Protocol Online logo
Top : New Forum Archives (2009-): : DNA Methylation and Epigenetics

negative control for msp pcr - (May/23/2011 )

We start a new methylation specific project. Any suggestion for the best negative control DNA (unmethylated DNA), for the methylation specific pcr? It suggested that we should use placental DNA. Any alternative choice?


we buy unmethylated dna from milipore.


Placental DNA is good. You can also try DNA extracted from sperm or whole-genome-amplified DNA (Qiagen-kit).