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PCR Related Discussions
  1. -RT contamination problem in PCR - (reply: 4)
  2. qPCR std curve calculation issue if using PCR product as the std - (reply: 1)
  3. Thermal Cycler for Multiplex PCR - (reply: 3)
  4. Problem of melt curve shape for pikoreal real time PCR - (reply: 1)
  5. Primer sequences with M and Y - how to order? - (reply: 2)
  6. RT-PCR low efficiency - (reply: 1)
  7. emulsion PCR - (reply: 1)
  8. RT-PCR electrophorasis - (reply: 1)
  9. PCR contamination - (reply: 5)
  10. PCR bands on gel - (reply: 5)
  11. Problem with restriction digestion of cloned PCR product - (reply: 5)
  12. SYBR Green Real time PCR - (reply: 2)
  13. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
  14. need help calculating stock primer concentration - (reply: 1)
  15. High background fluorescence detected before starting PCR - (reply: 3)
  16. How to clean up PCR place & pipettes? - (reply: 1)
  17. Real-time pcr - (reply: 1)
  18. Using qPCR machine for simple PCR, is it possible? - (reply: 3)
  19. PCR inhibitors - (reply: 1)
  20. What could be the possible reasons for a RT-PCR experiment that was working fine - (reply: 4)
  21. Polymerase mixture for blunt ended fragments - (reply: 2)
  22. how to design PCR primer with a tag region which use for In-frame deletion gene? - (reply: 10)
  23. Amplifying shRNA lentivirus - (reply: 1)
  24. Mineral oil quality in PCR tubes - (reply: 5)
  25. Diagnostic PCR troubleshooting - (reply: 2)
  26. RT-PCR Kit's Validation - (reply: 1)
  27. Rt pcr - (reply: 4)
  28. Inhibition of PCR amplification of bacterial genomic DNA by RNA - (reply: 1)
  29. methylation specifc primers thermal cycle conditions - (reply: 4)
  30. PCR Amplification Issues and Primer dimer - (reply: 4)
  31. How to make PCR analysis using the 22DDCT Method - (reply: 1)
  32. Primer calculation for qPCR - final reaction - (reply: 1)
  33. Sec. structure in primers - (reply: 1)
  34. Changing PCR recipe halfway - (reply: 1)
  35. PCR master mix containing primers and Taq - (reply: 4)
  36. Primer working with some cDNA samples and not other - (reply: 2)
  37. no bands after pcr!!!! - (reply: 4)
  38. Experimental design for RT-PCR - (reply: 8)
  39. Primer dilution - (reply: 7)
  40. why not working msp primers - (reply: 4)
  41. PCR product not migrating from wells...?? - (reply: 6)
  42. Re-PCR my PCR products - (reply: 2)
  43. PCR DIG-labeling kit preblem? - (reply: 1)
  44. pcr - (reply: 1)
  45. how to analysis the BiSearch ePCR result - (reply: 3)
  46. Standardizing cDNA concentration before doing PCR - (reply: 4)
  47. Volume of Primers - (reply: 4)
  48. PCR conditions unknown? - (reply: 10)
  49. PCR anomaly in a mid-range dilution of the template - (reply: 2)
  50. Reducing contamination in 16s PCR for metagenomic library prer - (reply: 4)
  51. MSP_Primer dimers? - (reply: 17)
  52. PCR using very long oligos !!! - (reply: 4)
  53. Primer Designing - (reply: 9)
  54. Housekeeping gene for Real Time PCR when comparing expression of genes across di - (reply: 1)
  55. Colour of PCR gel band best for ImageJ analysis - (reply: 2)
  56. colony pcr, two strong bands - (reply: 4)
  57. Ligation of two PCR products - (reply: 1)
  58. pcr primer design - (reply: 6)
  59. Please help (primer/ probe final volume calculations) - (reply: 1)
  60. Primer design with EcoRI ends - (reply: 2)
  61. Amplification problem in DNA isolated after ChIP experiment, - (reply: 1)
  62. Trouble with PCR using ligation mix as template? - (reply: 3)
  63. PCR with mammalian cells as template - (reply: 1)
  64. PCR with overlapping primers only (primer extention) - (reply: 3)
  65. Random chuck of nonsense in middle of PCR - (reply: 6)
  66. RT-PCR analysis - (reply: 1)
  67. PCR DNA bands too large on agarose gel - (reply: 1)
  68. MSP Primer design - (reply: 5)
  69. New to PCR - (reply: 3)
  70. How can I check the quality of bacterial primers and their specificity on blast? - (reply: 1)
  71. 3-primer PCR Genotyping - problem in hemizygotes - (reply: 2)
  72. Primer design - (reply: 1)
  73. What % of colon cancer patients does a general colon PCR array detect - (reply: 1)
  74. Primer non specific binding sites - (reply: 7)
  75. Degenerate primers - (reply: 8)
  76. Primers with 2 different annealing temp - (reply: 1)
  77. Can you use same samples for real time pcr 2nd time? - (reply: 2)
  78. Staphylococcus aureus colony PCR - (reply: 1)
  79. Cloning purified PCR product into cells - (reply: 5)
  80. PCR & Western Blot sample preperation - (reply: 2)
  81. PCR & Western Blot sample preperation - (reply: 5)
  82. Y-linker transgene integration site PCR primer verification - (reply: 1)
  83. Non especific band in PCR... - (reply: 2)
  84. I need help with PCR problems - (reply: 5)
  85. Question about baselines in Real-Time PCR - (reply: 3)
  86. Inconsistent colony PCR result - (reply: 2)
  87. A problem with PCR array - (reply: 4)
  88. My PCR suddenly stopped working and I'm losing my mind - (reply: 3)
  89. Concentration of Eva green with different amplicon sizes in a PCR pool - (reply: 1)
  90. labX offers on realtime PCR machines - (reply: 4)
  91. Could any of you help with an explanation to these vague real time PCR curves &# - (reply: 2)
  92. polymerase with or without proofreading activity used in study nucleotide polymo - (reply: 1)
  93. how to calculate amount of cDNA using RT-PCR - (reply: 2)
  94. Suggest best micropipette for PCR purpose... - (reply: 1)
  95. NEB TM CALCULATOR PRIMER CONCENTRATION - (reply: 2)
  96. Plasmid Amplification Issue - (reply: 6)
  97. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  98. primer design for a gene - (reply: 3)
  99. large bands in all pcr reactions including negative control - (reply: 1)
  100. confirmation for primers dilution - (reply: 10)
  101. Problems regarding amplification of my gene - (reply: 3)
  102. PCR working, qPCR is *not*. - (reply: 6)
  103. DIY PCR Cleanup/Gel extraction and miniprep solutions - (reply: 2)
  104. Overlapping PCR, need help - (reply: 5)
  105. How does RT-PCR work? - (reply: 5)
  106. Denatured plasmid for pcr - (reply: 1)
  107. Possible primer dimer problem..Need solution!!! - (reply: 2)
  108. confusing with where to put primers and which ordination - (reply: 4)
  109. Help with primer concentration for sequencing - (reply: 1)
  110. PCR troubleshoot - (reply: 6)
  111. Some info. on PCR products stability w/respect to shipping - (reply: 3)
  112. PCR Products not run well in the GEL - (reply: 1)
  113. PCR solution calculus - (reply: 4)
  114. Amplifying from pcr products - (reply: 7)
  115. Consistancy issue with PCR block - (reply: 1)
  116. Queries regarding PCR - (reply: 3)
  117. Design the primers - (reply: 10)
  118. Calculation of Taq Polymerase - (reply: 3)
  119. Best Taq for colony PCR? - (reply: 5)
  120. How two fragments joint together when doing fusion PCR? - (reply: 4)
  121. How to test my milleq water in q PCR about contaminants - (reply: 3)
  122. Mutagenesis PCR - (reply: 9)
  123. real time PCR primer design - (reply: 1)
  124. The PCR gods are frowning upon me - (reply: 9)
  125. PCR after T4 ligation? - (reply: 3)
  126. primers for cloning - (reply: 1)
  127. Degenerated Primers and their problems - (reply: 1)
  128. Sonication of small PCR amplicon - (reply: 1)
  129. primer design with restriction enzyme - (reply: 1)
  130. Interpreting primer BLAST scores for self-complementary - (reply: 5)
  131. primer dilution help - (reply: 1)
  132. Testing primers on 'unknown' tissue - (reply: 1)
  133. How to remove inhibitory substances in PCR? - (reply: 2)
  134. need some advise about PCR of PKD1-human gene - (reply: 7)
  135. puzzled with PCR outcome after BS treatment - (reply: 2)
  136. nested PCR for low viral load- HBV patient sample - (reply: 2)
  137. troubleshooting stubborn PCR - (reply: 6)
  138. Measuring pcr fragments in a gel - (reply: 1)
  139. Amplification in HK gene but not for target gene - (reply: 3)
  140. could you help me with my stem loop RT primer? - (reply: 1)
  141. What is the difference between Hot start polymerase and the taq polymerase - (reply: 3)
  142. How long can I store at - 30°C my PCR mix? - (reply: 6)
  143. How to set up real-time PCR for yes/no bands (rearrangement) - (reply: 4)
  144. RT-PCR help - (reply: 2)
  145. Autoclaving PCR waste in the room where PCRs are set up and run - Is it a proble - (reply: 2)
  146. PCR Temperature change control malfunctioning - (reply: 1)
  147. excess amount of primers - (reply: 2)
  148. Dehydrating and Reconstituting primers - (reply: 15)
  149. Trouble with PCR of short sequence - (reply: 3)
  150. Setting the temperature range for gradient PCR - (reply: 3)
  151. KIR gene promoters for MSP primers design - (reply: 2)
  152. Biotin-streptavidin signal amplification - (reply: 2)
  153. Any online tool to check the propability of primer dimer formetion _ - (reply: 4)
  154. Amplification curve in the negative control samples - (reply: 7)
  155. PCR of bisulfite converted DNA is now producing a smear? Previously produced a s - (reply: 2)
  156. PCR troubleshooting: wrong amplicon size when spiked into sample - (reply: 4)
  157. Electrophoresis after PCR : too many bands - (reply: 6)
  158. Troubles with PCR (Rosa26 locus in mouse line) VERY CONFUSING! - (reply: 6)
  159. Primer as limiting reagent in PCR reaction - (reply: 2)
  160. Primer Design for RNA probes - (reply: 2)
  161. Real time PCR for degraded RNA - (reply: 1)
  162. Microfluidic PCR Issues, Master Mix and Consumable Variations? - (reply: 5)
  163. Doubled polymerase in the rxn, does this increase pcr efficiency? - (reply: 1)
  164. If I am running Real-Time PCR with one gene of interest and one reference gene(G - (reply: 4)
  165. Do I need to run HKG together with the GOI everytime I run Real-Time PCR? - (reply: 1)
  166. Nested BSP Primer Design - (reply: 6)
  167. website for primer design - (reply: 1)
  168. PCR Efficiency over 150%! - (reply: 1)
  169. problem of amplification - (reply: 2)
  170. PCR not working - (reply: 11)
  171. How to do a primer dilution - (reply: 10)
  172. Troubles with Fusion PCR - (reply: 1)
  173. Primer design and alternative transcripts - (reply: 2)
  174. PCR and sequencing of genomic DNA - (reply: 5)
  175. qPCR amplification - (reply: 4)
  176. NESTED PCR - (reply: 6)
  177. Extremely desperate noob question: How do these PCR work? - (reply: 6)
  178. RT-PCR carry over contamination and dUTP/UDG - (reply: 4)
  179. problems regarding amplifying a 1.7 kb mRNA seq - (reply: 3)
  180. Digestion necessary after PCR? - (reply: 9)
  181. Inverse PCR product selection - (reply: 2)
  182. RT-PCR - High Ct Values and Laser Capture - (reply: 1)
  183. PCR with no bands showing in 1.1 % gel electrophoresis - (reply: 4)
  184. how to hasten real-time PCR amplifications - (reply: 2)
  185. Please please help me with my Phusion PCR. - (reply: 5)
  186. PCR - (reply: 1)
  187. NCBI Primer Design - Stringency Issues - (reply: 3)
  188. Different primer concentration in qPCR - (reply: 1)
  189. Multiple melt curves for primer in a sample that does not express gene - (reply: 6)
  190. help in long pcr - (reply: 1)
  191. PCR inhibitor in template DNA - (reply: 3)
  192. Why when disigning primers for RTPCR, you need to chose a region where introns a - (reply: 1)
  193. Problem with Real-time PCR results analysis - (reply: 1)
  194. Primer Specificity: Testing only one primer - (reply: 4)
  195. PCR from protozoa DNA - (reply: 3)
  196. Ligation-mediated PCR: Vent Polymerase, Why? - (reply: 7)
  197. tool for comparing many primers pairs - (reply: 4)
  198. PCR that leads to protein synthesis - (reply: 18)
  199. Real-time PCR does not work, while the conventional PCR (with same conditions an - (reply: 3)
  200. Use of DMSO in General PCR - (reply: 1)
  201. PCR product size confusion - (reply: 3)
  202. Pcr primers - (reply: 7)
  203. Concentration specification in PCR - (reply: 3)
  204. Guanidine isothiocyanate in PCR - (reply: 1)
  205. Primers have worked well but now getting primer dimers? - (reply: 2)
  206. I cannot design primers on exon-exon junction - (reply: 2)
  207. DNA Quantification of PCR Products - (reply: 2)
  208. Reverse transcribing GOI mRNA to cDNA for cloning? Gene specific primer or Poly - (reply: 4)
  209. Problem for PCR - (reply: 9)
  210. Designing primers in UTRs - (reply: 1)
  211. Multiplex PCR - (reply: 1)
  212. Whole mtDNA genome amplification with long-range PCR...trouble - (reply: 7)
  213. primer design@ buy? - (reply: 2)
  214. Trouble with overlap extension pcr - (reply: 3)
  215. who else can help me check primer. im a little bit less confident :(( - (reply: 9)
  216. designing primers( selecting target sequence/amplicon design) - (reply: 3)
  217. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  218. Question about the RT PCR - (reply: 3)
  219. faint dna band after pcr purification - (reply: 1)
  220. PCR ready mixes with long shelf lives - (reply: 4)
  221. Troubleshooting: Inverse PCR - (reply: 3)
  222. How to design primer to amplify genomic DNA? - (reply: 3)
  223. Overlap PCR, need help - (reply: 11)
  224. Internal control for miRNA RT-PCR - (reply: 1)
  225. Primers - (reply: 1)
  226. Primers mix - (reply: 2)
  227. question about RNA concentration for real time PCR - (reply: 1)
  228. Need help with dCAPS pcr, seeing huge bands on gel - (reply: 1)
  229. PCR products sizes and DNA ladder - (reply: 7)
  230. Having problem with primers for qPCR - (reply: 4)
  231. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  232. PCR product sequencing - (reply: 3)
  233. Bad fragment amplification - (reply: 4)
  234. Can somebody explain to me what "spiking" means in RT-PCR and why do you - (reply: 3)
