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Designing a PCR based detection method for an unknown virus/pathogen - (Jul/16/2013 )

Hi, can someone explain the steps one should follow to design a PCR based detection method to identify an unknown virus, or a novel serotypes of virus/ strains of bacteria etc. whose sequence information has not known yet?



I would do NGS sequencing


or using universal primers for e.g. bacteria for a first amplification and then sequencing this locus/these loci