Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

PCR buffer without Tris - (Jan/23/2012 )

Hi Guys

I am trying to do a PCR.I need to conserve the pH changes so I want to make a buffer without Tris ,so that H-ion is not buffered...I have had papers regarding this..but as they have mentioned its not working.I have tried many times ,its failure always.I make buffer with ph 8.

Any sugggestions?


-Priyanka Rathi-

what are you using instead of Tris? most of buffers ruins your PCR