smears in PCR products (bisfulite treated DNA) - (May/12/2011 )

Hi Everyone,

I've successfully amplified my products with:

Zymo research's "human methylated DNA".

However, when I extract DNA from cell culture, I don't see any products- only smears. I've designed my primers using methylprimer express.

I do 40 cycles.

What does this mean?

Thanks.

You got expected products using control templates, suggesting the primers work. Make sure your DNA quality (purity) and recovery are good. You may also try hot start taq polymerase such as JumpStart redTaq from sigma.

pcrman on Sun May 15 16:56:39 2011 said: