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PCR Related Discussions
  1. Diagnostic PCR troubleshooting - (reply: 2)
  2. Rt pcr - (reply: 4)
  3. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
  4. methylation specifc primers thermal cycle conditions - (reply: 4)
  5. PCR Amplification Issues and Primer dimer - (reply: 4)
  6. How to make PCR analysis using the 22DDCT Method - (reply: 1)
  7. Primer calculation for qPCR - final reaction - (reply: 1)
  8. Sec. structure in primers - (reply: 1)
  9. Changing PCR recipe halfway - (reply: 1)
  10. PCR master mix containing primers and Taq - (reply: 4)
  11. Primer working with some cDNA samples and not other - (reply: 2)
  12. no bands after pcr!!!! - (reply: 4)
  13. Experimental design for RT-PCR - (reply: 8)
  14. Primer dilution - (reply: 7)
  15. why not working msp primers - (reply: 4)
  16. PCR product not migrating from wells...?? - (reply: 6)
  17. Re-PCR my PCR products - (reply: 2)
  18. PCR DIG-labeling kit preblem? - (reply: 1)
  19. pcr - (reply: 1)
  20. how to analysis the BiSearch ePCR result - (reply: 3)
  21. Standardizing cDNA concentration before doing PCR - (reply: 4)
  22. Volume of Primers - (reply: 4)
  23. PCR conditions unknown? - (reply: 10)
  24. PCR anomaly in a mid-range dilution of the template - (reply: 2)
  25. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
  26. MSP_Primer dimers? - (reply: 17)
  27. PCR using very long oligos !!! - (reply: 4)
  28. Primer Designing - (reply: 9)
  29. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  30. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  31. colony pcr, two strong bands - (reply: 4)
  32. Ligation of two PCR products - (reply: 1)
  33. pcr primer design - (reply: 6)
  34. Please help (primer/ probe final volume calculations) - (reply: 1)
  35. Primer design with EcoRI ends - (reply: 2)
  36. Trouble with PCR using ligation mix as template? - (reply: 3)
  37. PCR with mammalian cells as template - (reply: 1)
  38. PCR with overlapping primers only (primer extention) - (reply: 3)
  39. Random chuck of nonsense in middle of PCR - (reply: 6)
  40. RT-PCR analysis - (reply: 1)
  41. PCR DNA bands too large on agarose gel - (reply: 1)
  42. MSP Primer design - (reply: 5)
  43. New to PCR - (reply: 3)
  44. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  45. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
  46. Primer design - (reply: 1)
  47. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)
  48. Primer non specific binding sites - (reply: 7)
  49. Degenerate primers - (reply: 8)
  50. Primers with 2 different annealing temp - (reply: 1)
  51. Can you use same samples for real time pcr 2nd time? - (reply: 2)
  52. Staphylococcus aureus colony PCR - (reply: 1)
  53. Cloning purified PCR product into cells - (reply: 5)
  54. PCR & Western Blot sample preperation - (reply: 2)
  55. PCR & Western Blot sample preperation - (reply: 5)
  56. Y-linker transgene integration site PCR primer verification - (reply: 1)
  57. Non especific band in PCR... - (reply: 2)
  58. I need help with PCR problems - (reply: 5)
  59. Question about baselines in Real-Time PCR - (reply: 3)
  60. Inconsistent colony PCR result - (reply: 2)
  61. A problem with PCR array - (reply: 4)
  62. My PCR suddenly stopped working and I'm losing my mind - (reply: 3)
  63. Concentration of Eva green with different amplicon sizes in a PCR pool - (reply: 1)
  64. labX offers on realtime PCR machines - (reply: 4)
  65. Could any of you help with an explanation to these vague real time PCR curves &# - (reply: 2)
  66. polymerase with or without proofreading activity used in study nucleotide polymo - (reply: 1)
  67. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  68. Suggest best micropipette for PCR purpose... - (reply: 1)
  69. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  70. Plasmid Amplification Issue - (reply: 6)
  71. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  72. primer design for a gene - (reply: 3)
  73. large bands in all pcr reactions including negative control - (reply: 1)
  74. confirmation for primers dilution - (reply: 10)
  75. Problems regarding amplification of my gene - (reply: 3)
  76. PCR working, qPCR is *not*. - (reply: 6)
  77. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  78. Overlapping PCR, need help - (reply: 5)
  79. How does RT-PCR work? - (reply: 5)
  80. Denatured plasmid for pcr - (reply: 1)
  81. Possible primer dimer problem..Need solution!!! - (reply: 2)
  82. confusing with where to put primers and which ordination - (reply: 4)
  83. Help with primer concentration for sequencing - (reply: 1)
  84. PCR troubleshoot - (reply: 6)
  85. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  86. PCR Products not run well in the GEL - (reply: 1)
  87. PCR solution calculus - (reply: 4)
  88. Amplifying from pcr products - (reply: 7)
  89. Consistancy issue with PCR block - (reply: 1)
  90. Queries regarding PCR - (reply: 3)
  91. Design the primers - (reply: 10)
  92. Calculation of Taq Polymerase - (reply: 3)
  93. Best Taq for colony PCR? - (reply: 5)
  94. How two fragments joint together when doing fusion PCR? - (reply: 4)
  95. How to test my milleq water in q PCR about contaminants - (reply: 3)
  96. Mutagenesis PCR - (reply: 9)
  97. real time PCR primer design - (reply: 1)
  98. The PCR gods are frowning upon me - (reply: 9)
  99. PCR after T4 ligation? - (reply: 3)
  100. primers for cloning - (reply: 1)
  101. Degenerated Primers and their problems - (reply: 1)
  102. Sonication of small PCR amplicon - (reply: 1)
  103. primer design with restriction enzyme - (reply: 1)
  104. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  105. primer dilution help - (reply: 1)
  106. Testing primers on 'unknown' tissue - (reply: 1)
  107. How to remove inhibitory substances in PCR? - (reply: 2)
  108. need some advise about PCR of PKD1-human gene - (reply: 7)
  109. puzzled with PCR outcome after BS treatment - (reply: 2)
  110. nested PCR for low viral load- HBV patient sample - (reply: 2)
  111. troubleshooting stubborn PCR - (reply: 6)
  112. Measuring pcr fragments in a gel - (reply: 1)
  113. Amplification in HK gene but not for target gene - (reply: 3)
  114. could you help me with my stem loop RT primer? - (reply: 1)
  115. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  116. How long can I store at - 30°C my PCR mix? - (reply: 6)
  117. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  118. RT-PCR help - (reply: 2)
  119. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  120. PCR Temperature change control malfunctioning - (reply: 1)
  121. excess amount of primers - (reply: 2)
  122. Dehydrating and Reconstituting primers - (reply: 15)
  123. Trouble with PCR of short sequence - (reply: 3)
  124. Setting the temperature range for gradient PCR - (reply: 3)
  125. KIR gene promoters for MSP primers design - (reply: 2)
  126. Biotin-streptavidin signal amplification - (reply: 2)
  127. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  128. Amplification curve in the negative control samples - (reply: 7)
  129. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  130. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  131. Electrophoresis after PCR : too many bands - (reply: 6)
  132. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  133. Primer as limiting reagent in PCR reaction - (reply: 2)
  134. Primer Design for RNA probes - (reply: 2)
  135. Real time PCR for degraded RNA - (reply: 1)
  136. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  137. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  138. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  139. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  140. Nested BSP Primer Design - (reply: 6)
  141. website for primer design - (reply: 1)
  142. PCR Efficiency over 150%! - (reply: 1)
  143. problem of amplification - (reply: 2)
  144. PCR not working - (reply: 11)
  145. How to do a primer dilution - (reply: 10)
  146. Troubles with Fusion PCR - (reply: 1)
  147. Primer design and alternative transcripts - (reply: 2)
  148. PCR and sequencing of genomic DNA - (reply: 5)
  149. qPCR amplification - (reply: 4)
  150. NESTED PCR - (reply: 6)
  151. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  152. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  153. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  154. Digestion necessary after PCR? - (reply: 9)
  155. Inverse PCR product selection - (reply: 2)
  156. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  157. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  158. how to hasten real-time PCR amplifications - (reply: 2)
  159. Please please help me with my Phusion PCR. - (reply: 5)
  160. PCR - (reply: 1)
  161. NCBI Primer Design - Stringency Issues - (reply: 3)
  162. Different primer concentration in qPCR - (reply: 1)
  163. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  164. help in long pcr - (reply: 1)
  165. PCR inhibitor in template DNA - (reply: 3)
  166. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  167. Problem with Real-time PCR results analysis - (reply: 1)
  168. Primer Specificity: Testing only one primer - (reply: 4)
  169. PCR from protozoa DNA - (reply: 3)
  170. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 6)
  171. tool for comparing many primers pairs - (reply: 4)
  172. PCR that leads to protein synthesis - (reply: 18)
  173. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  174. Use of DMSO in General PCR - (reply: 1)
  175. PCR product size confusion - (reply: 3)
  176. Pcr primers - (reply: 7)
  177. Concentration specification in PCR - (reply: 3)
  178. Guanidine isothiocyanate in PCR - (reply: 1)
  179. Primers have worked well but now getting primer dimers? - (reply: 2)
  180. I cannot design primers on exon-exon junction - (reply: 2)
  181. DNA Quantification of PCR Products - (reply: 2)
  182. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  183. Problem for PCR - (reply: 9)
  184. Designing primers in UTRs - (reply: 1)
  185. Multiplex PCR - (reply: 1)
  186. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  187. primer design@ buy? - (reply: 2)
  188. Trouble with overlap extension pcr - (reply: 3)
  189. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  190. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  191. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  192. Question about the RT PCR - (reply: 3)
  193. faint dna band after pcr purification - (reply: 1)
  194. PCR ready mixes with long shelf lives - (reply: 4)
  195. Troubleshooting: Inverse PCR - (reply: 3)
  196. How to design primer to amplify genomic DNA? - (reply: 3)
  197. Overlap PCR, need help - (reply: 11)
  198. Internal control for miRNA RT-PCR - (reply: 1)
  199. Primers - (reply: 1)
  200. Primers mix - (reply: 2)
  201. question about RNA concentration for real time PCR - (reply: 1)
  202. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  203. PCR products sizes and DNA ladder - (reply: 7)
  204. Having problem with primers for qPCR - (reply: 4)
  205. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  206. PCR product sequencing - (reply: 3)
  207. Bad fragment amplification - (reply: 4)
  208. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  209. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  210. No amplification with TRAPEZE kit! - (reply: 1)
  211. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  212. Stargazer PCR problems - (reply: 3)
  213. General PCR discussion - (reply: 8)
  214. Real time. No amplification but hight flourescence - (reply: 1)
  215. Real time PCR doubt - (reply: 5)
  216. PCR Profile for ligation - (reply: 3)
  217. protocol to relieve melanin inhibition of PCR - (reply: 4)
  218. No bands despite different primers and conditions and TAqs - (reply: 8)
  219. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  220. Equimolar Mix Primer - (reply: 8)
