primers with restriction site - (May/27/2010 )
HI,
I need to design primers with restriction site to clone the entire reading frame of my gene of interest into a vector. I understand I have to add a kozak sequence before the start codon. I know the function of the kzak sequence, but what specific sequence is added? Is it GTC?
-SF_HK-
I use ACCATGG where the ATG is the start codon.
-TomH-