (reply: 2)
  1351. questions about pcr products after pooling - (reply: 3)
  1352. stiching/linking/sewing pcr - (reply: 3)
  1353. Human mtDNA amplification problems - (reply: 3)
  1354. primer dimer - proplems (reply: 1)
  1355. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1356. Nested PCR - (reply: 2)
  1357. pfu vs long pcr mix - (reply: 7)
  1358. weird PCR ask for help - (reply: 12)
  1359. Methylation specific DMSO PCR - (reply: 1)
  1360. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1361. No PCR product at all - (reply: 8)
  1362. PCR troubleshoooooting - primer dimer (reply: 1)
  1363. Primer efficiency test - (reply: 1)
  1364. primer software - (reply: 2)
  1365. Question on wired PCR - (reply: 1)
  1366. restriction digestion of PCR product - (reply: 7)
  1367. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1368. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1369. How to know in which exon a primer match? - (reply: 1)
  1370. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1371. Amplification of human genomic DNA - (reply: 2)
  1372. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1373. sequencing with forward/reverse primers - (reply: 7)
  1374. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1375. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1376. Adding A overhangs - primer design implications? - (reply: 1)
  1377. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1378. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1379. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1380. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1381. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1382. primer design problem - (reply: 1)
  1383. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1384. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1385. Problem with primer efficiency analysis - (reply: 4)
  1386. slan real time PCR system for validation of microarray results? - (reply: 1)
  1387. PCR unusualband at ~230bps - PCR (reply: 1)
  1388. DMSO or BSA for PCR - (reply: 6)
  1389. Whole Genome Amplification - (reply: 2)
  1390. primer design - (reply: 1)
  1391. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1392. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1393. designing primers for genes not sequenced yet - (reply: 2)
  1394. Tris Buffer for PCR reaction - why? (reply: 1)
  1395. PCR a plasmid protein - (reply: 2)
  1396. multiplex PCR - (reply: 4)
  1397. Realtime PCR machine - (reply: 2)
  1398. PCR set-up calculation nightmares. - (reply: 3)
  1399. Primer desing - (reply: 2)
  1400. Colony PCR - (reply: 3)
  1401. Standard curves for PCR efficiency. - (reply: 2)
  1402. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1403. Question about RAPD PCR - (reply: 2)
  1404. Looking for help with my PCR! - (reply: 3)
  1405. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1406. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1407. primer design - (reply: 9)
  1408. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1409. PCR without thermal cycler? - (reply: 9)
  1410. primer contamination - primer contaminated with ice (reply: 3)
  1411. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1412. RT-PCR problem - (reply: 4)
  1413. Only DNA ladder , No desired band in PCR - (reply: 4)
  1414. taq and PCR - (reply: 6)
  1415. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1416. PCR Efficiency - (reply: 2)
  1417. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1418. Long PCR and genomic DNA isolation problems - (reply: 2)
  1419. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1420. NTC with specific amplification - (reply: 1)
  1421. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1422. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1423. Primer3 vs Primer BLAST - (reply: 3)
  1424. PCR and then ligation - (reply: 7)
  1425. Primer design: free energy - (reply: 2)
  1426. Amplification in NTC and noRT controls - (reply: 5)
  1427. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1428. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1429. asymmetric PCR - (reply: 1)
  1430. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1431. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1432. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1433. trouble with pcr - (reply: 8)
  1434. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1435. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1436. Real time PCR results-interpretation - (reply: 1)
  1437. Real time PCR results-interpretation - (reply: 2)
  1438. Primer optimization for ChIP - (reply: 2)
  1439. sequencing primer: T7 or T7promoter? - (reply: 4)
  1440. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1441. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1442. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1443. primer tm - tm calculation for tagged primers (reply: 2)
  1444. Reusing 96 well plates for PCR - (reply: 2)
  1445. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1446. PCR and AFLP - (reply: 1)
  1447. Universal 16s rRNA primers needed - (reply: 3)
  1448. Identifying PCR Inhibitors - (reply: 10)
  1449. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1450. PCR + phusion enzyme = massive errors - (reply: 4)
  1451. long DNA amplification - (reply: 11)
  1452. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1453. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1454. adding koazak sequence to primer - (reply: 1)
  1455. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1456. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1457. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1458. Primer design if sequence is unknown for organism - (reply: 5)
  1459. adding C terminal tag to reverse primer - (reply: 1)
  1460. epitope tagging of pcr products - (reply: 1)
  1461. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1462. Real time PCR info - (reply: 4)
