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PCR colonies of Pichia pastoris - (Sep/26/2011 )

Hello all!
Any of you know a protocol to perform a rapid colony PCR of Pichia pastoris?, I think is necessary to use liticase, because at least in my hands it is not possible to perform a colony PCR as in the case of E. coli clones, with a previous step heating the colonies.
Thank you in advance!!


This method works very well: