Primers too dilute - (Apr/18/2012 )
I am performing PCR with primers on a gene that is about 10kb long. I am not getting any results from my gel, all I see is a smear. I believe that my primers are too diluted, but I am not sure how to go about solving this. Anyone have any ideas? Thanks!
What concentration you use then? Stock solutions usually have 100 pmol/microl and working solutions e.g. 10 pmol/microl that are then diluted to changing final concentrations in the PCR mix.
And for 10 kb you perhaps to try a long range PCR approqach that many companies offer (if you're not using it already)...