  1463. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1464. PCR from BAC - PCR from BAC (reply: 1)
  1465. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1466. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1467. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1468. How much product PCR?? - (reply: 3)
  1469. what primers should I use for DNA sequencing? please help - (reply: 2)
  1470. what primers should I use for DNA sequencing?? - (reply: 3)
  1471. Is my primer going to work for qRT-PCR? - (reply: 6)
  1472. Standard curve for RT-PCR - (reply: 1)
  1473. PCR using genomic DNA - (reply: 2)
  1474. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1475. Problem amplifying plasmid - (reply: 1)
  1476. Sybr Green I in real-time PCR reaction - (reply: 3)
  1477. Primers annealing temperatures - (reply: 8)
  1478. Difficulties cloning by PCR - (reply: 5)
  1479. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1480. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1481. high Cp value PCR - (reply: 2)
  1482. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1483. Synthesize own Oligo-dT primers - (reply: 6)
  1484. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1485. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1486. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1487. Smearing/ no amplification in PCR - (reply: 3)
  1488. primer design - Primer design for PCR (reply: 1)
  1489. How are primers designed? - (reply: 4)
  1490. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1491. DNA methylated PCR - (reply: 2)
  1492. need help on primer calcuation soon - (reply: 1)
  1493. primer design - (reply: 6)
  1494. problem in pcr and purification - (reply: 3)
  1495. Inability to duplicate PCR results - (reply: 8)
  1496. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1497. PCR using xt5 - (reply: 2)
  1498. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1499. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1500. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1501. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1502. 23S rRNA Primer - need primer sequence (reply: 1)
  1503. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1504. RT-PCR - problems with TaqMan PCR (reply: 2)
  1505. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1506. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1507. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1508. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1509. Small product amplification problems - (reply: 4)
  1510. multi way ligation or long range pcr? - (reply: 1)
  1511. Freezing primers+SYBR green - (reply: 2)
  1512. Tagged Primers - (reply: 5)
  1513. no pcr amplification product - (reply: 9)
  1514. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1515. design primer for chip - (reply: 5)
  1516. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1517. PCR problem - (reply: 4)
  1518. differently behavioring replicates in real-time PCR - (reply: 1)
  1519. Multiplex PCR - (reply: 1)
  1520. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1521. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1522. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1523. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1524. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1525. PCR components storage - (reply: 3)
  1526. reconstitution of primers - (reply: 2)
  1527. No PCR bands with some templates - (reply: 5)
  1528. Mysterious PCR Contamination - (reply: 13)
  1529. BS PCR..please help - DNA methylation analysis (reply: 15)
  1530. RT-PCR Help - Protocol provided (reply: 4)
  1531. PCR product purification - (reply: 3)
  1532. Mutagenesis PCR and undesired amplification - (reply: 2)
  1533. Temp Gradient PCR - (reply: 2)
  1534. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1535. PCR with genomic dna - (reply: 9)
  1536. primer dimer - (reply: 2)
  1537. PCR with long primers - (reply: 3)
  1538. BSP PCR standardization...help - (reply: 8)
  1539. PCR on degraded templates - (reply: 2)
  1540. PCR help needed - (reply: 3)
  1541. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1542. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1543. light bands, dark smear, dark dimers - (reply: 2)
  1544. PCR, followed by sequencing... - why ?? (reply: 3)
  1545. PCR program - strange extension step (reply: 2)
  1546. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1547. Help me! How to design nested BSP primers? - (reply: 4)
  1548. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1549. Plasmid linearization by PCR - (reply: 4)
  1550. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1551. Using BLAST to check primers - (reply: 17)
  1552. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1553. pcr doesnt show any band for DNa walking - (reply: 8)
  1554. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1555. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1556. Effects of more cDNA in PCR??? - (reply: 1)
  1557. PCR primer - (reply: 2)
  1558. PCR product disappears after restriction digestion - (reply: 4)
  1559. Primer design - GC clamp - (reply: 8)
  1560. Real Time PCR - excluding Ct - (reply: 2)
  1561. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1562. Price of a real time PCR machine? - (reply: 4)
  1563. colony PCR - (reply: 8)
  1564. probe conc. of Asymmetric PCR - (reply: 1)
  1565. primer dimer or what? - (reply: 2)
  1566. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1567. Real Time PCR method comments - (reply: 2)
  1568. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1569. colony pcr - (reply: 4)
  1570. Primers and Taqman Probes mixture question - (reply: 1)
  1571. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1572. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1573. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1574. Colony PCR - (reply: 15)
