Designing cloning primers for DNMT - (May/22/2013 )
I am having a little a little bit of trouble with designing methyltransferase primers. I have conducted a blastp and obtained my sequence of interest, but I am confused as to what I should do as far as creating my primers by using the CDS (coding sequence) or the whole genomic sequence. In order to clone the full length DNMT. PLEASE HELP!!!!!
THANK YOU in advance
Which organism and which DNMT? There are several DNMT genes. please specify.
So far I have found CMT's, MET's and DRM's in phaseolus using bioinformatic tools.