colony PCR for subcloning gene - (Sep/08/2010 )
I want to subclone a gene from the E coli genomic DNA into an expression vector. Can I simply do a colony PCR with primers containing the restriction sites to amplify the gene or do I have to purify the genomic DNA first? Thanks!
If your primers produce a single well-isolated band of sufficient quantity when used in colony PCR, you can recover this band from a gel and clone it.