How to know in which exon a primer match? - (Jan/07/2010 )
I am a beginner in RT-qPCR. And I have a simple question. When you have a couple of primer or at least, one primer, how do you proceed to know in which exon your primer matches?
I have a primer sequence that match on the gene of interest (BLAST results from ncbi). From here, OK. Then I click on the "gene ID" from the accession number, I get the FASTA from my gene on interest, OK! But How can I know in which exon of this gene my primer matches? (i.e. this primer fit on the exon n°6 from the gene A between 1245 and 1265).
I do not know if I am clear.
Thank you for your answers!!
For your question, Primer-check is what you need at http://www.tigerteamconsulting.com/SpliceCenter/PrimerCheck