Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

adding koazak sequence to primer - (Oct/27/2009 )

Hi
I know the consensus kozak sequence is gccgcc(a/g)ccAUGg but the second codon in my gene is started with a not g. should I convert it to g to make it identical to kozak or using just gccgcc(a/g)ccAUG is OK?
best

-sara.r-

Don't convert the G, it should be fine without it.

-bob1-