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Beta actin Primer designing - Will anyone plz send me the sequence of primers for rat beta-actin (Sep/13/2010 )

Hay,

I am not getting my beta-actin pcr as insilico pcr and wet lab pcr beta actin length is different.
If someone can help me with this problem, I would be really thankful. If I have missed out some information, please feel free to ask.
Thanks in advance.

-akhilesh004-

akhilesh004 on Mon Sep 13 10:58:53 2010 said:


Hay,

I am not getting my beta-actin pcr as insilico pcr and wet lab pcr beta actin length is different.
If someone can help me with this problem, I would be really thankful. If I have missed out some information, please feel free to ask.
Thanks in advance.


You mean to say that the primer you designed using primer3 like softwares is giving you an amplicon size which is different from the theoretical amplicon size?

-baccilus-

baccilus on Mon Sep 13 11:16:22 2010 said:


akhilesh004 on Mon Sep 13 10:58:53 2010 said:


Hay,

I am not getting my beta-actin pcr as insilico pcr and wet lab pcr beta actin length is different.
If someone can help me with this problem, I would be really thankful. If I have missed out some information, please feel free to ask.
Thanks in advance.


You mean to say that the primer you designed using primer3 like softwares is giving you an amplicon size which is different from the theoretical amplicon size?


Yes, the band of primer3 is 336 bp and i am getting 250 bp in wet lab PCR amplification.

-akhilesh004-