  235. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  236. No amplification with TRAPEZE kit! - (reply: 1)
  237. PCR Purification or Gel Extraction for Southern Blot - (reply: 5)
  238. Stargazer PCR problems - (reply: 3)
  239. General PCR discussion - (reply: 8)
  240. Real time. No amplification but hight flourescence - (reply: 1)
  241. Real time PCR doubt - (reply: 5)
  242. PCR Profile for ligation - (reply: 3)
  243. protocol to relieve melanin inhibition of PCR - (reply: 4)
  244. No bands despite different primers and conditions and TAqs - (reply: 8)
  245. Multiplex TaqMan-like Assay PCR Efficiency - (reply: 3)
  246. Equimolar Mix Primer - (reply: 8)
  247. Wrong PCR product - (reply: 2)
  248. Colony PCR positive and Digestion negative????? - (reply: 11)
  249. Primer design - (reply: 5)
  250. storage for lyophilized primers - (reply: 1)
  251. PCR GAPDH gene - (reply: 1)
  252. Repeated mutagensis primer in site-directed mutagenesis - (reply: 8)
  253. Quantitative RT-PCR statistics help - (reply: 1)
  254. Dimerization of PCR product - (reply: 4)
  255. PCR Primer trouble - (reply: 2)
  256. Question about taq polymerase for multiplex PCR prior to NGS using Illumina tech - (reply: 1)
  257. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  258. is it necessary to introduce mismatches in the inner primers of tetra primer ARM - (reply: 1)
  259. finding the corrosponding primers - (reply: 2)
  260. Gene sequence for Real time PCR - (reply: 2)
  261. non-reproducible PCR results with cDNA as template - (reply: 1)
  262. Ligation of PCR fragments - (reply: 11)
  263. Information about the use of DNA diluted in Real Time PCR - (reply: 6)
  264. RNA extraction for RT-PCR - (reply: 3)
  265. PCR failing for transformed plant DNA but not for Agro - (reply: 3)
  266. problems with gDNA doing real time PCR in yeast - (reply: 2)
  267. Design PCR primers for cloning 3 copies of same insert - (reply: 2)
  268. Designing primers for a nuclear region to amplify in a species that has no nucle - (reply: 1)
  269. How to get rid of bands in PCR negative control - (reply: 10)
  270. T7 and M13 primers two band amplification - (reply: 3)
  271. Protocol for qPCR using the ABI SYBR® Green PCR Master Mix - (reply: 1)
  272. Strange PCR problem - (reply: 2)
  273. Question about Double Digestion followed by PCR amplification - (reply: 2)
  274. PCR problem - (reply: 2)
  275. PCR machine not working properly - (reply: 1)
  276. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  277. PCR gene specific amplification problem - (reply: 3)
  278. Failure SYBRGREEN PCR - (reply: 4)
  279. ***In Silico PCR producing output for incorrect sequence*** - (reply: 2)
  280. without RE site in PCR product - (reply: 5)
  281. Chicken (Gallus gallus) ITS-2 primers - (reply: 1)
  282. What do the values for ANY , SELF repersent when designing a primer in primer 3 - (reply: 1)
  283. real-time pcr non reproducible - (reply: 4)
  284. Strange amplification plots with high Ct variability - (reply: 1)
  285. How big a role does mixing play in PCR - (reply: 1)
  286. Melting curve is irregular for primer optimization - (reply: 5)
  287. Designing primers for ABO blood groups - (reply: 1)
  288. a smeared Gen-DNA template ---> smear and less yield on pcr ? - (reply: 1)
  289. RT-PCR primer design - (reply: 7)
  290. How to amplify very short PCR template - (reply: 4)
  291. Is this primer okay? - (reply: 4)
  292. PCR amplification of large template - (reply: 1)
  293. PCR insert - in frame? - (reply: 2)
  294. How to avoid nonspecific amplification (band) in PCR - (reply: 5)
  295. smear in gel electrophoresis after PCR - (reply: 2)
  296. Can I use Primer as template? - (reply: 1)
  297. Whole plasmid amplification by PCR - (reply: 2)
  298. PCR product as standard curve template - (reply: 6)
  299. colony PCR after transformation - (reply: 1)
  300. PCR machine - (reply: 3)
  301. qPCR - no amplification curve but suitable melting curve - (reply: 3)
  302. Designing a PCR based detection method for an unknown virus/pathogen - (reply: 2)
  303. Difficult PCR containing triplet repeats and Frt sites - (reply: 1)
  304. Resuspending primers calculation - (reply: 4)
  305. PCR of GC-rich sequence (E-cadherin) - (reply: 6)
  306. PCR RFLP is PCR product purification necessary? - (reply: 3)
  307. Mystery in my PCR - (reply: 5)
  308. Whole genome amplification from cDNA? - (reply: 4)
  309. What do you use to check primer secondary structure? - (reply: 3)
  310. Need help with my real time RT-PCR Plate Set up - (reply: 8)
  311. PCR product as template for in vitro transcription - (reply: 1)
  312. Problem with 3 step PCR - (reply: 3)
  313. mutagenic primers with very high GC content. - (reply: 3)
  314. Scaling up PCR to get more DNA - (reply: 5)
  315. PCR to get 10kbp product - (reply: 4)
  316. site-directed mutagenesis primer Tm - (reply: 4)
  317. Single-step nested PCR: how to investigate dynamics? - (reply: 2)
  318. Random vs oligo primer in preamplification RT - (reply: 1)
  319. how does polymerase stop at the required length - (reply: 1)
  320. Chip pcr. are there inespecifics? - (reply: 1)
  321. Primer concentration - stupid question - (reply: 3)
  322. Primer Design, help i´m New - (reply: 4)
  323. No DNA after PCR product purification - (reply: 9)
  324. Sensitivity of RT-PCR and qPCR - (reply: 4)
  325. RT-qPCR primer problem - (reply: 4)
  326. Mixing two cDNA samples into one for realtime PCR - (reply: 3)
  327. real time PCR trouble - (reply: 3)
  328. removal of ethanol in PCR product purification - (reply: 5)
  329. Crazy real time PCR curve - (reply: 4)
  330. [Video] Using NCBI for RT-PCR Primer Design - (reply: 1)
  331. High fidelity PCR trouble shooting - (reply: 2)
  332. Designing cloning primers for DNMT - (reply: 2)
  333. Reproducible Non-Specific PCR Product - (reply: 2)
  334. Are these primer products good enough for qPCR? - (reply: 3)
  335. Can't get PCR with large overhang primers to work - (reply: 8)
  336. Primer Efficiency across runs - (reply: 1)
  337. Question about pipet tips for PCR and rtPCR - (reply: 4)
  338. UNG in PCR - (reply: 1)
  339. Cause of random samples failing PCR? - (reply: 2)
  340. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  341. pcr 10X buffer preparation - (reply: 2)
  342. how much template do i need for pcr? - (reply: 5)
  343. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  344. Problems with crossover PCR - (reply: 2)
  345. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  346. Designing PCR Primers for cloning - (reply: 17)
  347. Creating primers to add restriction sites to vector backbone - (reply: 7)
  348. PCR DNA Concentration - (reply: 1)
  349. RT-PCR product- no band - (reply: 4)
  350. Some primers dose not work - (reply: 5)
  351. Understanding RACE PCR - (reply: 1)
  352. Pair of primers (SYBR) with more than one amplicon product(?) - (reply: 4)
  353. Wierd Bands after PCR....Confused - (reply: 9)
  354. Query regarding primers for quick change mutagenesis - (reply: 3)
  355. PCR with Plasmid recovered from filter paper - (reply: 6)
  356. PCR amplified product size - (reply: 5)
  357. the storage time for primers - (reply: 9)
  358. Annealing temperature for PCR - (reply: 8)
  359. can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR - (reply: 1)
  360. Nested PCR - (reply: 2)
  361. random primers or oligodT - (reply: 4)
  362. No amplification during RT-PCR - (reply: 4)
  363. Sequencing RT-PCR product - (reply: 3)
  364. Low yield PCR product after gel purification - (reply: 8)
  365. To design or use published primers? - (reply: 4)
  366. Using RT-PCR to find the presence of a deletion in a gene - (reply: 2)
  367. PCR primer usage (Clonning & cDNA) - (reply: 2)
  368. problem in cloning PCR primer design with restriction site - (reply: 4)
  369. Amplification with U primers, but not with M primers - (reply: 1)
  370. Taq polymerase - (reply: 7)
  371. His tag introduction in to gene and primer design - (reply: 2)
  372. the mechanism for microorganism replicating their genome without primer in vivo - (reply: 1)
  373. Which High-Fidelity polymerase is better? - (reply: 2)
  374. polymerase to use for cloning - (reply: 4)
  375. Problem amplifying viral gene - (reply: 5)
  376. Adding tag using overlapping PCR - (reply: 2)
  377. Tool / software for oligo analysis (hairpins, dimers etc.)? - (reply: 5)
  378. Design primer from incomplete sequence... - (reply: 2)
  379. Design primer from incomplete sequence... - (reply: 2)
  380. How to perform colony PCR - (reply: 1)
  381. Primer designing for Methylation - (reply: 6)
  382. Best way to isolate viral mRNA for RT-PCR? - (reply: 4)
  383. cDNA amplification problem - (reply: 4)
  384. Help me out with Primer calculation for point mutagenesis.... - (reply: 8)
  385. Gene expression from whole pancreas or islets by using RT-PCR - (reply: 2)
  386. PCR product running on agarose gel - (reply: 32)
  387. 5'end of pcr product was degraded after ligation and transformation - (reply: 5)
  388. No bands in PCR after DNase treatment - (reply: 4)
  389. PCR amplification with high fidelity enzyme - (reply: 1)
  390. PCR HELP!!!! - (reply: 1)
  391. Overlap PCR problem - (reply: 5)
  392. PCR and restriction enzyme digestion - (reply: 3)
  393. Bisulfite PCR and cloning - (reply: 5)
  394. PCR product purification - (reply: 4)
  395. Overlapping sequence PCR primers - (reply: 1)
  396. restricted PCR plasmid runs slower - (reply: 2)
  397. BSP PCR primer design explained - (reply: 11)
  398. Bisulfite sequencing PCR worked - (reply: 5)
  399. how to make a working solution of a primers for pcr reaction?? - (reply: 4)
  400. Whole lab experiencing inconsistent PCR contamination - (reply: 10)
  401. Stuck from last august! need help urgently - cloning and pcr ! - (reply: 7)
  402. doing PCR using PCR product , help plz !! - (reply: 5)
  403. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  404. good 16S univ. primer for qPCR? - (reply: 3)
  405. Strange looking "elevated" amplification plots from ChIP-qPCR samples - (reply: 2)
  406. Amplification of CAG promoter problems - (reply: 4)
  407. Can I use Real-time PCR instead of Southern Blot to determine number of integran - (reply: 1)
  408. Long PCR (>10kb) polymerase recommendations - (reply: 3)
  409. I need help for designing a pair of primers for this sequence. - (reply: 1)
  410. PCR not working for 5 Aza treated DNA - (reply: 3)
  411. Differentiating mouse from human cells with PCR - (reply: 5)
  412. Differentiate between RT-PCR and PCR - (reply: 3)
  413. PCR band slightly BELOW expected length ?! - (reply: 14)
  414. long primers PCR - (reply: 4)
  415. unexpected band in PCR with plasmid - (reply: 4)
  416. qPCR [template] and [primer] troubleshooting...... - (reply: 1)
  417. DNA polymerase for GC rich template? - (reply: 5)
  418. Sequencing Primers and Plasmids - (reply: 3)
  419. Good Protocol for Semi-Quantitative RT-PCR? - (reply: 1)
  420. DNA extraction - PCR Problem - (reply: 3)
  421. Primers to amplify Kan operon from pet28 - (reply: 2)
  422. PCR product biotinylation - (reply: 3)
  423. Tagged primers and a second PCR targetting the tags - (reply: 5)
  424. cloning pcr insert into plasmid - (reply: 2)
  425. Barcode generator for PCR primers - (reply: 2)
  426. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  427. Barcoding PCR products - (reply: 18)
  428. Biotinylated primers - (reply: 4)
  429. role of water in PCR - (reply: 3)
  430. HIV gene- PCR - (reply: 3)
  431. Real time PCR melting curve - (reply: 2)
  432. BSA in PCR reaction - (reply: 1)
  433. Transcription of PCR products without a primer coupled with promotor sequence - (reply: 4)
  434. Cannot see insert with colony pcr - (reply: 6)
  435. Amplicon as Template in PCR for TOPO TA Cloning - (reply: 10)
  436. isolating DNA from mouse tails and subsequent problems with pcr - (reply: 7)
  437. How to Join two PCR products - (reply: 3)
  438. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  439. What's wrong with my PCR - (reply: 5)
  440. Tagging a gene with HA and FLAG using PCR - (reply: 1)
  441. How do you screen promoter regions if you can only PCR a few thousand bp - (reply: 1)
  442. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  443. Problem with UPL real-time PCR - please help !!! - (reply: 1)
  444. Cp, but no amplification - (reply: 2)
  445. pcr product digestion problem - (reply: 4)
  446. Low PCR product - (reply: 7)
  447. Control primers for bisulfite-converted DNA - (reply: 3)
  448. Colony PCR - M13 vs. gene-specific primer amplification of bacmid - (reply: 4)
  449. DNA amplification differences between samples & between regions (WGA & s - (reply: 2)
  450. wrong dntp concentration - (reply: 2)
  451. Third round PCR - (reply: 2)
  452. long fragment PCR - (reply: 2)
  453. Best proof-reading polymerase? - (reply: 13)
  454. Is 27F and Anti-Gamma Primer the same thing for 16s? - (reply: 9)
  455. Q regarding primer mix for MSP!! - (reply: 4)
  456. Genomic DNA extraction - how to quantify for PCR? - (reply: 9)
  457. Inconsistent sample quality in quantitative real-time PCR - What could be the pr - (reply: 3)
  458. I need to break this cycle of PCR issues - (reply: 13)
  459. Questions / Help with Fusion PCR - (reply: 8)
  460. Controls for RT-PCR reactions - (reply: 2)
  461. Primer for a gene to create sticky ends and ligate an gene into a vector - (reply: 2)
  462. BSP Primers with M13 Tails - (reply: 1)
  463. Detection of T7 RNA polymerase by WB - (reply: 6)
  464. Multiplex PCR - (reply: 33)
  465. issues when designing PCR primers using PRIMER3 - (reply: 8)
  466. Primer catalog/databasing - (reply: 1)
