PCR colonies of Pichia pastoris - (Sep/26/2011 )
Any of you know a protocol to perform a rapid colony PCR of Pichia pastoris?, I think is necessary to use liticase, because at least in my hands it is not possible to perform a colony PCR as in the case of E. coli clones, with a previous step heating the colonies.
Thank you in advance!!
This method works very well: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182553/