PCR of microRNA first strand cDNA - (Apr/19/2011 )
If I have first strand cDNA synthesized for microRNA, and run it through PCR- is it possible to get double stranded cDNA if I put both the reverse and forward primers for the two strands?
why cannot you get double stranded cDNA??
is there any possible you cannot get that?
maybe i just don't figure it out