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primer check!!! - (Mar/24/2010 )

hello everybdy....

i have got a problem regarding primer check....ive got two pair of primers but unfortunately dont knw they are specific for which loci.....can any1 help me how to check that... i was wondering if i could BLAST them..but dont knw how to BLAST it..so is BLSAT the answer..if yes then how to BLAST it...?

-Bilal Malik-

Bilal Malik on Mar 25 2010, 12:56 PM said:

hello everybdy....

i have got a problem regarding primer check....ive got two pair of primers but unfortunately dont knw they are specific for which loci.....can any1 help me how to check that... i was wondering if i could BLAST them..but dont knw how to BLAST it..so is BLSAT the answer..if yes then how to BLAST it...?


Hi, you can use NCBI's primer blast software...

-DRN-

dont knw how to use it!!! have tried it :rolleyes:

-Bilal Malik-

:rolleyes: go to primer blast, put in ur forward, reverse primer sequences and the organism, against whose genome, u want to do the BLAST..........whats the problem that u were facing????

-DRN-

problem is that i dont knw for which organism these primers actually are. The only thing i knw is the sequence of the primers which is written on the voile...

-Bilal Malik-

so how can i BLAST it without knowing the organism....

-Bilal Malik-

by default, the organism name is set to human beings, u just remove that and leave the space blank.....

-DRN-