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Direct PCR (PCR without DNA extraction) - (Sep/23/2009 )

Hi people,
I have one question about Direct PCR. I want to ask if anybody using direct PCR for SNP detection but without commercial kit. I tried to boil samples 15 minute at 100 C and than i used in my PCR but i did not any results of my PCR product. I used cells from buccal swabs. I know that is some a commercial kits for direct PCR but I want to try this method without this kits and I want to request anybody for any simple guidance. Thank you very much for your help.

Miso :unsure:

-miso-

Try to boil samples 5 minutes in 0.2M NaOH.

This technique is reported to be useful on plants and their cell walls are much stronger than human so I guess it may working.

http://www.ingentaconnect.com/content/klu/...000002/05112630

-gleb.kudr-

gleb.kudr on Sep 26 2009, 08:04 AM said:

Try to boil samples 5 minutes in 0.2M NaOH.

This technique is reported to be useful on plants and their cell walls are much stronger than human so I guess it may working.

http://www.ingentaconnect.com/content/klu/...000002/05112630


Thank you for your help. I will be try this idea. Thank

-miso-