What products will I get from this pcr? - (Jan/24/2012 )
I need to assemble 2 sequences from genomewalking PCR which have an overlap of 100b and contain the same adaptor sequence. A senior told me that a high fidelity reaction without primers should work but I am still confused which possible products will I get here? And any suggestions for setting up such PCR?.
50b Adaptor sequence - 1000b Sequence 1 - 100b overlap
50b Adaptor sequence - 100b overlap - 1500b Sequence 2
as your sequence are not too short you'd better to use primers.