Designing primer to remove his-tag - (Dec/02/2010 )
Inorganic chemist trying to do biochemistry here....
I have a protein that is his-tagged at both the N- and C-terms. I want to get rid of the his-tags and just amplify the gene of the wild-type. There is no type of cleavage site after the his-tag. How would I design a primer that removes the his-tag from either end? Should I use an internal primer?
I would suggest you buy a new expression vector, re-clone the gene again with stop codon.