no or poor amplification - (Mar/02/2010 )
Hi all,
I used same cdna template.. but when i optimize my primer using conventional pcr method, i did annealing temp adjustment and mgcl optimization. the problem is sometime i got pretty nice bands, but sometimes i got poor amplification or even no amplification, even i used same primer stock, same mgcl2 and everything is the same. it's really odd how could this occur. any advise? thanks
-wntiong-
Poor cDNA quality, i would say
wntiong on Mar 2 2010, 03:51 AM said:
Hi all,
I used same cdna template.. but when i optimize my primer using conventional pcr method, i did annealing temp adjustment and mgcl optimization. the problem is sometime i got pretty nice bands, but sometimes i got poor amplification or even no amplification, even i used same primer stock, same mgcl2 and everything is the same. it's really odd how could this occur. any advise? thanks
I used same cdna template.. but when i optimize my primer using conventional pcr method, i did annealing temp adjustment and mgcl optimization. the problem is sometime i got pretty nice bands, but sometimes i got poor amplification or even no amplification, even i used same primer stock, same mgcl2 and everything is the same. it's really odd how could this occur. any advise? thanks
-susanna-