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long fragment PCR - (Nov/07/2012 )

Hi, I'm a master degree student of biotechnology. I want to amplify 5 kb fragment of mouse genomic DNA, I used Accuprime pfx that belongs to invitrogen company, but i didn't see any band in electrophoresis gel. I've changed everything but it doesn't work. whould you please what should i do?

Thanks a lot.


Can't help without knowing LOTS more than you have told us. Try again.


Yes, please provide more information regarding your PCR condition, template, etc.