  467. Nonrepetitive nested PCR - (reply: 2)
  468. Primer Blast - (reply: 6)
  469. Help designing primers for use in In-fushion cloning - (reply: 4)
  470. PCR internal control - (reply: 2)
  471. Primers Freeze-Thaw - (reply: 16)
  472. TDNA Genotyping PCR program - (reply: 1)
  473. primer design using 16s rRNA - (reply: 1)
  474. question about PCR and cloning - (reply: 1)
  475. PCR - (reply: 2)
  476. nonspecific band at 50bp in PCR - (reply: 9)
  477. Primer concentration for cDNA synthesis using GSP - (reply: 1)
  478. PCR master mix - (reply: 7)
  479. Maximum PCR product Tm - (reply: 6)
  480. what is 0.5 unit of Taq Polymerase? - (reply: 3)
  481. cloning from cDNA but got a much shorter PCR product - (reply: 4)
  482. PCR & Southern blotting - (reply: 1)
  483. Bisulfite PCR Question - (reply: 2)
  484. Primer Design HELP! - (reply: 3)
  485. Help with sequencing of a 120bp PCR product. - (reply: 9)
  486. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  487. How are Primers made? - (reply: 9)
  488. Long Non-Specific PCR Products - (reply: 5)
  489. Molecular plasmid amplification - Liquid preculture vs. overnight culture?? - (reply: 1)
  490. qPCR, Appearance of amplification curve for my minus RT control - (reply: 6)
  491. Use RT-PCR to verify after transient transfection a plasmid? - (reply: 5)
  492. PCR calculation - (reply: 1)
  493. Colony PCR and digestion with KpnI - (reply: 10)
  494. designing methylation specific primers - (reply: 3)
  495. Help with PCR and Gel Electrophoresis. Not getting any bands - (reply: 8)
  496. PCR - consistent false positive results - (reply: 6)
  497. PCR no amplification - (reply: 15)
  498. How to quantify virus by PCR - (reply: 12)
  499. Can human 18S primer, probe set for qPCR be used for Rhesus and Cynomolgus sampl - (reply: 3)
  500. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  501. Pcr product size determination for a fusion gene - (reply: 8)
  502. Quantify DNA before ChIP PCR - (reply: 3)
  503. Primer Annealing Temperatures - (reply: 24)
  504. my pcr product is larger than expected - (reply: 4)
  505. Question about PCR - DNA or RNA as template - (reply: 4)
  506. pcr purification - (reply: 1)
  507. pcr purification doubt - (reply: 4)
  508. improving sensitivity of one step TAQMAN REal time PCR - (reply: 1)
  509. PCR band moves lower than its size on agarose gel - (reply: 3)
  510. same primers, different product in conventional PCR and qPCR - (reply: 6)
  511. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  512. pcr problem - (reply: 6)
  513. NEB Q5 polymerase works very well - (reply: 1)
  514. Primers amplify genomic DNA but not cDNA - (reply: 2)
  515. pcr purification - (reply: 1)
  516. storing PCR product overnight - (reply: 2)
  517. Primer BLAST - (reply: 1)
  518. Bands in negative control PCR - (reply: 2)
  519. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  520. problem with pcr mutagenesis - (reply: 10)
  521. HMK-Tagged PCR Cloning Problem... - (reply: 4)
  522. Use PCR purification kit to purify restriction digestion - (reply: 2)
  523. PCR primer design - published primers trustable? - (reply: 8)
  524. Polymerase for E. coli screenings - (reply: 5)
  525. complicated RT-PCR issues - (reply: 4)
  526. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  527. Can we use degenerate bases in BSP primers? - (reply: 1)
  528. Quick change mutagenesis No PCR product visible - (reply: 2)
  529. Normalization of Real time PCR results using Pfaffl method - (reply: 2)
  530. RT-PCR vs plate reader for pathogen detection - (reply: 3)
  531. Can anyone help with my Bisulfite PCR? - (reply: 2)
  532. How to check primer sequences using BLAST and other tools? - (reply: 2)
  533. real time PCR analysis in patient samples - (reply: 2)
  534. carpet in gels for PCR products - (reply: 2)
  535. Primer Check? - (reply: 2)
  536. Sequencing of the PCR production in BSP - (reply: 1)
  537. amplification of 14Kb - (reply: 1)
  538. Validation of PCR primers/ probes - (reply: 6)
  539. Taq polymerase for MSP - (reply: 1)
  540. Real time PCR sudenly not working - (reply: 1)
  541. Buffer-composition One-Step RT-PCR - (reply: 4)
  542. Two reverse primer sequences for a single forward primer - (reply: 1)
  543. PCR with more than one primer - (reply: 1)
  544. RAPD - PCR problem - (reply: 5)
  545. Primer for identification of yeast (ITS or 18Sr RNA gene) - (reply: 3)
  546. How to best store PCR product? - (reply: 2)
  547. RT-PCR primer design - (reply: 1)
  548. After PCR, One Band on 1.2% Agarose but 2 bands on 8M Urea, 8% PAGE - (reply: 1)
  549. Odd gel run following PCR - (reply: 28)
  550. Qiagen PCR Array Reagents? - (reply: 1)
  551. No or very weak band using 16s primers - (reply: 1)
  552. No PCR amplification with b-actin primers - (reply: 1)
  553. Is PCR efficiency higher if mutation is in the middle of primer? - (reply: 7)
  554. Site-directed, PCR works but no colonies - (reply: 2)
  555. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  556. Primer Design Help: GFP primers for Arabidopsis - (reply: 1)
  557. No PCR amplified with long primers - (reply: 10)
  558. PCR optimization: PCR vs qPCR - (reply: 4)
  559. should I do blunt end my pcr product before ligation - (reply: 2)
  560. PCR smear for genomic samples - (reply: 4)
  561. Smaller band appear under Main product with every primers sets from PCR - (reply: 3)
  562. another primer dilution question - (reply: 2)
  563. i need help with virus pcr - (reply: 2)
  564. Primer Efficiency - (reply: 2)
  565. Experimental set up for qRT PCR - (reply: 13)
  566. A faster way to pick single colony clones for PCR screening? - (reply: 3)
  567. Colony PCR works but DNA yeild very low after miniprep - (reply: 2)
  568. How should I optimize my PCR - (reply: 2)
  569. Second round of PCR after gel extraction fails miserably - (reply: 3)
  570. an effective way to do a yeast colony pcr - (reply: 2)
  571. Conversion of ng/ul of DNA to ng for a PCR reaction - (reply: 2)
  572. urgent help plz with RT-PCR - (reply: 3)
  573. oligo(dt) 15 vs random primers - (reply: 3)
  574. Help to optain a long PCR produc - (reply: 3)
  575. Multiple bands in eluted PCR product - (reply: 1)
  576. having nice sharp bands in PCR but no signal in Real-Time PCR - (reply: 5)
  577. Problems with two step RT-PCR - (reply: 2)
  578. Getting genomic DNA for PCR - (reply: 4)
  579. primer dilutions - (reply: 4)
  580. Genotyping PCR primers - (reply: 1)
  581. Conventional PCR - (reply: 1)
  582. PCR of entire plasmid followed by self-ligation for mutagenesis- what issues mig - (reply: 2)
  583. PCR product loss on cleanup - (reply: 2)
  584. MSP Primers not working - (reply: 6)
  585. Primers too dilute - (reply: 1)
  586. Amplification curves jagged - (reply: 1)
  587. Cloning HELP- Possible Primer issues - (reply: 6)
  588. No PCR product in site-directed mutagenesis - (reply: 4)
  589. Giardia/Crypto real time PCR late amplification - (reply: 1)
  590. How to dilute the template DNA for PCR - (reply: 7)
  591. After I run my pcr on agarose the DNA is still in the well - (reply: 3)
  592. Need help for PCR - (reply: 5)
  593. PCR primer pairs - university exam - (reply: 7)
  594. unespecific band PCR from mouse genomic DNA - (reply: 9)
  595. Help with primer design through PRIMER3 - (reply: 4)
  596. Homozygous Heterozygous by PCR/Southern blot - (reply: 3)
  597. Normalizing to negative control primers for ChIP qPCR - (reply: 3)
  598. Plasmid vs cDNA amplification by PCR - (reply: 2)
  599. Tm of primers - prediction and verification - (reply: 1)
  600. PCR cloning...help! - (reply: 5)
  601. RT-PCR contamination in negative control. - (reply: 1)
  602. PCR malaria diagnosis nested PCR smear and non specific bands - (reply: 3)
  603. how to design primers for 16sr RNA - (reply: 24)
  604. how to do RT-PCR for16srRNA of a certain bacteria? - (reply: 4)
  605. Help with primer design - (reply: 3)
  606. Primer Tm calculation. - (reply: 1)
  607. low concentration of purified PCR product - (reply: 2)
  608. PCR smearing and...problems! - (reply: 7)
  609. Running Primers on Agarose Gel - (reply: 6)
  610. PCR product now not getting with same primers and other PCR conditions - (reply: 4)
  611. universal primers for PCR ribotyping - (reply: 6)
  612. will the template of PCR be added A ? - (reply: 2)
  613. Colony PCR not working - (reply: 4)
  614. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  615. Primer design: Tm above or below Ta? - (reply: 5)
  616. Oligo primers for shRNA construction - (reply: 1)
  617. Design primers for expression cloning - (reply: 11)
  618. Designing a synthetic gene to serve as the positive control for two separate PCR - (reply: 5)
  619. primer dilution query - (reply: 2)
  620. Real Time PCR polymerase enzyme - (reply: 3)
  621. Estimating size of PCR products in a agarose gel - (reply: 4)
  622. Sequencing of PCR product - (reply: 4)
  623. PCR contamination problem - (reply: 2)
  624. Using BLAST to check primers - (reply: 1)
  625. How to design RT-PCR primers in-frame for expression vector? - (reply: 1)
  626. What is the function of a third primer in a PCR mix?? - (reply: 2)
  627. How can we design a primer ? step by step method - (reply: 4)
  628. cDNA synthesis or RT-PCR - (reply: 3)
  629. PCR on cDNA and SGamplification??? - (reply: 1)
  630. EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay - (reply: 2)
  631. real time PCR protocol - controls? - (reply: 2)
  632. Long primers (60bp) and 5kb PCR product problems - (reply: 5)
  633. Abnormal plot of real time one-step RT-PCR, how to solve! - (reply: 1)
  634. SOS: new real time PCR machine needed.... which one? - (reply: 5)
  635. which step in the pcr cleanup process could harm the cohesive ends - (reply: 1)
  636. My product of PCR haven't the lenght right after purification....... What - (reply: 2)
  637. Is it possible to amplify cDNA with hexamer primers or oligo dT primers to have - (reply: 1)
  638. PCR and UV spectrophotometry - (reply: 3)
  639. RT-PCR in a single tube - (reply: 3)
  640. why Phusion polymerase is not recommended for overlap PCR? - (reply: 1)
  641. annealing temperature for 3 primer pairs? - (reply: 4)
  642. PCR Reaction - (reply: 3)
  643. Impact of primer sequence on TA cloning - (reply: 3)
  644. problems with Bisulfite PCR - (reply: 1)
  645. low pcr product - (reply: 3)
  646. overlapping PCR protocol - (reply: 12)
  647. PCR to amplify my insert not working - (reply: 6)
  648. Problem with PCR amplification- high 3' stability - (reply: 3)
  649. Home made real-time PCR problem - (reply: 4)
  650. High RNase concentration interfering with PCR? - (reply: 8)
  651. Pcr primers - (reply: 2)
  652. PCR failure from yeast genomic DNA - (reply: 1)
  653. failure of PCR from yeast genomic DNA - (reply: 1)
  654. RT-Q PCR primer design question - (reply: 2)
  655. high MW dimers - (reply: 2)
  656. Strange RT-PCR amplification plots - (reply: 9)
  657. How to clean extracted faecal DNA? (removing PCR inhibitors) - (reply: 1)
  658. What products will I get from this pcr? - (reply: 1)
  659. PCR buffer without Tris - (reply: 1)
  660. PCR Water - (reply: 4)
  661. multiplex rtqPCR and custom primer/probe design - (reply: 2)
  662. Which Brand of real-time PCR machine - (reply: 6)
  663. Problem with PCR - large extra bands - (reply: 3)
  664. big differnece in my primers Tm - (reply: 2)
  665. Designing primers for the ORFs - (reply: 9)
  666. Two-Step PCR - (reply: 2)
  667. Gradient PCR - (reply: 2)
  668. PCR with phophorylated primers - (reply: 1)
  669. Problem with New qPCR primers - help! - (reply: 6)
  670. Designing primers for miRNA located on minus strand. - (reply: 2)
  671. Plasmid sequencing without primers - (reply: 1)
  672. Smallest insert in PCR - (reply: 6)
  673. troubleshooting RT-PCR: possible nuclease issues? - (reply: 2)
  674. loop-mediated amplification - (reply: 2)
  675. Blunt end PCR product ligation - (reply: 1)
  676. Help with high Ct values for Real-Time PCR - (reply: 1)
  677. Genotyping by allele specific PCR - (reply: 6)
  678. RT-PCR primers and the poly(A) tail - (reply: 2)
  679. NO Insert found in the Colony PCR - (reply: 15)
  680. No band in PCR - (reply: 5)
  681. overlapping primer - (reply: 1)
  682. PCR primers help - (reply: 5)
  683. PCR smear after one-month storage - (reply: 2)
  684. Primer dimers - (reply: 2)
  685. RT-PCR inhibited after Turbo DNase treatment - (reply: 3)
  686. Real-Time PCR using ABI Fast Sybr Green chemistry - (reply: 2)
  687. Simple question of dNTP's for PCR - (reply: 5)
  688. Need help for PCR for AT rich gene - (reply: 3)
  689. How long I can keep my template added PCR reaction mix before running PCR? - (reply: 4)
  690. Primer validation and PCR efficiency - (reply: 4)
  691. How to calculate the price of PCR test per sample? - (reply: 6)
  692. Contamination in negative control of PCR (No template control) - (reply: 6)
  693. cloning with nested pcr - (reply: 3)
  694. What is the maxium amount of glycerol can be added in a PCR reaction? - (reply: 3)
  695. PCR is amplifying non-specific fragment - (reply: 6)
  696. ChIP amplification problems - (reply: 1)
  697. real time RT-PCR problems - (reply: 2)
  698. should I design primers spanning both intron and exon - (reply: 2)
  699. RT-PCR - (reply: 1)
  700. RNA extraction from FFPE cancer tissues for real-time RT-PCR - (reply: 2)
  701. Template DNA (plasmid) concentration for PCR - (reply: 4)
  702. PCR with a very long and a short primer - (reply: 5)
  703. Ligation of 16kb PCR product - (reply: 5)
  704. Multiplex PCR does not work - (reply: 3)
  705. Help: Linear Amplification Using Hydrolysis Probes - (reply: 3)
