What is the maxium amount of glycerol can be added in a PCR reaction? - (Nov/28/2011 )

If the templates are in glycerol? Thanks.

-oneonenine-

Total final concentration of more than about 5-10% will cause problems.

-phage434-

phage434 on Tue Nov 29 01:25:47 2011 said:

Total final concentration of more than about 5-10% will cause problems.

Thanks. Any method to remove the glycerol?

-oneonenine-

well diluting your template is one way to go.......

-lamaksha77-