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pcr - problem in pcr (Dec/24/2010 )

I started pcr for my thesis last month and I can got an acceptable reply from it.But now it dont work and I dont know what is the problem?
does Anybody help me?


Can you tell us more about this PCR? Are you using a different DNA template?

When problems like this happens it usually is caused by the template degrading.


Very thanks. My object is investigation genetic polymorphism in Iranaian population and I have different dna from hearty sicks.