  706. Problem with genomic PCR (2.4 kb) - (reply: 1)
  707. Comparison between two primers pairs? Is it possible? - (reply: 3)
  708. Rt-PCR primer design - (reply: 7)
  709. Length of non-binding sequence in primers with RE site - (reply: 6)
  710. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  711. Getting a smaller PCR product than required - (reply: 6)
  712. Difficult sequences in PCR - (reply: 2)
  713. why is real time pcr curve like this? - (reply: 3)
  714. PCR inconsistency - (reply: 2)
  715. Orientation of primers - (reply: 2)
  716. PCR, neg control & no. of cycles - (reply: 2)
  717. Strange PCR result from genomic DNA - (reply: 3)
  718. Bright bands of PCR products stick in gel wells - (reply: 1)
  719. methylation analysis with MS-HRM (primer design) - (reply: 1)
  720. whole plasmid pcr? - (reply: 6)
  721. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  722. salts in PCR - (reply: 4)
  723. Strange amplification plot - (reply: 4)
  724. Conventional PCR of different dilutions - (reply: 1)
  725. primer resuspension - (reply: 2)
  726. Principles of overlap PCR - (reply: 7)
  727. ligation of pcr product into expression vector - (reply: 4)
  728. Unable to PCR!!!! - (reply: 3)
  729. Help designing primers with restriction sites - (reply: 3)
  730. Why primers shouldn't have Tm difference greater than 5°C? - (reply: 5)
  731. Low A260/A280 ratio after gel purification using Promega PCR Clean-up System - (reply: 2)
  732. Abnormal amplification curve - (reply: 1)
  733. Problem with Semiquantitative PCR - (reply: 4)
  734. Advice on optimising bisulfite PCR sought - (reply: 2)
  735. Help: I am loosing the DNA during PCR purification - (reply: 7)
  736. PCR primer with no extra DNA end - (reply: 5)
  737. PCR colonies of Pichia pastoris - (reply: 1)
  738. ROX reference dye for quantitative RT-PCR - (reply: 1)
  739. PCR problem - (reply: 9)
  740. why PCR product smeared? - (reply: 2)
  741. Tm calculation for primers with RE sites and overhangs - (reply: 4)
  742. silincing box, no amplification - (reply: 3)
  743. Comparative semiquantitative RT-PCR - (reply: 2)
  744. Bands in my PCR Controls - (reply: 3)
  745. cloning 2 PCR products into pGEM-T easy vector - (reply: 1)
  746. Strange band in colony PCR - (reply: 2)
  747. In a pcr why doesnt the DNA reneal during annealing process ? - (reply: 2)
  748. Multiplex PCR - (reply: 12)
  749. Real time pcr primers JunB, Foxh1, Klf4, PRMT1, PRMT4 and PRMT5 needed! - (reply: 2)
  750. PYO pcr stopped working. - (reply: 1)
  751. Bands seen with qPCR missing in regular PCR - (reply: 5)
  752. checking msp primers - (reply: 2)
  753. PCR product one day, none the next day - (reply: 4)
  754. PCR on colony - (reply: 10)
  755. PCR reaction ISSUES???? need help - (reply: 4)
  756. PCR using CDNA as template - (reply: 2)
  757. RT-PCR doesn't work with all RNA used - (reply: 1)
  758. Need help amplifying repeating sequence. - (reply: 5)
  759. Real Time PCR 101...help! - (reply: 1)
  760. RT-PCR primer design for full length cDNA cloning - (reply: 6)
  761. problems with PCR confirmation of insert . HELP ! - (reply: 1)
  762. Another primer dimers problem - (reply: 23)
  763. Colony PCR Problem!!!! - (reply: 5)
  764. using PCR product as standard template - (reply: 3)
  765. PCR kit - (reply: 7)
  766. Make additional cycles with an already finished pcr product - (reply: 3)
  767. Amplification in water control but not in samples - (reply: 3)
  768. PCR products form with DNA, but not with c-DNA - (reply: 2)
  769. question about pcr amplification efficiency? - (reply: 1)
  770. DNA polymerase: recombinant or native? - (reply: 3)
  771. pcr clean-up fail? - (reply: 1)
  772. MSP U primers very difficult to amplify... - (reply: 4)
  773. No gene amplification, 18s amplification is fine - (reply: 1)
  774. ChIP-qPCR gives odd amplification plot - (reply: 5)
  775. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  776. sequential cloning of multiple PCR products - how to do? (reply: 3)
  777. SYBR QPCR problem, please help! strange melting curve... - Primer dimers? Or other problems? (reply: 4)
  778. Primer design - (reply: 3)
  779. Tomato Actin PCR+Images included - (reply: 2)
  780. single primer PCR - (reply: 5)
  781. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  782. cDNA synthesis + amplification - (reply: 2)
  783. Design strategy for real-time RT-PCR, how to decide on what gene of interest and - Newbie that's completly mRNA confused, please help! (reply: 2)
  784. LacZ PCR Problems - (reply: 1)
  785. cells stably expressing T7 RNA polymerase - (reply: 1)
  786. Primer concentration - (reply: 4)
  787. nested PCR with low target DNA? - (reply: 2)
  788. RT-PCR calibrator - (reply: 2)
  789. RNA - extraction and pcr (reply: 1)
  790. problem in pcr - (reply: 2)
  791. Can someone recommend me a virtual PCR software? - (reply: 6)
  792. Trouble with PCR and electroporation - Trying to make mutants via PCR (reply: 4)
  793. Primer 3' mismatch - Strange experience (reply: 9)
  794. real-time PCR after restriction enzyme digestion - (reply: 6)
  795. wrong plot amplification in one-step RT- PCR - one step real time RT PCR (reply: 1)
  796. Adding loading/tracking dye to PCR mix? - (reply: 10)
  797. PCR: Band in one lane, streaking in the other..HELP! - (reply: 3)
  798. DNA polymerase with single G 3'-end overhang activity - (reply: 3)
  799. Primer BLAST and NetPrimer vs Primer3Plus - (reply: 3)
  800. relative expression of genes using semiquantitative PCR - Semi-quantitative PCR (reply: 1)
  801. how to get rid of non specific bands in PCR - (reply: 1)
  802. PRIMER DILUTION - STOCK SOLUTION TO A WORKING STANDARD (reply: 2)
  803. PCR tubes mixer & spinner ( one device ) - recommendations ? (reply: 2)
  804. which exon to choose when designing primers for RT-PCR? - splicing isoforms are different between NCBI and Genecard (reply: 3)
  805. PCR cloning problem - could not get colony (reply: 4)
  806. pcr contamination - (reply: 4)
  807. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  808. Large Non-specfic bands in PCR - (reply: 3)
  809. PCR clonning - (reply: 3)
  810. Difference between PCR/cloning DNA in plasmids - (reply: 2)
  811. 2 bands in blue colony PCR - (reply: 5)
  812. Primer dilution --> problem.. - (reply: 16)
  813. Help! Smear in Real-Time PCR Product - (reply: 1)
  814. alternative to Tris buffer in strand displacement (BST polymerase) - looking into alternatives to the NEB ThermoPol buffer (reply: 2)
  815. Sybr green RT-qPCR primer - (reply: 1)
  816. Sybr green RT-qPCR primer - (reply: 1)
  817. primer design - microalgae (reply: 2)
  818. Designing primers - What is more important Ta or primer's Tm - (reply: 3)
  819. Please help! no PCR bands+Description of my PCR protocol provided :( - (reply: 8)
  820. real time rt PCR - bloodyurine - inhibition - (reply: 1)
  821. PCR yield in ng/ul - (reply: 1)
  822. No Amplification in PCR - (reply: 4)
  823. Real time PCR and percentage loss - (reply: 1)
  824. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 3)
  825. Problem with smear in PCR with certain templates - (reply: 4)
  826. Real time PCR in the presence of heme - (reply: 3)
  827. issues in PCR amplification - (reply: 2)
  828. Problems with PCR in general - (reply: 1)
  829. Qiagen RT-PCR Kit (Rotor-Gene Q) - Which one should I use? (reply: 2)
  830. how to overcome primer contamination - need protocol (reply: 11)
  831. RT-PCR primer design - Primer design and evaluation (reply: 7)
  832. CHIP analysis by PCR - (reply: 4)
  833. STANDARDISATION OF MULTIPLEX PCR - IN order to standardise a multiplex PCR for 3 set of primers (reply: 3)
  834. Primer,probes dilution - (reply: 3)
  835. Splice varient quantification in RT real time PCR? - (reply: 1)
  836. negative control for msp pcr - (reply: 2)
  837. Calculating geometric mean for real-time PCR - (reply: 5)
  838. template transfer? - how does the rna polymerase switches template? (reply: 1)
  839. PCR Gel nonspecific band - (reply: 13)
  840. semiquantitative pcr - (reply: 1)
  841. Sudden problems with cDNA PCR using Phusion Polymerase - (reply: 9)
  842. PCR: Cloning Primers - (reply: 8)
  843. PCR product - (reply: 5)
  844. pcr amplify an insert in plasmid? - (reply: 2)
  845. Sybr Green Real Time PCR - Amplification plot - Any problem? (reply: 1)
  846. Exon Spanning PCR Primers - (reply: 1)
  847. Colony PCR - (reply: 1)
  848. PCR to confirm double crossover - (reply: 1)
  849. Primer sequences - (reply: 1)
  850. Why does freezing/thawing DNA samples first improve PCR results? - (reply: 8)
  851. smears in PCR products (bisfulite treated DNA) - (reply: 4)
  852. PCR Primers - (reply: 2)
  853. Cleaning up RT-PCR product before sequencing - (reply: 1)
  854. Template Amount for PCR Amplification - (reply: 6)
  855. How to blast methylation specific and unspecific primers - (reply: 1)
  856. RT-PCR cDNA synthesis - (reply: 6)
  857. problems amplifying from cDNA - (reply: 4)
  858. Real-time PCR about Incorrect setup of the data collection stage - (reply: 1)
  859. No DNA after PCR purification with QiaQuick? - (reply: 4)
  860. restriction digestion against colony PCR - (reply: 1)
  861. How to check primers are of correct sequence or not? - (reply: 2)
  862. Primers... - (reply: 1)
  863. When are primer dimers a problem? - (reply: 1)
  864. Inverted repeat: three-way ligation or fusion PCR? - (reply: 3)
  865. PCR: always got band in the negative control - (reply: 8)
  866. primer with higher melting temperature - (reply: 2)
  867. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  868. Problems designing primers for BSP - (reply: 2)
  869. qPCR primer design - possible off-target (reply: 1)
  870. Primer dimer formation and real-time PCR - (reply: 7)
  871. Identify PCR products - (reply: 3)
  872. PCR genomic DNA using cell lysate - (reply: 1)
  873. Maximum size of overhangs in PCR - (reply: 6)
  874. Colony PCR doesn't work anymore - (reply: 5)
  875. RT-PCR t-test, and ANOVA - (reply: 1)
  876. Primer Design Urgent! - (reply: 1)
  877. PCR basics - I'm new to all things lab and need some help! (reply: 3)
  878. Able to see correct product with 2 rounds of PCR, but my PI doesn't want me - How do I convince her that "2 rounds of PCR is OKAY?" (reply: 1)
  879. PCR of microRNA first strand cDNA - (reply: 1)
  880. how to know my primer's sense ??? - help ! (reply: 2)
  881. smear in pcr +DNA polymerase mixture for long PCR - (reply: 10)
  882. High Ct value in Real Time RT PCR for NTC - Real Time RT PCR troubleshooting (reply: 5)
  883. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  884. intron spanning primers for non model organisms - (reply: 2)
  885. Primer design - (reply: 2)
  886. Troubleshoot PCR, Product band missing, internal control is present - (reply: 1)
  887. qPCR amplification interference fixed with freezing - (reply: 6)
  888. Suggest me a mastermix for conventinal PCR... - (reply: 11)
  889. software for quantifing PCR product band - (reply: 1)
  890. Multiple bands from purified PCR product - (reply: 3)
  891. Sybr Green Real Time PCR - Melt curve - Any problem on the melt curve? (reply: 3)
  892. Doing PCR on Nebulized DNA - (reply: 2)
  893. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  894. RT-PCR - (reply: 9)
  895. help with negative strand specific primer design needed - Primers for Sindbis virus (-) strand (reply: 4)
  896. inverse pcr - (reply: 2)
  897. Will I need interplate calibration for my RT-PCR experiment? - (reply: 3)
  898. Primer seq - (reply: 2)
  899. Urgent: should you dry PCR product - (reply: 13)
  900. PCR product size - (reply: 6)
  901. amplification plot raises to early - What is the explanation for this problem? (reply: 1)
  902. oligo dt and random primer - (reply: 3)
  903. mRNA Search for RT-PCR (U to T) - (reply: 2)
  904. First-strand cDNA synthesis from paraffin embedded RNA - Using of gene-specific primers (reply: 1)
  905. PCR Mastermix - (reply: 4)
  906. PCR triplicates versus one reaction - differences? (reply: 17)
  907. Comparing Gene Expression of Different Genes Using Semi-Quantitative PCR - (reply: 2)
  908. Do low binding 0.2ml PCR tubes exist? - (reply: 3)
  909. Primers and self and hetero dimers - (reply: 1)
  910. Really weird amplification curves - (reply: 1)
  911. Primers for qPCR - (reply: 3)
  912. Placing dNTP in 64 C waterbath - Would this ruin the solution? (reply: 3)
  913. RT-PCR primer - (reply: 3)
  914. PCR problem - basic PCR for plasmid amplification (reply: 2)
  915. Weird bands in standard PCR of gDNA and cDNA - (reply: 2)
  916. mutation using overlapping PCR, what's the success rate? - (reply: 3)
  917. Unpredictablity of PCR product - (reply: 5)
  918. total cDNA amplification by PCR - (reply: 2)
  919. Detection limit of a conventional PCR - (reply: 2)
  920. PCR for MULTIPLE mutagenesis - (reply: 4)
  921. Using Sybr green in realtime pcr - (reply: 1)
  922. IF ITS1 and ITS4 using as primer and working on the fungi . Shall we get single - (reply: 12)
  923. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  924. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  925. Dissolved Primer pellet in pure ethanol instead of water - (reply: 1)
  926. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  927. Dissolved Primer pellet in pure ethanol instead of water - (reply: 2)
  928. Dissolved Primer pellet in pure ethanol instead of water - (reply: 4)
  929. Digested PCR product migrate slower than uncut - (reply: 8)
  930. Left polymerase above fridge for more than 1 week - still working? (reply: 3)
  931. PCR question - (reply: 2)
  932. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  933. 2 rounds PCR got problem - (reply: 2)