  221. Wrong PCR product - (reply: 2)
  222. Colony PCR positive and Digestion negative????? - (reply: 11)
  223. Primer design - (reply: 5)
  224. storage for lyophilized primers - (reply: 1)
  225. PCR GAPDH gene - (reply: 1)
  226. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  227. Quantitative RT-PCR statistics help - (reply: 1)
  228. Dimerization of PCR product - (reply: 4)
  229. PCR Primer trouble - (reply: 2)
  230. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  231. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  232. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  233. finding the corrosponding primers - (reply: 2)
  234. Gene sequence for Real time PCR - (reply: 2)
  235. non-reproducible PCR results with cDNA as template - (reply: 1)
  236. Ligation of PCR fragments - (reply: 11)
  237. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  238. RNA extraction for RT-PCR - (reply: 3)
  239. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  240. problems with gDNA doing real time PCR in yeast - (reply: 2)
  241. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  242. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  243. How to get rid of bands in PCR negative control - (reply: 10)
  244. T7 and M13 primers two band amplification - (reply: 3)
  245. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  246. Strange PCR problem - (reply: 2)
  247. Question about Double Digestion followed by PCR amplification - (reply: 2)
  248. PCR problem - (reply: 2)
  249. PCR machine not working properly - (reply: 1)
  250. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  251. PCR gene specific amplification problem - (reply: 3)
  252. Failure SYBRGREEN PCR - (reply: 4)
  253. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  254. without RE site in PCR product - (reply: 5)
  255. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  256. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  257. real-time pcr non reproducible - (reply: 4)
  258. Strange amplification plots with high Ct variability - (reply: 1)
  259. How big a role does mixing play in PCR - (reply: 1)
  260. Melting curve is irregular for primer optimization - (reply: 5)
  261. Designing primers for ABO blood groups - (reply: 1)
  262. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  263. RT-PCR primer design - (reply: 7)
  264. How to amplify very short PCR template - (reply: 4)
  265. Is this primer okay? - (reply: 4)
  266. PCR amplification of large template - (reply: 1)
  267. PCR insert - in frame? - (reply: 2)
  268. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  269. smear in gel electrophoresis after PCR - (reply: 2)
  270. Can I use Primer as template? - (reply: 1)
  271. Whole plasmid amplification by PCR - (reply: 2)
  272. PCR product as standard curve template - (reply: 6)
  273. colony PCR after transformation - (reply: 1)
  274. PCR machine - (reply: 3)
  275. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  276. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  277. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  278. Resuspending primers calculation - (reply: 4)
  279. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  280. PCR RFLP is PCR product purification necessary? - (reply: 3)
  281. Mystery in my PCR - (reply: 5)
  282. Whole genome amplification from cDNA? - (reply: 4)
  283. What do you use to check primer secondary structure? - (reply: 3)
  284. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  285. PCR product as template for in vitro transcription - (reply: 1)
  286. Problem with 3 step PCR - (reply: 3)
  287. mutagenic primers with very high GC content. - (reply: 3)
  288. Scaling up PCR to get more DNA - (reply: 5)
  289. PCR to get 10kbp product - (reply: 4)
  290. site-directed mutagenesis primer Tm - (reply: 4)
  291. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  292. Random vs oligo primer in preamplification RT - (reply: 1)
  293. how does polymerase stop at the required length - (reply: 1)
  294. Chip pcr. are there inespecifics? - (reply: 1)
  295. Primer concentration - stupid question - (reply: 3)
  296. Primer Design, help i´m New - (reply: 4)
  297. No DNA after PCR product purification - (reply: 9)
  298. Sensitivity of RT-PCR and qPCR - (reply: 4)
  299. RT-qPCR primer problem - (reply: 4)
  300. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  301. real time PCR trouble - (reply: 3)
  302. removal of ethanol in PCR product purification - (reply: 5)
  303. Crazy real time PCR curve - (reply: 4)
  304. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  305. High fidelity PCR trouble shooting - (reply: 2)
  306. Designing cloning primers for DNMT - (reply: 2)
  307. Reproducible Non-Specific PCR Product - (reply: 2)
  308. Are these primer products good enough for qPCR? - (reply: 3)
  309. Can't get PCR with large overhang primers to work - (reply: 8)
  310. Primer Efficiency across runs - (reply: 1)
  311. Question about pipet tips for PCR and rtPCR - (reply: 4)
  312. UNG in PCR - (reply: 1)
  313. Cause of random samples failing PCR? - (reply: 2)
  314. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  315. pcr 10X buffer preparation - (reply: 2)
  316. how much template do i need for pcr? - (reply: 5)
  317. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  318. Problems with crossover PCR - (reply: 2)
  319. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  320. Designing PCR Primers for cloning - (reply: 17)
  321. Creating primers to add restriction sites to vector backbone - (reply: 7)
  322. PCR DNA Concentration - (reply: 1)
  323. RT-PCR product- no band - (reply: 4)
  324. Some primers dose not work - (reply: 5)
  325. Understanding RACE PCR - (reply: 1)
  326. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  327. Wierd Bands after PCR....Confused - (reply: 9)
  328. Query regarding primers for quick change mutagenesis - (reply: 3)
  329. PCR with Plasmid recovered from filter paper - (reply: 6)
  330. PCR amplified product size - (reply: 5)
  331. the storage time for primers - (reply: 9)
  332. Annealing temperature for PCR - (reply: 8)
  333. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  334. Nested PCR - (reply: 2)
  335. random primers or oligodT - (reply: 4)
  336. No amplification during RT-PCR - (reply: 4)
  337. Sequencing RT-PCR product - (reply: 3)
  338. Low yield PCR product after gel purification - (reply: 8)
  339. To design or use published primers? - (reply: 4)
  340. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  341. PCR primer usage (Clonning & cDNA) - (reply: 2)
  342. problem in cloning PCR primer design with restriction site - (reply: 4)
  343. Amplification with U primers, but not with M primers - (reply: 1)
  344. Taq polymerase - (reply: 7)
  345. His tag introduction in to gene and primer design - (reply: 2)
  346. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  347. Which High-Fidelity polymerase is better? - (reply: 2)
  348. polymerase to use for cloning - (reply: 4)
  349. Problem amplifying viral gene - (reply: 5)
  350. Adding tag using overlapping PCR - (reply: 2)
  351. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  352. Design primer from incomplete sequence... - (reply: 2)
  353. Design primer from incomplete sequence... - (reply: 2)
  354. How to perform colony PCR - (reply: 1)
  355. Primer designing for Methylation - (reply: 6)
  356. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  357. cDNA amplification problem - (reply: 4)
  358. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  359. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  360. PCR product running on agarose gel - (reply: 32)
  361. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  362. No bands in PCR after DNase treatment - (reply: 4)
  363. PCR amplification with high fidelity enzyme - (reply: 1)
  364. PCR HELP!!!! - (reply: 1)
  365. Overlap PCR problem - (reply: 5)
  366. PCR and restriction enzyme digestion - (reply: 3)
  367. Bisulfite PCR and cloning - (reply: 5)
  368. PCR product purification - (reply: 4)
  369. Overlapping sequence PCR primers - (reply: 1)
  370. restricted PCR plasmid runs slower - (reply: 2)
  371. BSP PCR primer design explained - (reply: 11)
  372. Bisulfite sequencing PCR worked - (reply: 5)
  373. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  374. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  375. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  376. doing PCR using PCR product , help plz !! - (reply: 5)
  377. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  378. good 16S univ. primer for qPCR? - (reply: 3)
  379. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  380. Amplification of CAG promoter problems - (reply: 4)
  381. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  382. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  383. I need help for designing a pair of primers for this sequence. - (reply: 1)
  384. PCR not working for 5 Aza treated DNA - (reply: 3)
  385. Differentiating mouse from human cells with PCR - (reply: 5)
  386. Differentiate between RT-PCR and PCR - (reply: 3)
  387. PCR band slightly BELOW expected length ?! - (reply: 14)
  388. long primers PCR - (reply: 4)
  389. unexpected band in PCR with plasmid - (reply: 4)
  390. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  391. DNA polymerase for GC rich template? - (reply: 5)
  392. Sequencing Primers and Plasmids - (reply: 3)
  393. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  394. DNA extraction - PCR Problem - (reply: 3)
  395. Primers to amplify Kan operon from pet28 - (reply: 2)
  396. PCR product biotinylation - (reply: 3)
  397. Tagged primers and a second PCR targetting the tags - (reply: 5)
  398. cloning pcr insert into plasmid - (reply: 2)
  399. Barcode generator for PCR primers - (reply: 2)
  400. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  401. Barcoding PCR products - (reply: 18)
  402. Biotinylated primers - (reply: 4)
  403. role of water in PCR - (reply: 3)
  404. HIV gene- PCR - (reply: 3)
  405. Real time PCR melting curve - (reply: 2)
  406. BSA in PCR reaction - (reply: 1)
  407. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  408. Cannot see insert with colony pcr - (reply: 6)
  409. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  410. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  411. How to Join two PCR products - (reply: 3)
  412. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  413. What's wrong with my PCR - (reply: 5)
  414. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  415. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  416. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  417. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  418. Cp, but no amplification - (reply: 2)
  419. pcr product digestion problem - (reply: 4)
  420. Low PCR product - (reply: 7)
  421. Control primers for bisulfite-converted DNA - (reply: 3)
  422. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  423. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  424. wrong dntp concentration - (reply: 2)
  425. Third round PCR - (reply: 2)
  426. long fragment PCR - (reply: 2)
  427. Best proof-reading polymerase? - (reply: 13)
  428. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  429. Q regarding primer mix for MSP!! - (reply: 4)
  430. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  431. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  432. I need to break this cycle of PCR issues - (reply: 13)
  433. Questions / Help with Fusion PCR - (reply: 8)
  434. Controls for RT-PCR reactions - (reply: 2)
  435. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  436. BSP Primers with M13 Tails - (reply: 1)
  437. Detection of T7 RNA polymerase by WB - (reply: 6)
  438. Multiplex PCR - (reply: 33)
  439. issues when designing PCR primers using PRIMER3 - (reply: 8)
  440. Primer catalog/databasing - (reply: 1)
  441. Nonrepetitive nested PCR - (reply: 2)
