Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

Design primer from incomplete sequence... - (Mar/04/2013 )

Hi guys....

Im new here and i really really really need your help... My research is about to complete the incomplete sequence of phenylalanine ammonia lyase gene from a plant.....i already have the incomplete sequence of the supervisor asked me to design primer from the project involve RACE, RTPCR and genome walking...I really dont know how to design primer from incomplete sequence of gene...i hope you guys can help me...

-Saiful Shakirin-

If you have sequences for this gene in other species you can align them all and take the "consensus" sequence and use that for designing primers.


Align with other sequnces by using blastn from NCBI is it? how to know which part of sequence i need to choose to design primers?

-Saiful Shakirin-