could you help me with my stem loop RT primer? - (Jun/25/2014 )

I use U6 as internal control, and i found U6 sequence :

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGUG AAGCGUUCCA UAUUUU

And i design the stem loop RT primer like this :GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATA

and I use this three pairs of pimer to do the qPCR (SYBR GREEN )

U6 F1 CTCGCTTCGGCAGCACA

U6 R1 AACGCTTCACGAATTTGCGT

U6 F2:GCGCGT CGTGAAGCGTTC

U6 R2: GTGCAGGGTCCGAGGT

U6 F3: CGG GTGCTCGCTTCGGCAG

U6 R3: ATCCAGTGCAGGGTCCGAGG

But it could not be amplified.

The stem loop RT primer had been re-fold. And I have try different reverse transcriptase condition like 65℃ 10mins 42℃ 30mins or 16℃ 30mins 42℃30mins or 30℃ 10mins 42℃ 30mins. both of them do not work.

Could you please give me some suggestion? thank you

-superice-

the qpcr condition is 95℃ 15s 60℃ 40s 40cycle thanks

-superice-