  442. Primer Blast - (reply: 6)
  443. Help designing primers for use in In-fushion cloning - (reply: 4)
  444. PCR internal control - (reply: 2)
  445. Primers Freeze-Thaw - (reply: 16)
  446. TDNA Genotyping PCR program - (reply: 1)
  447. primer design using 16s rRNA - (reply: 1)
  448. question about PCR and cloning - (reply: 1)
  449. PCR - (reply: 2)
  450. nonspecific band at 50bp in PCR - (reply: 9)
  451. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  452. PCR master mix - (reply: 7)
  453. Maximum PCR product Tm - (reply: 6)
  454. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  455. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  456. PCR & Southern blotting - (reply: 1)
  457. Bisulfite PCR Question - (reply: 2)
  458. Primer Design HELP! - (reply: 3)
  459. Help with sequencing of a 120bp PCR product. - (reply: 9)
  460. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  461. How are Primers made? - (reply: 9)
  462. Long Non-Specific PCR Products - (reply: 5)
  463. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  464. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  465. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  466. PCR calculation - (reply: 1)
  467. Colony PCR and digestion with KpnI - (reply: 10)
  468. designing methylation specific primers - (reply: 3)
  469. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  470. PCR - consistent false positive results - (reply: 6)
  471. PCR no amplification - (reply: 15)
  472. How to quantify virus by PCR - (reply: 12)
  473. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  474. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  475. Pcr product size determination for a fusion gene - (reply: 8)
  476. Quantify DNA before ChIP PCR - (reply: 3)
  477. Primer Annealing Temperatures - (reply: 24)
  478. my pcr product is larger than expected - (reply: 4)
  479. Question about PCR - DNA or RNA as template - (reply: 4)
  480. pcr purification - (reply: 1)
  481. pcr purification doubt - (reply: 4)
  482. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  483. PCR band moves lower than its size on agarose gel - (reply: 3)
  484. same primers, different product in conventional PCR and qPCR - (reply: 6)
  485. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  486. pcr problem - (reply: 6)
  487. NEB Q5 polymerase works very well - (reply: 1)
  488. Primers amplify genomic DNA but not cDNA - (reply: 2)
  489. pcr purification - (reply: 1)
  490. storing PCR product overnight - (reply: 2)
  491. Primer BLAST - (reply: 1)
  492. Bands in negative control PCR - (reply: 2)
  493. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  494. problem with pcr mutagenesis - (reply: 10)
  495. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  496. Use PCR purification kit to purify restriction digestion - (reply: 2)
  497. PCR primer design - published primers trustable? - (reply: 8)
  498. Polymerase for E. coli screenings - (reply: 5)
  499. complicated RT-PCR issues - (reply: 4)
  500. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  501. Can we use degenerate bases in BSP primers? - (reply: 1)
  502. Quick change mutagenesis No PCR product visible - (reply: 2)
  503. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  504. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  505. Can anyone help with my Bisulfite PCR? - (reply: 2)
  506. How to check primer sequences using BLAST and other tools? - (reply: 2)
  507. real time PCR analysis in patient samples - (reply: 2)
  508. carpet in gels for PCR products - (reply: 2)
  509. Primer Check? - (reply: 2)
  510. Sequencing of the PCR production in BSP - (reply: 1)
  511. amplification of 14Kb - (reply: 1)
  512. Validation of PCR primers/ probes - (reply: 6)
  513. Taq polymerase for MSP - (reply: 1)
  514. Real time PCR sudenly not working - (reply: 1)
  515. Buffer-composition One-Step RT-PCR - (reply: 4)
  516. Two reverse primer sequences for a single forward primer - (reply: 1)
  517. PCR with more than one primer - (reply: 1)
  518. RAPD - PCR problem - (reply: 5)
  519. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  520. How to best store PCR product? - (reply: 2)
  521. RT-PCR primer design - (reply: 1)
  522. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  523. Odd gel run following PCR - (reply: 28)
  524. Qiagen PCR Array Reagents? - (reply: 1)
  525. No or very weak band using 16s primers - (reply: 1)
  526. No PCR amplification with b-actin primers - (reply: 1)
  527. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  528. Site-directed, PCR works but no colonies - (reply: 2)
  529. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  530. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  531. No PCR amplified with long primers - (reply: 10)
  532. PCR optimization: PCR vs qPCR - (reply: 4)
  533. should I do blunt end my pcr product before ligation - (reply: 2)
  534. PCR smear for genomic samples - (reply: 4)
  535. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  536. another primer dilution question - (reply: 2)
  537. i need help with virus pcr - (reply: 2)
  538. Primer Efficiency - (reply: 2)
  539. Experimental set up for qRT PCR - (reply: 13)
  540. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  541. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  542. How should I optimize my PCR - (reply: 2)
  543. Second round of PCR after gel extraction fails miserably - (reply: 3)
  544. an effective way to do a yeast colony pcr - (reply: 2)
  545. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  546. urgent help plz with RT-PCR - (reply: 3)
  547. oligo(dt) 15 vs random primers - (reply: 3)
  548. Help to optain a long PCR produc - (reply: 3)
  549. Multiple bands in eluted PCR product - (reply: 1)
  550. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  551. Problems with two step RT-PCR - (reply: 2)
  552. Getting genomic DNA for PCR - (reply: 4)
  553. primer dilutions - (reply: 4)
  554. Genotyping PCR primers - (reply: 1)
  555. Conventional PCR - (reply: 1)
  556. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  557. PCR product loss on cleanup - (reply: 2)
  558. MSP Primers not working - (reply: 6)
  559. Primers too dilute - (reply: 1)
  560. Amplification curves jagged - (reply: 1)
  561. Cloning HELP- Possible Primer issues - (reply: 6)
  562. No PCR product in site-directed mutagenesis - (reply: 4)
  563. Giardia/Crypto real time PCR late amplification - (reply: 1)
  564. How to dilute the template DNA for PCR - (reply: 7)
  565. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  566. Need help for PCR - (reply: 5)
  567. PCR primer pairs - university exam - (reply: 7)
  568. unespecific band PCR from mouse genomic DNA - (reply: 9)
  569. Help with primer design through PRIMER3 - (reply: 4)
  570. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  571. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  572. Plasmid vs cDNA amplification by PCR - (reply: 2)
  573. Tm of primers - prediction and verification - (reply: 1)
  574. PCR cloning...help! - (reply: 5)
  575. RT-PCR contamination in negative control. - (reply: 1)
  576. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  577. how to design primers for 16sr RNA - (reply: 24)
  578. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  579. Help with primer design - (reply: 3)
  580. Primer Tm calculation. - (reply: 1)
  581. low concentration of purified PCR product - (reply: 2)
  582. PCR smearing and...problems! - (reply: 7)
  583. Running Primers on Agarose Gel - (reply: 6)
  584. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  585. universal primers for PCR ribotyping - (reply: 6)
  586. will the template of PCR be added A ? - (reply: 2)
  587. Colony PCR not working - (reply: 4)
  588. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  589. Primer design: Tm above or below Ta? - (reply: 5)
  590. Oligo primers for shRNA construction - (reply: 1)
  591. Design primers for expression cloning - (reply: 11)
  592. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  593. primer dilution query - (reply: 2)
  594. Real Time PCR polymerase enzyme - (reply: 3)
  595. Estimating size of PCR products in a agarose gel - (reply: 4)
  596. Sequencing of PCR product - (reply: 4)
  597. PCR contamination problem - (reply: 2)
  598. Using BLAST to check primers - (reply: 1)
  599. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  600. What is the function of a third primer in a PCR mix?? - (reply: 2)
  601. How can we design a primer ? step by step method - (reply: 4)
  602. cDNA synthesis or RT-PCR - (reply: 3)
  603. PCR on cDNA and SGamplification??? - (reply: 1)
  604. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  605. real time PCR protocol - controls? - (reply: 2)
  606. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  607. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  608. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  609. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  610. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  611. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  612. PCR and UV spectrophotometry - (reply: 3)
  613. RT-PCR in a single tube - (reply: 3)
  614. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  615. annealing temperature for 3 primer pairs? - (reply: 4)
  616. PCR Reaction - (reply: 3)
  617. Impact of primer sequence on TA cloning - (reply: 3)
  618. problems with Bisulfite PCR - (reply: 1)
  619. low pcr product - (reply: 3)
  620. overlapping PCR protocol - (reply: 12)
  621. PCR to amplify my insert not working - (reply: 6)
  622. Problem with PCR amplification- high 3' stability - (reply: 3)
  623. Home made real-time PCR problem - (reply: 4)
  624. High RNase concentration interfering with PCR? - (reply: 8)
  625. Pcr primers - (reply: 2)
  626. PCR failure from yeast genomic DNA - (reply: 1)
  627. failure of PCR from yeast genomic DNA - (reply: 1)
  628. RT-Q PCR primer design question - (reply: 2)
  629. high MW dimers - (reply: 2)
  630. Strange RT-PCR amplification plots - (reply: 9)
  631. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  632. What products will I get from this pcr? - (reply: 1)
  633. PCR buffer without Tris - (reply: 1)
  634. PCR Water - (reply: 4)
  635. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  636. Which Brand of real-time PCR machine - (reply: 6)
  637. Problem with PCR - large extra bands - (reply: 3)
  638. big differnece in my primers Tm - (reply: 2)
  639. Designing primers for the ORFs - (reply: 9)
  640. Two-Step PCR - (reply: 2)
  641. Gradient PCR - (reply: 2)
  642. PCR with phophorylated primers - (reply: 1)
  643. Problem with New qPCR primers - help! - (reply: 6)
  644. Designing primers for miRNA located on minus strand. - (reply: 2)
  645. Plasmid sequencing without primers - (reply: 1)
  646. Smallest insert in PCR - (reply: 6)
  647. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  648. loop-mediated amplification - (reply: 2)
  649. Blunt end PCR product ligation - (reply: 1)
  650. Help with high Ct values for Real-Time PCR - (reply: 1)
  651. Genotyping by allele specific PCR - (reply: 6)
  652. RT-PCR primers and the poly(A) tail - (reply: 2)
  653. NO Insert found in the Colony PCR - (reply: 15)
  654. No band in PCR - (reply: 5)
  655. overlapping primer - (reply: 1)
  656. PCR primers help - (reply: 5)
  657. PCR smear after one-month storage - (reply: 2)
  658. Primer dimers - (reply: 2)
  659. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  660. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  661. Simple question of dNTP's for PCR - (reply: 5)
  662. Need help for PCR for AT rich gene - (reply: 3)
  663. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  664. Primer validation and PCR efficiency - (reply: 4)
  665. How to calculate the price of PCR test per sample? - (reply: 6)
  666. Contamination in negative control of PCR (No template control) - (reply: 6)
