Which High-Fidelity polymerase is better? - (Mar/08/2013 )
I need to amplify 7 kb sequence with high fidelity. We have three high-fidelity polymerases in our lab:
PfuUltra High-Fidelity DNA polymerase (from QuikChange II site directed mutagenesis kit, Agilent)
KAPA Hifi HotStart DNA polymerase (Kapabiosystems)
Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs)
Naturally, each manufacturer claims that his product is the best one. I have no experiences with HF polymerases so would it be possible to advise me which polymerase is really the best, please? Thank you.
My go-to polymerase is Q5. PfuUltra is somewhat tricky, no experience with Kappa.
Kapa is no good compared to the other ones. best options - Q5 (NE biolabs) and Phusion high fidelity DNA polimerase (from ThermoFisher) they are both very good, but it depends on the system of the reaction which one is better. but for 7kb i guess Phusion is better.