  934. PCR help minus strand - Primer design (reply: 1)
  935. chloramphenicol storage and amplification - (reply: 3)
  936. Smear in long distance PCR - (reply: 39)
  937. TP-PCR or three primer PCR - TP-PCR or three primer PCR (reply: 9)
  938. Help - I m trying to get amplification for a new gene with new set of primers (reply: 2)
  939. TOUCH DOWN PCR - (reply: 2)
  940. Source of RNA for pcr efficiency, when to use ref gene? - (reply: 3)
  941. PCR stopped working - After changing buffer (reply: 5)
  942. ChIP PCR question - (reply: 33)
  943. PCR protocol questions! - (reply: 2)
  944. TOUCH DOWN PCR NEEDS EXPLANATION - (reply: 3)
  945. DNA amount calculation for PCR - (reply: 14)
  946. ChIP primer design - (reply: 1)
  947. Is T4 DNA Polymerase so evil? Blunt End Cloning - (reply: 3)
  948. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  949. Using digoxigenin in PCR? - Want to create a FISH probe (reply: 2)
  950. How to analyze ChIP PCR data - (reply: 10)
  951. Questions regarding RT-PCR optimization - (reply: 2)
  952. number of copies after pcr? - (reply: 1)
  953. PCR double-bands - (reply: 1)
  954. Problems with semi-quatitative PCR - (reply: 1)
  955. Question about Primers - (reply: 3)
  956. Odd question. Can I use DNase to decontaminate Primers? - Primer decontamination (reply: 2)
  957. Large fragment amplification failed - (reply: 3)
  958. purification of PCR product for cloning in a vector - (reply: 3)
  959. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  960. PCR reaction calculation - (reply: 2)
  961. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  962. Primer design with a tag - (reply: 4)
  963. no increase in fluorescence in my real time PCR - (reply: 3)
  964. PCR Problem: Inconsistency with the results since one week - Inconsistency (reply: 1)
  965. Help with real-time PCR quantification of miRNA - (reply: 8)
  966. PCR, using gel extracted band as a template - (reply: 4)
  967. PCR Amplification: Tips on simple things to keep an eye out for - (reply: 1)
  968. Primer3 and Blast - how can I search for primers against a local database. (reply: 3)
  969. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  970. Combining forward and reverse primer gives different size on gel - (reply: 2)
  971. Q-PCR and RT-PCR - (reply: 3)
  972. calculating total amount - in a pcr reaction (reply: 4)
  973. Problems with amplifying microRNA - (reply: 3)
  974. PCR of complement genomic DNA - (reply: 3)
  975. primers for fungal identification - (reply: 9)
  976. confusing PCR - (reply: 1)
  977. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  978. PCR for long and repetitive region from genomic DNA - (reply: 4)
  979. difference between PCR primers and sequencing primers - (reply: 1)
  980. How do I PCR a DNA fragment with >200 CGG repeats? - (reply: 2)
  981. PCR of repeated region - (reply: 1)
  982. Sense and antisense DNA, and primer design - (reply: 6)
  983. PCR bands in NTC but NOT in negative samples - (reply: 2)
  984. housekeeping genes in PCR - (reply: 1)
  985. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  986. Question about RealTime PCR Primer Design - (reply: 1)
  987. Designing a Primer with a RS that has a W - (reply: 1)
  988. Primers stopped working!? - PCR Primers (reply: 6)
  989. NFK beta primers - (reply: 4)
  990. final PCR product - need clarifications (reply: 1)
  991. Mutagenesis PCR problem - Help with PCR mutagenesis (reply: 5)
  992. Is DNase necessary for primers designed on the exon-exon boundary?? - (reply: 2)
  993. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  994. RT-PCR result !! - (reply: 4)
  995. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  996. Troubleshooting help: Why do my amplification curves look like this? - (reply: 8)
  997. Agarose gel electrophoreses for PCR products ? - (reply: 4)
  998. why genomic can't be use for pcr? - (reply: 2)
  999. Primer design - Primer design (reply: 2)
  1000. Re-use unamplified PCR product - (reply: 2)
  1001. Reason for odd PCR conditions - (reply: 3)
  1002. Amplification of region from sperm RNA - sperm RNA stability (reply: 4)
  1003. Amplification of 3.4 kb product from RNA for cloning - (reply: 5)
  1004. pcr - problem in pcr (reply: 2)
  1005. Primers for PCR - Forward and Reverse Primer Sequences (reply: 4)
  1006. How to present and analyze these real-time PCR data? - (reply: 2)
  1007. Deoxyribosenucleoside vs. Deoxyribosenucleotide - What does dNTP stand for? (reply: 2)
  1008. RT-PCR: DNA contamination, one vs two step - RNA work (reply: 11)
  1009. PCR Master mix - (reply: 2)
  1010. cDNA storage and real time RT PCR - (reply: 3)
  1011. Real Time PCR Primers and Probes - (reply: 2)
  1012. Anyone make their own PCR cloning vector? - (reply: 5)
  1013. Stability of PCR T-overhangs - (reply: 1)
  1014. Loss of bands at higher Tm in presence of DMSO... - Attempt to optimise DMSO methylation-spec PCR (reply: 4)
  1015. PCR product wrong size! Need help! - (reply: 9)
  1016. pooling different pcr cycles? - (reply: 1)
  1017. Amplification of concatenated linear ligated fragments - (reply: 4)
  1018. Clinical Real-time PCR assay - (reply: 1)
  1019. Need help - How we can design primer for miRNA (reply: 1)
  1020. primer and promoter sequences - (reply: 1)
  1021. Standard curve for real-time PCR - (reply: 2)
  1022. designing of primers - (reply: 2)
  1023. Primer Tm differences/ PCR - (reply: 1)
  1024. Primer design to screen silenced lines - Primer design tips to screen silenced lines (reply: 3)
  1025. single gene amplification - cDNA synthesis and amplification with gene specific primers (reply: 2)
  1026. d2EGFP Primers for Genotyping - ...where to get the sequence? (reply: 5)
  1027. BAC recombineering problem - BAC clones positive for double selection but negative for PCR, why? (reply: 1)
  1028. Designing primer to remove his-tag - (reply: 1)
  1029. PCR Primer Dilution from F+R - (reply: 1)
  1030. primer reconstitution - primer reconstitution (reply: 4)
  1031. Not-Quite-Nested PCR - (reply: 2)
  1032. PCR product too short - (reply: 6)
  1033. Anyone know the margin of error for PCR amplicon size visualized on an agarose g - Margin of Error (reply: 2)
  1034. Primer design for insert amplification - Is this correct? (reply: 2)
  1035. got smear on the PCR gel !!! - (reply: 4)
  1036. need to know minimum amount of template DNA needed for pcr amplification - pcr (reply: 2)
  1037. "Unzipped" PCR band - (reply: 3)
  1038. Review on new amplification techniques - (reply: 2)
  1039. What's the highest DNA concentration for PCR? - before being inhibited? (reply: 7)
  1040. DOT BLOT for pcr product - No result obtain (reply: 1)
  1041. melting temperature in real time pcr - melting temperature in real time pcr (reply: 5)
  1042. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  1043. HRM primers - (reply: 1)
  1044. PCR amplification with Pfu / quality of DNA - (reply: 4)
  1045. primer design - (reply: 2)
  1046. Is it possible to compare data between real-time PCR plates - (reply: 1)
  1047. Conventional PCR problems - wisamni2004 (reply: 2)
  1048. New to sequencing, primer design - (reply: 2)
  1049. Is it possible to know the live bacteria using PCR for a certain genus? - (reply: 5)
  1050. Is it possible to know only the live bacteria using PCR for a certain genus? - (reply: 1)
  1051. incorrect/smaller band size for real time PCR - (reply: 1)
  1052. Quickchange mutagenesis primer - (reply: 3)
  1053. pcr / primer theory in bisulfite sequencing - (reply: 4)
  1054. homologous amplicon for real time pcr - (reply: 1)
  1055. Primer Design..need very basic advice. - Please help, I cant figure this out for the life of me. (reply: 2)
  1056. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  1057. assistance with PCR amplification please - (reply: 3)
  1058. I need an urgent help with q-pcr amplification plots - (reply: 11)
  1059. Problems with the Specificity of the Primers - (reply: 2)
  1060. confusion in real time PCR primers - Confusion in Real time pcr primers (reply: 3)
  1061. Colony PCR with FALSE Positive Results ?? - (reply: 4)
  1062. Degenerate primers PCR problem, Please help! - (reply: 2)
  1063. Hotstart PCR and unspecific Amplification - (reply: 3)
  1064. Primer design help needed - (reply: 1)
  1065. Primers no longer work - (reply: 1)
  1066. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  1067. Primer Design with Primer-Blast - My experience has been worse than just using Primer3 and BLAT separate (reply: 4)
  1068. RT PCR for known snps detection? - SNPs detection (reply: 1)
  1069. colony PCR - (reply: 2)
  1070. Calculating Primer concentrations for PCR - Is there an easy way to do this...help (reply: 3)
  1071. primer Tm is too high, how tu get pcr product - help! (reply: 21)
  1072. PCR reaction without template gives a product - (reply: 6)
  1073. PCR wrong product size - (reply: 6)
  1074. Wrong pcr product size - (reply: 4)
  1075. real time PCR machine - (reply: 5)
  1076. PCR very faint product - (reply: 1)
  1077. Question about principle of PCR - (reply: 3)
  1078. PCR amplification before bisulfite conversion - (reply: 6)
  1079. 4 question for real time PCR - (reply: 5)
  1080. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  1081. PCR didn't work - suspect the enzyme (reply: 2)
  1082. Find universal primers in vector - any online tool? (reply: 6)
  1083. Picking primers to confirm Illumina meth27 results - (reply: 2)
  1084. Colony PCR Specificity - (reply: 2)
  1085. Colony Pcr - primers - (reply: 5)
  1086. iCODEHOP help: how are the reverse primers supposed to be read? - Degenerate primer design (reply: 1)
  1087. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  1088. Primer Dilution Problem - D'oh! (reply: 2)
  1089. Using PCR to create overhangs - (reply: 3)
  1090. Creating overhangs with PCR - (reply: 4)
  1091. Problem with PCR on bacmids - (reply: 1)
  1092. Is there any software that can be sued to design degenerate primers - (reply: 1)
  1093. Running real time pcr product on gel?.. - (reply: 4)
  1094. Primer Decontamination - (reply: 2)
  1095. PCR mutagenesis of plasmid - (reply: 5)
  1096. tools for checking non-specific binding of primers -
    (reply: 1)
  1097. Primer designed for ARMS PCR - (reply: 2)
  1098. Problem with Telomeric Length measurement using RT-PCR - (reply: 1)
  1099. methylation specific PCR - Primers - (reply: 1)
  1100. bacterial identification using real time PCR - (reply: 1)
  1101. Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (reply: 2)
  1102. real time PCR inhibition control - a test for the presence of PCR inhibitors (reply: 3)
  1103. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  1104. colony PCR for subcloning gene - (reply: 1)
  1105. Problems with Multiplex PCR - Problems with Multiplex PCR (reply: 4)
  1106. Using the same PCR plate in more runs - Just curious about it. (reply: 1)
  1107. PCR reamplification - (reply: 2)
  1108. Need advice on a smear from primer-primer binding PCR reaction. - (reply: 2)
  1109. How many colonies to screen (colony PCR)? - (reply: 4)
  1110. Fusion PCR, bright smear from well to end(with very weak or no band) - (reply: 1)
  1111. Primer Design - aligning sequences for probe/primer design (reply: 2)
  1112. question about pcr - (reply: 3)
  1113. RT-PCR Taqman no change in gene expression - GATA3 gene expression in splenocytes, a tough one (reply: 3)
  1114. How to construct a standard curve for real time PCR - (reply: 1)
  1115. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  1116. PCR with one primer - (reply: 1)
  1117. colony PCR inconsistent - (reply: 4)
  1118. PCR contamination - (reply: 19)
  1119. PCR failed No band.. desperate for opinions.. gel image available - (reply: 2)
  1120. If a 20bp primer differs from template DNA by 20bp, can PCR work? - (reply: 1)
  1121. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  1122. PCR not working - No amplification - (reply: 5)
  1123. Quantification of PCR products - (reply: 1)
  1124. fermentas clonejet pcr cloning kit - want opinion (reply: 1)
  1125. Stratagenes Dpn1 cleaves my PCR product - (reply: 1)
  1126. qPCR Amplification Efficiency too high - Efficiency of 115 - 145% (reply: 1)
  1127. Help... my PCR don't come any more - PCR amplification desapeard (reply: 3)
  1128. Nested PCR question - (reply: 1)
  1129. High Tm on primers - cannot get a product - (reply: 8)
  1130. Help: Very odd amplification graphs (RTPCR) - (reply: 3)
  1131. undesired products in multiplex PCR - (reply: 3)
  1132. NCBI primer blast problem - (reply: 1)
  1133. Help with Multiplex Nested PCR - (reply: 2)
  1134. Taq polymerase works but Phusion polymerase doesn't - How to achieve high-fidelity with robust amplification? (reply: 3)
  1135. Question of q-pcr primer design - Tm difference from IDTDNA (reply: 2)
  1136. primer sequence problem - (reply: 2)
  1137. Finding bisulfite PCR primer sequence - (reply: 3)
  1138. PCR -No band formation - (reply: 1)
  1139. PCR - No Band formation - (reply: 3)
  1140. No amplification after bisulfite treatment - (reply: 4)
  1141. PCR AMPLIFICATION - (reply: 1)
  1142. Ligation of Blunt PCR Product - (reply: 1)
  1143. real time pcr need advise - (reply: 2)
  1144. Opinion about LUX primers /D-LUX assay - What say you? (reply: 5)
  1145. PCR & Cloning - (reply: 6)
  1146. Long PCR Primers? Advice for generating multiple adjacent mutations - Designing long degenerate primers for shotgun alanine scanning (reply: 3)
  1147. use of housekeeping gene in RT PCR chIP - (reply: 3)
  1148. DNA pooling for PCR - Saving money PCR (reply: 7)
  1149. Real Time PCR Standard curves - How many are required??? (reply: 1)
  1150. PCR need some help - (reply: 4)