  667. cloning with nested pcr - (reply: 3)
  668. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  669. PCR is amplifying non-specific fragment - (reply: 6)
  670. ChIP amplification problems - (reply: 1)
  671. real time RT-PCR problems - (reply: 2)
  672. should I design primers spanning both intron and exon - (reply: 2)
  673. RT-PCR - (reply: 1)
  674. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  675. Template DNA (plasmid) concentration for PCR - (reply: 4)
  676. PCR with a very long and a short primer - (reply: 5)
  677. Ligation of 16kb PCR product - (reply: 5)
  678. Multiplex PCR does not work - (reply: 3)
  679. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  680. Problem with genomic PCR (2.4 kb) - (reply: 1)
  681. Comparison between two primers pairs? Is it possible? - (reply: 3)
  682. Rt-PCR primer design - (reply: 7)
  683. Length of non-binding sequence in primers with RE site - (reply: 6)
  684. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  685. Getting a smaller PCR product than required - (reply: 6)
  686. Difficult sequences in PCR - (reply: 2)
  687. why is real time pcr curve like this? - (reply: 3)
  688. PCR inconsistency - (reply: 2)
  689. Orientation of primers - (reply: 2)
  690. PCR, neg control & no. of cycles - (reply: 2)
  691. Strange PCR result from genomic DNA - (reply: 3)
  692. Bright bands of PCR products stick in gel wells - (reply: 1)
  693. methylation analysis with MS-HRM (primer design) - (reply: 1)
  694. whole plasmid pcr? - (reply: 6)
  695. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  696. salts in PCR - (reply: 4)
  697. Strange amplification plot - (reply: 4)
  698. Conventional PCR of different dilutions - (reply: 1)
  699. primer resuspension - (reply: 2)
  700. Principles of overlap PCR - (reply: 7)
  701. ligation of pcr product into expression vector - (reply: 4)
  702. Unable to PCR!!!! - (reply: 3)
  703. Help designing primers with restriction sites - (reply: 3)
  704. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  705. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  706. Abnormal amplification curve - (reply: 1)
  707. Problem with Semiquantitative PCR - (reply: 4)
  708. Advice on optimising bisulfite PCR sought - (reply: 2)
  709. Help: I am loosing the DNA during PCR purification - (reply: 7)
  710. PCR primer with no extra DNA end - (reply: 5)
  711. PCR colonies of Pichia pastoris - (reply: 1)
  712. ROX reference dye for quantitative RT-PCR - (reply: 1)
  713. PCR problem - (reply: 9)
  714. why PCR product smeared? - (reply: 2)
  715. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  716. silincing box, no amplification - (reply: 3)
  717. Comparative semiquantitative RT-PCR - (reply: 2)
  718. Bands in my PCR Controls - (reply: 3)
  719. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  720. Strange band in colony PCR - (reply: 2)
  721. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  722. Multiplex PCR - (reply: 12)
  723. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  724. PYO pcr stopped working. - (reply: 1)
  725. Bands seen with qPCR missing in regular PCR - (reply: 5)
  726. checking msp primers - (reply: 2)
  727. PCR product one day, none the next day - (reply: 4)
  728. PCR on colony - (reply: 10)
  729. PCR reaction ISSUES???? need help - (reply: 4)
  730. PCR using CDNA as template - (reply: 2)
  731. RT-PCR doesn't work with all RNA used - (reply: 1)
  732. Need help amplifying repeating sequence. - (reply: 5)
  733. Real Time PCR 101...help! - (reply: 1)
  734. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  735. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  736. Another primer dimers problem - (reply: 23)
  737. Colony PCR Problem!!!! - (reply: 5)
  738. using PCR product as standard template - (reply: 3)
  739. PCR kit - (reply: 7)
  740. Make additional cycles with an already finished pcr product - (reply: 3)
  741. Amplification in water control but not in samples - (reply: 3)
  742. PCR products form with DNA, but not with c-DNA - (reply: 2)
  743. question about pcr amplification efficiency? - (reply: 1)
  744. DNA polymerase: recombinant or native? - (reply: 3)
  745. pcr clean-up fail? - (reply: 1)
  746. MSP U primers very difficult to amplify... - (reply: 4)
  747. No gene amplification, 18s amplification is fine - (reply: 1)
  748. ChIP-qPCR gives odd amplification plot - (reply: 5)
  749. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  750. sequential cloning of multiple PCR products - how to do? (reply: 3)
  751. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  752. Primer design - (reply: 3)
  753. Tomato Actin PCR+Images included - (reply: 2)
  754. single primer PCR - (reply: 5)
  755. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  756. cDNA synthesis + amplification - (reply: 2)
  757. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  758. LacZ PCR Problems - (reply: 1)
  759. cells stably expressing T7 RNA polymerase - (reply: 1)
  760. Primer concentration - (reply: 4)
  761. nested PCR with low target DNA? - (reply: 2)
  762. RT-PCR calibrator - (reply: 2)
  763. RNA - extraction and pcr (reply: 1)
  764. problem in pcr - (reply: 2)
  765. Can someone recommend me a virtual PCR software? - (reply: 6)
  766. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  767. Primer 3' mismatch - Strange experience (reply: 9)
  768. real-time PCR after restriction enzyme digestion - (reply: 6)
  769. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  770. Adding loading/tracking dye to PCR mix? - (reply: 10)
  771. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  772. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  773. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  774. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  775. how to get rid of non specific bands in PCR - (reply: 1)
  776. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  777. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  778. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  779. PCR cloning problem - could not get colony (reply: 4)
  780. pcr contamination - (reply: 4)
  781. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  782. Large Non-specfic bands in PCR - (reply: 3)
  783. PCR clonning - (reply: 3)
  784. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  785. 2 bands in blue colony PCR - (reply: 5)
  786. Primer dilution --> problem.. - (reply: 16)
  787. Help! Smear in Real-Time PCR Product - (reply: 1)
  788. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  789. Sybr green RT-qPCR primer - (reply: 1)
  790. Sybr green RT-qPCR primer - (reply: 1)
  791. primer design - microalgae (reply: 2)
  792. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  793. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  794. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  795. PCR yield in ng/ul - (reply: 1)
  796. No Amplification in PCR - (reply: 4)
  797. Real time PCR and percentage loss - (reply: 1)
  798. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  799. Problem with smear in PCR with certain templates - (reply: 4)
  800. Real time PCR in the presence of heme - (reply: 3)
  801. issues in PCR amplification - (reply: 2)
  802. Problems with PCR in general - (reply: 1)
  803. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  804. how to overcome primer contamination - need protocol (reply: 11)
  805. RT-PCR primer design - Primer design and evaluation (reply: 7)
  806. CHIP analysis by PCR - (reply: 4)
  807. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  808. Primer,probes dilution - (reply: 3)
  809. Splice varient quantification in RT real time PCR? - (reply: 1)
  810. negative control for msp pcr - (reply: 2)
  811. Calculating geometric mean for real-time PCR - (reply: 5)
  812. template transfer? - how does the rna polymerase switches template? (reply: 1)
  813. PCR Gel nonspecific band - (reply: 13)
  814. semiquantitative pcr - (reply: 1)
  815. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  816. PCR: Cloning Primers - (reply: 8)
  817. PCR product - (reply: 5)
  818. pcr amplify an insert in plasmid? - (reply: 2)
  819. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  820. Exon Spanning PCR Primers - (reply: 1)
  821. Colony PCR - (reply: 1)
  822. PCR to confirm double crossover - (reply: 1)
  823. Primer sequences - (reply: 1)
  824. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  825. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  826. PCR Primers - (reply: 2)
  827. Cleaning up RT-PCR product before sequencing - (reply: 1)
  828. Template Amount for PCR Amplification - (reply: 6)
  829. How to blast methylation specific and unspecific primers - (reply: 1)
  830. RT-PCR cDNA synthesis - (reply: 6)
  831. problems amplifying from cDNA - (reply: 4)
  832. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  833. No DNA after PCR purification with QiaQuick? - (reply: 4)
  834. restriction digestion against colony PCR - (reply: 1)
  835. How to check primers are of correct sequence or not? - (reply: 2)
  836. Primers... - (reply: 1)
  837. When are primer dimers a problem? - (reply: 1)
  838. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  839. PCR: always got band in the negative control - (reply: 8)
  840. primer with higher melting temperature - (reply: 2)
  841. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  842. Problems designing primers for BSP - (reply: 2)
  843. qPCR primer design - possible off-target (reply: 1)
  844. Primer dimer formation and real-time PCR - (reply: 7)
  845. Identify PCR products - (reply: 3)
  846. PCR genomic DNA using cell lysate - (reply: 1)
  847. Maximum size of overhangs in PCR - (reply: 6)
  848. Colony PCR doesn't work anymore - (reply: 5)
  849. RT-PCR t-test, and ANOVA - (reply: 1)
  850. Primer Design Urgent! - (reply: 1)
  851. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  852. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  853. PCR of microRNA first strand cDNA - (reply: 1)
  854. how to know my primer's sense ??? - help ! (reply: 2)
  855. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  856. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  857. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  858. intron spanning primers for non model organisms - (reply: 2)
  859. Primer design - (reply: 2)
  860. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  861. qPCR amplification interference fixed with freezing - (reply: 6)
  862. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  863. software for quantifing PCR product band - (reply: 1)
  864. Multiple bands from purified PCR product - (reply: 3)
  865. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  866. Doing PCR on Nebulized DNA - (reply: 2)
  867. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  868. RT-PCR - (reply: 9)
  869. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  870. inverse pcr - (reply: 2)
  871. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  872. Primer seq - (reply: 2)
  873. Urgent: should you dry PCR product - (reply: 13)
  874. PCR product size - (reply: 6)
  875. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  876. oligo dt and random primer - (reply: 3)
  877. mRNA Search for RT-PCR (U to T) - (reply: 2)
  878. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  879. PCR Mastermix - (reply: 4)
  880. PCR triplicates versus one reaction - differences? (reply: 17)
  881. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  882. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  883. Primers and self and hetero dimers - (reply: 1)
  884. Really weird amplification curves - (reply: 1)
  885. Primers for qPCR - (reply: 3)
  886. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  887. RT-PCR primer - (reply: 3)
  888. PCR problem - basic PCR for plasmid amplification (reply: 2)
