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how to hasten real-time PCR amplifications - (Mar/05/2014 )

hello there

when I perform real-time PCR for different genes in human cancer cells, I usually got a base-line until about 25 cycles then linear amplifications starts. I use the following conditions:

1) cDNA concentration/well ~29 nanograms

2) forward or reverse primers concentration : 350 nM (final)


How can I shorten the length of the base-line and get amplifications earlier after about 15 cycles?

thanks in advance


In principle with a higher cDNA amount


seconding the more cDNA.