  1151. How to design primers to check my candidates in a ChiP assay - (reply: 4)
  1152. Left polymerase out overnight - Will it still work? (reply: 1)
  1153. Primer Reconstitution--does temperature matter? - (reply: 5)
  1154. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  1155. Primers that do not align at regions with SNPs. Where to find SNPs? - (reply: 2)
  1156. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  1157. Gradient PCR works, but single temperature does not! HELP!! - (reply: 5)
  1158. Primer efficiency - How to determine primer efficiency correctly? (reply: 4)
  1159. no PCR product from input... - (reply: 8)
  1160. MSP - unwanted amplification - (reply: 5)
  1161. Smears in Bisulfite seq. PCR - (reply: 3)
  1162. Primer design for qPCR - (reply: 2)
  1163. Primer design for qPCR - (reply: 1)
  1164. DNA bisulphited Amplification - the amplification dont produce any band!! (reply: 3)
  1165. Pipettors for Real Time PCR (urgent) - Any good one? Any good recommended brands? (reply: 5)
  1166. Multiple bands after bisulfite PCR - (reply: 7)
  1167. PCR genomic DNA of high GC content - (reply: 6)
  1168. GAPDH primer design and efficiency problems (new to rt-pcr) - (reply: 4)
  1169. primer design tips - (reply: 3)
  1170. PCR with Biotin Incorporation - (reply: 2)
  1171. pcr product - hello all (reply: 1)
  1172. design pcr primer - (reply: 1)
  1173. promoter cloning PCR problem - what was wrong for my PCR set-up (reply: 3)
  1174. amplification of GroEL from Wolbachia! - amplification of GroEL from Wolbachia! (reply: 2)
  1175. PCR with long and complex primers - (reply: 2)
  1176. Phospholylation of primers - (reply: 1)
  1177. apoptosis by PCR? - (reply: 12)
  1178. Re-using PCR plates? - (reply: 2)
  1179. Touchdown PCR issues - (reply: 3)
  1180. LINE1 OFR2 Primer Design - (reply: 1)
  1181. phosphorylation of primers using PNK - (reply: 1)
  1182. RT-PCR contamination issue - (reply: 3)
  1183. Primers for yeast (18S) - (reply: 1)
  1184. How to reduce the bisulfite PCR bias? - (reply: 4)
  1185. What's the longest overhanging primer seq. you have used? - (reply: 2)
  1186. Primer Concentration to lower Ct - (reply: 1)
  1187. Problems with Stratagene's site directed mutagenesis kit - Primers, PCR, Mutagenesis (reply: 3)
  1188. Primer Concentration Help - (reply: 4)
  1189. who got a manual of ABI7500 PCR instrument ? - how to operate this instument ? (reply: 4)
  1190. Primer Degradation - What exactly happens? (reply: 1)
  1191. Smearing on ategarose gel of real time pcr product??? - (reply: 1)
  1192. PCR/DNA Extraction Problem - (reply: 5)
  1193. Primers in 2 exons - (reply: 2)
  1194. NTC appear in real time pcr - (reply: 6)
  1195. Problems with designing a primer - (reply: 2)
  1196. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  1197. PCR of AT-rich DNA - (reply: 1)
  1198. T7 RNA polymerase transcription and translation. - The maximum number of nucleotides between T7 promoter and first ATG (reply: 2)
  1199. Overlapping PCR - really need help ! - (reply: 5)
  1200. Freezing PCR product??? - (reply: 1)
  1201. problem with pcr cloning from mouse cDNA - (reply: 2)
  1202. Probe or Primer info for bovine AQP-1 - (reply: 6)
  1203. PCR with Platinum Taq - product yield issues - (reply: 3)
  1204. real time PCR - (reply: 7)
  1205. Different sized product for same BS primer! - (reply: 2)
  1206. strategy of primer designing - difference between designing a primer for expression and cloning.. (reply: 1)
  1207. How do you estimate PCR product size ? - How do you estimate PCR product size ? (reply: 3)
  1208. PCR: cDNA works on 18S, but not Gap or designed primers - (reply: 2)
  1209. Real Time PCR Normalization - What if I have no choice for housekeeping genes to normalize RT PCR (reply: 3)
  1210. Differences between primers for real-time PCR and RT-PCR - (reply: 6)
  1211. primers with restriction site - (reply: 1)
  1212. PCR product appear two close band in my gel - (reply: 15)
  1213. The Case of a Missing Band: PCR Issue - (reply: 4)
  1214. PCR: Smear bands, no amplification, got it all.. - PCR Mutagenesis (reply: 5)
  1215. cDNA as PCR template - (reply: 1)
  1216. degenerate PCR - how to clone degenerate PCR products into a T/A cloning vector? (reply: 3)
  1217. cloning a PCR product. Taq pol or High Fidelity pol? - (reply: 6)
  1218. paranormal qpcr amplification activity - melting curves (reply: 3)
  1219. Can a primer bind to DNA in a 5'-3 parallel fashion? - Just wondering... (reply: 1)
  1220. PCR template concentration - (reply: 6)
  1221. Bands in PCR negative control - (reply: 3)
  1222. PCR efficiency calculated by Linreg - (reply: 3)
  1223. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  1224. primer design by generunner DG - (reply: 1)
  1225. degenerate pcr - (reply: 1)
  1226. BSP primers design, help please - (reply: 2)
  1227. primers with fluoresent dye PCR cycle question - (reply: 4)
  1228. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  1229. Smears on PCR products - (reply: 4)
  1230. Dumb Reverse Transcription PCR Question - (reply: 2)
  1231. Reverse transcription PCR - (reply: 1)
  1232. PCR/RT-PCR beads - (reply: 2)
  1233. High Amplification Efficiency in Std. Curve - (reply: 5)
  1234. Non specific products in Bisulphite pcr - (reply: 4)
  1235. Run PCR amplifications in agarose gels - (reply: 3)
  1236. Problems with validating primers and low expression genes - (reply: 3)
  1237. Real-Time PCR using genomic DNA (without DNA purification) - (reply: 3)
  1238. Bisulfite sequencing PCR not working - (reply: 5)
  1239. [Help]universal tag - universal tag in multiplex PCR (reply: 4)
  1240. Tips for visualizing very faint bands in agarose gels? - (The PCR ain't going to get better, so the gel must!) (reply: 15)
  1241. intron/exon spanning primers - (reply: 1)
  1242. can I use routine PCR to assess whether a gene is expressed or not? - (reply: 3)
  1243. expression cloning in TOPO TA and pET vectors - Very high and unusual non specific amplification in colony PCR (reply: 1)
  1244. PCR product for sequencing - sample storage?? (reply: 2)
  1245. Absolute quantification using Real Time PCR - (reply: 2)
  1246. PCR of AT rich gene - - I am having trouble amplifying an AT rich sequence (reply: 4)
  1247. question about blunt ligating Taq-amplified PCR product - (reply: 2)
  1248. RT-PCR Gel - Ladder looks terrible and product is fuzzy! (reply: 1)
  1249. MSP primer troubles - (reply: 3)
  1250. Measuring global methylation using real time PCR - (reply: 2)
  1251. Designing Primers for multiple Isoforms - (reply: 1)
  1252. RT-PCR standard curve dilutions - (reply: 2)
  1253. Rt-PCR problem !with 2.5 kb gene - (reply: 3)
  1254. real time pcr melt curve and primer efficiency problem - (reply: 3)
  1255. Aaaah I want to die!!!! PCR won't work - Why do the extractions that amplified 2 weeks ago fail now? (reply: 13)
  1256. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  1257. PCR product dimer issue - (reply: 24)
  1258. PCR additives Formamide - Formamide which one? (reply: 4)
  1259. PCR Master mix - (reply: 1)
  1260. Addition of Restriction sites into PCR primers - (reply: 4)
  1261. Polymerase Chain Reaction (PCR) - Anylyzing my PCR gel (reply: 6)
  1262. failure PCR amplification from low GC content gene - (reply: 5)
  1263. How is this idea sounds to you? - A new approach for rapid sample preparation for PCR (reply: 1)
  1264. conventional v real-time PCR applications - (reply: 1)
  1265. Primer Design in 3´non translated version vs. coding region - (reply: 2)
  1266. PCR reagents, can I use reagents from different manufacturers? - (reply: 6)
  1267. different PCR primers for real-time and classic PCR - (reply: 3)
  1268. primers deconamination??? - (reply: 6)
  1269. PCR band too thick - PCR troubleshooting question (reply: 5)
  1270. RT-PCR problem - (reply: 1)
  1271. Frameshift Mutation at the plasmid level - If your PCR product is sequence verified are you good to go? (reply: 5)
  1272. primer check!!! - (reply: 6)
  1273. PCR bands on gel electrophoresis - (reply: 5)
  1274. Qiagen Qiaquick vs MinElute PCR purification kit for ChIP DNA - (reply: 10)
  1275. long range PCR - (reply: 2)
  1276. Annealing Temperature of biotinylated primers - (reply: 2)
  1277. RT-PCR - New to RT-PCR info (reply: 2)
  1278. PCR punch - (reply: 4)
  1279. good amplification in classic PCR, no amplification in qPCR - (reply: 6)
  1280. PCR off plasmid for screening - (reply: 2)
  1281. PCR failed no product.. help - (reply: 10)
  1282. number of molecules in PCR - (reply: 3)
  1283. Freezing PCR products prior to ligation - To use a blunt-end vector or an overhang vector? (reply: 2)
  1284. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  1285. Primer clean-up - ExoSAP-It isn't working (reply: 6)
  1286. Primer dilution - PCR primer dilution (reply: 2)
  1287. Data acquisition for qPCR - Endpoint of annealing or endpoint of amplification? (reply: 3)
  1288. Can't re-PCR the PCR product - (reply: 5)
  1289. PCR reagent autoclave - (reply: 2)
  1290. Bad PCR, is it due to conditions, reagents, or lack of DNA template? - (reply: 1)
  1291. Long Primers for PCR - (reply: 2)
  1292. Data Analysis for Real-time PCR - (reply: 2)
  1293. PCR not working - overhangs too long? (reply: 3)
  1294. Apoptotic gene expression - Help required in RT-PCR for apoptotic genes (reply: 1)
  1295. argh more pcr headaches! - (reply: 6)
  1296. With 40 pcr cycles, how relevant are samples with Ct's of 35-40? - Help me please, I'm going nuts over this problem!! (reply: 2)
  1297. Chlamydia PCR cycling conditions - (reply: 1)
  1298. Primers going "loopy" - (reply: 2)
  1299. Conversion of primer unit - ug/ul to uM (reply: 4)
  1300. mutagenesis by PCR or just use a kit - (reply: 5)
  1301. Unsuccessful BS PCR - (reply: 3)
  1302. Primer Trouble Shooting - (reply: 5)
  1303. Proven Primers Stopped working & now leave smear! - (reply: 2)
  1304. Smearing on gel in PCR products - (reply: 5)
  1305. Ethanol in PCR clean-up - Can I trust lab ethanol stocks (reply: 3)
  1306. Relative RT-PCR, multiplate and calibration - (reply: 4)
  1307. Don't understand why we need RT-PCR? - Slight Confusion guys, please clear me up (reply: 6)
  1308. Relative RT-PCR - (reply: 2)
  1309. Relative real time RT PCR - (reply: 2)
  1310. DTT - WHY DTT IS USED IN RT- PCR (reply: 1)
  1311. 40 pcr cycles and Ct-values of 36-40 - trust or not? - (reply: 1)
  1312. a simple primer problem - (reply: 5)
  1313. Failure to introduce mutations using Overlap PCR - (reply: 3)
  1314. no or poor amplification - (reply: 1)
  1315. Primers from 3'UTR - (reply: 2)
  1316. Strange RT-PCR Graph - Is this inhibition of PCR? (reply: 1)
  1317. problem in plasmid isolation - unable to detect false positives in colony pcr (reply: 5)
  1318. Designing primers with ESTs - (reply: 2)
  1319. Direct sequencing of Bisulphite PCR product - (reply: 1)
  1320. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  1321. Huge difference in Tm of my For and Rev primers = no PCR product?!? - (reply: 11)
  1322. Real Time vs Traditional PCR results - (reply: 1)
  1323. The problems to identify mouse genotype with PCR - (reply: 1)
  1324. addition of BclI restriction site to PCR primer - (reply: 6)
  1325. non specific amplification - DNA amplification by PCR (reply: 1)
  1326. Molecular Cloning - Cloning of PAPD PCR products (reply: 1)
  1327. How does methprimer calculate primer Tm - (reply: 2)
  1328. Laminar Flow vs PCR cabinet - (reply: 4)
  1329. How to do PCR to detect mRNA without RT? - (reply: 2)
  1330. first strand cDNA synthesis not working - cannot PCR out cDNAs of interest from first strand cDNA (reply: 2)
  1331. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1332. PCR has stopped working - (reply: 7)
  1333. PCR negative control contamination - (reply: 8)
  1334. LacZ PCR genotyping -ve control contamination - (reply: 3)
  1335. Phosphorylated primers - (reply: 5)
  1336. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  1337. PCR efficiency in real timePCR - (reply: 5)
  1338. Amplification from plasmid DNA, but not from genomic DNA with the same target re - (reply: 4)
  1339. Drastic Decrease in PCR product yield - (reply: 2)
  1340. resources on PCR principles & technique - (reply: 1)
  1341. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  1342. Colony Screeing without using PCR - (reply: 8)
  1343. High MW PCR band seen...help needed - (reply: 7)
  1344. QPCR - Primer and Probe question - QPCR (reply: 2)
  1345. Urgent Help needed: RNA-Interference, rt-pcr and Western-Blot do not match - (reply: 2)
  1346. standard curve/ PCR efficiency problems... - (reply: 1)
  1347. Help! PCR that used to work doesn't work now! - (reply: 2)
  1348. PCR and templates - (reply: 3)
  1349. does anyone have experience with pwo DNA polymerase for long PCR fragments ? - (reply: 2)
  1350. Real Time PCR internal reference (housekeeping) gene in E. coli - is rrsB suitable (reply: 1)
  1351. PCR problems on high GC content gene - Trouble with Colony PCR of TOP10 transformants w/ TOPO-vector (reply: 4)
  1352. Easy primer question? - Primers (reply: 1)
  1353. PCR inconsistency - (reply: 4)
  1354. Plasmid problem - From PCR product (reply: 7)
  1355. help needed: PCR a gene from genomic DNA - (reply: 2)
  1356. questions about pcr products after pooling - (reply: 3)
  1357. stiching/linking/sewing pcr - (reply: 3)
  1358. Human mtDNA amplification problems - (reply: 3)
  1359. primer dimer - proplems (reply: 1)
  1360. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1361. Nested PCR - (reply: 2)
  1362. pfu vs long pcr mix - (reply: 7)
  1363. weird PCR ask for help - (reply: 12)
  1364. Methylation specific DMSO PCR - (reply: 1)