  889. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  890. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  891. Unpredictablity of PCR product - (reply: 5)
  892. total cDNA amplification by PCR - (reply: 2)
  893. Detection limit of a conventional PCR - (reply: 2)
  894. PCR for MULTIPLE mutagenesis - (reply: 4)
  895. Using Sybr green in realtime pcr - (reply: 1)
  896. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  897. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  898. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  899. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  900. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  901. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  902. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  903. Digested PCR product migrate slower than uncut - (reply: 8)
  904. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  905. PCR question - (reply: 2)
  906. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  907. 2 rounds PCR got problem - (reply: 2)
  908. PCR help minus strand - Primer design (reply: 1)
  909. chloramphenicol storage and amplification - (reply: 3)
  910. Smear in long distance PCR - (reply: 39)
  911. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  912. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  913. TOUCH DOWN PCR - (reply: 2)
  914. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  915. PCR stopped working - After changing buffer (reply: 5)
  916. ChIP PCR question - (reply: 33)
  917. PCR protocol questions! - (reply: 2)
  918. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  919. DNA amount calculation for PCR - (reply: 14)
  920. ChIP primer design - (reply: 1)
  921. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  922. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  923. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  924. How to analyze ChIP PCR data - (reply: 10)
  925. Questions regarding RT-PCR optimization - (reply: 2)
  926. number of copies after pcr? - (reply: 1)
  927. PCR double-bands - (reply: 1)
  928. Problems with semi-quatitative PCR - (reply: 1)
  929. Question about Primers - (reply: 3)
  930. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  931. Large fragment amplification failed - (reply: 3)
  932. purification of PCR product for cloning in a vector - (reply: 3)
  933. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  934. PCR reaction calculation - (reply: 2)
  935. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  936. Primer design with a tag - (reply: 4)
  937. no increase in fluorescence in my real time PCR - (reply: 3)
  938. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  939. Help with real-time PCR quantification of miRNA - (reply: 8)
  940. PCR, using gel extracted band as a template - (reply: 4)
  941. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  942. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  943. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  944. Combining forward and reverse primer gives different size on gel - (reply: 2)
  945. Q-PCR and RT-PCR - (reply: 3)
  946. calculating total amount - in a pcr reaction (reply: 4)
  947. Problems with amplifying microRNA - (reply: 3)
  948. PCR of complement genomic DNA - (reply: 3)
  949. primers for fungal identification - (reply: 9)
  950. confusing PCR - (reply: 1)
  951. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  952. PCR for long and repetitive region from genomic DNA - (reply: 4)
  953. difference between PCR primers and sequencing primers - (reply: 1)
  954. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  955. PCR of repeated region - (reply: 1)
  956. Sense and antisense DNA, and primer design - (reply: 6)
  957. PCR bands in NTC but NOT in negative samples - (reply: 2)
  958. housekeeping genes in PCR - (reply: 1)
  959. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  960. Question about RealTime PCR Primer Design - (reply: 1)
  961. Designing a Primer with a RS that has a W - (reply: 1)
  962. Primers stopped working!? - PCR Primers (reply: 6)
  963. NFK beta primers - (reply: 4)
  964. final PCR product - need clarifications (reply: 1)
  965. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  966. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  967. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  968. RT-PCR result !! - (reply: 4)
  969. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  970. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  971. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  972. why genomic can't be use for pcr? - (reply: 2)
  973. Primer design - Primer design (reply: 2)
  974. Re-use unamplified PCR product - (reply: 2)
  975. Reason for odd PCR conditions - (reply: 3)
  976. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  977. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  978. pcr - problem in pcr (reply: 2)
  979. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  980. How to present and analyze these real-time PCR data? - (reply: 2)
  981. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  982. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  983. PCR Master mix - (reply: 2)
  984. cDNA storage and real time RT PCR - (reply: 3)
  985. Real Time PCR Primers and Probes - (reply: 2)
  986. Anyone make their own PCR cloning vector? - (reply: 5)
  987. Stability of PCR T-overhangs - (reply: 1)
  988. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  989. PCR product wrong size! Need help! - (reply: 9)
  990. pooling different pcr cycles? - (reply: 1)
  991. Amplification of concatenated linear ligated fragments - (reply: 4)
  992. Clinical Real-time PCR assay - (reply: 1)
  993. Need help - How we can design primer for miRNA (reply: 1)
  994. primer and promoter sequences - (reply: 1)
  995. Standard curve for real-time PCR - (reply: 2)
  996. designing of primers - (reply: 2)
  997. Primer Tm differences/ PCR - (reply: 1)
  998. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  999. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  1000. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  1001. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  1002. Designing primer to remove his-tag - (reply: 1)
  1003. PCR Primer Dilution from F+R - (reply: 1)
  1004. primer reconstitution - primer reconstitution (reply: 4)
  1005. Not-Quite-Nested PCR - (reply: 2)
  1006. PCR product too short - (reply: 6)
  1007. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  1008. Primer design for insert amplification - Is this correct? (reply: 2)
  1009. got smear on the PCR gel !!! - (reply: 4)
  1010. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  1011. "Unzipped" PCR band - (reply: 3)
  1012. Review on new amplification techniques - (reply: 2)
  1013. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  1014. DOT BLOT for pcr product - No result obtain (reply: 1)
  1015. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  1016. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  1017. HRM primers - (reply: 1)
  1018. PCR amplification with Pfu / quality of DNA - (reply: 4)
  1019. primer design - (reply: 2)
  1020. Is it possible to compare data between real-time PCR plates - (reply: 1)
  1021. Conventional PCR problems - wisamni2004 (reply: 2)
  1022. New to sequencing, primer design - (reply: 2)
  1023. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  1024. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  1025. incorrect/smaller band size for real time PCR - (reply: 1)
  1026. Quickchange mutagenesis primer - (reply: 3)
  1027. pcr / primer theory in bisulfite sequencing - (reply: 4)
  1028. homologous amplicon for real time pcr - (reply: 1)
  1029. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  1030. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  1031. assistance with PCR amplification please - (reply: 3)
  1032. I need an urgent help with q-pcr amplification plots - (reply: 11)
  1033. Problems with the Specificity of the Primers - (reply: 2)
  1034. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  1035. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  1036. Degenerate primers PCR problem, Please help! - (reply: 2)
  1037. Hotstart PCR and unspecific Amplification - (reply: 3)
  1038. Primer design help needed - (reply: 1)
  1039. Primers no longer work - (reply: 1)
  1040. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  1041. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  1042. RT PCR for known snps detection? - SNPs detection (reply: 1)
  1043. colony PCR - (reply: 2)
  1044. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  1045. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  1046. PCR reaction without template gives a product - (reply: 6)
  1047. PCR wrong product size - (reply: 6)
  1048. Wrong pcr product size - (reply: 4)
  1049. real time PCR machine - (reply: 5)
  1050. PCR very faint product - (reply: 1)
  1051. Question about principle of PCR - (reply: 3)
  1052. PCR amplification before bisulfite conversion - (reply: 6)
  1053. 4 question for real time PCR - (reply: 5)
  1054. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  1055. PCR didn't work - suspect the enzyme (reply: 2)
  1056. Find universal primers in vector - any online tool? (reply: 6)
  1057. Picking primers to confirm Illumina meth27 results - (reply: 2)
  1058. Colony PCR Specificity - (reply: 2)
  1059. Colony Pcr - primers - (reply: 5)
  1060. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  1061. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  1062. Primer Dilution Problem - D'oh! (reply: 2)
  1063. Using PCR to create overhangs - (reply: 3)
  1064. Creating overhangs with PCR - (reply: 4)
  1065. Problem with PCR on bacmids - (reply: 1)
  1066. Is there any software that can be sued to design degenerate primers - (reply: 1)
  1067. Running real time pcr product on gel?.. - (reply: 4)
  1068. Primer Decontamination - (reply: 2)
  1069. PCR mutagenesis of plasmid - (reply: 5)
  1070. tools for checking non-specific binding of primers -
    (reply: 1)
  1071. Primer designed for ARMS PCR - (reply: 2)
  1072. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  1073. methylation specific PCR - Primers - (reply: 1)
  1074. bacterial identification using real time PCR - (reply: 1)
  1075. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1076. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1077. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1078. colony PCR for subcloning gene - (reply: 1)
  1079. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1080. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1081. PCR reamplification - (reply: 2)
  1082. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1083. How many colonies to screen (colony PCR)? - (reply: 4)
  1084. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1085. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1086. question about pcr - (reply: 3)
  1087. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1088. How to construct a standard curve for real time PCR - (reply: 1)
  1089. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1090. PCR with one primer - (reply: 1)
  1091. colony PCR inconsistent - (reply: 4)
  1092. PCR contamination - (reply: 19)
  1093. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1094. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1095. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1096. PCR not working - No amplification - (reply: 5)
  1097. Quantification of PCR products - (reply: 1)
  1098. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1099. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1100. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1101. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1102. Nested PCR question - (reply: 1)
  1103. High Tm on primers - cannot get a product - (reply: 8)
  1104. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1105. undesired products in multiplex PCR - (reply: 3)
  1106. NCBI primer blast problem - (reply: 1)
  1107. Help with Multiplex Nested PCR - (reply: 2)
  1108. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1109. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1110. primer sequence problem - (reply: 2)
  1111. Finding bisulfite PCR primer sequence - (reply: 3)
  1112. PCR -No band formation - (reply: 1)
  1113. PCR - No Band formation - (reply: 3)
  1114. No amplification after bisulfite treatment - (reply: 4)
  1115. PCR AMPLIFICATION - (reply: 1)
  1116. Ligation of Blunt PCR Product - (reply: 1)
  1117. real time pcr need advise - (reply: 2)
  1118. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1119. PCR & Cloning - (reply: 6)
  1120. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1121. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1122. DNA pooling for PCR - Saving money PCR (reply: 7)
  1123. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1124. PCR need some help - (reply: 4)
  1125. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1126. Left polymerase out overnight - Will it still work? (reply: 1)
  1127. Primer Reconstitution--does temperature matter? - (reply: 5)
  1128. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1129. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1130. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1131. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1132. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1133. no PCR product from input... - (reply: 8)
  1134. MSP - unwanted amplification - (reply: 5)
  1135. Smears in Bisulfite seq. PCR - (reply: 3)
  1136. Primer design for qPCR - (reply: 2)
  1137. Primer design for qPCR - (reply: 1)
  1138. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1139. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1140. Multiple bands after bisulfite PCR - (reply: 7)
  1141. PCR genomic DNA of high GC content - (reply: 6)
  1142. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1143. primer design tips - (reply: 3)
  1144. PCR with Biotin Incorporation - (reply: 2)
  1145. pcr product - hello all (reply: 1)
  1146. design pcr primer - (reply: 1)
  1147. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1148. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1149. PCR with long and complex primers - (reply: 2)
  1150. Phospholylation of primers - (reply: 1)
  1151. apoptosis by PCR? - (reply: 12)
  1152. Re-using PCR plates? - (reply: 2)
  1153. Touchdown PCR issues - (reply: 3)
  1154. LINE1 OFR2 Primer Design - (reply: 1)
  1155. phosphorylation of primers using PNK - (reply: 1)
  1156. RT-PCR contamination issue - (reply: 3)
  1157. Primers for yeast (18S) - (reply: 1)
  1158. How to reduce the bisulfite PCR bias? - (reply: 4)
  1159. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1160. Primer Concentration to lower Ct - (reply: 1)
  1161. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1162. Primer Concentration Help - (reply: 4)
  1163. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1164. Primer Degradation - What exactly happens? (reply: 1)
  1165. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1166. PCR/DNA Extraction Problem - (reply: 5)
  1167. Primers in 2 exons - (reply: 2)
  1168. NTC appear in real time pcr - (reply: 6)
  1169. Problems with designing a primer - (reply: 2)
  1170. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1171. PCR of AT-rich DNA - (reply: 1)
  1172. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1173. Overlapping PCR - really need help ! - (reply: 5)
  1174. Freezing PCR product??? - (reply: 1)
  1175. problem with pcr cloning from mouse cDNA - (reply: 2)
  1176. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1177. PCR with Platinum Taq - product yield issues - (reply: 3)
  1178. real time PCR - (reply: 7)
  1179. Different sized product for same BS primer! - (reply: 2)
  1180. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1181. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1182. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1183. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1184. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1185. primers with restriction site - (reply: 1)
  1186. PCR product appear two close band in my gel - (reply: 15)
  1187. The Case of a Missing Band: PCR Issue - (reply: 4)
  1188. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1189. cDNA as PCR template - (reply: 1)
  1190. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1191. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1192. paranormal qpcr amplification activity - melting curves (reply: 3)
  1193. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1194. PCR template concentration - (reply: 6)
  1195. Bands in PCR negative control - (reply: 3)
  1196. PCR efficiency calculated by Linreg - (reply: 3)
  1197. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1198. primer design by generunner DG - (reply: 1)
  1199. degenerate pcr - (reply: 1)
  1200. BSP primers design, help please - (reply: 2)
  1201. primers with fluoresent dye PCR cycle question - (reply: 4)
  1202. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1203. Smears on PCR products - (reply: 4)
  1204. Dumb Reverse Transcription PCR Question - (reply: 2)
  1205. Reverse transcription PCR - (reply: 1)
  1206. PCR/RT-PCR beads - (reply: 2)
  1207. High Amplification Efficiency in Std. Curve - (reply: 5)
  1208. Non specific products in Bisulphite pcr - (reply: 4)
  1209. Run PCR amplifications in agarose gels - (reply: 3)
  1210. Problems with validating primers and low expression genes - (reply: 3)
  1211. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1212. Bisulfite sequencing PCR not working - (reply: 5)
  1213. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1214. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1215. intron/exon spanning primers - (reply: 1)
  1216. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1217. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1218. PCR product for sequencing - sample storage?? (reply: 2)
  1219. Absolute quantification using Real Time PCR - (reply: 2)
  1220. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1221. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1222. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1223. MSP primer troubles - (reply: 3)
  1224. Measuring global methylation using real time PCR - (reply: 2)
  1225. Designing Primers for multiple Isoforms - (reply: 1)
  1226. RT-PCR standard curve dilutions - (reply: 2)
  1227. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1228. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1229. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1230. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1231. PCR product dimer issue - (reply: 24)
  1232. PCR additives Formamide - Formamide which one? (reply: 4)
  1233. PCR Master mix - (reply: 1)
  1234. Addition of Restriction sites into PCR primers - (reply: 4)
  1235. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1236. failure PCR amplification from low GC content gene - (reply: 5)
  1237. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1238. conventional v real-time PCR applications - (reply: 1)
  1239. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1240. PCR reagents, can I use reagents from different manufacturers? - (reply: 6)
  1241. different PCR primers for real-time and classic PCR - (reply: 3)
  1242. primers deconamination??? - (reply: 6)
  1243. PCR band too thick - PCR troubleshooting question (reply: 5)
  1244. RT-PCR problem - (reply: 1)
  1245. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1246. primer check!!! - (reply: 6)
  1247. PCR bands on gel electrophoresis - (reply: 5)
  1248. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1249. long range PCR - (reply: 2)
  1250. Annealing Temperature of biotinylated primers - (reply: 2)
  1251. RT-PCR - New to RT-PCR info (reply: 2)
  1252. PCR punch - (reply: 4)
  1253. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1254. PCR off plasmid for screening - (reply: 2)
  1255. PCR failed no product.. help - (reply: 10)
  1256. number of molecules in PCR - (reply: 3)
  1257. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1258. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1259. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1260. Primer dilution - PCR primer dilution (reply: 2)
  1261. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1262. Can't re-PCR the PCR product - (reply: 5)
  1263. PCR reagent autoclave - (reply: 2)
  1264. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1265. Long Primers for PCR - (reply: 2)
  1266. Data Analysis for Real-time PCR - (reply: 2)
  1267. PCR not working - overhangs too long? (reply: 3)
  1268. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1269. argh more pcr headaches! - (reply: 6)
  1270. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1271. Chlamydia PCR cycling conditions - (reply: 1)
  1272. Primers going "loopy" - (reply: 2)
  1273. Conversion of primer unit - ug/ul to uM (reply: 4)
  1274. mutagenesis by PCR or just use a kit - (reply: 5)
  1275. Unsuccessful BS PCR - (reply: 3)
  1276. Primer Trouble Shooting - (reply: 5)
  1277. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1278. Smearing on gel in PCR products - (reply: 5)
  1279. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1280. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1281. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1282. Relative RT-PCR - (reply: 2)
  1283. Relative real time RT PCR - (reply: 2)
  1284. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1285. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1286. a simple primer problem - (reply: 5)
  1287. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1288. no or poor amplification - (reply: 1)
  1289. Primers from 3'UTR - (reply: 2)
  1290. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1291. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1292. Designing primers with ESTs - (reply: 2)
  1293. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1294. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1295. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1296. Real Time vs Traditional PCR results - (reply: 1)
  1297. The problems to identify mouse genotype with PCR - (reply: 1)
  1298. addition of BclI restriction site to PCR primer - (reply: 6)
  1299. non specific amplification - DNA amplification by PCR (reply: 1)
  1300. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1301. How does methprimer calculate primer Tm - (reply: 2)
  1302. Laminar Flow vs PCR cabinet - (reply: 4)
  1303. How to do PCR to detect mRNA without RT? - (reply: 2)
  1304. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1305. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1306. PCR has stopped working - (reply: 7)
  1307. PCR negative control contamination - (reply: 8)
  1308. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1309. Phosphorylated primers - (reply: 5)
  1310. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1311. PCR efficiency in real timePCR - (reply: 5)
  1312. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1313. Drastic Decrease in PCR product yield - (reply: 2)
  1314. resources on PCR principles & technique - (reply: 1)
  1315. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1316. Colony Screeing without using PCR - (reply: 8)
  1317. High MW PCR band seen...help needed - (reply: 7)
  1318. QPCR - Primer and Probe question - QPCR (reply: 2)
  1319. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1320. standard curve/ PCR efficiency problems... - (reply: 1)
  1321. Help! PCR that used to work doesn't work now! - (reply: 2)
  1322. PCR and templates - (reply: 3)
  1323. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1324. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1325. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1326. Easy primer question? - Primers (reply: 1)
