transgene copy number - using real time PCR (Jun/01/2009 )
Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?
I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.
I have never done real time PCR as well but use PCR very often in my project.
Thanks for your help!
-res_org-
res_org on Jun 1 2009, 11:20 PM said:
Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?
I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.
I have never done real time PCR as well but use PCR very often in my project.
Thanks for your help!
I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.
I have never done real time PCR as well but use PCR very often in my project.
Thanks for your help!
I have been trying to get my Southern blot to work for a while
So I looked up the RT- PCR way and found this link. Maybe you can look through it. I am not planning to buy the kit, but to use my GFP primers. Maybe you could work out something similar 
http://catalog.takara-bio.co.jp/en/product...itid=U100006070
-zodiac1505-