# dNTP Quantity - (Apr/10/2009 )

Hi Everybody,

how do we count numbers(how many) of "A" in 1mM dATP solution?

Actually i wanna know that, can we theoritically able to calculate the actual amount of dNTPs(in numbers) required for 30 cycles in PCR? if yes then please tell me.

Thanks

-pinokyo-

pinokyo on Apr 10 2009, 01:55 PM said:

Hi Everybody,

how do we count numbers(how many) of "A" in 1mM dATP solution?

Actually i wanna know that, can we theoritically able to calculate the actual amount of dNTPs(in numbers) required for 30 cycles in PCR? if yes then please tell me.

Thanks

how do we count numbers(how many) of "A" in 1mM dATP solution?

Actually i wanna know that, can we theoritically able to calculate the actual amount of dNTPs(in numbers) required for 30 cycles in PCR? if yes then please tell me.

Thanks

A 1 M solution of anything dATP or otherwise is defined as having 6.023 x10^23 molecules (Avagadro's Number). Therefore, a 1 mM solution has 1000x fewer molecules = 6.023 x10^20 molecules.

-Dr Teeth-

That's the number of molecules in one milli- Mole, not the number in a 1 mM solution, which is 1 milli-mole per liter. You need to tell us the volume to determine the number of molecules. So, a 10 ul reaction with 1 mM dATP would have 10**-5 times fewer molecules, or 6 x 10**15 molecules.

-phage434-

Dr Teeth on Apr 10 2009, 11:59 PM said:

pinokyo on Apr 10 2009, 01:55 PM said:

Hi Everybody,

how do we count numbers(how many) of "A" in 1mM dATP solution?

Actually i wanna know that, can we theoritically able to calculate the actual amount of dNTPs(in numbers) required for 30 cycles in PCR? if yes then please tell me.

Thanks

how do we count numbers(how many) of "A" in 1mM dATP solution?

Actually i wanna know that, can we theoritically able to calculate the actual amount of dNTPs(in numbers) required for 30 cycles in PCR? if yes then please tell me.

Thanks

A 1 M solution of anything dATP or otherwise is defined as having 6.023 x10^23 molecules (Avagadro's Number). Therefore, a 1 mM solution has 1000x fewer molecules = 6.023 x10^20 molecules.

Thanks

Thank u very much

-pinokyo-