distance between probe and primers in Taqman method - how far should they be? (Feb/21/2009 )
I know that Taqman probes should not overlap with the primers when doing real-time pcr, but can you please tell me how far the probe and primers could be located from each other so that the PCR is efficient?
-Curtis-
Curtis on Feb 21 2009, 12:00 PM said:
I know that Taqman probes should not overlap with the primers when doing real-time pcr, but can you please tell me how far the probe and primers could be located from each other so that the PCR is efficient?
I don't know but I generally keep at least 7-10 nt distance on both sides, and most of my primer-probe combos have worked.
-TanyHark-
The primers should be designed as close as possible to the probe without overlapping it.
-chrisbelle-