  1365. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  1366. No PCR product at all - (reply: 8)
  1367. PCR troubleshoooooting - primer dimer (reply: 1)
  1368. Primer efficiency test - (reply: 1)
  1369. primer software - (reply: 2)
  1370. Question on wired PCR - (reply: 1)
  1371. restriction digestion of PCR product - (reply: 7)
  1372. Primer design and Blast program at NCBI - comparison Primer3 and Primer Blast (reply: 1)
  1373. Checking PCR insert (into pGEMT vector) - (reply: 2)
  1374. How to know in which exon a primer match? - (reply: 1)
  1375. primer annealing - higher annealing= less wrong bounds (reply: 4)
  1376. Amplification of human genomic DNA - (reply: 2)
  1377. trouble amplifying 2.5kb product from genomic DNA - (reply: 3)
  1378. sequencing with forward/reverse primers - (reply: 7)
  1379. How to choose the parameters (Tm, cycli) for RT-PCR - (reply: 1)
  1380. PCR troubleshooting - two band after agrose electophoresis (reply: 4)
  1381. Adding A overhangs - primer design implications? - (reply: 1)
  1382. primer RE over-hang nucleotides - common sequences? (reply: 5)
  1383. Forward and reverse primers got very different Tm - what to do? (reply: 11)
  1384. Colony PCR screen positive - insert digestion negative - (reply: 2)
  1385. Q-PCR: Strange Amplification Curve shape (non exponential) - (reply: 3)
  1386. Finding cDNA for making a standard curve for real-time RT-PCR - (reply: 1)
  1387. primer design problem - (reply: 1)
  1388. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  1389. Problems with GOI Ct's - How to do PCR efficiency test if there is no Ct in GOI? (reply: 7)
  1390. Problem with primer efficiency analysis - (reply: 4)
  1391. slan real time PCR system for validation of microarray results? - (reply: 1)
  1392. PCR unusualband at ~230bps - PCR (reply: 1)
  1393. DMSO or BSA for PCR - (reply: 6)
  1394. Whole Genome Amplification - (reply: 2)
  1395. primer design - (reply: 1)
  1396. Settings op a RT-PCR, What is my next step - I`ve got RNA --> cDNA and working primers, what now? (reply: 1)
  1397. Separating PCR product on agarose gel with similar sizes - (reply: 5)
  1398. designing primers for genes not sequenced yet - (reply: 2)
  1399. Tris Buffer for PCR reaction - why? (reply: 1)
  1400. PCR a plasmid protein - (reply: 2)
  1401. multiplex PCR - (reply: 4)
  1402. Realtime PCR machine - (reply: 2)
  1403. PCR set-up calculation nightmares. - (reply: 3)
  1404. Primer desing - (reply: 2)
  1405. Colony PCR - (reply: 3)
  1406. Standard curves for PCR efficiency. - (reply: 2)
  1407. Increasing the number of products your PCR produces - Degenerative primers for multiple copy gene (reply: 3)
  1408. Question about RAPD PCR - (reply: 2)
  1409. Looking for help with my PCR! - (reply: 3)
  1410. How to incorporate dUTP when using WGA2 amplification for Affymetrix’s array? - (reply: 2)
  1411. Addition of A overhang for dummies? - How to add A overhangs for PCR product NOT made with proofreading Taq? (reply: 7)
  1412. primer design - (reply: 9)
  1413. problem with cloning PCR - can't amply the full-length cDNA with PCR (reply: 6)
  1414. PCR without thermal cycler? - (reply: 9)
  1415. primer contamination - primer contaminated with ice (reply: 3)
  1416. Loss of volume in PCR reaction in 96 well plate - (reply: 7)
  1417. RT-PCR problem - (reply: 4)
  1418. Only DNA ladder , No desired band in PCR - (reply: 4)
  1419. taq and PCR - (reply: 6)
  1420. Does purifying PCR probes for EMSA from EtBr gel interfere with binding? - (reply: 1)
  1421. PCR Efficiency - (reply: 2)
  1422. DNA and RNA contamination in RT PCR water controls - (reply: 2)
  1423. Long PCR and genomic DNA isolation problems - (reply: 2)
  1424. Colony PCR Question - Get the band i want but mini-prep shows no plasmids! (reply: 6)
  1425. NTC with specific amplification - (reply: 1)
  1426. Problem cloning bisulfite PCR BSP product - (reply: 6)
  1427. Opinions on Fermentas DreamTaq Green PCR Master Mix? - (reply: 3)
  1428. Primer3 vs Primer BLAST - (reply: 3)
  1429. PCR and then ligation - (reply: 7)
  1430. Primer design: free energy - (reply: 2)
  1431. Amplification in NTC and noRT controls - (reply: 5)
  1432. realtime PCR interpretation-peak found in negative control but no Ct value - (reply: 6)
  1433. Suggestions for optimizing a multiplex PCR? - Why do my bands keep disappearing in the positive control? (reply: 4)
  1434. asymmetric PCR - (reply: 1)
  1435. PCR amplifying 50bp ssDNA ? - PCR amplification (reply: 6)
  1436. CORRECT PCR Incorrect RTPCR - (reply: 10)
  1437. Is there a way to "rescue" an already-completed extraction from PCR in - (reply: 1)
  1438. trouble with pcr - (reply: 8)
  1439. Interpreting melting curve data in Sybr Green RT-PCR - (reply: 12)
  1440. Confusing bands from PCR - not primer dimer, not product (reply: 30)
  1441. Real time PCR results-interpretation - (reply: 1)
  1442. Real time PCR results-interpretation - (reply: 2)
  1443. Primer optimization for ChIP - (reply: 2)
  1444. sequencing primer: T7 or T7promoter? - (reply: 4)
  1445. 2-step or 3-step real time PCR - question about real time PCR (reply: 6)
  1446. Problems with my PCR's - Having an issue with some of my samples (reply: 2)
  1447. SYBR melting curve vs RT-PCR gel - (reply: 1)
  1448. primer tm - tm calculation for tagged primers (reply: 2)
  1449. Reusing 96 well plates for PCR - (reply: 2)
  1450. Suggestions for primer design - How many bonds is "too many" to avoid dimers and hairpins? (reply: 2)
  1451. PCR and AFLP - (reply: 1)
  1452. Universal 16s rRNA primers needed - (reply: 3)
  1453. Identifying PCR Inhibitors - (reply: 10)
  1454. PCR primers for histone mod ChIP - How to find the regions (reply: 1)
  1455. PCR + phusion enzyme = massive errors - (reply: 4)
  1456. long DNA amplification - (reply: 11)
  1457. same primer for reverse transcription and real time - is the primer same for the mRNA we start with and then for the cDNA al (reply: 2)
  1458. gDNA pcr product as standard for absolute quantification? - (reply: 1)
  1459. adding koazak sequence to primer - (reply: 1)
  1460. PCR for cloning - How to perform a PCR for cloning a gene? (reply: 5)
  1461. Designing primers for cloning - After primer designing, how should I perform the PCR? (reply: 15)
  1462. pcr trouble solved but I cant seem to understand why - (reply: 1)
  1463. Primer design if sequence is unknown for organism - (reply: 5)
  1464. adding C terminal tag to reverse primer - (reply: 1)
  1465. epitope tagging of pcr products - (reply: 1)
  1466. Is Taq polymerase still active after staying at 10C for one day? - (reply: 2)
  1467. Real time PCR info - (reply: 4)
  1468. Flox and Cre Primers - PCR Troubleshooting - Please Help (reply: 2)
  1469. PCR from BAC - PCR from BAC (reply: 1)
  1470. Designing Primers for RT-PCR after ChIP - Help to avoid primer dimers (reply: 2)
  1471. Failed TA cloning with Fusion PCR product! - I need help with TA cloning of an Overlap PCR product (reply: 1)
  1472. E. coli 16s - looking for specific primer+probe for E. coli (reply: 1)
  1473. How much product PCR?? - (reply: 3)
  1474. what primers should I use for DNA sequencing? please help - (reply: 2)
  1475. what primers should I use for DNA sequencing?? - (reply: 3)
  1476. Is my primer going to work for qRT-PCR? - (reply: 6)
  1477. Standard curve for RT-PCR - (reply: 1)
  1478. PCR using genomic DNA - (reply: 2)
  1479. pcr trouble!!! heeeeeellllpppppp!! - (reply: 1)
  1480. Problem amplifying plasmid - (reply: 1)
  1481. Sybr Green I in real-time PCR reaction - (reply: 3)
  1482. Primers annealing temperatures - (reply: 8)
  1483. Difficulties cloning by PCR - (reply: 5)
  1484. PCR Result Explanation (I needed you help) - Refer to the result, what is indicate A? (reply: 4)
  1485. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  1486. high Cp value PCR - (reply: 2)
  1487. real time PCR data presentation - presentation of delta CT and copy number (reply: 2)
  1488. Synthesize own Oligo-dT primers - (reply: 6)
  1489. Testing primer specificity... - ...without gDNA of target organisms (reply: 6)
  1490. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1491. dNTP or Deoxynucleotide solution Mix - (reply: 1)
  1492. Smearing/ no amplification in PCR - (reply: 3)
  1493. primer design - Primer design for PCR (reply: 1)
  1494. How are primers designed? - (reply: 4)
  1495. Source of DNA for a Reporter Construct - How to PCR the promoter region of interest? (reply: 1)
  1496. DNA methylated PCR - (reply: 2)
  1497. need help on primer calcuation soon - (reply: 1)
  1498. primer design - (reply: 6)
  1499. problem in pcr and purification - (reply: 3)
  1500. Inability to duplicate PCR results - (reply: 8)
  1501. Direct PCR (PCR without DNA extraction) - (reply: 2)
  1502. PCR using xt5 - (reply: 2)
  1503. long range PCR - purification of pcr frogament 9-13kb (reply: 9)
  1504. BSP PCR for help - no bands of tissue amplification (reply: 3)
  1505. Help! How to get rid of a repeatedly appearing smear in my RT-PCR result! - (reply: 2)
  1506. 2 bands or smear? 2-step RT-PCR - (reply: 1)
  1507. 23S rRNA Primer - need primer sequence (reply: 1)
  1508. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  1509. RT-PCR - problems with TaqMan PCR (reply: 2)
  1510. Problems with Phusion Polymerase (Finnzymes) - (reply: 3)
  1511. Multiplex PCR - Multiplex PCR and gel electrophoresis (reply: 1)
  1512. template for PCR for cloning purposes, miniprep or maxiprep? - (reply: 3)
  1513. Homogenizing animal tissue to RT-PCR in real-time - (reply: 2)
  1514. Small product amplification problems - (reply: 4)
  1515. multi way ligation or long range pcr? - (reply: 1)
  1516. Freezing primers+SYBR green - (reply: 2)
  1517. Tagged Primers - (reply: 5)
  1518. no pcr amplification product - (reply: 9)
  1519. Primer Dimer in NTC Only? - Primers Dimers show up in NTC but not template wells (reply: 4)
  1520. design primer for chip - (reply: 5)
  1521. How to quantify oligonucleotide primers using nanodrop ND-1000 software - newbie help (reply: 2)
  1522. PCR problem - (reply: 4)
  1523. differently behavioring replicates in real-time PCR - (reply: 1)
  1524. Multiplex PCR - (reply: 1)
  1525. Is necessary to use pfu polymearse for PCR amplification of pre-miRNA for clonin - (reply: 4)
  1526. No amplification in PCR using gDNA extracted from formailin fixed paraffin embed - (reply: 3)
  1527. Requirement of HPLC/PAGE purified primers in Quikchange mutagenesis - (reply: 3)
  1528. Volume of DNA to add to PCR Master Mix? - (reply: 1)
  1529. how much DNA you need for real time pcr after chip - CHIP (reply: 1)
  1530. PCR components storage - (reply: 3)
  1531. reconstitution of primers - (reply: 2)
  1532. No PCR bands with some templates - (reply: 5)
  1533. Mysterious PCR Contamination - (reply: 13)
  1534. BS PCR..please help - DNA methylation analysis (reply: 15)
  1535. RT-PCR Help - Protocol provided (reply: 4)
  1536. PCR product purification - (reply: 3)
  1537. Mutagenesis PCR and undesired amplification - (reply: 2)
  1538. Temp Gradient PCR - (reply: 2)
  1539. Vector sequence included in (PCR) amplified insert - (reply: 2)
  1540. PCR with genomic dna - (reply: 9)
  1541. primer dimer - (reply: 2)
  1542. PCR with long primers - (reply: 3)
  1543. BSP PCR standardization...help - (reply: 8)
  1544. PCR on degraded templates - (reply: 2)
  1545. PCR help needed - (reply: 3)
  1546. want to purify PCR product from agarose gel but have primer dimer - (reply: 3)
  1547. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  1548. light bands, dark smear, dark dimers - (reply: 2)
  1549. PCR, followed by sequencing... - why ?? (reply: 3)
  1550. PCR program - strange extension step (reply: 2)
  1551. Purifying dsDNA from ssDNA after a PCR reaction - (reply: 3)
  1552. Help me! How to design nested BSP primers? - (reply: 4)
  1553. REAL TIME PCR 7005 v 2.01 : I need more reference samples! - How to add more than one reference sample to Real Time?? (reply: 1)
  1554. Plasmid linearization by PCR - (reply: 4)
  1555. In-fusion advantage PCR cloning kit - --- failure after many many months-- (reply: 13)
  1556. Using BLAST to check primers - (reply: 17)
  1557. ExoSap then nanodrop? - Quantifying PCR products after exosaping! (reply: 2)
  1558. pcr doesnt show any band for DNa walking - (reply: 8)
  1559. storing large amounts of PCR mastermix beforehand - question (reply: 3)
  1560. questions about semi-quantitive PCR - weird result form semi-quantitive PCR (reply: 2)
  1561. Effects of more cDNA in PCR??? - (reply: 1)
  1562. PCR primer - (reply: 2)
  1563. PCR product disappears after restriction digestion - (reply: 4)
  1564. Primer design - GC clamp - (reply: 8)
  1565. Real Time PCR - excluding Ct - (reply: 2)
  1566. PCR product looks less intense compared to others - should I increase the number of cycles? (reply: 4)
  1567. Price of a real time PCR machine? - (reply: 4)
  1568. colony PCR - (reply: 8)
  1569. probe conc. of Asymmetric PCR - (reply: 1)
  1570. primer dimer or what? - (reply: 2)
  1571. PCR stopped working!!! HELP!!! - need results urgently (reply: 12)
  1572. Real Time PCR method comments - (reply: 2)
  1573. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  1574. colony pcr - (reply: 4)
  1575. Primers and Taqman Probes mixture question - (reply: 1)
  1576. truble shooting qiagen 1 step rt pcr kit - (reply: 2)
  1577. ligation troubleshooting - Trouble in inserting purified PCR product into pFlagCMV (reply: 4)
  1578. Not my insert size after colony PCR! - After colony PCR, the 'insert' size is 1kb more! (reply: 1)
  1579. Colony PCR - (reply: 15)
  1580. Trouble with PCR on genomic tomato DNA (I've tried many fixes) - (reply: 5)
