improving sensitivity of one step TAQMAN REal time PCR - (Aug/15/2012 )
More efficient primers.
In one step, is there also an option to degrade RNA after reverse transcription? Sometimes the RNA can inhibit reaction.
Also in this respect, maybe try some additives, that may overcome such inhibition problems, HDV has higher GC content in some areas so maybe some secondary structure relaxing aditives like DMSO could also help.