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PCR AMPLIFICATION - (Aug/02/2010 )

HI FRIENDS
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.

-PRATIPAL-

PRATIPAL on Tue Aug 3 05:15:18 2010 said:


HI FRIENDS
I AM A PLANT MOLECULAR BIOLOGY STUDENT. I HAVE PREPARED THE GENE SPECIFIC PRIMERS. MY PROBELEM IS THAT I GOT AMPLIFICATION WITH cDNA BUT UNABLE TO GOT FROM THE GENOMIC DNA. i AM UNABLE TO TROUBLESHOOT THIS.


Did your primer spans the intron region, and only amplified the spliced exon?

-adrian kohsf-