  1575. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1576. no bands in my PCR - (reply: 9)
  1577. designing primers - (reply: 1)
  1578. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1579. purefication and amplification of serum free DNA - (reply: 1)
  1580. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1581. Bisulfite Sequencing PCR - (reply: 4)
  1582. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1583. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1584. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1585. mRNA PCR - (reply: 1)
  1586. pcr product quantification - (reply: 5)
  1587. Negative Control Primers for ChIP Assay - (reply: 3)
  1588. Magnesium in PCR - (reply: 1)
  1589. PCR Sample Prep - (reply: 4)
  1590. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1591. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1592. Bizarre Contamination in PCR - (reply: 7)
  1593. PCR Cloning-large primers - (reply: 4)
  1594. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1595. forward and reverse primer - (reply: 4)
  1596. Check the primers - (reply: 4)
  1597. PCR problem from transformed TOPO TA vector - (reply: 3)
  1598. DIG DNA PCR labeling problem - (reply: 6)
  1599. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1600. BSP primers - (reply: 2)
  1601. Various size of insert after colony screen by PCR - (reply: 2)
  1602. inverse PCR molecular bilogy - (reply: 3)
  1603. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1604. failed PCR on DNA extract from blood - (reply: 6)
  1605. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1606. Primer dimer issue in real time PCR - (reply: 21)
  1607. Leaky RT-PCR - (reply: 4)
  1608. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1609. PCR, RNA, Northern Blotting???? - (reply: 1)
  1610. bizzare PCR smear - why does science hate me (reply: 7)
  1611. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1612. PCR detection of SNP - (reply: 6)
  1613. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1614. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1615. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1616. Sub-cloning sticky-end PCR products - (reply: 2)
  1617. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1618. ChIP on chip amplification problems - (reply: 11)
  1619. Genotyping PCR - (reply: 3)
  1620. PCR screening of transformed bacterial colony - (reply: 6)
  1621. Is this standard valid? - pcr product as standards (reply: 5)
  1622. primer binding - (reply: 1)
  1623. weird ChIP primers! - (reply: 1)
  1624. PCR primers - (reply: 1)
  1625. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1626. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1627. amplification in negative control in RT PCR - (reply: 2)
  1628. miRNA real time PCR by SYBR green methods - (reply: 1)
  1629. Deletion PCR - (reply: 9)
  1630. diluting to final primer concentration - please help correct (reply: 1)
  1631. Smear problem with my new primer set - (reply: 2)
  1632. PCR ghost bands - (reply: 1)
  1633. Amplifying a gene using a degenerate primer - (reply: 4)
  1634. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1635. CP of real time PCR by LC480 - (reply: 1)
  1636. CHIP- Real Time PCR calculations - (reply: 1)
  1637. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1638. transgene copy number - using real time PCR (reply: 1)
  1639. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1640. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1641. Unspecific PCR - (reply: 7)
  1642. Real-Time PCR as a Microplate Reader - (reply: 1)
  1643. gene-specific RT-PCR - (reply: 4)
  1644. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1645. RT-PCR Internal Standard - (reply: 2)
  1646. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1647. Plasmid supercoiling affecting PCR? - (reply: 2)
  1648. RNA contamination in PCR - (reply: 1)
  1649. PCR without DNA extraction! - (reply: 2)
  1650. Primer tm - (reply: 1)
  1651. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1652. re-amplification in real-time PCR - (reply: 1)
  1653. primer design - really necessary? (reply: 2)
  1654. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1655. RT-PCR - (reply: 3)
  1656. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1657. Degenerative primers = multiple products? - (reply: 5)
  1658. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1659. PCR problems on a ligation product - (reply: 5)
  1660. Genomic DNA and PCR - (reply: 4)
  1661. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1662. Sewing PCR - (reply: 3)
  1663. on the minimal pcr derived product - (reply: 2)
  1664. primer dimers - (reply: 5)
  1665. RT-PCR vs. conventional PCR - (reply: 1)
  1666. qPCR primer design - (reply: 5)
  1667. PCR and gel purification problem - did I screw this up (reply: 4)
  1668. Cloning PCR fragment - (reply: 1)
  1669. touchdown pcr - (reply: 6)
  1670. non specific pcr products - (reply: 4)
  1671. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1672. Anyone with experiences in 2 step PCR - (reply: 1)
  1673. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1674. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1675. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1676. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1677. re-amplification of a PCR product - is it recommended? (reply: 7)
  1678. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1679. PCR machine with accurate temperature control? - (reply: 1)
  1680. Gene-specific RT-PCR - (reply: 2)
  1681. Primer stock confusion - (reply: 6)
  1682. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1683. primer design - primer design question (reply: 2)
  1684. Protocol for 16S rDNA real time PCR - (reply: 2)
  1685. Touchdown PCR - (reply: 1)
  1686. Determine annealing temperature of primers - (reply: 14)
  1687. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1688. polymerase for BSP - (reply: 2)
  1689. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1690. Validating real time pcr primers - (reply: 2)
  1691. BSP,MSP primer design? - (reply: 1)
  1692. low gc primers - help with pcr using low gc primers (reply: 6)
  1693. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1694. Primers and annealing temperature - (reply: 2)
  1695. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1696. Copy number calculations in real time PCR - (reply: 2)
  1697. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1698. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1699. RT-PCR Negative controls - (reply: 1)
  1700. WHich primer to use for sequencing - (reply: 5)
  1701. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1702. minimum length for the gene to be amplified in PCR - (reply: 6)
  1703. dNTP Quantity - (reply: 3)
  1704. annoying PCR cloning problem (season 2) - (reply: 5)
  1705. PCR with 60 bp primers - results in no product (reply: 6)
  1706. Amplification dwindles using little template RT-PCR - (reply: 3)
  1707. Running gel after RT-PCR - (reply: 5)
  1708. Degenerate PCR Size Limit? - (reply: 3)
  1709. primer design for pBAD topo expression kit - (reply: 2)
  1710. PCR problems.. - problems with conventional PCR (reply: 3)
  1711. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1712. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1713. small PCR products on agarose gel? - (reply: 8)
  1714. Dnase digestion and PCR - (reply: 4)
  1715. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1716. primers fpr sequencing - (reply: 4)
  1717. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1718. Need an UV260 RT-PCR instrument - (reply: 1)
  1719. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1720. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1721. PCR produces products of wrong size - (reply: 4)
  1722. PCR with pfu and degenerate primers (?) - (reply: 7)
  1723. a doubt about PCR gel purification - (reply: 4)
  1724. Draw PCR primer locations - PCR primer (reply: 4)
  1725. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1726. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1727. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1728. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1729. PCR+enhancer - (reply: 4)
  1730. Serious issues with RT-PCR - (reply: 7)
  1731. PCR [dNTP] - dNTP concentrations (reply: 2)
  1732. PCR issue - (reply: 2)
  1733. dNTP question - (reply: 3)
  1734. PCR - (reply: 1)
  1735. long PCR primer - (reply: 7)
  1736. Taqman rtPCR primer and probe design - (reply: 3)
  1737. sequencing problem after pcr - (reply: 4)
  1738. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1739. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1740. primer design - (reply: 3)
  1741. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1742. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1743. Analysis of ChIP RT-PCR data - (reply: 7)
  1744. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1745. Negative flouroscence in real time PCR - (reply: 2)
  1746. Minimum number of PCR cycles to see a product? - (reply: 6)
  1747. decontamination - decontamination for PCR (reply: 6)
  1748. Troubleshoot ARMS PCR - (reply: 2)
  1749. negative control for PCR - (reply: 1)
  1750. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1751. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1752. pcr pdt amplification - (reply: 1)
  1753. real time PCR - (reply: 3)
  1754. PCR fails when I scale up - (reply: 2)
  1755. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1756. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1757. PCR not Working = SMEAR - (reply: 3)
  1758. How to do PCR fragment in 2 steps - (reply: 3)
  1759. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1760. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1761. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1762. RT-PCR - problem of PCR (reply: 1)
  1763. PCR product longer than template - why? - (reply: 2)
  1764. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1765. Dye recipe that can be used for PCR MM - (reply: 3)
  1766. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1767. PCR conditions with three primers - (reply: 1)
  1768. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1769. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1770. Cloning a labeled PCR product - (reply: 1)
  1771. PCR and cloning - (reply: 14)
  1772. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1773. PCR product - not suppose to be there (reply: 7)
  1774. using the meth primer - (reply: 2)
  1775. Generating primers for MSP - (reply: 1)
  1776. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1777. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1778. PCR from a smear genomic DNA ? - (reply: 5)
  1779. Primer design and need help - (reply: 11)
  1780. Setting up a multiplex PCR assay. - (reply: 2)
  1781. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1782. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1783. cDNA and RT-PCR - (reply: 8)
  1784. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1785. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1786. PCR problem - help me plsss (reply: 9)
  1787. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1788. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1789. PCR efficiencies - (reply: 4)
  1790. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1791. PCR quantification - (reply: 1)
  1792. PCR quantification - (reply: 1)
  1793. Long PCR product - (reply: 9)
  1794. problem in Pcr amplification - (reply: 7)
  1795. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1796. difference between hotstart and taq polymerase - (reply: 7)
  1797. sequencing the pcr product - (reply: 15)
  1798. Pcr amplification - primer designed from UTR regions (reply: 5)
  1799. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)