  1581. no bands in my PCR - (reply: 9)
  1582. designing primers - (reply: 1)
  1583. Real Time PCR issues - Problems regarding my qPCR (reply: 6)
  1584. purefication and amplification of serum free DNA - (reply: 1)
  1585. How to use Real-time PCR to detect some gene copy numbe in plant genome? - (reply: 1)
  1586. Bisulfite Sequencing PCR - (reply: 4)
  1587. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  1588. way to know if my UNOII pcr machine is working properlly - (reply: 3)
  1589. Freezing DNA have effects on real-time PCR efficiencies - (reply: 1)
  1590. mRNA PCR - (reply: 1)
  1591. pcr product quantification - (reply: 5)
  1592. Negative Control Primers for ChIP Assay - (reply: 3)
  1593. Magnesium in PCR - (reply: 1)
  1594. PCR Sample Prep - (reply: 4)
  1595. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  1596. PCR from roots - I know this sounds a little crazy... (reply: 2)
  1597. Bizarre Contamination in PCR - (reply: 7)
  1598. PCR Cloning-large primers - (reply: 4)
  1599. Quantifying ingested bacteria without realtime PCR - (reply: 3)
  1600. forward and reverse primer - (reply: 4)
  1601. Check the primers - (reply: 4)
  1602. PCR problem from transformed TOPO TA vector - (reply: 3)
  1603. DIG DNA PCR labeling problem - (reply: 6)
  1604. PCR product stays on agarose gel well !! - I need help, what is it happening? (reply: 11)
  1605. BSP primers - (reply: 2)
  1606. Various size of insert after colony screen by PCR - (reply: 2)
  1607. inverse PCR molecular bilogy - (reply: 3)
  1608. PCR data analysis if the efficiencies aren't equal - real time PCR troubleshooting (reply: 2)
  1609. failed PCR on DNA extract from blood - (reply: 6)
  1610. Will ligase buffer affect polymerase fidelity? - (reply: 2)
  1611. Primer dimer issue in real time PCR - (reply: 21)
  1612. Leaky RT-PCR - (reply: 4)
  1613. Preparing PCR reactions from a master mix - Just a quick one (reply: 3)
  1614. PCR, RNA, Northern Blotting???? - (reply: 1)
  1615. bizzare PCR smear - why does science hate me (reply: 7)
  1616. RT-PCR primer dimers and cDNA degradation - (reply: 2)
  1617. PCR detection of SNP - (reply: 6)
  1618. PCR efficiency important in real time absolute quantification? - (reply: 4)
  1619. PCR contamination with human DNA first and now no bands!!! - (reply: 4)
  1620. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  1621. Sub-cloning sticky-end PCR products - (reply: 2)
  1622. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  1623. ChIP on chip amplification problems - (reply: 11)
  1624. Genotyping PCR - (reply: 3)
  1625. PCR screening of transformed bacterial colony - (reply: 6)
  1626. Is this standard valid? - pcr product as standards (reply: 5)
  1627. primer binding - (reply: 1)
  1628. weird ChIP primers! - (reply: 1)
  1629. PCR primers - (reply: 1)
  1630. Unwanted 100bp in RT-PCR - 100bp band appearing all the time after RT-PCR (reply: 7)
  1631. Slippage of PCR polymerame - How do I avoid it ? (reply: 6)
  1632. amplification in negative control in RT PCR - (reply: 2)
  1633. miRNA real time PCR by SYBR green methods - (reply: 1)
  1634. Deletion PCR - (reply: 9)
  1635. diluting to final primer concentration - please help correct (reply: 1)
  1636. Smear problem with my new primer set - (reply: 2)
  1637. PCR ghost bands - (reply: 1)
  1638. Amplifying a gene using a degenerate primer - (reply: 4)
  1639. DNA extraction and PCR advice requested - PCR, Contamination, Low abundance targets (reply: 7)
  1640. CP of real time PCR by LC480 - (reply: 1)
  1641. CHIP- Real Time PCR calculations - (reply: 1)
  1642. volume of DNA required (PCr product ) in agarose gel - (reply: 11)
  1643. transgene copy number - using real time PCR (reply: 1)
  1644. real-time PCR and HIV - what is the most commonly used assay to measure viral load in plasma (reply: 2)
  1645. Making Primer Dimers on Purpose - odd I know...but humor me (reply: 5)
  1646. Unspecific PCR - (reply: 7)
  1647. Real-Time PCR as a Microplate Reader - (reply: 1)
  1648. gene-specific RT-PCR - (reply: 4)
  1649. Primer Design - Design Primer with DNA Sequence (reply: 1)
  1650. RT-PCR Internal Standard - (reply: 2)
  1651. PCR arrays=Primers in a plate? - Can any PCR mix be used? (reply: 1)
  1652. Plasmid supercoiling affecting PCR? - (reply: 2)
  1653. RNA contamination in PCR - (reply: 1)
  1654. PCR without DNA extraction! - (reply: 2)
  1655. Primer tm - (reply: 1)
  1656. How to Clone a 2.3 Kb Gene with PCR - (reply: 2)
  1657. re-amplification in real-time PCR - (reply: 1)
  1658. primer design - really necessary? (reply: 2)
  1659. TaqMan real time PCR using abi 7700 system-troubleshooting - (reply: 5)
  1660. RT-PCR - (reply: 3)
  1661. Long product amplification (1598bp) - PCR for a product of 1598bp length (reply: 3)
  1662. Degenerative primers = multiple products? - (reply: 5)
  1663. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  1664. PCR problems on a ligation product - (reply: 5)
  1665. Genomic DNA and PCR - (reply: 4)
  1666. Primer dimers - shouldn't they also occur in the NTC? (reply: 5)
  1667. Sewing PCR - (reply: 3)
  1668. on the minimal pcr derived product - (reply: 2)
  1669. primer dimers - (reply: 5)
  1670. RT-PCR vs. conventional PCR - (reply: 1)
  1671. qPCR primer design - (reply: 5)
  1672. PCR and gel purification problem - did I screw this up (reply: 4)
  1673. Cloning PCR fragment - (reply: 1)
  1674. touchdown pcr - (reply: 6)
  1675. non specific pcr products - (reply: 4)
  1676. RT-PCR primer design - Intron/exon boundaries - (reply: 6)
  1677. Anyone with experiences in 2 step PCR - (reply: 1)
  1678. PCR works on lab strain but not patient sample - HIV related (reply: 4)
  1679. confirming concentration for primers in rt-pcr - RT-PCR, Primers (reply: 2)
  1680. DNA amplification but no bands on agarose gel, GC rich product, Roche - DNA amplification but no bands/smear in agarose gel (reply: 5)
  1681. Nucleic acid staining and PCR - Query onthe effect of nucleic acid stains on PCR (reply: 1)
  1682. re-amplification of a PCR product - is it recommended? (reply: 7)
  1683. mouse Gapdh chip-qPCR primers for control! - (reply: 7)
  1684. PCR machine with accurate temperature control? - (reply: 1)
  1685. Gene-specific RT-PCR - (reply: 2)
  1686. Primer stock confusion - (reply: 6)
  1687. VWR Real-Time PCR plates for eppendorf Realplex - (reply: 2)
  1688. primer design - primer design question (reply: 2)
  1689. Protocol for 16S rDNA real time PCR - (reply: 2)
  1690. Touchdown PCR - (reply: 1)
  1691. Determine annealing temperature of primers - (reply: 14)
  1692. Blunt ligation with PCR: is kinase needed? - is it necessary to phosphorylate if the vector is not CIAPed? (reply: 11)
  1693. polymerase for BSP - (reply: 2)
  1694. real time PCR primer designing problems,again - is NCBI's primer blast believable enough? (reply: 3)
  1695. Validating real time pcr primers - (reply: 2)
  1696. BSP,MSP primer design? - (reply: 1)
  1697. low gc primers - help with pcr using low gc primers (reply: 6)
  1698. Normalization for RNA or cDNA during two step RT-PCR? - (reply: 17)
  1699. Primers and annealing temperature - (reply: 2)
  1700. Good RT-PCR amplication but NO amplification with semi-quantitative PCR!? Wh - (reply: 3)
  1701. Copy number calculations in real time PCR - (reply: 2)
  1702. 4 degree hold in PCR machine - Does this hurt the machine? (reply: 3)
  1703. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1704. RT-PCR Negative controls - (reply: 1)
  1705. WHich primer to use for sequencing - (reply: 5)
  1706. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  1707. minimum length for the gene to be amplified in PCR - (reply: 6)
  1708. dNTP Quantity - (reply: 3)
  1709. annoying PCR cloning problem (season 2) - (reply: 5)
  1710. PCR with 60 bp primers - results in no product (reply: 6)
  1711. Amplification dwindles using little template RT-PCR - (reply: 3)
  1712. Running gel after RT-PCR - (reply: 5)
  1713. Degenerate PCR Size Limit? - (reply: 3)
  1714. primer design for pBAD topo expression kit - (reply: 2)
  1715. PCR problems.. - problems with conventional PCR (reply: 3)
  1716. 18S as a housekeeping gene for RT-PCR? getting wide variation - (reply: 2)
  1717. gDNA or cDNA amplification in RT-PCR - (reply: 3)
  1718. small PCR products on agarose gel? - (reply: 8)
  1719. Dnase digestion and PCR - (reply: 4)
  1720. Why is it necessary to add Taq DNA polymerase last during PCR? - (reply: 8)
  1721. primers fpr sequencing - (reply: 4)
  1722. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1723. Need an UV260 RT-PCR instrument - (reply: 1)
  1724. Nested Relative Quatitative Real-Time RT-PCR - (reply: 5)
  1725. Any mRNA amplification kit for microarray? - I have very little amount of RNA but have to submit it for microarray. (reply: 4)
  1726. PCR produces products of wrong size - (reply: 4)
  1727. PCR with pfu and degenerate primers (?) - (reply: 7)
  1728. a doubt about PCR gel purification - (reply: 4)
  1729. Draw PCR primer locations - PCR primer (reply: 4)
  1730. how to design PCR site-directed multiple mutagenesis - (reply: 1)
  1731. RT-PCR primers on coding sequence or UTRs? - (reply: 4)
  1732. Program for mapping primers to gene - diagram maker, visualize - (reply: 4)
  1733. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1734. PCR+enhancer - (reply: 4)
  1735. Serious issues with RT-PCR - (reply: 7)
  1736. PCR [dNTP] - dNTP concentrations (reply: 2)
  1737. PCR issue - (reply: 2)
  1738. dNTP question - (reply: 3)
  1739. PCR - (reply: 1)
  1740. long PCR primer - (reply: 7)
  1741. Taqman rtPCR primer and probe design - (reply: 3)
  1742. sequencing problem after pcr - (reply: 4)
  1743. Amplifying plasmid - non specific binding of primer - (reply: 3)
  1744. Help with Real Time PCR Well To Well Variation - (reply: 10)
  1745. primer design - (reply: 3)
  1746. Random hexamer vs oligo dT vs gene specific primer for RT - which do you use most? (old and useful thread) (reply: 2)
  1747. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  1748. Analysis of ChIP RT-PCR data - (reply: 7)
  1749. Make construct so it is only thing that can PCR after transfection - (reply: 2)
  1750. Negative flouroscence in real time PCR - (reply: 2)
  1751. Minimum number of PCR cycles to see a product? - (reply: 6)
  1752. decontamination - decontamination for PCR (reply: 6)
  1753. Troubleshoot ARMS PCR - (reply: 2)
  1754. negative control for PCR - (reply: 1)
  1755. how to design the primers for Real-Time PCR?? - I can't find the proper ones... (reply: 7)
  1756. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  1757. pcr pdt amplification - (reply: 1)
  1758. real time PCR - (reply: 3)
  1759. PCR fails when I scale up - (reply: 2)
  1760. can we use one-step RT-PCR kits to amplify DNA? - (reply: 1)
  1761. PCR bands in NTC Control and Neg Control Lanes - (reply: 4)
  1762. PCR not Working = SMEAR - (reply: 3)
  1763. How to do PCR fragment in 2 steps - (reply: 3)
  1764. Difference in Primer Melting Temperatures - Maximum allowable Tm difference? (reply: 4)
  1765. where to buy rotorgene real-time rt-pcr kits? - (reply: 4)
  1766. Real-Time RT-PCR one-step and two step issue - (reply: 13)
  1767. RT-PCR - problem of PCR (reply: 1)
  1768. PCR product longer than template - why? - (reply: 2)
  1769. Problem with Colony PCR - Problem with gel for colony PCR (reply: 3)
  1770. Dye recipe that can be used for PCR MM - (reply: 3)
  1771. Primers Not working - Real time PCR using SYBR Green (reply: 3)
  1772. PCR conditions with three primers - (reply: 1)
  1773. Real-Time PCR primer vs conventional PCR primer - (reply: 5)
  1774. distance between probe and primers in Taqman method - how far should they be? (reply: 2)
  1775. Cloning a labeled PCR product - (reply: 1)
  1776. PCR and cloning - (reply: 14)
  1777. QuantiTect Primers with Roche SYBR - ....can you mix and match? (reply: 2)
  1778. PCR product - not suppose to be there (reply: 7)
  1779. using the meth primer - (reply: 2)
  1780. Generating primers for MSP - (reply: 1)
  1781. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  1782. real time RT-PCR, 1-step vs. 2-step method - (reply: 2)
  1783. PCR from a smear genomic DNA ? - (reply: 5)
  1784. Primer design and need help - (reply: 11)
  1785. Setting up a multiplex PCR assay. - (reply: 2)
  1786. Programme for degenerate primer design - Programme for primer design (reply: 9)
  1787. Negative Control for ChIP realtime PCR in Mouse - (reply: 12)
  1788. cDNA and RT-PCR - (reply: 8)
  1789. Cloning purified PCR products eluted in Qiagen's EB - Concentrate a purified PCR product eluted with EB? (reply: 11)
  1790. Proofreading polymerase problem - Anyone experienced similar problems? (reply: 5)
  1791. PCR problem - help me plsss (reply: 9)
  1792. gene-specific qPCR primers for a multigene family - primer design (reply: 5)
  1793. Important literature for real time PCR users - Important documents for beginners as well as advanced users (reply: 41)
  1794. PCR efficiencies - (reply: 4)
  1795. RT-PCR primer design guide - How to check gene structure and design the primer? - Recovered post (reply: 4)
  1796. PCR quantification - (reply: 1)
  1797. PCR quantification - (reply: 1)
  1798. Long PCR product - (reply: 9)
  1799. problem in Pcr amplification - (reply: 7)
  1800. What does 'limiting dilution PCR' mean? - I wanna know the meaning of 'limiting dilution' (reply: 4)
  1801. difference between hotstart and taq polymerase - (reply: 7)
  1802. sequencing the pcr product - (reply: 15)
  1803. Pcr amplification - primer designed from UTR regions (reply: 5)
  1804. RT-PCR - can you keep the PCR plate in the fridge? - Please help! (reply: 1)