distance between probe and primers in Taqman method - how far should they be? (Feb/21/2009 )
I know that Taqman probes should not overlap with the primers when doing real-time pcr, but can you please tell me how far the probe and primers could be located from each other so that the PCR is efficient?
Curtis on Feb 21 2009, 12:00 PM said:
I don't know but I generally keep at least 7-10 nt distance on both sides, and most of my primer-probe combos have worked.
The primers should be designed as close as possible to the probe without overlapping it.