good 16S univ. primer for qPCR? - (Jan/29/2013 )

Hi! Would you please suggest some good 16S univerisal primers for qPCR? I have difficult finding one and need it very soon.
I have tried a 466bp one, and it maybe too big.

Thanks a lot!

check Qiagen website, you need shorter amplicons, below 150 bp is desirable.

Thanks! Do you mean search "16S" in the "QIAGEN Reference Database"? That comes only 3 articles that using their reagents. Do you mean Qiagen website have other better way to look for primers? I appreciate your help!

Curtis on Wed Jan 30 04:33:25 2013 said:

yes, since you are doing qPCR you can use their Quantitect products. I used 2-3 years ago and they worked really well. Just search their website for 16s.