urgent help plz with RT-PCR - (May/07/2012 )
Can any ONE have experience in RT PCR tell me how the measure the over expression of mRNA by using housekeeping gene ? do u measuring it by automated machine or you have to ues agrose gel. what is the best to do to measure the quantitative of mRNA?
It is best to use Real-Time PCR machines for this kind of measurement.
when u uesing housekeeping gene , do i need to use sypree green dey or taqman? plz help me bcz i do not have any exp.
When you have no experience with real-time, best way is to first search and read something about it.
You can start with this forum topic - Important literature for real time PCR users
You can then ask about specific things you don't completely understand.