  1327. PCR inconsistency - (reply: 4)
  1328. Plasmid problem - From PCR product (reply: 7)
  1329. help needed: PCR a gene from genomic DNA - (reply: 2)
  1330. questions about pcr products after pooling - (reply: 3)
  1331. stiching/linking/sewing pcr - (reply: 3)
  1332. Human mtDNA amplification problems - (reply: 3)
  1333. primer dimer - proplems (reply: 1)
  1334. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1335. Nested PCR - (reply: 2)
  1336. pfu vs long pcr mix - (reply: 7)
  1337. weird PCR ask for help - (reply: 12)
  1338. Methylation specific DMSO PCR - (reply: 1)
  1339. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1340. No PCR product at all - (reply: 8)
  1341. PCR troubleshoooooting - primer dimer (reply: 1)
  1342. Primer efficiency test - (reply: 1)
  1343. primer software - (reply: 2)
  1344. Question on wired PCR - (reply: 1)
  1345. restriction digestion of PCR product - (reply: 7)
  1346. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1347. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1348. How to know in which exon a primer match? - (reply: 1)
  1349. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1350. Amplification of human genomic DNA - (reply: 2)
  1351. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1352. sequencing with forward/reverse primers - (reply: 7)
  1353. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1354. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1355. Adding A overhangs - primer design implications? - (reply: 1)
  1356. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1357. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1358. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1359. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1360. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1361. primer design problem - (reply: 1)
  1362. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1363. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1364. Problem with primer efficiency analysis - (reply: 4)
  1365. slan real time PCR system for validation of microarray results? - (reply: 1)
  1366. PCR unusualband at ~230bps - PCR (reply: 1)
  1367. DMSO or BSA for PCR - (reply: 6)
  1368. Whole Genome Amplification - (reply: 2)
  1369. primer design - (reply: 1)
  1370. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1371. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1372. designing primers for genes not sequenced yet - (reply: 2)
  1373. Tris Buffer for PCR reaction - why? (reply: 1)
  1374. PCR a plasmid protein - (reply: 2)
  1375. multiplex PCR - (reply: 4)
  1376. Realtime PCR machine - (reply: 2)
  1377. PCR set-up calculation nightmares. - (reply: 3)
  1378. Primer desing - (reply: 2)
  1379. Colony PCR - (reply: 3)
  1380. Standard curves for PCR efficiency. - (reply: 2)
  1381. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1382. Question about RAPD PCR - (reply: 2)
  1383. Looking for help with my PCR! - (reply: 3)
  1384. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1385. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1386. primer design - (reply: 9)
  1387. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1388. PCR without thermal cycler? - (reply: 9)
  1389. primer contamination - primer contaminated with ice (reply: 3)
  1390. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1391. RT-PCR problem - (reply: 4)
  1392. Only DNA ladder , No desired band in PCR - (reply: 4)
  1393. taq and PCR - (reply: 6)
  1394. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1395. PCR Efficiency - (reply: 2)
  1396. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1397. Long PCR and genomic DNA isolation problems - (reply: 2)
  1398. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1399. NTC with specific amplification - (reply: 1)
  1400. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1401. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1402. Primer3 vs Primer BLAST - (reply: 3)
  1403. PCR and then ligation - (reply: 7)
  1404. Primer design: free energy - (reply: 2)
  1405. Amplification in NTC and noRT controls - (reply: 5)
  1406. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1407. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1408. asymmetric PCR - (reply: 1)
  1409. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1410. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1411. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1412. trouble with pcr - (reply: 8)
  1413. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1414. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1415. Real time PCR results-interpretation - (reply: 1)
  1416. Real time PCR results-interpretation - (reply: 2)
  1417. Primer optimization for ChIP - (reply: 2)
  1418. sequencing primer: T7 or T7promoter? - (reply: 4)
  1419. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1420. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1421. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1422. primer tm - tm calculation for tagged primers (reply: 2)
  1423. Reusing 96 well plates for PCR - (reply: 2)
  1424. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1425. PCR and AFLP - (reply: 1)
  1426. Universal 16s rRNA primers needed - (reply: 3)
  1427. Identifying PCR Inhibitors - (reply: 10)
  1428. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1429. PCR + phusion enzyme = massive errors - (reply: 4)
  1430. long DNA amplification - (reply: 11)
  1431. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1432. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1433. adding koazak sequence to primer - (reply: 1)
  1434. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1435. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1436. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1437. Primer design if sequence is unknown for organism - (reply: 5)
  1438. adding C terminal tag to reverse primer - (reply: 1)
  1439. epitope tagging of pcr products - (reply: 1)
  1440. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1441. Real time PCR info - (reply: 4)
  1442. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1443. PCR from BAC - PCR from BAC (reply: 1)
  1444. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1445. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1446. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1447. How much product PCR?? - (reply: 3)
  1448. what primers should I use for DNA sequencing? please help - (reply: 2)
  1449. what primers should I use for DNA sequencing?? - (reply: 3)
  1450. Is my primer going to work for qRT-PCR? - (reply: 6)
  1451. Standard curve for RT-PCR - (reply: 1)
  1452. PCR using genomic DNA - (reply: 2)
  1453. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1454. Problem amplifying plasmid - (reply: 1)
  1455. Sybr Green I in real-time PCR reaction - (reply: 3)
  1456. Primers annealing temperatures - (reply: 8)
  1457. Difficulties cloning by PCR - (reply: 5)
  1458. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1459. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1460. high Cp value PCR - (reply: 2)
  1461. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1462. Synthesize own Oligo-dT primers - (reply: 6)
  1463. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1464. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1465. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1466. Smearing/ no amplification in PCR - (reply: 3)
  1467. primer design - Primer design for PCR (reply: 1)
  1468. How are primers designed? - (reply: 4)
  1469. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1470. DNA methylated PCR - (reply: 2)
  1471. need help on primer calcuation soon - (reply: 1)
  1472. primer design - (reply: 6)
  1473. problem in pcr and purification - (reply: 3)
  1474. Inability to duplicate PCR results - (reply: 8)
  1475. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1476. PCR using xt5 - (reply: 2)
  1477. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1478. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1479. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1480. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1481. 23S rRNA Primer - need primer sequence (reply: 1)
  1482. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1483. RT-PCR - problems with TaqMan PCR (reply: 2)
  1484. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1485. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1486. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1487. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1488. Small product amplification problems - (reply: 4)
  1489. multi way ligation or long range pcr? - (reply: 1)
  1490. Freezing primers+SYBR green - (reply: 2)
  1491. Tagged Primers - (reply: 5)
  1492. no pcr amplification product - (reply: 9)
  1493. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1494. design primer for chip - (reply: 5)
  1495. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1496. PCR problem - (reply: 4)
  1497. differently behavioring replicates in real-time PCR - (reply: 1)
  1498. Multiplex PCR - (reply: 1)
  1499. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1500. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1501. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1502. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1503. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1504. PCR components storage - (reply: 3)
  1505. reconstitution of primers - (reply: 2)
  1506. No PCR bands with some templates - (reply: 5)
  1507. Mysterious PCR Contamination - (reply: 13)
  1508. BS PCR..please help - DNA methylation analysis (reply: 15)
  1509. RT-PCR Help - Protocol provided (reply: 4)
  1510. PCR product purification - (reply: 3)
  1511. Mutagenesis PCR and undesired amplification - (reply: 2)
  1512. Temp Gradient PCR - (reply: 2)
  1513. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1514. PCR with genomic dna - (reply: 9)
  1515. primer dimer - (reply: 2)
  1516. PCR with long primers - (reply: 3)
  1517. BSP PCR standardization...help - (reply: 8)
  1518. PCR on degraded templates - (reply: 2)
  1519. PCR help needed - (reply: 3)
  1520. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1521. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1522. light bands, dark smear, dark dimers - (reply: 2)
  1523. PCR, followed by sequencing... - why ?? (reply: 3)
  1524. PCR program - strange extension step (reply: 2)
  1525. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1526. Help me! How to design nested BSP primers? - (reply: 4)
  1527. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1528. Plasmid linearization by PCR - (reply: 4)
  1529. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1530. Using BLAST to check primers - (reply: 17)
  1531. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1532. pcr doesnt show any band for DNa walking - (reply: 8)
  1533. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1534. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1535. Effects of more cDNA in PCR??? - (reply: 1)
  1536. PCR primer - (reply: 2)
  1537. PCR product disappears after restriction digestion - (reply: 4)
  1538. Primer design - GC clamp - (reply: 8)
  1539. Real Time PCR - excluding Ct - (reply: 2)
  1540. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1541. Price of a real time PCR machine? - (reply: 4)
  1542. colony PCR - (reply: 8)
  1543. probe conc. of Asymmetric PCR - (reply: 1)
  1544. primer dimer or what? - (reply: 2)
  1545. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1546. Real Time PCR method comments - (reply: 2)
  1547. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1548. colony pcr - (reply: 4)
  1549. Primers and Taqman Probes mixture question - (reply: 1)
  1550. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1551. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1552. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1553. Colony PCR - (reply: 15)
  1554. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1555. no bands in my PCR - (reply: 9)
  1556. designing primers - (reply: 1)
  1557. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1558. purefication and amplification of serum free DNA - (reply: 1)
  1559. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1560. Bisulfite Sequencing PCR - (reply: 4)
  1561. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1562. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1563. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1564. mRNA PCR - (reply: 1)
  1565. pcr product quantification - (reply: 5)
  1566. Negative Control Primers for ChIP Assay - (reply: 3)
  1567. Magnesium in PCR - (reply: 1)
  1568. PCR Sample Prep - (reply: 4)
  1569. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1570. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1571. Bizarre Contamination in PCR - (reply: 7)
  1572. PCR Cloning-large primers - (reply: 4)
  1573. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1574. forward and reverse primer - (reply: 4)
  1575. Check the primers - (reply: 4)
  1576. PCR problem from transformed TOPO TA vector - (reply: 3)
  1577. DIG DNA PCR labeling problem - (reply: 6)
  1578. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1579. BSP primers - (reply: 2)
  1580. Various size of insert after colony screen by PCR - (reply: 2)
  1581. inverse PCR molecular bilogy - (reply: 3)
  1582. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1583. failed PCR on DNA extract from blood - (reply: 6)
  1584. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1585. Primer dimer issue in real time PCR - (reply: 21)
  1586. Leaky RT-PCR - (reply: 4)
  1587. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1588. PCR, RNA, Northern Blotting???? - (reply: 1)
  1589. bizzare PCR smear - why does science hate me (reply: 7)
  1590. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1591. PCR detection of SNP - (reply: 6)
  1592. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1593. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1594. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1595. Sub-cloning sticky-end PCR products - (reply: 2)
  1596. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1597. ChIP on chip amplification problems - (reply: 11)
  1598. Genotyping PCR - (reply: 3)
  1599. PCR screening of transformed bacterial colony - (reply: 6)
  1600. Is this standard valid? - pcr product as standards (reply: 5)
  1601. primer binding - (reply: 1)
  1602. weird ChIP primers! - (reply: 1)
  1603. PCR primers - (reply: 1)
  1604. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1605. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1606. amplification in negative control in RT PCR - (reply: 2)
  1607. miRNA real time PCR by SYBR green methods - (reply: 1)
  1608. Deletion PCR - (reply: 9)
  1609. diluting to final primer concentration - please help correct (reply: 1)
  1610. Smear problem with my new primer set - (reply: 2)
  1611. PCR ghost bands - (reply: 1)
  1612. Amplifying a gene using a degenerate primer - (reply: 4)
  1613. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1614. CP of real time PCR by LC480 - (reply: 1)
  1615. CHIP- Real Time PCR calculations - (reply: 1)
  1616. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1617. transgene copy number - using real time PCR (reply: 1)
  1618. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1619. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1620. Unspecific PCR - (reply: 7)
  1621. Real-Time PCR as a Microplate Reader - (reply: 1)
  1622. gene-specific RT-PCR - (reply: 4)
  1623. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1624. RT-PCR Internal Standard - (reply: 2)
  1625. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1626. Plasmid supercoiling affecting PCR? - (reply: 2)
  1627. RNA contamination in PCR - (reply: 1)
  1628. PCR without DNA extraction! - (reply: 2)
  1629. Primer tm - (reply: 1)
  1630. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1631. re-amplification in real-time PCR - (reply: 1)
  1632. primer design - really necessary? (reply: 2)
  1633. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1634. RT-PCR - (reply: 3)
  1635. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1636. Degenerative primers = multiple products? - (reply: 5)
  1637. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1638. PCR problems on a ligation product - (reply: 5)
  1639. Genomic DNA and PCR - (reply: 4)
  1640. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1641. Sewing PCR - (reply: 3)
  1642. on the minimal pcr derived product - (reply: 2)
  1643. primer dimers - (reply: 5)
  1644. RT-PCR vs. conventional PCR - (reply: 1)
  1645. qPCR primer design - (reply: 5)
  1646. PCR and gel purification problem - did I screw this up (reply: 4)
  1647. Cloning PCR fragment - (reply: 1)
  1648. touchdown pcr - (reply: 6)
  1649. non specific pcr products - (reply: 4)
  1650. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1651. Anyone with experiences in 2 step PCR - (reply: 1)
  1652. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1653. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1654. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1655. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1656. re-amplification of a PCR product - is it recommended? (reply: 7)
  1657. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1658. PCR machine with accurate temperature control? - (reply: 1)
  1659. Gene-specific RT-PCR - (reply: 2)
  1660. Primer stock confusion - (reply: 6)
  1661. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1662. primer design - primer design question (reply: 2)
  1663. Protocol for 16S rDNA real time PCR - (reply: 2)
  1664. Touchdown PCR - (reply: 1)
  1665. Determine annealing temperature of primers - (reply: 14)
  1666. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1667. polymerase for BSP - (reply: 2)
  1668. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1669. Validating real time pcr primers - (reply: 2)
  1670. BSP,MSP primer design? - (reply: 1)
  1671. low gc primers - help with pcr using low gc primers (reply: 6)
  1672. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1673. Primers and annealing temperature - (reply: 2)
  1674. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1675. Copy number calculations in real time PCR - (reply: 2)
  1676. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1677. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1678. RT-PCR Negative controls - (reply: 1)
  1679. WHich primer to use for sequencing - (reply: 5)
  1680. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1681. minimum length for the gene to be amplified in PCR - (reply: 6)
  1682. dNTP Quantity - (reply: 3)
  1683. annoying PCR cloning problem (season 2) - (reply: 5)
  1684. PCR with 60 bp primers - results in no product (reply: 6)
  1685. Amplification dwindles using little template RT-PCR - (reply: 3)
  1686. Running gel after RT-PCR - (reply: 5)
  1687. Degenerate PCR Size Limit? - (reply: 3)
  1688. primer design for pBAD topo expression kit - (reply: 2)
  1689. PCR problems.. - problems with conventional PCR (reply: 3)
  1690. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1691. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1692. small PCR products on agarose gel? - (reply: 8)
  1693. Dnase digestion and PCR - (reply: 4)
  1694. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1695. primers fpr sequencing - (reply: 4)
  1696. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1697. Need an UV260 RT-PCR instrument - (reply: 1)
  1698. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1699. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1700. PCR produces products of wrong size - (reply: 4)
  1701. PCR with pfu and degenerate primers (?) - (reply: 7)
  1702. a doubt about PCR gel purification - (reply: 4)
  1703. Draw PCR primer locations - PCR primer (reply: 4)
  1704. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1705. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1706. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1707. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1708. PCR+enhancer - (reply: 4)
  1709. Serious issues with RT-PCR - (reply: 7)
  1710. PCR [dNTP] - dNTP concentrations (reply: 2)
  1711. PCR issue - (reply: 2)
  1712. dNTP question - (reply: 3)
  1713. PCR - (reply: 1)
  1714. long PCR primer - (reply: 7)
  1715. Taqman rtPCR primer and probe design - (reply: 3)
  1716. sequencing problem after pcr - (reply: 4)
  1717. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1718. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1719. primer design - (reply: 3)
  1720. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1721. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1722. Analysis of ChIP RT-PCR data - (reply: 7)
  1723. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1724. Negative flouroscence in real time PCR - (reply: 2)
  1725. Minimum number of PCR cycles to see a product? - (reply: 6)
  1726. decontamination - decontamination for PCR (reply: 6)
  1727. Troubleshoot ARMS PCR - (reply: 2)
  1728. negative control for PCR - (reply: 1)
  1729. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1730. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1731. pcr pdt amplification - (reply: 1)
  1732. real time PCR - (reply: 3)
  1733. PCR fails when I scale up - (reply: 2)
  1734. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1735. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1736. PCR not Working = SMEAR - (reply: 3)
  1737. How to do PCR fragment in 2 steps - (reply: 3)
  1738. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1739. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1740. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1741. RT-PCR - problem of PCR (reply: 1)
  1742. PCR product longer than template - why? - (reply: 2)
  1743. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1744. Dye recipe that can be used for PCR MM - (reply: 3)
  1745. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1746. PCR conditions with three primers - (reply: 1)
  1747. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1748. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1749. Cloning a labeled PCR product - (reply: 1)
  1750. PCR and cloning - (reply: 14)
  1751. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1752. PCR product - not suppose to be there (reply: 7)
  1753. using the meth primer - (reply: 2)
  1754. Generating primers for MSP - (reply: 1)
  1755. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1756. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1757. PCR from a smear genomic DNA ? - (reply: 5)
  1758. Primer design and need help - (reply: 11)
  1759. Setting up a multiplex PCR assay. - (reply: 2)
  1760. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1761. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1762. cDNA and RT-PCR - (reply: 8)
  1763. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1764. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1765. PCR problem - help me plsss (reply: 9)
  1766. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1767. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1768. PCR efficiencies - (reply: 4)
  1769. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1770. PCR quantification - (reply: 1)
  1771. PCR quantification - (reply: 1)
  1772. Long PCR product - (reply: 9)
  1773. problem in Pcr amplification - (reply: 7)
  1774. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1775. difference between hotstart and taq polymerase - (reply: 7)
  1776. sequencing the pcr product - (reply: 15)
  1777. Pcr amplification - primer designed from UTR regions (reply: 5)
  1778. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)