pcr amolification with long pcr enzyme mix for cloning - (Feb/07/2010 )
Hi
I am going to clone a 1.8 kb gene is it safe to amplify it with long pcr mix (fermentas) or high fidelity enzyme mix (roche) or I should aplify it only with pfu?
regards
-sara.r-
sara.r on Feb 7 2010, 03:27 PM said:
Hi
I am going to clone a 1.8 kb gene is it safe to amplify it with long pcr mix (fermentas) or high fidelity enzyme mix (roche) or I should aplify it only with pfu?
regards
I am going to clone a 1.8 kb gene is it safe to amplify it with long pcr mix (fermentas) or high fidelity enzyme mix (roche) or I should aplify it only with pfu?
regards
Hi,
pfu should be quite ok for your purpose.
-DRN-