primer and promoter sequences - (Dec/08/2010 )
I'm new plant transformation and now I have to isolate RNA(ARR22 gene, At3g04280) from the Arabidopsis. I'm going to do rt-pcr where I have to find the sequence for the promoter and the primer. Please anyone guide me on how to get the sequence. I already try it using primer3 and PlantCare(promoter sequence), but I'm not sure whether they are the right sequences or not. Besides, the sequences that I have are difference from the previous study. I also have to find the sequence for tomato plant (SL1.00sc06004_225.1.1)(homologues with Arabidopsis ARR22 gene).
Try ensembl DB. It worked fine for me
Hope to be helpful