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total cDNA amplification by PCR - (Mar/13/2011 )

hi everyone,
i have isolated total rna from an animal tissue and done the first strand synthesis (cDNA). but now i don't have isolated rna and i only have a very small amount of cDNA. so can i amplify that cDNA by PCR using random primers and using a lower annealing temperature?


It is possible to amplify cDNA for expression analysis. You can read the following papers to know more

High-copy cDNA amplification of minimal total RNA quantities for gene expression analyses.
Simple method for cDNA amplification starting from small amount of total RNA.


pcrman on Sun Mar 13 07:19:09 2011 said:


I'm new to this subject......